Morphometric similarities and differences between tree genotype of Pramenka sheep from central Bosnia

Morphometric characterization of three strains: Dub, Privor and Kupres was done in order to obtain the genetic characterization of autochthonous sheep strains in Central Bosnia. Total of 205 ewes and rams was measured in order to determine similarities and differences between them. The eight, most important, morphometric trait were determined: wither height, rump height, body length, shoulder width, chest depth, hip width, chest perimeter and shin perimeter. Ewes of Dub Pramenka in relation to Privor and Kupres strains had pronounced morphometric measures, and established differences were statistically significant and highly significant. Statistically significant differences in all measures was observed between rams, expect for hip width. The obtained results show a significant difference in morphometric measures of three autochthonous Pramenka strains from Central Bosnia.

Download Full-text

Morphometric measurements as part of the genetic characterization of indigenous strain Kupreska Pramenka

Biotechnology in Animal Husbandry ◽

10.2298/bah1701055v ◽

2017 ◽

Vol 33 (1) ◽

pp. 55-64

Author(s):

Bozo Vazic ◽

Biljana Rogic ◽

Milanka Drinic ◽

Nebojsa Savic

Keyword(s):

Body Length ◽

Growth And Development ◽

Genetic Characterization ◽

The Body ◽

Average Height ◽

Morphometric Measurements ◽

Significant Difference ◽

Morphometric Characterization ◽

Correlative Relationship

For the purpose of genetic characterization of strains of sheep in Bosnia and Herzegovina, a morphometric characterization of Kupreska Pramenka has been performed. A total of 62 heads were measured, 56 ewes and 6 rams. The average height of the withers of ewes was 69.71 cm, the height of the hook was 70.57 cm, the body length was 72.57 cm, the chest width behind the shoulders was 21.12 cm, chest depth 31.98 cm, width of the hips was 20.28 cm, the chest volume was 90.95 cm and the circumference volume was 7.91 cm. The rams had an average height of 75.33 cm at the withers, the hook height of 76.33 cm, the body length 78.83 cm, the chest width behind the shoulders was 24.33 cm, chest depth 34.50 cm, width of the hips was 22.00 cm, chest volume was 98.50 cm and the circumference volume was 9.33 cm. In sheep and rams measures that have been shown the highest correlation and statistically highly significant difference were those related to the process of growth and development of the animal, and these are: the height of the withers and hook (r = 0.841 for ewes and r = 1.00 for rams), while the other hand, there are measures that do not show correlative relationship as hook height and chest volume (r = 0.155 for ewes and r = 0.533 for rams).

Download Full-text

Genetic characterization of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in Guangdong, China

Parasites & Vectors ◽

10.1186/s13071-019-3822-z ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 3

Author(s):

Jiayu Li ◽

Xiaoyu Dan ◽

Kexin Zhu ◽

Na Li ◽

Yaqiong Guo ◽

...

Keyword(s):

Risk Factors ◽

Genetic Characterization ◽

Giardia Duodenalis ◽

Ratio Analysis ◽

Infection Rates ◽

Sequence Analyses ◽

Chi Square ◽

Significant Difference ◽

Chi Square Test

Abstract Background There are only limited number of reports on molecular epidemiology of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in China. This study was conducted to assess the infection rates, genetic identity, and public health potential of these parasites in dogs and cats in Guangdong, China. Methods PCR and sequence analyses were used to identify and genotype Cryptosporidium spp. and G. duodenalis in fecal samples from 641 dogs and 418 cats in Guangdong. Chi-square test and odds ratio analysis were used to compare the occurrence rates of these pathogens and identify risk factors for infection. Results The overall infection rates of Cryptosporidium spp. and G. duodenalis were 6.9% (44/641) and 9.4% (60/641) in dogs, and 6.2% (26/418) and 3.6% (15/418) in cats. Purebred cats (12.4%; χ2 = 5.110, OR = 2.8, P = 0.024) and dogs (10.8%; χ2 = 5.597, OR = 4.8, P = 0.018) were more likely to be infected by Cryptosporidium spp. and G. duodenalis, respectively. Dogs (12.0%; χ2 = 7.589, OR = 2.6, P = 0.006) and cats (13.6%; χ2 = 8.235, OR = 3.5, P = 0.004) under 6 months had significantly higher infection rates of Cryptosporidium spp. than older animals. Household (13.9%; χ2 = 10.279, OR = 2.6, P = 0.008) and pet shop dogs (11.0%; χ2 = 7.182, OR = 2.0, P = 0.048) had higher occurrence of Cryptosporidium spp., as was the case for G. duodenalis occurrence in experimental dogs (13.4%; χ2 = 9.223, OR = 1.9, P = 0.017). Cryptosporidium canis (n = 42), C. muris (n = 1) and Cryptosporidium rat genotype IV (n = 1) were identified in dogs, while C. felis (n = 21), C. parvum (n = 3), C. muris (n = 1) and Cryptosporidium rat genotype IV (n = 1) were identified in cats. In contrast, the canine-specific assemblages C (n = 27) and D (n = 26) and the feline-specific assemblage F (n = 14) were almost exclusively the only genotypes of G. duodenalis in dogs and cats, respectively. There was no significant difference in infection rates of Cryptosporidium spp. and G. duodenalis between diarrheal and non-diarrheal pets. Conclusions While domestic pets in Guangdong are infected with zoonotic Cryptosporidium species, they are mainly infected with host-specific G. duodenalis genotypes. Risk factors for infections differ between Cryptosporidium spp. and G. duodenalis and between dogs and cats.

