scholarly journals Microbiological quality of fresh vegetable salads from the Novi Sad market

2018 ◽  
pp. 21-30
Author(s):  
Dragoljub Cvetkovic ◽  
Dragan Pavlovic ◽  
Aleksandra Ranitovic ◽  
Sinisa Markov
Keyword(s):  
Listeria Monocytogenes ◽  
Microbiological Quality ◽  
Aerobic Bacteria ◽  
Salmonella Spp ◽  
Fresh Vegetable ◽  
Efficient Delivery ◽  
Microbiological Methods ◽  
Fresh Vegetables ◽  
Yeasts And Molds ◽  
Novi Sad

Increased consumption, big production units and very efficient delivery service networks of fresh vegetables and salads caused a higher number of foodborne illnesses in the last two decades all over the world. In this study 15 fresh vegetable salads from different marketplaces in Novi Sad were tested by reference microbiological methods (detection of Salmonella spp., enumeration of Listeria monocytogenes, mesophilic aerobic bacteria, yeasts and molds, sporogenic mesophilic aerobic bacteria, sporogenic sulfite-reducing bacteria, Bacillus cereus, Enterobacteriaceae, Escherichia coli and coagulase positive staphylococci). Salmonella spp. was found in one sample which, according to the safety criteria for such a product (if treated as ready-to-eat), was considered to be microbiologically unsafe. The number of Listeria monocytogenes in all tested samples was <100 cfu/g, which is a satisfactory result according to the safety criteria. E. coli was detected in only one sample, and the number of Enterobacteriaceae was in the range from 4.4 to 6.9 log CFU/g. The isolated Enterobacteriaceae strains were identified as Enterobacter cloacae, Klebsiella pneumoniae ssp. pneumoniae and Citrobacter spp.

Efficacy of Chlorine Dioxide Gas Sachets for Enhancing the Microbiological Quality and Safety of Blueberries

2007 ◽  
Vol 70 (9) ◽  
pp. 2084-2088 ◽  
Author(s):  
IULIANO POPA ◽  
ERIC J. HANSON ◽  
EWEN C. D. TODD ◽  
ANNEMIEK C. SCHILDER ◽  
ELLIOT T. RYSER
Keyword(s):  
Chlorine Dioxide ◽  
Effective Means ◽  
Microbiological Quality ◽  
Microbial Inactivation ◽  
Aerobic Bacteria ◽  
Salmonella Spp ◽  
E Coli ◽  
Chlorine Dioxide Gas ◽  
Standard Procedures ◽  
Yeasts And Molds

In response to increasingly stringent microbial specifications being imposed by purchasers of frozen blueberries, chlorine dioxide (ClO2) gas generated by a dry chemical sachet was assessed for inactivation of Listeria monocytogenes, Salmonella spp., and Escherichia coli O157:H7 as well as five yeasts and molds known for blueberry spoilage. Fresh blueberry samples (100 g) were separately inoculated with cocktails of L. monocytogenes, Salmonella, E. coli O157:H7 (three strains each), or yeasts and molds (five strains each) to contain ∼106 CFU/g and exposed to ClO2 (4 mg/liter, 0.16 mg/g) for 12 h in a sealed 20-liter container (99.9% relative humidity) at ∼22°C. After gassing, 25 g of blueberries was added to 225 ml of neutralizing buffer, pulsified for 1 min, and plated using standard procedures to quantify survivors. This treatment yielded reductions of 3.94, 3.62, 4.25, 3.10, and 3.17 log CFU/g for L. monocytogenes, Salmonella, E. coli O157:H7, yeasts, and molds, respectively. Thereafter, 30 lugs of uninoculated blueberries (∼9.1 kg per lug) were stacked on 1.2 by 1.2-m pallets (5 lugs per level × six levels), tarped, and exposed to ClO2 (18 mg/liter, 0.13 mg/g) for 12 h. After gassing, significant (P &lt; 0.05) reductions of 2.33, 1.47, 0.52, 1.63, and 0.48 log CFU/g were seen for mesophilic aerobic bacteria, coliforms, E. coli, yeasts, and molds, respectively, compared with non-gassed controls. No significant differences (P &gt; 0.05) in microbial inactivation were seen between lug levels and, with one exception (mesophilic aerobic bacteria), between the bottom and top surface of individual lugs. Based on these findings, ClO2 sachets may provide a simple, economical, and effective means of enhancing the microbial shelf life and safety of blueberries.

