scholarly journals Anti-inflammatory, analgesic and antioxidant potential of the stem bark of Spondias mangifera Willd.

2011 ◽  
Vol 63 (2) ◽  
pp. 413-419 ◽  
Author(s):  
Nikhil Sachan ◽  
Muhammad Arif ◽  
K. Zaman ◽  
Yatindra Kumar
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Control Group ◽  
Tail Flick ◽  
Alcoholic Extract ◽  
Radical Scavenging Capacity ◽  
Rat Paw Edema ◽  
Significant Prolongation ◽  
Scavenging Capacity ◽  
Anti Inflammatory

The anti-inflammatory and analgesic activities of the ethyl acetate (EAFSM) and n-butanol (NBFSM) fractions of the alcoholic extract of S. mangifera bark were evaluated using carrageenan-induced rat paw edema and by the tail-flick method in rats. The radical scavenging activity of the ethanolic extract, aqueous extract and fractions was determined with the DPPH radical scavenging capacity assay. Two fractions of the alcoholic extract, EAFSM and NBFSM, at doses of 75, 150, 300 mg/kg b.w. administered orally, showed a significant reduction in paw volume when compared with the respective control group challenged by carrageenan. Different doses of extract fractions also showed a significant prolongation of the tail-flick latency of the rat (P<0.01). Different concentrations of alcoholic, aqueous extracts and fractions of alcoholic extract showed significant free radical scavenging capacity against DPPH generated free radicals.

scholarly journals Antioxidant, Anti-Inflammatory and Antithrombotic Effects of Ginsenoside Compound K Enriched Extract Derived from Ginseng Sprouts

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4102
Author(s):  
In-Hee Baik ◽  
Kyung-Hee Kim ◽  
Kyung-Ae Lee
Keyword(s):  
Bleeding Time ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Raw 264.7 Cells ◽  
Control Group ◽  
Compound K ◽  
Antithrombotic Effects ◽  
Macroporous Adsorption Resin ◽  
Anti Inflammatory ◽  
Factor Α

Partially purified ginsenoside extract (PGE) and compound K enriched extract (CKE) were prepared from ginseng sprouts, and their antioxidant, anti-inflammatory and antithrombotic effects were investigated. Compared to the 6-year-old ginseng roots, ginseng sprouts were found to have a higher content of phenolic compounds, saponin and protopanaxadiol-type ginsenoside by about 56%, 36% and 43%, respectively. PGE was prepared using a macroporous adsorption resin, and compound K(CK) was converted and enriched from the PGE by enzymatic hydrolysis with a conversion rate of 75%. PGE showed higher effects than CKE on radical scavenging activity in antioxidant assays. On the other hand, CKE reduced nitric oxide levels more effectively than PGE in RAW 264.7 cells. CKE also reduced pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin (IL)-1β and IL-6 than PGE. Tail bleeding time and volume were investigated after administration of CKE at 70–150 mg/kg/day to mice. CKE administered group showed a significant increase or increased tendency in bleeding time than the control group. Bleeding volume in the CKE group increased than the control group, but not as much as in the aspirin group. In conclusion, ginseng sprouts could be an efficient source of ginsenoside, and CKE converted from the ginsenosides showed antioxidant, anti-inflammatory and antithrombotic effects. However, it was estimated that the CKE might play an essential role in anti-inflammatory effects rather than antioxidant effects.

scholarly journals Constituents of French Marigold (Tagetes patulaL.) Flowers Protect Jurkat T-Cells against Oxidative Stress

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Irakli Chkhikvishvili ◽  
Tamar Sanikidze ◽  
Nunu Gogia ◽  
Maia Enukidze ◽  
Marine Machavariani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
T Cells ◽  
Radical Scavenging ◽  
Jurkat Cells ◽  
Cellular Mechanisms ◽  
Jurkat T Cells ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Inflammatory

The flowers of French marigold (Tagetes patulaL.) are widely used in folk medicine, in particular for treating inflammation-related disorders. However, cellular mechanisms of this activity demand further investigation. In the present work, we studied the potential ofT. patulacompounds to alleviate the oxidative stress in hydrogen peroxide-challenged human lymphoblastoid Jurkat T-cells. Crude extracts of marigold flowers and purified fractions containing flavonoids patuletin, quercetagetin, and quercetin and their derivatives, as well as the carotenoid lutein, were brought in contact with Jurkat cells challenged with 25 or 50 μM H2O2. Hydrogen peroxide caused oxidative stress in the cells, manifested as generation of superoxide and peroxyl radicals, reduced viability, arrested cell cycle, and enhanced apoptosis. The stress was alleviated by marigold ingredients that demonstrated high radical-scavenging capacity and enhanced the activity of antioxidant enzymes involved in neutralization of reactive oxygen species. Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential.T. patulacompounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells. Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