Download Full-text

Genetic diversity of Blastocystis in kindergarten children in southern Xinjiang, China

Parasites & Vectors ◽

10.1186/s13071-020-3890-0 ◽

2020 ◽

Vol 13 (1) ◽

Cited By ~ 5

Author(s):

Meng Qi ◽

Zilin Wei ◽

Ying Zhang ◽

Qiyuan Zhang ◽

Juanfeng Li ◽

...

Keyword(s):

Ribosomal Rna ◽

Genetic Characterization ◽

Intestinal Parasites ◽

Small Subunit ◽

Kindergarten Children ◽

Chain Reaction ◽

Significant Difference ◽

Polymerase Chain ◽

Southern Xinjiang

Abstract Background Blastocystis is one of the most common intestinal parasites in humans and various animals worldwide. Few studies are available regarding the genetic characterization of Blastocystis infections in humans in China. Methods In the present study, 609 fecal samples were collected from two- to six-year-old kindergarten children in southern Xinjiang and were examined by polymerase chain reaction (PCR). Results The infection rate of Blastocystis was 14.3% (87/609); no significant difference was observed among counties and between sexes. Blastocystis subtypes ST1 (n = 38), ST2 (n = 8), and ST3 (n = 41) were identified by sequence analysis of the small subunit ribosomal RNA gene. Genetic polymorphisms were observed at the intra-subtype level, including seven variations for ST1 (ST1A to ST1G), four for ST2 (ST2A to ST2D), and two for ST3 (ST3A and ST3B); with ST1F and ST2B being new variations. Conclusions ST1 and ST3 are the two common Blastocystis subtypes in the study area. More extensive studies in both humans and animals in different regions are needed to better characterize the transmission of Blastocystis.

Download Full-text

Morphometric characterization of Pirot pramenka

Biotechnology in Animal Husbandry ◽

10.2298/bah2102075r ◽

2021 ◽

Vol 37 (2) ◽

pp. 75-85

Author(s):

Dragana Ruzic-Muslic ◽

Milan Petrovic ◽

Bogdan Cekic ◽

Ivan Cosic ◽

Ivan Pavlovic ◽

...

Keyword(s):

Body Length ◽

The Body ◽

Average Height ◽

Body Frame ◽

Morphometric Traits ◽

Starting Point ◽

The Status ◽

Endangered Population ◽

Morphometric Characterization

The strategy of conservation of endangered sheep populations implies morphological and genetic characterization, as basic preconditions for their conservation. The aim of this study was to determine the morphometric characteristics, their correlations and the index of physical development of Pirot pramenka, which has the status of the most endangered population in Serbia. The measuring was performed on 30 sheep, aged 3 years, reared in the area of Stara Planina. The descriptive statistical procedure was performed using the statistical package Statistica (version 8). The average height at the withers was 56.31 cm, body length 62.93 cm, chest width 18.37 cm, chest depth 25.96 cm, chest circumference 77.59 cm, shin circumference 6.70 cm. The strongest and significantly positive correlation (P <0.05) was found between chest depth and height at withers (0.65), body length (0.58) and body weight (0.56). Pirotska pramenka has slightly higher indices of format, chest and massiveness, in relation to breeds Vitoroga Zuja and Travnik Pramenka. These morphometric traits suggest that, compared to previous research, the body frame of Pirot pramenka sheep did not change significantly, which means that there were no crosses with other breeds due to geographical isolation and enthusiasm of breeders to preserve the indigenous Pirot pramenka which was the starting point for following authentic brands: Pirot lamb, cheese and carpet. Hence the biological and moral imperative: to preserve this highly endangered population.

