scholarly journals Transformation, evaluation of GTGene and multivariate genetic analysis for morpho-physiological and yield attributing traits in Zea mays

Genetika ◽  
2016 ◽  
Vol 48 (1) ◽  
pp. 423-433 ◽  
Author(s):  
Khadija Aaliya ◽  
Zahida Qamar ◽  
Nasir Ahmad ◽  
Qurban Ali ◽  
Farooq Munim ◽  
...  
Keyword(s):  
Grain Yield ◽  
Abiotic Factors ◽  
Glyphosate Resistance ◽  
Field Trials ◽  
Yield Potential ◽  
Chlorophyll Contents ◽  
Synthetic Varieties ◽  
Green Fodder ◽  
Transgenic Lines ◽  
Fodder Yield

Maize is the 3rd major crop grown all over the world that fulfills the needs of millions of people. Various biotic and abiotic factors caused reduction in grain yield of maize, among them weeds show most adverse effects. Objective of this study was to develop glyphosate resistance maize for developing maize hybrids and synthetic varieties with high grain and fodder yield potential. Glyphosate is a broad spectrum herbicide and resistance of crop against the herbicide, allows post emergence application, which otherwise is impossible. Higher GTG crude protein (479.94?g/g) was recorded for CIL 194-975 plant. The plant CIL 194-1786 showed higher expression level of GTG protein, indicated that transformation efficiency was better for line CIL-194 than CIL-123. GTGene stably integrated to the genome of the maize lines CIL-194 and CIL-123, these transformed lines showed more ability to cope with herbicides as compared with non-transformed lines in greenhouse and field trials. The transgenic lines were sown in field and data was recorded for various morpho-physiological, grain, fodder yield and quality traits. Data was statistically analyzed to check significance of results at <0.05% probability level. It was concluded from heritability, genetic advance, stepwise multiple linear regression, principle component and factor analysis that the selection on the basis of grain yield, green fodder yield, stem diameter, cob weight, stomata conductance, chlorophyll contents, photosynthetic rate, cob length, grain protein, grain oil, embryo percentage and grain starch may be fruitful keeping in view as the major contributing traits to improve crop yield and production. It was suggested that the transgenic lines for glyphosate resistance may be used to develop transgenic hybrids and synthetic varieties with higher grain yield with high quality.

Study of green fodder yield potential of pearl millet genotypes under rainfed conditions of Gujarat, India

2016 ◽  
Vol 36 (2) ◽  
Author(s):  
K. K. Dhedhi ◽  
V. V. Ansodariya ◽  
N. N. Chaudhari ◽  
J. S. Sorathiya
Keyword(s):  
Grain Yield ◽  
Pearl Millet ◽  
Plant Population ◽  
Yield Potential ◽  
Research Station ◽  
Rainfed Conditions ◽  
Days To Maturity ◽  
Green Fodder ◽  
Fodder Yield ◽  
Grassland Research

Twenty three fodder pearl millet genotypes including two checks <italic>viz</italic>., DFMH 30 and PAC 981 were evaluated at Pearl millet Research Station, Junagadh Agricultural University (JAU), Jamnagar and Grassland Research Station, JAU, Dhari during <italic>kharif</italic> 2014. Significant differences were observed among the genotypes for days to 50 % flowering, days to maturity, plant height, dry fodder yield and green fodder yield while differences for plant population and grain yield were non significant. The locations differences were found significant for all the characters studied. The genotypes x locations differences were observed non significant for all the traits except plant population and grain yield. Days to 50 % flowering varied from 52 days (DFMH 30) to 77 days (IP 15564). Days to maturity ranged between 75 days (IP 2761) to 96 days (IP 14542). IP 22269 (342 cm) was observed the tallest genotype, while, check DFMH 30 (252 cm) was manifested the shortest genotype. Dry fodder yield ranged from 96 q/ha (IP 5153, IP 2761) to 208 q/ha (ICMV 05222). The genotype ICMV 05222 (390 q/ha) produced the highest green fodder yield among all the genotypes followed by IP 6202 (348 q/ha) and IP 15564 (341 q/ha). Hence, these three genotypes <italic>viz</italic>., ICMV 05222, IP 6202 and IP 15564 may be considered for general cultivation under rainfed conditions of Gujarat.

