scholarly journals Metabolism-mediated mechanisms underpin the differential stomatal speediness regulation among ferns and angiosperms

2021 ◽  
Author(s):  
Silvio Cândido‐Sobrinho ◽  
Valéria Lima ◽  
Francisco Freire ◽  
Leonardo de Souza ◽  
Jorge Gago ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Carbon Allocation ◽  
Stomatal Closure ◽  
Growth Stress ◽  
Primary Metabolites ◽  
Trade Offs ◽  
Time Period ◽  
Closure Mechanism ◽  
Stomatal Movements ◽  
Metabolomics Analysis

Recent results suggest that metabolism-mediated stomatal closure mechanisms are important to regulate differentially the stomatal speediness between ferns and angiosperms. However, evidence directly linking mesophyll metabolism and the slower stomatal conductance (gs) in ferns is missing. Here we investigated the effect of exogenous application of abscisic acid (ABA), sucrose and mannitol on gs kinetics and carried out a metabolic fingerprinting analysis of ferns and angiosperms leaves harvested throughout a diel course. Ferns stomata did not respond to ABA in the time period analysed. No differences in the relative decrease in gs was observed between ferns and the angiosperm following provision of sucrose or mannitol. However, ferns have slower gs responses to these compounds than angiosperms. Metabolomics analysis highlights that ferns have higher accumulation of secondary rather than primary metabolites throughout the diel course, with the opposite being observed in angiosperms. Our results indicate that metabolism-mediated stomatal closure mechanism is conserved among ferns and angiosperms and that the slower stomatal closure in ferns is associated to a reduced capacity to respond to mesophyll-derived sucrose and to a higher carbon allocation toward secondary metabolism, which likely modulates both photosynthesis-stomatal movements and growth-stress tolerance trade-offs.

Inhibition of cambial activity in Abies balsamea by internal water stress: role of abscisic acid

1975 ◽  
Vol 53 (24) ◽  
pp. 3041-3050 ◽  
Author(s):  
C. H. A. Little
Keyword(s):  
Abscisic Acid ◽  
Water Stress ◽  
Indoleacetic Acid ◽  
Stomatal Closure ◽  
Abies Balsamea ◽  
Cambial Activity ◽  
Inhibitory Effect ◽  
Internal Water ◽  
Endogenous Aba

In experiments with attached and detached shoots of balsam fir, Abies balsamea L., synthetic (±)abscisic acid (ABA) (1) reduced photosynthesis and transpiration by inducing stomatal closure, (2) inhibited indoleacetic acid (IAA) - induced cambial activity in photosynthesizing and non-photosynthesizing shoots, and (3) inhibited the basipetal movement of [14C]IAA. Neither gibberellic acid nor kinetin counteracted the inhibitory effect of (±)ABA on IAA-induced cambial activity. In addition it was demonstrated that increasing the internal water stress increased the level of endogenous ABA in the phloem–cambial region of bark peelings and decreased the basipetal movement of [14C]IAA through branch sections. On the basis of these findings it is proposed that internal water stress inhibits cambial activity, partly through increasing the level of ABA; the ABA acts to decrease the provision of carbohydrates and auxin that are required for cambial growth.

scholarly journals Arabidopsis NPF4.6 and NPF5.1 Control Leaf Stomatal Aperture by Regulating Abscisic Acid Transport

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 885
Author(s):  
Takafumi Shimizu ◽  
Yuri Kanno ◽  
Hiromi Suzuki ◽  
Shunsuke Watanabe ◽  
Mitsunori Seo
Keyword(s):  
Abscisic Acid ◽  
Plant Hormone ◽  
Leaf Surface ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Cell Types ◽  
Acid Transport ◽  
Wild Type ◽  
Vascular Tissues ◽  
Abscisic Acid Transport

The plant hormone abscisic acid (ABA) is actively synthesized in vascular tissues and transported to guard cells to promote stomatal closure. Although several transmembrane ABA transporters have been identified, how the movement of ABA within plants is regulated is not fully understood. In this study, we determined that Arabidopsis NPF4.6, previously identified as an ABA transporter expressed in vascular tissues, is also present in guard cells and positively regulates stomatal closure in leaves. We also found that mutants defective in NPF5.1 had a higher leaf surface temperature compared to the wild type. Additionally, NPF5.1 mediated cellular ABA uptake when expressed in a heterologous yeast system. Promoter activities of NPF5.1 were detected in several leaf cell types. Taken together, these observations indicate that NPF5.1 negatively regulates stomatal closure by regulating the amount of ABA that can be transported from vascular tissues to guard cells.

scholarly journals Interactions between leaf water potential, stomatal conductance and abscisic acid content of orange trees submitted to drought stress