Download Full-text

Morphometric characterization of Katjang goat of Malaysia

Bangladesh Journal of Animal Science ◽

10.3329/bjas.v45i3.31035 ◽

2017 ◽

Vol 45 (3) ◽

pp. 17-24

Author(s):

MAMY Khandoker ◽

M Syafiee ◽

MSR Rahman

Keyword(s):

Body Weight ◽

Body Length ◽

Tail Length ◽

Head Length ◽

Head Width ◽

Strong Positive Correlation ◽

Leg Length ◽

Scrotal Circumference ◽

Morphometric Characterization

Morphometric characterization of Katjang goat in Malaysia is important for conservation and for further genetic improvement. With this idea in view the present study was carried out to characterize the morphometry and to estimate the relationship among the important morphometric traits of indigenous Katjang goat. The study was conducted at the Faculty of Sustainable Agriculture (FSA), surrounding area of FSA Sandakan, Sabah and Batu Pahat, Johor in Malaysia. Characteristics of body weight (BW), body length (BL), heart girth (HG), wither height (WH), hip height (HH), fore leg length (FLL), hind leg length (HLL), head length (HeL), head width (HW), ear length(EL), ear breadth (EB), tail length (TL), scrotal length (SL) and scrotal circumference (SC) were gathered. In a single parameter as body weight of Katjang bucks at below 1 year, 1-2 year and > 2 years ages were recorded to be 7.41 ± 0.27, 14.20±0.20 and25.83 ± 0.99 kg, respectively. On the hand, body weight of Katjang does at 1-2 year and > 2 years ages were observed to be 12.41 ± 0.41 and 23.65±0.87 kg, respectively. In both sexes, head length, head width, tail length, ear length, ear breath, body weight, body length, hearth girth, wither height, leg length, hip height increases with the increment of the age of goat. On the other hand, all the parameters contemplated found comparatively higher in bucks than that of does. Strong positive correlation was observed between scrotal length and scrotal circumference in Katjang bucks.Bang. J. Anim. Sci. 2016. 45 (3): 17-24

Download Full-text

The morphology and genetic characterization of Iheringascaris goai n. sp. (Nematoda: Raphidascarididae) from the intestine of the silver whiting and spotted catfish off the central west coast of India

Journal of Helminthology ◽

10.1017/s0022149x11000472 ◽

2011 ◽

Vol 86 (3) ◽

pp. 353-362 ◽

Cited By ~ 3

Author(s):

A. Malhotra ◽

N. Jaiswal ◽

A.K. Malakar ◽

M.S. Verma ◽

H.R. Singh ◽

...

Keyword(s):

New Species ◽

Body Length ◽

West Coast ◽

Closely Related Species ◽

West Coast Of India ◽

Significant Difference ◽

Fish Hosts ◽

Anal Papillae ◽

A New Species

AbstractIn this study a new species of nematode, Iheringascaris goai n. sp., is reported from two fish hosts, including silver whiting, Sillago sihama, and spotted catfish, Arius maculatus, caught off the Central West Coast of India at Goa. The new species can be differentiated morphologically from I. inquies, the most closely related species collected from cohabiting marine fish. The distinguishing characteristics are distinct cuticular striations, a unilateral excretory system, the presence of dentigerous ridges on the inner margin of the lips and the ratio of oesophagus to body length. In males, the ratio of spicules to body length is higher and the number of pre-anal papillae is less in comparison to those in I. inquies. In addition, the tail curves ventrad in males, while in females, the vulva is post-equatorial. The sequence alignment of 18S rDNA and cytochrome c oxidase subunit I with sequences of known species selected from the same superfamily shows a significant difference. The morphological and molecular differences reported here can, therefore, be used to assign the specimen to a new species.

Download Full-text

Study the Distribution of Rotavirus Genotypes in Vaccinated and non Vaccinated Children in Babylon Province

Iraqi Journal of Pharmaceutical Sciences ( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512) ◽

10.31351/vol30iss2pp167-176 ◽

2021 ◽

Vol 30 (2) ◽

pp. 167-176

Author(s):

Qassim Mahdi Mutlak ◽

Manal Khalid Abdulridha ◽

Laith M Abbas Al-Huseini

Keyword(s):