Evaluation of Different Varieties of Pearl Millet for Green Fodder Yield Potential

2002 ◽  
Vol 1 (4) ◽  
pp. 326-327 ◽  
Author(s):  
Muhammad Naeem ◽  
Muhammad Shahid Muni . ◽  
Ahmad Hasan Khan . ◽  
Sultan Salahuddin .
Keyword(s):  
Pearl Millet ◽  
Yield Potential ◽  
Green Fodder ◽  
Fodder Yield

scholarly journals Assessment of Maize Genotypes for Forage Yield and Quality

2018 ◽  
Vol 6 (1) ◽  
pp. 09-14
Author(s):  
Abdul Basit ◽  
Abdul Khaliq ◽  
Asim Pervez ◽  
Sikandar Hayat ◽  
Muhammad Saleem Akhtar
Keyword(s):  
Nutritional Quality ◽  
Block Design ◽  
Weight Ratio ◽  
Leaf Length ◽  
Forage Yield ◽  
Yield Potential ◽  
Crude Fibre ◽  
Maize Genotypes ◽  
Green Fodder ◽  
Fodder Yield

The present study was targeted to evaluate eight maize advance lines in comparison to one local check for green fodder yield potential and nutritional quality. The experiment was conducted at Fodder Research Institute, Sargodha during August 2017. The experiment was sown in a randomized complete block design with three replications having a plot size of 2.4 m x 6 m. The crop was harvested at 50% flowering stage and data regarding plant height, Number of leaves per plant, leaf length, leaf width, leaf area, leaves weight per plant, stem weight per plant, leaves to stem weight ratio, green fodder yield, dry matter percentage, crude protein and crude fibre were recorded. The results showed that the performance of the advance lines; No.1501 and MS.2010 was best among all the studied genotypes in terms of fodder yield respectively. Both genotypes exhibited a good nutritional quality.

scholarly journals Combining Protein Content and Grain Yield by Genetic Dissection in Bread Wheat under Low-Input Management

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1058
Author(s):  
Junjie Ma ◽  
Yonggui Xiao ◽  
Lingling Hou ◽  
Yong He
Keyword(s):  
Grain Yield ◽  
Protein Content ◽  
Bread Wheat ◽  
Growing Season ◽  
Triticum Aestivum L ◽  
Field Trials ◽  
Yield Potential ◽  
Wheat Varieties ◽  
Low Input ◽  
Conventional Management

The simultaneous improvement of protein content (PC) and grain yield (GY) in bread wheat (Triticum aestivum L.) under low-input management enables the development of resource-use efficient varieties that combine high grain yield potential with desirable end-use quality. However, the complex mechanisms of genotype, management, and growing season, and the negative correlation between PC and GY complicate the simultaneous improvement of PC and GY under low-input management. To identify favorable genotypes for PC and GY under low-input management, this study used 209 wheat varieties, including strong gluten, medium-strong gluten, medium gluten, weak gluten, winter, semi-winter, weak-spring, and spring types, which has been promoted from the 1980s to the 2010s. Allelic genotyping, performed using kompetitive allele-specific polymerase chain reaction (KASP) technology, found 69 types of GY-PC allelic combinations in the tested materials. Field trials were conducted with two growing season treatments (2018–2019 and 2019–2020) and two management treatments (conventional management and low-input management). Multi-environment analysis of variance showed that genotype, management, and growing season had extremely substantial effects on wheat GY and PC, respectively, and the interaction of management × growing season also had extremely significant effects on wheat GY. According to the three-sigma rule of the normal distribution, the GY of wheat varieties Liangxing 66 and Xinmai 18 were stable among the top 15.87% of all tested materials with high GY, and their PC reached mean levels under low-input management, but also stably expressed high GY and high PC under conventional management, which represents a great development potential. These varieties can be used as cultivars of interest for breeding because TaSus1-7A, TaSus1-7B, TaGW2-6A, and TaGW2-6B, which are related to GY, and Glu-B3, which is related to PC, carry favorable alleles, among which Hap-1/2, the allele of TaSus1-7A, and Glu-B3b/d/g/i, the allele of Glu-B3, can be stably expressed. Our results may be used to facilitate the development of high-yielding and high-quality wheat varieties under low-input management, which is critical for sustainable food and nutrition security.

