Continuous ethanol production from sugar beet thick juice by Saccharomyces cerevisiae immobilized onto sugar beet pulp

The immobilization of Saccharomyces cerevisiae onto sugar beet pulp (SBP) by natural adhesion is an efficient and low-cost method for retaining high biocatalyst density in the ethanol fermentation system. In the present study, cells of S. cerevisiae 163, were immobilized by natural adhesion onto SBP. The retention of immobilized cells attained the level of about 1.7?1011 cells/gram of dry SBP. Continuous ethanol production from sugar beet thick juice (TJ) was performed in a cylinder glass bioreactor at a temperature of 30?C and pH 5 during a 27-day period. The stability of the fermentation process at dilution rate (D) of 0.025 h-1 and 0.05 h-1 was evaluated. The yeast-SBP system was shown to be stable for over a 15-day period at the dilution rate of 0.025 h-1, while the dilution rate of 0.05 h-1 was found to be unsuitable due to the intensive yeast leaching from the support. At D of 0.025 h-1 the maximum sugar utilization (Su), ethanol concentration (P), volumetric ethanol productivity (Qp), ethanol yield (Yp/s) and fermentation efficiency were 97.1%, 54.7 g/l, 2.3 g/lh, 0.498 g/g and 97.6%, respectively.

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Ethanol Production from Sugar Beet Pulp using Escherichia coli KO11 and Saccharomyces cerevisiae

Biological Engineering ◽

10.13031/2013.37124 ◽

2011 ◽

Vol 3 (4) ◽

pp. 199-209 ◽

Cited By ~ 3

Author(s):

R. Rorick ◽

N. Nahar ◽

S. W. Pryor

Keyword(s):

Escherichia Coli ◽

Saccharomyces Cerevisiae ◽

Sugar Beet ◽

Ethanol Production ◽

Sugar Beet Pulp ◽

Escherichia Coli Ko11 ◽

Beet Pulp

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A low-cost method for obtaining high-value bio-based propylene glycol from sugar beet pulp

RSC Advances ◽

10.1039/c4ra12839g ◽

2015 ◽

Vol 5 (3) ◽

pp. 2299-2304 ◽

Cited By ~ 13

Author(s):

J. Berlowska ◽

M. Binczarski ◽

M. Dudkiewicz ◽

H. Kalinowska ◽

I. A. Witonska ◽

...

Keyword(s):

Sugar Beet ◽

Propylene Glycol ◽

Low Cost ◽

Sugar Beet Pulp ◽

Beet Pulp

A new low-cost pathway for the production of high-value propylene glycol (PG) is proposed.

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Sugar beet pulp as biomass

Sugar Industry ◽

10.36961/si18105 ◽

2017 ◽

pp. 29-32 ◽

Cited By ~ 1

Author(s):

Kazm Eber Özba ◽

Özen Özboy Özba

Keyword(s):

Sugar Beet ◽

Animal Feed ◽

Sugar Industry ◽

Low Cost ◽

Industrial Applications ◽

Single Cell Protein ◽

Sugar Beet Pulp ◽

Raw Material ◽

Cell Protein ◽

Beet Pulp

The sugar beet industry produces considerable amounts of organic waste and by-products. Sugar beet pulp (SBP) is the residue that remains after sugar extraction. SBP is a lignocellulosic by-product of the sugar industry and generally used as animal feed at relatively low price. Instead of cattle feeding, SBP can be used as a raw material for industrial applications because it is low-cost and available in large amounts. Biomass is a clean and renewable energy source. The use of SBP for the production of ethanol, methanol, single cell protein, biofuels etc. is economically very attractive. This literature review evaluates the use of SBP as biomass.

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Simultaneous Saccharification and Fermentation of Sugar Beet Pulp for Efficient Bioethanol Production

BioMed Research International ◽

10.1155/2016/3154929 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 24

Author(s):

Joanna Berłowska ◽

Katarzyna Pielech-Przybylska ◽

Maria Balcerek ◽

Urszula Dziekońska-Kubczak ◽

Piotr Patelski ◽

...