Study on Antioxidant Activity of Aqueous Extracts from Scum of Mixed Juice

2011 ◽  
Vol 396-398 ◽  
pp. 1588-1591
Author(s):  
Yuan Yuan Pu ◽  
Qing Song Zou ◽  
Zi Yan Feng ◽  
An Gen Lu ◽  
Zhong Han ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Water Extraction ◽  
Macroporous Resin ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

The scum of mixed juice is a by-product in sugarcane factory, which used up-floating method for mixed juice clarification. The scum is of great research and utilization value due to its rich organic non-sugar compounds such as proteins, lipids and polyphenols. Studies on the free radical scavenging capacity and reducing power of water extraction sample (A) and macroporous resin purified sample (B) from mixed juice scum were investigated, comparing with Vc. It was showed that the antioxidant activity of macroporous resin purified sample (B) was much stronger than that of water extraction sample (A) and Vc in , and system. For example, the scavenging capacity of sample B on was 11 times than that of Vc and 1450 times of sample A. In addition, the free radical scavenging activity of sample A and B was dependent on the sample concentration. Results in this study indicated that scum of mixed juice may be a promising natural antioxidant, which can be used as additives in food industry or feed industry.

scholarly journals Anti-inflammatory and antinociceptive features of Bryonia alba L.: As a possible alternative in treating rheumatism

2019 ◽  
Vol 17 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Mert Ilhan ◽  
Fatma Tuğçe Gürağaç Dereli ◽  
Ibrahim Tümen ◽  
Esra Küpeli Akkol
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Tail Flick ◽  
Scavenging Activity ◽  
Tail Flick Test ◽  
Etoac Extract ◽  
Anti Inflammatory

AbstractBryonia species have traditionally been used as a diuretic and laxative, to reduce edema and relieve joint pain. B. alba roots are used especially to treat rheumatic pain and applied to painful joints in Turkish folk medicine. B. alba roots were extracted with n-hexane, ethyl acetate (EtOAc), and methanol, successively. The carrageenan-induced hind paw edema model and the Whittle method were used to evaluate the anti-inflammatory activity of the extracts in mice. The p-benzoquinone-induced abdominal constriction test and tail flick test were used to evaluate the antinociceptive activity of the extracts in mice. DPPH-radical-scavenging activity, ABTS radical–scavenging activity, total antioxidant activity, and hydroxyl radical– scavenging activity were assessed. Furthermore, the total phenolic and total flavonoid contents were calculated for all the extracts. The EtOAc extract prepared from B. alba roots had the most promising anti-inflammatory, antinociceptive, and antioxidant activities. Moreover, it had high phenolic and flavonoid contents. Therefore, B. alba roots could be alternative treatment method for rheumatic disorders.

scholarly journals Food Additives In Commercial Cocoa Beverage Products and their Effects on Total Polyphenol Contents, Cellular Antioxidant and Anti-Inflammatory Activities

2021 ◽  
Vol 9 (1) ◽  
pp. 20-30
Author(s):  
Lena Ling ◽  
Claudine Loong ◽  
Wai Mun Loke
Keyword(s):  
Radical Scavenging Activity ◽  
Food Additives ◽  
Radical Scavenging ◽  
Leukotriene B4 ◽  
Food Additive ◽  
Human Neutrophils ◽  
Lipid Hydroperoxides ◽  
Myeloperoxidase Activity ◽  
Scavenging Capacity ◽  
Anti Inflammatory