scholarly journals Tokat Bez Sucuğunun Mikrobiyolojik Kalitesinin İncelenmesi

2020 ◽  
Vol 8 (12) ◽  
pp. 2683-2694
Author(s):  
Nesrin Kaval ◽  
Nilgün Öncül ◽  
Zeliha Yıldırım
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Fecal Coliform ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Aerobic Bacteria ◽  
Escherichia Coli O157 ◽  
Salmonella Spp ◽  
Microbiological Parameters

In this study, the microbiological quality of traditionally produced Bez Sucuk in Tokat and its vicinity was investigated. For this purpose, 30 Bez Sucuk samples obtained from butchers, producers of Bez Sucuk, were analyzed for total count of mesophilic aerobic bacteria, lactic acid bacteria, yeasts-moulds, total coliform, fecal coliform, Staphylococcus aureus, Bacillus cereus, and Cl. perfringens. Also, the presence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. were investigated in all samples. pH and water activity of the samples were determined. As the result of the analysis, the counts of the microbiological parameters investigated were found as follows: total mesophilic aerobic bacteria 3.5×106-4.23×109 CFU/g, lactic acid bacteria 5.55×105-2.45×109 CFU/g, yeasts and moulds 2.50×103-6.90×109 CFU/g, total coliform

Microbiological Quality of Retail Imported Unprepared Whole Lettuces: A PHLS Food Working Group Study

1999 ◽  
Vol 62 (4) ◽  
pp. 325-328 ◽  
Author(s):  
C. LITTLE ◽  
D. ROBERTS ◽  
E. YOUNGS ◽  
J. de LOUVOIS
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Microbiological Quality ◽  
Escherichia Coli O157 ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Natural Microflora ◽  
Production Practices ◽  
Campylobacter Spp

A study of imported unprepared whole lettuces sampled from supermarkets, greengrocers, shops, and market stalls found that all were of acceptable microbiological quality. Twenty-seven out of 151 (18%) imported lettuce samples had Enterobacteriaceae levels of 104 CFU/g or more. However, these bacteria that constitute part of the natural microflora of unprepared vegetables may also be derived from the soil and/or by poor handling. The pathogens, Salmonella spp., Shigella spp., Campylobacter spp., Escherichia coli O157:H7, Vibrio cholerae, Listeria monocytogenes, and also Escherichia coli, an indicator of fecal contamination, were not detected in any imported lettuces, indicating that hygiene, harvesting, and production practices were good. Imported lettuces with Enterobacteriaceae levels of 104 CFU/g or more varied with type of retail premises and the temperature at which the lettuces were displayed. Samples from greengrocers, shops, and market stalls were more likely to contain Enterobacteriaceae at levels in excess of 104 CFU/g than those from supermarkets.

Microbial Quality, Safety, and Pathogen Detection by Using Quantitative PCR of Raw Salad Vegetables Sold in Dhanbad City, India

2016 ◽  
Vol 80 (1) ◽  
pp. 121-126 ◽  
Author(s):  
SUJEET K. MRITUNJAY ◽  
VIPIN KUMAR
Keyword(s):  
Food Safety ◽  
Quantitative Pcr ◽  
Indian Subcontinent ◽  
Microbiological Quality ◽  
Plate Count ◽  
Salmonella Spp ◽  
Total Coliforms ◽  
E Coli ◽  
Fresh Vegetables ◽  
Salad Vegetables

