scholarly journals A molecular genetic approach to roebuck individual identification in the case of poaching in Serbia

2013 ◽  
Vol 67 (3-4) ◽  
pp. 279-287
Author(s):  
Vladimir Dimitrijevic ◽  
Ruzica Trailovic ◽  
Branko Petrujkic ◽  
Mila Savic ◽  
Predrag Simeunovic ◽  
...  
Keyword(s):  
Molecular Genetic ◽  
Geographic Origin ◽  
Capreolus Capreolus ◽  
Forensic Analysis ◽  
Individual Identification ◽  
Meat Sample ◽  
Hunting Season ◽  
Game Warden ◽  
First Case ◽  
Valid Evidence

Application of the molecular genetic methods in forensic cases dealing with wild animals has significantly increased recently. These techniques are practically used in order to help solving four key problems : determination of kind of the wild animal, geographic origin, kinship ties and individual identification. In this work the first case of introducing the examination of polimorphism of microsatelite genetic markers within forensic analysis in the cases of poaching in Serbia is presented. The objectives of this forensic analysis was to determine if the meat confiscated during house search of the suspect comes from roebuck origin (Capreolus capreolus), which remains had been found by a game warden in the field during closed season, where the suspect denied the offense, claiming that the meat comes from other roebuck that had been shot during the previous hunting season. DNK was isolated from the skin and fur samples taken from the roebuck corpse found in the woods, as well as from the frozen meat found in the suspect?s house. Both amplification and polimorphism examination of the eight microsatelite markers (ROE01, NVHRT21, NVHRT24, NVHRT48, NVHRT73, RT7 AND RT27) were carried out. In all the examined samples, the same pattern of variability of the tested microsatelites was determined, that is it was proved that DNK profiles of the samples taken from roebuck corpse were identical to DNK profile of the meat sample found in the suspect?s house. This result clearly indicates that all the examined biological samples originate from the same animal, and consequently represents forensically valid evidence in the case of roebuck poaching.

Bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus)

2014 ◽  
Vol 17 (2) ◽  
pp. 315-319 ◽  
Author(s):  
A. Bancerz-Kisiel ◽  
A. Szczerba-Turek ◽  
A. Platt-Samoraj ◽  
P. Socha ◽  
W. Szweda
Keyword(s):  
Cervus Elaphus ◽  
Red Deer ◽  
Roe Deer ◽  
Capreolus Capreolus ◽  
Hunting Season ◽  
Virulence Markers ◽  
Serotype O ◽  
Gene Coding ◽  
Environmental Reservoir ◽  
Enterocolitica Infection

AbstractFree-living animals are an important environmental reservoir of pathogens dangerous for other animal species and humans. One of those is Yersinia (Y.) enterocolitica, the causative agent of yersiniosis - foodborne, enzootic disease, significant for public health. The purpose of the study was to identify bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus) obtained during the 2010/2011 hunting season in north-eastern Poland. From among 48 rectal swabs obtained from 24 roe deer, two strains of Y. enterocolitica from one animal were isolated. Although both belonged to biotype 1A they were identified as different serotypes. The strain obtained from cold culture (PSB) belonged to serotype O:5, while the strain isolated from warm culture (ITC) was regarded as nonidentified (NI), what may suggest mixed infection in that animal. The presence of ystB gene, coding for YstB enterotoxin, directly related to Y. enterocolitica pathogenicity was detected in both strains using triplex PCR. The effect of the examination of 32 swabs obtained from 16 red deer was the isolation of two Y. enterocolitica strains from two different animals. Both belonged to biotype 1A with NI serotype, but were originated from different types of culture. They gave positive results in case of products of a size corresponding to the ystB gene. No amplicons corresponding to ail and ystA genes were found. Roe deer and red deer may carry and shed Y. enterocolitica, what seems to be important in aspect of an environmental reservoir of this pathogen. The Y. enterocolitica strains isolated from wild ruminants had the amplicons of the ystB gene, what suggest they can be potential source of Y. enterocolitica infection for humans

scholarly journals The first report of massive infestation with Lipoptena Cervi ( Diptera: Hippoboscidae ) in Roe Deer ( Capreolus Capreolus ) in Iasi county, N-E of Romania