scholarly journals Leaf Clipping and Population Density Effects on Green Fodder and Grain Yield for Hybrid Maize (Zea mays) in Bangladesh

2019 ◽  
Vol 7 (2) ◽  
pp. 55-62
Author(s):  
Golam M. Rokon ◽  
Md.Mahmudul H. Khan ◽  
Md.Maniruzzaman Bahadur ◽  
Maruf H. Shanto ◽  
Md.Khairul Alam ◽  
...  
Keyword(s):  
Population Density ◽  
Grain Yield ◽  
Harvest Index ◽  
Smallholder Farmers ◽  
Block Design ◽  
Research Field ◽  
Yield Attributes ◽  
Leaf Clipping ◽  
Green Fodder ◽  
Fodder Yield

Smallholder farmers having fragmented lands need fodder and grains simultaneously for earning food security for their families. A study was conducted in Crop Physiology and Ecology Research Field and Laboratory at Hajee Mohammad Danesh Science and Technology University, Bangladesh during the period of March to July-2013 to investigate the effect of leaf clipping and population density on fodder and grain yield in maize. Three population densities (D1= 75 cm × 25 cm, D2= 60 cm × 20 cm and D3= 50 cm × 20 cm) and three clipping treatments (C1 = no clipping, C2 = removal of all leaf blades below the lowermost cob and C3 = removal of all leaf blades above the uppermost cob) at the silking stage were included as experimental treatments. The experiment was laid out in a two factors Randomized Complete Block Design (RCBD) with three replications. Results revealed that D1 required the maximum days to attain most of the phenological stages of maize. Higher population density (D3) with C3 clipping treatment gave the highest plant height, whereas D1 with non-clipping treatment gave the lowest. Highest total dry matter (TDM) was found in D2 with C1 and the lowest was found in D1 with C1 treatment. The highest yield (8.88 t ha-1) and harvest index (36.2%) were found in D3 treatment whereas the lowest yield (5.92 t ha-1) in D1 population density but harvest index (32.6 %) was lowest in D2. The highest yield (8.33 t ha-1) and harvest index (35.5 %) were obtained from C1 treatment and the lowest yield (6.55 t ha-1) and harvest index (33.5 %) were obtained from C3 treatment. The highest fodder yield (3.33 t ha-1) was obtained from D3 treatment and the lowest (2.11 t ha-1) in D1 treatment. In C2 treatment, the highest amount of fodder (4.67 t ha-1) was obtained. The interaction between population density and leaf clipping treatment showed a significant variation among the yield and yield attributes in maize. It is indicated that D3 and C1 combination showed the best performance in respect of grain yield (9.67 t ha-1) and harvest index (38.3 %) of maize. But for both grain and fodder yield, D3 with C2 showed the best performance.

scholarly journals Evaluation of Glyphosate Resistance in Transgenic Lettuce

2000 ◽  
Vol 125 (6) ◽  
pp. 669-672 ◽  
Author(s):  
Russell T. Nagata ◽  
Joan A. Dusky ◽  
Robert J. Ferl ◽  
Antonio C. Torres ◽  
Daniel J. Cantliffe
Keyword(s):  
Dry Weight ◽  
Glyphosate Resistance ◽  
Field Trials ◽  
Growth And Yield ◽  
Transgenic Lines ◽  
Assay Methods ◽  
Independent Transformation ◽  
Identify Gene Expression ◽  
Leaf Disks ◽  
Herbicide Glyphosate