ABSTRACT Consumption of ready-to-eat fresh vegetables has increased worldwide, with a consequent increase in outbreaks caused by foodborne pathogens. In the Indian subcontinent, raw fresh vegetables are usually consumed without washing or other decontamination procedures, thereby leading to new food safety threats. In this study, the microbiological quality and pathogenic profile of raw salad vegetables was evaluated through standard protocols. In total, 480 samples (60 each of eight different salad vegetables) of cucumber, tomato, carrot, coriander, cabbage, beetroot, radish, and spinach were collected from different locations in Dhanbad, a city famous for its coal fields and often called the “Coal Capital of India.” The samples were analyzed for total plate count, total coliforms, Escherichia coli, E. coli O157:H7, Listeria monocytogenes, and Salmonella spp. Incidences of pathogens were detected through quantitative PCR subsequent to isolation. Results showed that 46.7% (for total plate counts) and 30% (for total coliforms) of samples were unacceptable for consumption per the Food Safety and Standards Authority of India. Pathogenic microorganisms were detected in 3.7% of total samples. E. coli O157:H7 was detected in three samples of spinach (2) and beetroot (1); L. monocytogenes was detected in 14 samples of spinach (8), tomato (3), cucumber (2), and radish (1); and Salmonella spp. were detected in 16 samples of spinach (7), tomato (3), beetroot (2), cucumber (2), carrot (1), and radish (1). Pathogens were not detected in any of the cabbage and coriander samples.

scholarly journals Microbiological Quality Assessment of Popular Fresh Date Samples Available in Local Outlets of Dhaka City, Bangladesh

2018 ◽  
Vol 2018 ◽  
pp. 1-4
Author(s):  
Rausan Zamir ◽  
A. B. M. Nazmul Islam ◽  
Anisur Rahman ◽  
Sunzid Ahmed ◽  
M. Omar Faruque
Keyword(s):  
Food Poisoning ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Aerobic Bacteria ◽  
Dhaka City ◽  
Hygiene Practices ◽  
Yeasts And Molds ◽  
Potential Human Health ◽  
Date Fruits

It is evident that date fruits provide a wide variety of essential nutrients which impart potential human health benefits. In Bangladesh, the popularity of date and its consumption surge few-fold during Ramadan among Muslims owing to the profound emotion related to religious belief that breaking of dawn-to-dusk fasting with dates is fulfilling a Sunnah. The present study aimed to explore the microbiological quality of the five most popular fresh date samples (Nagal, Dhapas, Boroi, Morium, and Tunisia) purchased from different locations of Dhaka City, Bangladesh. Among 25 samples tested, 23 were observed to carry 3.30-5.65 Log CFU/gm aerobic bacteria and 3.30-5.36 Log CFU/gm yeasts and molds population. Coliform bacteria and Escherichia coli were not found in any of the samples analyzed in this study. However, except Nagal and Boroi from Mohammadpur and Mirpur, respectively, none of the samples were found safe to consume according to the microbiological grade recommended by Woolworths Quality Assurance Standard (WQAS), 2009, as fresh dates with microbial load can cause food poisoning or even foodborne intoxication. Date samples with less or no processing is responsible for this contamination which can be subsided and eliminated by appropriate handling and hygiene practices during postharvest processing.

scholarly journals Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk

2019 ◽  
Vol 24 (1) ◽  
pp. 277-294
Author(s):  
Rocio Esperanza Patiño-Burbano ◽  
Ana Karina Carrascal ◽  
Jorge Luis Parra-Arango ◽  
José Luis Rodríguez-Bautista
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Detection Rate ◽  
Pathogenic Bacteria ◽  
Simultaneous Detection ◽  
Cow Milk ◽  
Escherichia Coli O157 ◽  
Salmonella Spp ◽  
E Coli ◽  
Microbiological Methods