2017 ◽  
Vol 69 (2) ◽  
pp. 293-298 ◽  
Author(s):  
M. Lazăr ◽  
O.C. Iacob ◽  
C. Solcan ◽  
S.A. Pașca ◽  
R. Lazăr ◽  
...  
Keyword(s):  
Roe Deer ◽  
Morphological Characteristics ◽  
Capreolus Capreolus ◽  
Alcoholic Solution ◽  
The Body ◽  
Body Parts ◽  
Hunting Season ◽  
Inflammatory Reactions ◽  
Trophy Hunting ◽  
The Face

ABSTRACT Investigations of four roe deer corpses were carried out from May until October 2014, in the Veterinary Forensic Laboratory and in the Parasitic Diseases Clinic, in the Iasi Faculty of Veterinary Medicine. The roe deer were harvested by shooting during the trophy hunting season. The clinical examination of the shot specimens revealed the presence of a highly consistent number of extremely mobile apterous insects, spread on the face, head, neck, lateral body parts, abdominal regions, inguinal, perianal and, finally, all over the body. The corpses presented weakening, anemia and cutaneous modification conditions. Several dozen insects were prelevated in a glass recipient and preserved in 70º alcoholic solution in order to identify the ectoparasite species. The morphological characteristics included insects in the Diptera order, Hippoboscidae family, Lipoptena cervi species. These are highly hematophagous insects that by severe weakening are affecting the game health and trophy quality. Histological investigations of the skin revealed some inflammatory reactions caused by ectoparasite Lipoptena cervi. Lipoptena cervi was identified for the first time in Iasi County, Romania.

Forensic Matter

2020 ◽  
pp. 81-120
Author(s):  
Ángeles Donoso Macaya
Keyword(s):  
Forensic Analysis ◽  
Abandoned Mine ◽  
Documentary Films ◽  
Forensic Evidence ◽  
First Case ◽  
The Media

The Lonquén case, as it is known, was the first case that definitively confirmed the denied existence of the detained-disappeared. Besides establishing that fifteen men who had been detained on October 7, 1973, had been thrown into the furnace of an abandoned mine, buried, and left there in clandestine cemeteries, the forensic analysis and the inspecting judge’s investigation also revealed that the depositions given by the policemen involved in the crime were completely false. Despite the prominence of the Lonquén case, little attention has been placed on the documentary photographs that served to secure and present the physical remains found as forensic evidence. I consider two sets of photographs taken by Vicaría de la Solidaridad photographers Helen Hughes and Luis Navarro at the abandoned mine during the first days of December 1978, immediately after the findings. My analysis centers on the photos that were disseminated publicly and also considers how the unpublished photos (the vast majority) were discussed in the media. The chapter ends with a brief consideration of No Olvidar (Not To Forget) (1982) by Ignacio Agüero, and La ciudad de los fotógrafos by Sebastián Moreno, two documentary films in which the traces of Lonquén continue emerging.

Individual identification of Amur leopards (Panthera pardus orientalis) using molecular-genetic methods and the population size estimation

2013 ◽  
Vol 40 (2) ◽  
pp. 124-129 ◽  
Author(s):  
V. V. Rozhnov ◽  
P. A. Sorokin ◽  
V. S. Lukarevskiy ◽  
S. V. Naidenko ◽  
J. A. Hernandes-Blanko ◽  
...  
Keyword(s):  
Population Size ◽  
Molecular Genetic ◽  
Population Size Estimation ◽  
Individual Identification ◽  
Panthera Pardus ◽  
Size Estimation ◽  
Panthera Pardus Orientalis

scholarly journals Forensic Analysis in Wildlife Crime Cases: Microscopy, DNA Profiling and Isotope Analysis