The study evaluated the uses of food additives in commercial cocoa beverages, and examined the effects of the food additives on their antioxidant and anti-inflammatory activities. The food additive lists of the cocoa beverage items on the shelves and chillers of ten randomly selected local supermarkets were recorded. The total flavonoid, polyphenol contents, and radical scavenging activity of the beverages were determined using the modified Dowd, Folin-Ciocalteu, and 1,1-diphenyl-2-picrylhydrazyl radical scavenging assays, respectively. Cellular experiments examined the inhibition of F2-isoprostanes, lipid hydroperoxides, leukotriene B4 productions, and myeloperoxidase activity by freshly isolated human neutrophils. The effects of food additives on the measured outcomes were evaluated. Food additives were added to 72% of the twenty five cocoa beverage products. Flavorings (60%), antioxidants (56%), pH regulators (40%), emulsifiers (36%), and colorings (4%) were added into these beverages. The cocoa beverages contained significant amounts of flavonoids, polyphenols, and radical-scavenging antioxidants. Their ethanolic extracts inhibited F2-isoprostanes, lipid hydroperoxides, leukotriene B4 productions, and myeloperoxidase activity from freshly isolated human neutrophils. After stratification by different food additive groups, the flavonoids, polyphenols contents, radical scavenging capacity, cellular inhibitions of F2-isoprostanes, lipid hydroperoxides, leukotriene B4, and myeloperoxidase activity were significantly increased by the beverages containing added antioxidants compared to those without. The other additive types did not influence the measured antioxidant and anti-inflammatory outcomes. Commercial cocoa beverages were shown to exert potential nutraceutical properties, such as antioxidant and anti-inflammatory activities. Selective food additives may exert profound effects on these properties by modulating the availability of flavonoids and polyphenols.

scholarly journals Evaluation of anti-nociceptive, anti-inflammatory and hepatoprotective effects of methanol extract of Mazus pumilus (Burm. f.) Steenis (Mazaceae) herb

2021 ◽  
Vol 18 (4) ◽  
pp. 799-807
Author(s):  
Saiqa Ishtiaq ◽  
Ayesha Ilyas ◽  
Numera Irshad ◽  
Uzma Niaz ◽  
Uzma Hanif ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Inflammatory Activity ◽  
Control Group ◽  
Tail Flick ◽  
Histopathological Analysis ◽  
Standard Drug ◽  
Rat Paw Edema ◽  
Hepatoprotective Effects ◽  
Anti Inflammatory ◽  
Different Doses

Purpose: This study was designed to investigate the anti-nociceptive, anti-inflammatory and hepatoprotective activities of the methanol extract of Mazus pumilus (Mazaceae) herb. Methods: Anti-nociceptive activity was determined using hot plate, tail flick and acetic acid-induced writing methods. Carrageenan-induced rat paw edema (0.1 mL of 1 %) model was used for the assessment of anti-inflammatory activity. The methanol extract was administered orally at three different doses (150, 300 and 600 mg/kg) to three separate groups in all the experiments. Diclofenac sodium (50 mg/kg) was used as standard drug while control group received DMSO (1 %, 10 mL/kg). The hepatocurative effect of methanol extract of M. pumilus (400 mg/kg) was determined in isoniazid (50 mg/kg) and rifampicin (100 mg/kg) induced liver injury. Silymarin (100 mg/kg) was used as standard drug for comparison. The control group received distilled water (10 mL/kg). Preliminary phytochemical screening was also carried out. Results: The methanol extract of M. pumilus significantly (p < 0.05) augmented latency time and reduced the number of writhes in the pain models at all doses used for the assessment of antinociceptive actions. The anti-inflammatory activity of different doses of extract was evaluated by measuring the reduction in the size of the paw. A significant (p < 0.05) hepatocurative effect was observed when administered after anti-tuberculosis drugs. Histopathological analysis of the liver tissues also revealed restored hepatocellular architecture. Conclusion: The results demonstrate the anti-nociceptive, anti-inflammatory and hepatoprotective effects of the methanol extract of M. pumilus, thus substantiating the ethnomedical claims associated with the herb.

scholarly journals Characteristics and Free Radical Scavenging Activity of Zinc Oxide (ZnO) Nanoparticles Derived from Extract of Coriander (Coriandrum sativum L.)