2004 ◽  
Vol 16 (3) ◽  
pp. 155-161 ◽  
Author(s):  
Mara de Menezes de Assis Gomes ◽  
Ana Maria Magalhães Andrade Lagôa ◽  
Camilo Lázaro Medina ◽  
Eduardo Caruso Machado ◽  
Marcos Antônio Machado
Keyword(s):  
Abscisic Acid ◽  
Water Stress ◽  
Stomatal Conductance ◽  
Water Potential ◽  
Leaf Water Potential ◽  
Acid Content ◽  
Stomatal Closure ◽  
Leaf Water ◽  
Orange Trees ◽  
Abscisic Acid Content

Thirty-month-old 'Pêra' orange trees grafted on 'Rangpur' lemon trees grown in 100 L pots were submitted to water stress by the suspension of irrigation. CO2 assimilation (A), transpiration (E) and stomatal conductance (g s) values declined from the seventh day of stress, although the leaf water potential at 6:00 a.m. (psipd) and at 2:00 p.m. (psi2) began to decline from the fifth day of water deficiency. The CO2 intercellular concentration (Ci) of water-stressed plants increased from the seventh day, reaching a maximum concentration on the day of most severe stress. The carboxylation efficiency, as revealed by the ratio A/Ci was low on this day and did not show the same values of non-stressed plants even after ten days of rewatering. After five days of rewatering only psi pd and psi2 were similar to control plants while A, E and g s were still different. When psi2 decreases, there was a trend for increasing abscisic acid (ABA) concentration in the leaves. Similarly, stomatal conductance was found to decrease as a function of decreasing psi2. ABA accumulation and stomatal closure occurred when psi2 was lower than -1.0 MPa. Water stress in 'Pera´ orange trees increased abscisic acid content with consequent stomatal closure and decreased psi2 values.

scholarly journals Exogenous Abscisic Acid Application Effects on Stomatal Closure, Water Use, and Shelf Life of Hydrangea (Hydrangea macrophylla)

2009 ◽  
Vol 27 (4) ◽  
pp. 234-238 ◽  
Author(s):  
Marc W. van Iersel ◽  
Kate Seader ◽  
Sue Dove
Keyword(s):  
Abscisic Acid ◽  
Shelf Life ◽  
Water Uptake ◽  
Stomatal Closure ◽  
Ornamental Plants ◽  
Detectable Effect ◽  
Water Control ◽  
Available Water ◽  
Hydrangea Macrophylla ◽  
Plant Available Water

Abstract A lack of adequate watering reduces the shelf life of many ornamental plants during retail. Our goals were to determine whether sprays or drenches with abscisic acid (ABA) can reduce transpiration and extend the shelf life of hydrangea (Hydrangea macrophylla). During the first 5 days after treatment, ABA drenches of 125 to 1000 ppm reduced stomatal conductance (gs) by 50 to 80% as compared to water. ABA-induced stomatal closure reduced plant water uptake from the substrate; control plants took up half of the plant-available water during the first 7 days after treatment, while it took 14 days for plants drenched with 1000 ppm to take up half of the available water. Control plants wilted after 12 days and time to wilting of drenched plants increased with increasing ABA concentrations, up to 23 days in the 1000 ppm treatment. Spray treatments had little effect on gs and no detectable effect on water uptake or time to wilting. Some yellowing of older leaves was seen with ABA drenches of 500 or 1000 ppm. Despite this side effect, ABA drenches have potential to extend the shelf life of hydrangeas in retail environments.

scholarly journals Roles of Glutathione in Mediating Abscisic Acid Signaling and Its Regulation of Seed Dormancy and Drought Tolerance

Genes ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1620
Author(s):  
Murali Krishna Koramutla ◽  
Manisha Negi ◽  
Belay T. Ayele
Keyword(s):  
Signal Transduction ◽  
Abscisic Acid ◽  
Seed Dormancy ◽  
Growth And Development ◽  
Current Knowledge ◽  
Stomatal Closure ◽  
Redox Homeostasis ◽  
Critical Role ◽  
Stress Conditions ◽  
Signal Perception

Plant growth and development and interactions with the environment are regulated by phytohormones and other signaling molecules. During their evolution, plants have developed strategies for efficient signal perception and for the activation of signal transduction cascades to maintain proper growth and development, in particular under adverse environmental conditions. Abscisic acid (ABA) is one of the phytohormones known to regulate plant developmental events and tolerance to environmental stresses. The role of ABA is mediated by both its accumulated level, which is regulated by its biosynthesis and catabolism, and signaling, all of which are influenced by complex regulatory mechanisms. Under stress conditions, plants employ enzymatic and non-enzymatic antioxidant strategies to scavenge excess reactive oxygen species (ROS) and mitigate the negative effects of oxidative stress. Glutathione (GSH) is one of the main antioxidant molecules playing a critical role in plant survival under stress conditions through the detoxification of excess ROS, maintaining cellular redox homeostasis and regulating protein functions. GSH has recently emerged as an important signaling molecule regulating ABA signal transduction and associated developmental events, and response to stressors. This review highlights the current knowledge on the interplay between ABA and GSH in regulating seed dormancy, germination, stomatal closure and tolerance to drought.

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