2021 ◽  
Author(s):  
Vivek Sahajpal ◽  
Sudhanshu Mishra ◽  
Deepika Bhandari
Keyword(s):  
Geographical Origin ◽  
Isotope Analysis ◽  
Forensic Analysis ◽  
Dna Profiling ◽  
Individual Identification ◽  
Wildlife Trade ◽  
Illegal Trade ◽  
Wildlife Crime ◽  
Illegal Wildlife Trade ◽  
Wildlife Forensics

Illegal wildlife trade is one of the biggest threats to the environment and biodiversity. The growing volume of illegal trade in wildlife jeopardizes all the conservation efforts across the globe. Many species have become extinct due to the illegal wildlife trade and many have reached the verge of extinction. According to some estimates, the monetary values of the illegal wildlife trade are estimated to be several billion US dollars. To deal with wildlife crime cases, it becomes imperative to have a sound knowledge of the techniques required in the analysis of wildlife crime exhibits. In this chapter, we have outlined the three frequently used techniques in wildlife forensics viz. microscopy, DNA and isotope analysis for addressing the problems of species and individual identification, and additionally identification of the geographical origin of a wildlife sample. The basic essentials of these techniques have been discussed in this chapter.

scholarly journals Analysis of clinical and molecular genetic characteristics of Wiskott-Aldrich syndrome and X-linked thrombocytopenia

2021 ◽  
Vol 2 (3) ◽  
pp. 61-66
Author(s):  
Doina TURCAN ◽  
Lucia ANDRIES ◽  
Alexandr DORIF ◽  
Victoria SACARA
Keyword(s):  
Actin Polymerization ◽  
Wide Spectrum ◽  
Molecular Genetic ◽  
Clinical Findings ◽  
Recurrent Infections ◽  
Genetic Characteristics ◽  
Wiskott Aldrich Syndrome ◽  
Platelet Size ◽  
First Case ◽  
Small Platelet

Introduction. Wiskott-Aldrich syndrome is a rare X-linked disorder characterized by microthrombocytopenia, eczema, and recurrent infections. It is caused by mutations of the WAS gene which encodes the WAS protein (WASp) – a key regulator of actin polymerization in hematopoietic cells. Mutations within the WASp gene result in a wide heterogeneity of clinical disease, ranging from ‘classical WAS’ to mild asymptomatic thrombocytopenia (X-linked thrombocytopenia [XLT]), or congenital neutropenia (X-lined neutropenia [XLN]).Case presentation. This present paper reports a phenotypical and laboratory description of two children diagnosed with WAS and one child diagnosed with XLT. The first case was a six months old male with septicemia, thrombocytopenia, eczema and petechial rash. The second case was a 2 years old boy presenting with complaints of recurrent infections, eczema and thrombocytopenia with small platelet size. The third case was a 16 years old boy who presented with thrombocytopenia and recurrent sinopulmonary infections.Conclusions. Due to a wide spectrum of clinical findings, the diagnosis of WAS/XLT should be considered in any male patient presenting with petechiae, bruises, and congenital or early-onset thrombocytopenia associated with small platelet size.

scholarly journals DNA-validated parthenogenesis: first case in a captive female Cuban boa (Chilabothrus angulifer)

2019 ◽  
Author(s):  
Fernanda Seixas ◽  
Francisco Morinha ◽  
Claudia Luis ◽  
Nuno Alvura ◽  
Maria dos Anjos Pires
Keyword(s):  
Genetic Analysis ◽  
Captive Breeding ◽  
Biological Process ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
The Other ◽  
First Case ◽  
In Captivity ◽  
Facultative Parthenogenesis ◽  
Parthenogenetic Reproduction

AbstractParthenogenesis is a biological process of asexual reproduction. Recent studies have highlighted the significance of this fascinating phenomenon in the vertebrate evolution. Although parthenogenetic reproduction appears to be widespread among reptiles, a restricted number of cases were reported in captivity and wild. Here, we studied and reported an intriguing case of a 20-year old captive female Cuban boa (Chilabothrus angulifer), from the Zoo da Maia (Maia, Portugal) collection, isolated from conspecifics males, that gave birth twice in 4 years. The neonates from both deliveries, one fresh and the other fixed in formalin, were submitted to histopathological and molecular genetic analysis. Both neonates were homozygous for the loci analyzed, carrying only mother alleles. Furthermore, morphological abnormalities (anophthalmia) were observed in the second neonate. Our data support a pattern of parthenogenetic reproduction. This is the first documented case of facultative parthenogenesis in a Cuban boa, which can be of great interest for further research on ecology, evolution, captive breeding and conservation of the species.