Keyword(s):

Sugar Beet ◽

Dry Matter ◽

Simultaneous Saccharification And Fermentation ◽

Ethanol Yield ◽

Pichia Stipitis ◽

Sulphuric Acid Solution ◽

Sugar Beet Pulp ◽

Enzyme Preparations ◽

Beet Pulp ◽

Simultaneous Saccharification

Sugar beet pulp, a byproduct of sugar beet processing, can be used as a feedstock in second-generation ethanol production. The objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release of fermentable sugars and ethanol yield during simultaneous saccharification and fermentation (SSF) of sugar beet pulp-based worts. Pressure-thermal pretreatment was applied to sugar beet pulp suspended in 2% w/w sulphuric acid solution at a ratio providing 12% dry matter. Enzymatic hydrolysis was conducted using Viscozyme and Ultraflo Max (Novozymes) enzyme preparations (0.015–0.02 mL/g dry matter). Two yeast strains were used for fermentation: Ethanol Red (S. cerevisiae) (1 g/L) andPichia stipitis(0.5 g/L), applied sequentially. The results show that efficient simultaneous saccharification and fermentation of sugar beet pulp was achieved. A 6 h interval for enzymatic activation between the application of enzyme preparations and inoculation with Ethanol Red further improved the fermentation performance, with the highest ethanol concentration reaching26.9±1.2 g/L and86.5±2.1%fermentation efficiency relative to the theoretical yield.

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Ethanol fermentation of molasses by Saccharomyces cerevisiae cells immobilized onto sugar beet pulp

Acta periodica technologica ◽

10.2298/apt1243325v ◽

2012 ◽

pp. 325-333 ◽

Cited By ~ 6

Author(s):

Vesna Vucurovic ◽

Radojka Razmovski

Keyword(s):

Saccharomyces Cerevisiae ◽

Sugar Beet ◽

Glucose Concentration ◽

Ethanol Fermentation ◽

Dry Mass ◽

Sugar Beet Pulp ◽

Yeast Cells ◽

Inoculum Concentration ◽

Beet Molasses ◽

Beet Pulp

Natural adhesion of Saccharomyces cerevisiae onto sugar beet pulp (SBP) is a very simple and cheap immobilization method for retaining high cells density in the ethanol fermentation system. In the present study, yeast cells were immobilized by adhesion onto SBP suspended in the synthetic culture media under different conditions such as: glucose concentration (100, 120 and 150 g/l), inoculum concentration (5, 10 and 15 g/l dry mass) and temperature (25, 30, 35 and 40?C). In order to estimate the optimal immobilization conditions the yeast cells retention (R), after each immobilization experiment was analyzed. The highest R value of 0.486 g dry mass yeast /g dry mass SBP was obtained at 30?C, glucose concentration of 150 g/l, and inoculum concentration of 15 g/l. The yeast immobilized under these conditions was used for ethanol fermentation of sugar beet molasses containing 150.2 g/l of reducing sugar. Efficient ethanol fermentation (ethanol concentration of 70.57 g/l, fermentation efficiency 93.98%) of sugar beet molasses was achieved using S. cerevisiae immobilized by natural adhesion on SBP.

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Removal of acridine orange dye from aqueous solution by adsorption onto dried sugar beet pulp

Acta periodica technologica ◽

10.2298/apt1748307v ◽

2017 ◽

pp. 307-314

Author(s):

Vesna Vucurovic ◽

Vladimir Puskas ◽

Uros Miljic

Keyword(s):

Sugar Beet ◽

Acridine Orange ◽

Low Cost ◽

Sugar Beet Pulp ◽

Isotherm Models ◽

Maximum Adsorption Capacity ◽

Solution Ph ◽

Equilibrium Data ◽

Beet Pulp ◽

Pseudo Second Order

A simple, low cost, and effective method for the removal of acridine orange (AO), a mutagenic cationic dye, from aqueous model solutions by adsorption onto dried sugar beet pulp (SBP) was evaluated in the present study. The AO removal was enhanced along with the increase of the initial solution pH and dye concentration. It was found that the adsorption process closely follows a pseudo-second-order chemisorption kinetics. The obtained equilibrium data obey both the Freundlich and Langmuir isotherm models. The SBP was proved to be very promising adsorbent for AO removal. Maximum adsorption capacity of the Langmuir monolayer of SBP for AO was found to be 5.37, 34.6, 89.62, 144.53 and 324.58 mg/g, at 25?C for the solution pH of 2, 4, 5, 6, and 8, respectively.

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