scholarly journals Performance of GC-MS analysis for differentiation of various types of flour by creating dendrogram of liposoluble extract

2012 ◽  
Vol 18 (4-1) ◽  
pp. 555-561 ◽  
Author(s):  
Djura Vujic ◽  
Marijana Acanski ◽  
Marija Bodroza-Solarov ◽  
Nikola Hristov ◽  
Milica Krunic
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Cluster Analysis ◽  
Gas Chromatography ◽  
Methyl Oleate ◽  
Methyl Palmitate ◽  
Methyl Esters ◽  
Fatty Acids Composition ◽  
Different Types ◽  
Small Grains

Gas chromatography with mass spectrometry was used for performing a qualitative analysis of liposoluble flour extract made of different types of small grains (bread wheat, barley and triticale). The results show that the dominant methyl-esters of fatty acids composition is same for all samples, but the percentage of their representation in various types of small grains is different. Transesterification reagent was TMSH (Trimethylsulfonium hydroxide, 0.2M in methanol). With transesterification reaction fatty acids from acilglycerol esterifyed to methyl-esters. In all extract analyzed methyl linoleate predominated, followed by methyl palmitate, methyl oleate and methyl stearate in decreasing order. The following tests cluster analysis was used for the comparison of the liposoluble flour extract different types of small grains.

scholarly journals Qualitative analysis of hexane flour extract of spelt

2013 ◽  
pp. 125-130
Author(s):  
Djura Vujic ◽  
Djordje Psodorov ◽  
Marijana Acanski ◽  
Marija Bodroza-Solarov ◽  
Jovana Brkljaca
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Cluster Analysis ◽  
Gas Chromatography ◽  
Qualitative Analysis ◽  
Methyl Palmitate ◽  
Methyl Esters ◽  
Predominant Component ◽  
Three Samples ◽  
Different Types

Gas chromatography with mass spectrometry (GC-MS) was used for performing a qualitative analysis of the hexane flour extract of three samples of spelt. All the three samples were first treated with hexane and the obtained extracts were used for the analysis of the fatty acid lipid components. The transesterification reaction was performed using TMSH (trimethylsulfonium hydroxide, 0.2M in methanol), and the fatty acids were esterified from acylglycerol to methyl-esters. In all analyzed extracts, the predominant component was methyl linoleate, followed by methyl oleate and methyl palmitate. The subsequent tests, performed by cluster analysis, were used to compare the hexane flour extracts of different types of spelt.

Direct ultrasound-assisted methylation of fatty acids in serum for free fatty acid determinations

2010 ◽  
Vol 88 (9) ◽  
pp. 898-905 ◽  
Author(s):  
Liyan Liu ◽  
Ying Li ◽  
Rennan Feng ◽  
Changhao Sun
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Ultrasonic Waves ◽  
Acid Methyl ◽  
Ultrasound Assisted

A method for simultaneous determination of 16 free fatty acids (FFAs) in serum is described. The method involves conversion of FFAs to fatty acid methyl esters (FAMEs) using the heat of ultrasonic waves followed by gas chromatography and mass spectrometry (GC–MS) analysis. Optimum levels of the variables affecting the yield of FAMEs were investigated. The results indicate that the optimal levels are 55 °C, 60 W, 10% H2SO4/CH3OH, and 50 min. Recoveries ranged from 85.32% to 112.11%, with a detection limit ranging from 0.03 to 0.08 μg mL–1. The linearity, using the linear correlation coefficient, was higher than 0.9914.

Evaluation of volatile compounds in different types of ghee using direct injection with gas chromatography-mass spectrometry

2002 ◽  
Vol 69 (1) ◽  
pp. 163-171 ◽  
Author(s):  
UDAY R. WADODKAR ◽  
JAGJIT S. PUNJRATH ◽  
AMRISH C. SHAH
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Volatile Compounds ◽  
Direct Injection ◽  
Gas Chromatography Mass Spectrometry ◽  
Local Market ◽  
Butter Oil ◽  
Chromatography Mass Spectrometry ◽  
Different Types

Desi ghee (DG) was prepared from fermented cream followed by heat clarification (desi method) in the laboratory and butter oil (BO) was prepared from fresh butter by melting and centrifugation. Fresh samples of three brands of industrial ghee (IG-1, IG-2, IG-3) were collected from the local market. Volatile compounds of desi and industrial ghee and butter oil were isolated and concentrated using direct injection and cryofocussing techniques; separation and identification was by Gas Chromatography Mass Spectrometry (GCMS). A maximum of 36 compounds were detected in desi ghee whereas compounds detected in three industrial ghee samples varied from 22–29. The lowest number of compounds (16) was detected in butter oil. Of the identified compounds, maltol, 5-hydroxymethyl furfuraldehyde, dihydrodihydroxypyranone, 1,3-butanediol and 1-octanol were identified only in desi ghee volatiles. The concentration of acetic acid was found to be remarkably higher in desi ghee volatiles than in industrial ghee. Also the levels of identified fatty acids, methyl ketones, aldehydes, lactones and alcohols were high in desi ghee volatiles compared with industrial ghee and butter oil. In total, 62 compounds were detected, which included 6 aldehydes, 12 ketones, 8 each of fatty acids, alcohols and lactones, 4 each of esters and hydrocarbons or other compounds, and 12 compounds remained unidentified.