Genetic Characterization ◽

Rotavirus Vaccine ◽

Rt Pcr ◽

Rotavirus Gastroenteritis ◽

Rotavirus Vaccines ◽

National Immunization ◽

Significant Difference ◽

Polymerase Chain ◽

Age Range

Rotaviruses, a major cause of gastroenteritis in children worldwide accounts for around half a million deaths annually. Vaccine against the virus has been recommended by the WHO to be involved in the national immunization program.To evaluate the genetic characterization of rotavirus among children with acute gastroenteritis in Babylon province is warranted. Children complained of diarrhea with rotavirus infection detected in their stool were involved in the study. The age range was 10 months to 60 months and with retrogradechecking of rotavirus vaccine history. Rotavirus genotypes were detected by reverse transcription-polymerase chain reaction (RT-PCR).A total number of 40 children with rotavirus gastroenteritis were used to examine the rotavirus genotyping distribution. The prevalent genotypes were found to be G1, G9, and G2. No significant difference in the distribution of the length of diarrhea according to genotype. Vaccinated children exhibited significantly higher percentage of G2 while G1 and G9 were presented more considerably in not vaccinated children.We detected a variety of rotavirus strains includingthe major global genotypes.Obviously, rotavirus strains are continuing to vary in Iraq. The vaccination changes the genotype prevalence and this situation may create challenges to the effectiveness of rotavirus vaccines and planning for future polices. Keywords: Rotavirus, Vaccine, Genotype.

Download Full-text

Genetic Characterization of Endometriosis Patients: Review of the Literature and a Prospective Cohort Study on a Mediterranean Population

International Journal of Molecular Sciences ◽

10.3390/ijms21051765 ◽

2020 ◽

Vol 21 (5) ◽

pp. 1765 ◽

Cited By ~ 2

Author(s):

Stefano Angioni ◽

Maurizio Nicola D’Alterio ◽

Alessandra Coiana ◽

Franco Anni ◽

Stefano Gessa ◽

...

Keyword(s):

Genetic Characterization ◽

Allelic Frequency ◽

Minor Allele ◽

Control Group ◽

Mediterranean Population ◽

Heterozygous Genotype ◽

Significant Difference ◽

Sardinian Population ◽

Different Populations

The pathogenesis of endometriosis is unknown, but some evidence supports a genetic predisposition. The purpose of this study was to evaluate the recent literature on the genetic characterization of women affected by endometriosis and to evaluate the influence of polymorphisms of the wingless-type mammalian mouse tumour virus integration site family member 4 (WNT4), vezatin (VEZT), and follicle stimulating hormone beta polypeptide (FSHB) genes, already known to be involved in molecular mechanisms associated with the proliferation and development of endometriotic lesions in the Sardinian population. Materials and Methods: In order to provide a comprehensive and systematic tool for those approaching the genetics of endometriosis, the most cited review, observational, cohort and case-control studies that have evaluated the genetics of endometriosis in the last 20 years were collected. Moreover, 72 women were recruited for a molecular biology analysis of whole-blood samples—41 patients affected by symptomatic endometriosis and 31 controls. The molecular typing of three single nucleotide polymorphisms (SNPs) was evaluated in patients and controls: rs7521902, rs10859871 and rs11031006, mapped respectively in the WNT4, VEZT and FSHB genes. In this work, the frequency of alleles, genotypes and haplotypes of these SNPs in Sardinian women is described. Results: From the initial search, a total of 73 articles were chosen. An analysis of the literature showed that in endometriosis pathogenesis, the contribution of genetics has been well supported by many studies. The frequency of genotypes observed in the groups of the study population of 72 women was globally coherent with the law of the Hardy–Weinberg equilibrium. For the SNP rs11031006 (FSHB), the endometriosis group did not show an increase in genotypic or allelic frequency due to this polymorphism compared to the control group (p = 0.9999, odds ratio (OR) = 0.000, 95% confidence interval (CI), 0.000–15.000 and p = 0.731, OR = 1639, 95% CI, 0.39–683, respectively, for the heterozygous genotype and the polymorphic minor allele). For the SNP rs10859871 (VEZT), we found a significant difference in the frequency of the homozygous genotype in the control group compared to the affected women (p = 0.0111, OR = 0.0602, 95% CI, 0.005–0.501). For the SNP rs7521902 (WNT4), no increase in genotypic or allelic frequency between the two groups was shown (p = 0.3088, OR = 0.4133, 95% CI, 0.10–1.8 and p = 0.3297, OR = 2257, 95% CI, 0.55–914, respectively, for the heterozygous genotype and the polymorphic minor allele). Conclusion: An analysis of recent publications on the genetics of endometriosis showed a discrepancy in the results obtained in different populations. In the Sardinian population, the results obtained do not show a significant association between the investigated variants of the genes and a greater risk of developing endometriosis, although several other studies in the literature have shown the opposite. Anyway, the data underline the importance of evaluating genetic variants in different populations. In fact, in different ethnic groups, it is possible that specific risk alleles could act differently in the pathogenesis of the disease.

Download Full-text