Six transgenic `South Bay' lettuce lines (Lactuca sativa L.) with elevated levels of 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) were evaluated for tolerance to the herbicide glyphosate. The six lines were selected from ≈150 independent transformation events using an Agrobacterium tumefaciens system. Three assay methods were used to identify gene expression with regard to glyphosate resistance. Leaf disks of the transgenic lines were cultured on media containing 0 to 1280 μm glyphosate. Leaf disks of the control had lower dry weight (DW) at 40 μm and greater glyphosate than all the transgenic lines. The transgenic lines continued to grow even at 1280 μm. Plants 21 days old were sprayed in the greenhouse with rates of glyphosate at 0 to 35.84 kg·ha-1. DW of all the lines were similar to the control, with a few exceptions, at glyphosate concentrations from 0 to 0.56 kg·ha-1. At 2.24 to 8.96 kg·ha-1 all of the transgenic lines had DW greater than the control, while at 17.92 and 35.84 kg·ha-1 only B-32, B-33, C-3, and C-14 had DW greater than the control. The resistant line from the greenhouse experiment, B-32, grew normally in field trials at the highest glyphosate rate, 17.92 kg·ha-1, while control plants died at 0.56 kg·ha-1 glyphosate. Lines A-11 and C-3 had lower DW than B-32 at 2.24 kg·ha-1 glyphosate and greater. While leaf disk assays can identify potential transformed lines expressing the EPSPS and glyphosate oxidase (GOX) gene, and greenhouse screening can evaluate seedling vigor after glyphosate application, field trials are necessary to evaluate plant growth and yield through the growing season. Chemical name used: N-(phosphono-methyl) glycine (glyphosate).

scholarly journals Effect of Last Cutting Dates on Seed Production of Multicut MB-87 – A Variety of Pearl Millet, Pennisetum glaucum (Bajra)

2018 ◽  
Vol 51 (2) ◽  
pp. 67-73
Author(s):  
A.H. Shah ◽  
H. Karar ◽  
M.S. Farooq ◽  
M.S.A. Bazmi ◽  
G. Nabi ◽  
...  
Keyword(s):  
Grain Yield ◽  
Pearl Millet ◽  
Seed Production ◽  
Pennisetum Glaucum ◽  
Farming System ◽  
Tropical Regions ◽  
Green Fodder ◽  
Fodder Yield ◽  
Food And Nutritional Security ◽  
Research Institute

Abstract Pearl millet (Pennisetum glaucum) is considered an important kharif crop, which is grown extensively in the arid and semi-arid tropical regions, where other crops, like sorghum and maize, fail to produce economic yields. Its grains are valued as human food, while its dry stover makes significant livestock ration in croplivestock farming system. Pearl millets (MB-87), a newly developed variety of Fodder Research Institute, Sargodha, Pakistan, is one of the best pearl millet variety, which gives more than one cutting and helps to cope with fodder lean period, as well as more income can be generated in addition to seed. So, overcome the scarcity period the study was conducted to check the effect of last cutting dates on seed production of multicut pearl millet MB-87 was conducted at Fodder Research Institute, Sargodha, Pakistan, during 2015 and 2016. Following parameters were taken in to consideration, i.e. plant height (cm), stem thickness (cm), number of leaves per tiller, number of tillers per plant, green fodder yield (t/ha), length of head (cm), 1000 grains weight (g) and grain yield (kg/ha). The results depicted that grain yield, i.e. 319.20, was maximum and head length was highest, i.e. 35.13 cm on 20th of August having last date of cut, whereas all others parameters were found to be nonsignificant. Multicut bajra gives three cutting of fodder, followed by seed, as compared with conventional bajra, which gives only one cutting or seed. Therefore, it is more economical for the growers of fodders to grow the pearl millets MB-87 as fodder, as well as for seed purposes. So, it is concluded that for gaining maximum grain yield of multicut pearl millet MB-87 the last cutting date should be 20th of August. In future, pearl millet is likely to play a larger role in providing food and nutritional security.