scholarly journals Homogenity of oil and sugar components of flour amaranth investigated by GC-MS

2015 ◽  
Vol 21 (1-1) ◽  
pp. 71-76
Author(s):  
Djordje Psodorov ◽  
Marijana Acanski ◽  
Djura Vujic ◽  
Jovana Brkljaca ◽  
Dragan Psodorov
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Methyl Esters ◽  
Soluble Sugars ◽  
Soluble Carbohydrates ◽  
Three Samples ◽  
Hexane Extracts ◽  
Ethanol Extracts ◽  
Ethanol Ethanol

Gas chromatography with mass spectrometry (GC-MS) was used for performing a qualitative analysis of liposoluble and hydrosoluble flour extracts of three genotypes of Amaranthus sp. All three samples were first defatted with hexane. Hexane extracts were used for the analysis of fatty acids of lipid components. TMSH (Trimethylsulfonium hydroxide, 0.2M in methanol) was used as the transesterification reagent. With transesterification reaction, fatty acids were esterified from acilglycerol to methyl-esters. Defatted flour samples were dried in the air and then extracted with ethanol. Ethanol extracts were used for the analysis of soluble carbohydrates. TMSI (trimethylsilylimidazole) was used as a reagent for the derivatization of carbohydrates into trimethylsilylethers. The results show that the dominant methyl-esters of fatty acids are very similar in all the three samples. Such a similarity was not detected in the analysis of soluble sugars. The following test cluster analysis was used for the comparison of liposoluble and hydrosoluble flour extracts of three genotypes of Amaranthus sp.

scholarly journals Composition Characterization of Fatty Acid Zinc Salts by Chromatographic and NMR Spectroscopic Analyses on Their Fatty Acid Methyl Esters

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Kwang Seo Park ◽  
Yun Ju Kim ◽  
Eun Kyung Choe
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
Acid Methyl ◽  
Zinc Salts

To implement EU REACH- (Registration, Evaluation, and Authorization of Chemicals-) like chemical legislations in various countries of which the purpose is human and environment safety, the first step is substance identification followed by the hazard and risk assessments. Although both structural and composition identifications are required, the latter can more importantly result in the essential data to fill out the required substance information such as purity and concentrations of constituents, as well as impurities. With fatty acid zinc salts (FAZSs) as an exemplary industrial chemical of which chromatographic and nuclear magnetic resonance (NMR) analyses were impossible due to their insolubility in water and any organic solvents, the composition characterization was tried by preparing their fatty acid methyl esters (FAMEs) using the conc. HCl/methanol/toluene method. This acid-catalyzed methyl esterification was optimized with zinc stearate as a surrogate substance. Gas chromatography-mass spectrometry (GC-MS) and NMR analyses on methyl-esterified products revealed that the optimum conditions were at 90°C for 10 min or 45°C for 30 min with two equivalent HCl as well as at 45°C for 10 min with five equivalent HCl. Almost all zinc stearates were converted into the corresponding fatty acids with 97–99% conversion rates. Free fatty acids (FFAs) were detected in extracted ion chromatograms of pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) in the methyl-esterified products with incomplete conversions of 73∼79%. The optimized conc. HCl/methanol/toluene method of direct one-step reaction from FAZSs was compared with the two-step NaOH saponification/BF3-methanol method after acidic hydrolysis of FAZSs. The mechanism of fatty acid zinc salts into free fatty acids and fatty acid methyl esters was suggested with the evidence of the formation of Zn(OH)2.

scholarly journals Evaluation of Non-polar Composition in Plumbago Zeylanica Leaves by Gas Chromatography and Mass Spectrometry

Folia Medica ◽  
2020 ◽  
Vol 62 (2) ◽  
pp. 308-313
Author(s):  
Jayesh Dhalani ◽  
Gaurang Dubal ◽  
Chirag Rathod ◽  
Pankaj Nariya
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Chemical Compounds ◽  
Mass Spectrometry Analysis ◽  
Plumbago Zeylanica ◽  
Acid Methyl

Background:Plumbago zeylanica plant belongs to Plumbaginaceae. The plant is reported for many pharmacological activities. Aim: The objective of the study was to identify fatty acids and non-polar chemical compounds in Plumbago zeylanica leaves. Materials and methods: Petroleum ether extract was prepared using soxhlet apparatus. Saponifiable and unsaponifiable matter was separated with saponification process. To identify fatty acids in saponifiable matter further esterification was performed. Gas chromatography and mass spectrometry analysis was performed of both saponifiable and unsaponifiable fractions. All the fatty acid methyl esters and non-polar chemical compounds were identified using NIST library data. Results: A total of 14 compounds were identified with comparison of NIST data. From that, 8 fatty acid methyl esters and 6 non-polar chemical compounds were identified. Here we have analyzed fatty acids and non-polar chemical compounds by the same GC-MS method. Conclusions: The present analysis showed that Plumbago Zeylanica leaves contain 8 fatty acids and 6 non-polar chemical compounds. Principal determination of the research was development of efficient method to identify non-polar compound from plant by single injection using chromatographic technique.

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