scholarly journals Targeted therapy of hemispheric infant gliomas

2021 ◽  
Vol 20 (3) ◽  
pp. 68-73
Author(s):  
L. I. Papusha ◽  
E. A. Salnikova ◽  
A. V. Panferova ◽  
М. А. Zaytseva ◽  
I. G. Vilesova ◽  
...  
Keyword(s):  
Targeted Therapy ◽  
Tyrosine Kinases ◽  
Kinase Inhibitor ◽  
Molecular Genetic ◽  
Complete Response ◽  
Response To Therapy ◽  
Duration Of Treatment ◽  
Pediatric Hematology ◽  
National Medical ◽  
First Case

Infant high grade gliomas (HGG) are the most frequent tumors of the central nervous system in children below 1 year of age. Standard therapy involves surgical resection and chemotherapy. Prognosis in infant HGG is better than in older patients, however, the absence of effective regimens of anti-recurrence therapy and the impossibility of radiation therapy implementation predetermine a negative prognosis in the group of infant gliomas in case of disease progression. In most patients with infant HGG of hemispheric localization, gene rearrangements of receptor tyrosine kinases – NTRK1/2/3 (24%), ALK (41%), ROS1 (28%), MET (7%) are described. The results of tyrosine kinase inhibitor administration show high efficacy and safety in the treatment of patients with NTRK-positive gliomas. The study was approved by the Independent Ethics Committee of the Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology. The paper presents two examples of the successful use of targeted therapy in patients with infant HGG lacking the efficacy of the standard chemotherapy. In both cases, a persistent response was obtained: in the first case, a complete response to therapy was achieved, the duration of treatment is currently 1 year, in the second case – there is no progression of the disease during 20 weeks of therapy. Of the adverse events (AE) of targeted therapy in patients, only transitory neutropenia was noted in the first case, in the second case, AEs were not detected. In order to expand therapeutic options and prescribe targeted therapy drugs, a molecular genetic investigation of tumor tissue is mandatory for patients with hemispheric infant gliomas. The parents of the patients agreed to use the information, including photos of children, in scientific research and publications.

scholarly journals Primary Episcleral Melanoma Consistent with Uveal Melanoma Mutations Treated by Excision and Gamma Knife Stereotactic Radiosurgery

2019 ◽  
Vol 6 (2) ◽  
pp. 93-98
Author(s):  
Spencer T. Langevin ◽  
Eileen Chang ◽  
Tony J.C. Wang ◽  
Israel Deutsch ◽  
Marc Otten ◽  
...  
Keyword(s):  
Gamma Knife ◽  
Uveal Melanoma ◽  
Molecular Genetic ◽  
Complete Excision ◽  
First Case ◽  
Increased Risk ◽  
Orbital Tissue ◽  
Residual Tissue ◽  
Gamma Knife Stereotactic Radiosurgery ◽  
Ocular Melanocytosis

Ocular melanocytosis has traditionally been associated with increased risk of developing uveal melanoma; however, rarely primary episcleral melanoma has been reported in the literature. Herein, we present the third case of primary episcleral melanoma treated by complete excision and cryotherapy. In contrast to previous cases, we obtained molecular genetic testing which revealed a GNA-11 mutation, and gene expression profiling resulted in a Class 2 PRAME positive tumor diagnosis. These two tests which have never been performed on previous cases, support lineage similar to uveal melanoma. In addition, we are the first case to report treatment of the surgical bed and surrounding orbital tissue with Gamma Knife stereotactic radiotherapy to both treat residual tissue and decrease risk of recurrence.

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