Raw cow milk is considered one of the most important vehicles for pathogenic bacteria like Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes. These three bacteria are responsible for foodborne diseases. Routine microbiological methods to detect these microorganisms in cow milk can be complicated and time consuming. The aim of this work was to evaluate a method to simultaneously detect Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes in experimentally contaminated cow milk. The assessed method combined a standard microbiological culture step, using a pre-enrichment medium that favors the growth of the three focal microorganisms: SEL broth, followed by a single PCR assay. A total of 43 interference bacterial strains were used to evaluate the method’s specificity. The detection rate for the microbiological method with standard culture media was 10 UFC/mL, and that of the PCR detection, following pre-enrichment in SEL broth, was 10 UFC/mL for S. enterica and L. monocytogenes and between 1 and 5 UFC/mL for E. coli O157:H7. The PCR method showed specificity for the reference strains. Simultaneous detection by multiple PCR using SEL broth was successful for the detection of S. enterica, E. coli O157:H7, and L. monocytogenes in samples of experimentally contaminated cow milk, featuring both a high detection rate and a high specificity. This approach promises to be a feasible routine procedure when testing milk samples in industry and public health control setups.

scholarly journals Prevalence of Listeria spp. with PRS Genes in Fresh Vegetables from Traditional Markets

Biomedika ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 123-129
Author(s):  
A. Kaisar Adiwijaya Putra ◽  
Rizalinda Sjahril ◽  
Arif Santoso ◽  
Dianawaty Amiruddin ◽  
Andi Alfian Zainuddin ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Listeria Monocytogenes ◽  
Mortality Rate ◽  
Additional Data ◽  
Traditional Markets ◽  
Vegetable Samples ◽  
Fresh Vegetable ◽  
Listeria Species ◽  
Fresh Vegetables ◽  
Phenotype Analysis

Listeriosis is a foodborne infectious disease caused by Listeria monocytogenes and is considered a serious health problem, due to the severity of symptoms and a high mortality rate in worldwide This study aims to identify and determine the prevalence of Listeria species through prs gene screening of fresh vegetables distributed in several markets of Makassar City. A total of 57 fresh vegetable samples were collected from February to May 2019 in four traditional markets Makassar. The isolates is examines to phenotypically and genotypically Vitek and Multiplex PCR with prs and lmo1030 primer. Phenotype analysis did not show the presence of Listeria species, but the results of genotypic was found 8 positive prs gene samples (14.03%), consisting of 31.2% long beans, 18.2% cabbage, and 9.1% cucumber and. All Listeria species found in this study is Listeria monocytogenes. This study also provide information and additional data that prs genes can be used as screening genotype for identify Listeria species in fresh vegetables.

scholarly journals Sivas kofte and examination of microbiological quality

2013 ◽  
Vol 29 (1) ◽  
pp. 133-143 ◽  
Author(s):  
O.P. Can ◽  
S. Şahin ◽  
M. Erşan ◽  
F. Harun
Keyword(s):  
Meat Product ◽  
Microbiological Quality ◽  
Aerobic Bacteria ◽  
Psychrophilic Bacteria ◽  
Salmonella Spp ◽  
E Coli ◽  
Microbiological Criteria

The objective of this study was to examine traditional meat product of the Sivas province, the Sivas kofte with regards to its microbiological quality. The kofte samples sold commercially were examined according to their microbiological qualities (150 pieces cooked kofte samples taken from the most popular 5 restaurants). The samples were analyzed in terms of total mesophilic aerobic bacteria, Enterobacteria, E. coli, coagulase positive S. aureus, Salmonella spp. and psychrophilic bacteria. Ready to serve samples of Sivas kofte were examined and the following results were obtained for total mesophilic aerobic bacteria, Enterobacteria, coagulase positive S. aureus, psychrophilic bacteria, 2.7- 4.9 log10 cfu/g, <10 - 2.1 log10 cfu /g, <10 - 1.9 log10 cfu /g, 1.6- 3.8 log10 cfu /g, respectively. E. coli and Salmonella spp were not determined in any of the samples. As a result, the ready to consume Sivas kofte samples were found to be in accordance with the Turkish Food Codex Cominiquate Microbiological Criteria despite differences in the microbiological quality of the locations in Sivas.