Yield improvement and associated characteristics of some Australian spring wheat cultivars introduced between 1860 and 1982

1989 ◽  
Vol 40 (3) ◽  
pp. 457 ◽  
Author(s):  
MW Perry ◽  
MF D'Antuono
Keyword(s):  
Grain Yield ◽  
Western Australia ◽  
Harvest Index ◽  
Triticum Aestivum L ◽  
Field Trials ◽  
Grain Weight ◽  
Yield Potential ◽  
Rate Of Increase ◽  
Australian Wheat ◽  
1000 Grain Weight

Twenty-eight Australian wheat (Triticum aestivum L. em. Thell.) cultivars representing a series from the 1860s to 1982, were grown in 20 field trials over four years in the wheatbelt of Western Australia. The cultivars included introductions and selections made before 1900, plus important cultivars bred or grown in Western Australia up to 1982. Five of the latter group were from crosses including semidwarf cultivars as parents. Grain yields were measured on all trials, and six trials were also sampled for biomass and yield components.Based on the regression of mean grain yield versus the number of years elapsed since 1884, yields have increased from 1022 kg ha-1 in 1884 to 1588 kg ha-1 in 1982. This represents a rate of increase of 5.8 kg ha-1 year-1 or 0.57% per year. Regression of cultivar yield on site mean yield gave values of b, the slope of the regression, from 0.66 to 1.24, and these were higher for modern than for old cultivars.In six trials sampled for yield components, above-ground biomass appeared to have increased slightly when comparing early selections and their derivatives with later cultivars, but over 80% of the overall increase in grain yield was due to increase in harvest index. Grains per car and grains m-2 were strongly and positively correlated with grain yield, but there were weak negative correlations between 1000-grain weight and yield, and between 1000 grain weight and years since 1884. Cultivars with a semi-dwarf background had equal biomass, but higher yield, harvest index, ear number m-2 and grains ear-2 than modern tall cultivars. The results show that genetic improvement has substantially increased yield potential in this environment and that this has been achieved through substantial increases in grain number m-2 associated with an improvement in harvest index.

scholarly journals Assessment of genetic diversity in germplasm of Guinea grass (Panicum maximum Jacq.)

2019 ◽  
Author(s):  
P. Ramakrishnan ◽  
C. Babu ◽  
K. Iyanar ◽  
N. Manivannan
Keyword(s):  
Genetic Diversity ◽  
Mean Value ◽  
Yield Potential ◽  
Breeding Programme ◽  
Panicum Maximum ◽  
Yield And Quality ◽  
Cluster Distance ◽  
Green Fodder ◽  
Fodder Yield ◽  
Guinea Grass

In the present study, sixty genotypes of Guinea grass were evaluated for assessing genetic diversity for ten different quantitative characters for exploitation in a breeding programme aimed at improving yield potential of Guinea grass by using Mahalanobis D2 statistics. The genotypes were grouped into ten clusters suggesting the presence of genetic diversity. The cluster I had maximum of 30 genotypes followed by II and III having 15 and 7 genotypes, respectively. These clusters having maximum number of genotypes, reflecting narrow genetic diversity. The inter cluster distances were greater than intra cluster distances, revealing that the selected genotypes were highly divergent. The maximum intra cluster distance was recorded for cluster III (5.63) while clusters IV, V, VI, VII and VIII and X (0.00) were solitary and showed no intra-cluster distance values. The genetically more divergent genotypes present in cluster III and IX as indicated by inter cluster distance value (21.63). Cluster VIII and Cluster V had least number of single genotype and emerged with contained highest cluster mean value for number of tillers/plant, number of leaves/plant, good leaf weight, leaf: stem ratio, green fodder yield/plant and crude protein content. Hence, GGLC 12 genotype in cluster VIII and GGLC 19 in cluster V can be successfully utilized in breeding programme for development of Guinea grass varieties with improved fodder yield and quality.

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