REAKTOR ◽  
2017 ◽  
Vol 17 (3) ◽  
pp. 145 ◽  
Author(s):  
Tagor Marsillam Siregar ◽  
Antonius Herry Cahyana ◽  
Regina Jessilia Gunawan
Keyword(s):  
Free Radical ◽  
Zno Nanoparticles ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Coriandrum Sativum ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

Abstract Zinc Oxide (ZnO) nanoparticles were recently studied for its free radical scavenging activity.                     The formation of ZnO nanoparticles by means of biological method was known to be a simpler and more environmental friendly method. One of the biological methods used for the formation of ZnO nanoparticles was by utilizing the phytochemicals that were present in a plant extract.                                       The phytochemicals such as phenolic and flavonoid compound was able to reduce bulk metal Zinc into Zinc nanoparticles. Coriander (Coriandrum sativum L.) seeds and leaves were known to contain high amounts of phenolic compounds and flavonoid compounds. The aim of this study was to investigate the effect of different solvent polarities used to extract Coriander leaves and seeds towards the reducing power of plant extract and the free radical scavenging capacity of ZnO nanoparticles. The different solvents that were used to extract Coriander were deionized water, ethyl acetate, and hexane. The different solvent polarities affected the weight of the derived ZnO nanoparticles due to the different types of the extracted phenolic and flavonoid compounds. The different solvent polarities also affected the free radical scavenging capacity of ZnO nanoparticles. Ethyl acetate based extract of Coriander leaf resulted in the most weight of ZnO nanoparticles, 0.7907 g and the highest free radical scavenging capacity, 11.16%/mg ZnO nanoparticles. The free radical scavenging activity of ZnO nanoparticles decreased to 3.67%/mg ZnO nanoparticles after the process of calcination at 100°C and decreases to 0 %/mg ZnO nanoparticles after the process of calcination at 300 and 500°C. The diameter of ZnO particles increases from 128.1±33.5 nm to 552.3±61.1 nm after calcination. Kata kunci: calcination; free radical scavenging capacity; polarities; zinc oxide nanoparticles  Abstrak KARAKTERISTIK DAN AKTIVITAS PENANGKAL RADIKAL BEBAS NANOPARTIKEL SENG OKSIDA (ZnO) YANG DIBENTUK MENGGUNAKAN EKSTRAK KETUMBAR (Coriandrum sativum L.). Nanopartikel Seng Oksida (ZnO) saat ini tengah diteliti sehubungan dengan kapasitasnya dalam menangkal radikal bebas. Metode biologis merupakan metode sederhana dan ramah lingkungan yang dapat digunakan pada pembentukan nanopartikel ZnO. Pemanfaatan senyawa fitokimia dari ekstrak tanaman merupakan salah satu metode biologis yang dapat digunakan pada pembentukan nanopartikel ZnO. Senyawa fitokimia seperti senyawa fenolik dan flavonoid dapat mereduksi logam Seng menjadi nanopartikel Seng. Biji dan daun dari tanaman ketumbar (Coriandrum sativum L.) telah diketahui memiliki kandungan senyawa fenolik dan flavonoid. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh perbedaan polaritas pelarut yang digunakan untuk ekstraksi biji dan daun ketumbar terhadap kapasitas reduksi ekstrak dan penangkal radikal bebas dari nanopartikel ZnO. Pelarut yang digunakan pada ekstraksi biji dan daun ketumbar adalah air deionisasi, etil asetat dan heksana. Perbedaan polaritas pelarut mempengaruhi berat nanopartikel ZnO yang diperoleh, hal ini disebabkan oleh perbedaan jenis senyawa fenolik dan flavonoid yang terekstrak. Perbedaan polaritas pelarut juga mempengaruhi kapasitas penangkal radikal bebas  dari nanopartikel ZnO. Ekstrak etil asetat daun ketumbar menghasilkan nanopartikel ZnO dengan berat 0,7907 g   dan aktivitas penangkal radikal bebas sebesar 11,16%/mg nanopartikel ZnO. Aktivitas penangkal radikal bebas nanopartikel ZnO menurun menjadi 3,67%/mg nanopartikel ZnO setelah dikalsinasi pada suhu 100oC dan menurun menjadi 0%/mg nanopartikel ZnO setelah dikalsinasi pada suhu 300oC, 500oC. Diameter nanopartikel ZnO meningkat dari 128,1±33,5 nm menjadi  552,3±61,1 nm setelah dikalsinasi Keywords: elektrokoagulasi, penurunan warna, penurunan TSS, laju degradasi COD, imbah tekstil

scholarly journals Extraction Process, Component Analysis, and In Vitro Antioxidant, Antibacterial, and Anti-Inflammatory Activities of Total Flavonoid Extracts from Abutilon theophrasti Medic. Leaves