scholarly journals Influencia de las BPM sobre la calidad microbiológica del queso amasado en las pymes de la provincia del Carchi, Ecuador

2020 ◽  
Vol 5 (2) ◽  
pp. 1
Author(s):  
Wilman Jenny Yambay Vallejo ◽  
Miguel Ángel Anchundia ◽  
Carlos Paredes ◽  
Marcelo Benavides
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Microbiological Quality ◽  
Professional Staff ◽  
Processing Plant ◽  
Legal Regulations ◽  
Salmonella Spp ◽  
Good Manufacturing Practices ◽  
Processing Plants ◽  
Buenas Prácticas

  Con la finalidad de determinar la influencia de las buenas prácticas de manufactura (BPM) sobre la calidad microbiológica de los quesos amasados producidos por nueve fábricas de la Provincia del Carchi, se analizó mohos, levaduras, Escherichia coli, Salmonella spp. Listeria monocytogenes, según la metodología oficial. Los datos obtenidos se analizaron mediante la aplicación de estadística descriptiva, comparando los resultados con valores de referencia para algunas de las determinaciones, los establecidos en la Norma NTE INEN 1528:2012. En lo que respecta a Escherichia coli la planta de procesamiento PP1 y PP3 tuvieron conteo de 1,0x101 y 4,0x101 UFC/g respectivamente, incumpliendo con la normativa nacional vigente. Con referencia a Salmonella spp y Listeria monocytogenes estuvieron ausentes en todas las muestras estudiadas, para los indicadores de calidad mohos, se encontró menor a 10, mientras que para levaduras la PP1 y PP3 fueron menores a 10, las otras plantas de procesamiento tuvieron un contaje de 1,0x102 y 4,6x102 UFC/g correspondiendo el valor más alto al establecimiento de procesamiento identificado como PP4, también se evidenció que el 55,6% de las empresas no realiza control de calidad de la materia prima y el 11,1% de plantas no pasteuriza la leche. Las diferencias encontradas sugieren la no aplicación de buenas prácticas de manufactura en los procesos productivos y la carencia de personal profesional en el 89,9% de las plantas procesadoras, lo cual conlleva al no cumplimiento de las normativas legales referente a los quesos frescos (Normas INEN 1528 y 3067).   Palabra clave: Calidad microbiológica, Buenas Prácticas de Manufactura, quesos frescos, normativas legales.   Abstract In order to determine the influence of the GMP on the microbiological quality of the kneading cheeses produced by nine factories of the Province of Carchi, molds, yeasts, Escherichia coli, Salmonella spp. Listeria monocytogenes were determined by according to the official methodology. The data obtained were analyzed through the descriptive statistics, comparing the results with reference values for some of the determinations, those established in the INEN 1528 Standard, (2012). With respect to Escherichia coli, the processing plant PP1 and PP3 had a count of 1.0x101 and 4.0x101 CFU/g, respectively, no complying with current national regulations. With reference to Salmonella spp and Listeria monocytogenes were absent in all samples studied, for mold quality indicators, were found in a count less than 10, while for yeast PP1 and PP3 had a count less than 10, the other processing plants had a count of 1.0x102 and 4.6x102 CFU/g corresponding the highest value to the processing establishment identified as PP4, it was also shown that 55.6% of the companies do not perform quality control of the raw material and 11.1% of plants do not pasteurize milk. The differences obtained suggest the non-application of Good Manufacturing Practices in the productive processes and the lack of professional staff in 89.9% of the processing plants, which leads to non-compliance with the legal regulations regarding fresh cheeses (Standards INEN 1528 and 3067).   Keywords: Microbiological quality, Good Manufacturing Practices, fresh cheeses, legal regulations.  

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