2018 ◽  
Vol 2018 ◽  
pp. 1-17 ◽  
Author(s):  
Chunlian Tian ◽  
Peng Zhang ◽  
Caixia Yang ◽  
Xiang Gao ◽  
Hong Wang ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Extraction Process ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Inflammatory ◽  
Flavonoid Fraction ◽  
Process Component

The flavonoid fraction was extracted from the leaves of Abutilon theophrasti Medic., which are usually used as a traditional Chinese herbal medicine for the treatment of inflammation and joint pain. The current study focused on the extraction process, component analysis, and in vitro antioxidant, antibacterial, and anti-inflammatory activities of the flavonoid fraction as a part of ongoing research on bioactive substances from natural plant sources. This study evaluated the antioxidant activities via assays of DPPH radical scavenging capacity, ABTS radical scavenging capacity, and reducing power and investigated inhibitory activities against Escherichia coli, Salmonella, Staphylococcus aureus, and Streptococcus. Moreover, the inflammatory activity of the flavonoid fraction was estimated by measurement of the content of tumor necrosis factor alpha, interleukin-1-beta, interleukin-6, interleukin-10, nitric oxide, and cyclooxygenase-2 and the gene expression levels of several inflammation markers, such as inducible nitric oxide synthase and cyclooxygenase-2, in RAW 264.7 macrophages after LPS treatment. In addition, the underlying anti-inflammatory mechanisms, that is, the activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, were also revealed from the gene and protein expression levels. Taken together, these results suggested that the flavonoid fraction might exert in vitro antioxidant, antibacterial, and anti-inflammatory effects on LPS-stimulated RAW 264.7 macrophages and will be potentially useful as an adjuvant treatment for oxidative stress and bacterial and inflammatory diseases.

scholarly journals Investigating the Antioxidant Characteristics of Saccharina Latissima and Its Ability to Reduce Inflammation in RAW 264.7 Cells (P06-091-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Abigail Wiegand ◽  
Gretchen Grebe ◽  
Carrie Byron ◽  
Angela Myracle
Keyword(s):  
Gallic Acid ◽  
Radical Scavenging ◽  
Saccharina Latissima ◽  
Total Phenolic ◽  
Raw 264.7 ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Inflammatory ◽  
The University ◽  
Gallic Acid Equivalent

Abstract Objectives The goal of this study is to understand the anti-inflammatory characteristics of Saccharina latissima (sugar kelp). Sugar kelp is a brown macroalga that grows readily off the coast of Maine. Other seaweeds are known to contain bioactive chemicals that reduce inflammation—a contributing factor to metabolic syndrome and type 2 diabetes. It is hypothesized that injuring farmed sugar kelp by trimming the kelp may increase the beneficial bioactives and promote anti-inflammatory effects. The specific aims are (1) to produce extracts of Maine-grown sugar kelp through accelerated solvent extraction (ASE), (2) to measure the samples’ antioxidant capacity, and (3) to investigate the samples’ anti-inflammatory effect in vitro. Methods Sugar kelp samples were freeze-dried and extracts were produced using ASE. Total phenolic content was measured using the Folin-Ciocalteau assay, and radical scavenging capacity was measured with a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The sugar kelp extracts were tested for cytotoxicity in RAW 264.7 macrophages using a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Lipopolysaccharide (LPS) was used to induce inflammation in the cells, and the attenuation effect of extract doses ranging from 12.5–50 µg total phenolics (TP)/mL was observed by measuring nitric oxide (NO) secretion with the Griess assay. Results Preliminary data shows that sugar kelp contains polyphenols (8.4–16.0 mg gallic acid equivalent/g dried biomass) and radical scavenging capacity (IC50 = 61.8–79.5 µg/mL). The data did not demonstrate the trimming the kelp has a beneficial effect to increase phenolic compounds. The polyphenolic contents of the trimmed samples were lower than the untrimmed (9.5 vs. 13.1 mg gallic acid equivalent/g dried biomass). The radical scavenging capacity decreased when the samples were trimmed (14.7%). The greatest decrease in NO production was at a concentration of 50 μg TP/mL for a trimmed sample (up to 78.5%), which supports the hypothesis. Conclusions This data suggests that sugar kelp may be a good source of antioxidants and trimmed samples may contain additional bioactives to help decrease inflammation. Further investigation is needed to confirm the hypothesis. Funding Sources The University of Maine The University of New England

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