scholarly journals Bull semen quality parameters and impact of exogenous factors on semen quality

2014 ◽  
Vol 68 (3-4) ◽  
pp. 229-239
Author(s):  
Tomaz Snoj
Keyword(s):  
Semen Quality ◽  
Geographic Area ◽  
Quality Parameters ◽  
Semen Parameters ◽  
Winter Period ◽  
Pesticide Use ◽  
Eu Countries ◽  
Bull Semen ◽  
Exogenous Factors ◽  
Sperm Output

Use of quality sires in the artificial insemination of cattle leads to the increased production in cattle breeding. In EU countries and the countries which cooperate in semen trade with EU countries bulls? health status and minimal criteria for semen quality are regulated by law. Bull semen quality is influenced by several exogenous factors. Semen quality parameters are influenced by season. The highest values of ejaculate volume and total sperm output were found during the summer, while the lowest values were found in winter period. Some xenobiotics can also affect semen parameters. Correlation between semen quality and pesticide use in limited geographic area was found, namely bull semen quality decreased in the period when pesticide use increased.

scholarly journals Effect of Supplementation of Polyvinylpyrrolidone in Extender on Buffalo Semen Parameters

2020 ◽  
Vol 53 (1) ◽  
Author(s):  
Bushra Ismail Khan ◽  
Shamim Akhter ◽  
Sanwal Aslam ◽  
Rabea Ejaz
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Bubalus Bubalis ◽  
Semen Parameters ◽  
Suction Pump ◽  
Bull Semen ◽  
Buffalo Semen ◽  
Live Sperm ◽  
And Control

The current study was planned to evaluate the supplementation of Polyvinylpyrrolidone in extender on cryopreservation of Nili-Ravi buffalo bull semen. The semen samples were collected from Nili-Ravi buffalo (Bubalus bubalis) bull kept at SPU Qadirabad, District Sahiwal, Pakistan. Qualifying semen ejaculates having motility >60%, volume >5-6ml and concentration >0.5 billion/ ml were diluted 50 × 106 motile sperm ml approximately at 37°C in Tris-citric acid extender supplemented with different concentrations of PVP (0.01, 0.05, 0.1mM). The extender without PVP was kept as control. Semen was stored at 4°C for a period of 2 h and kept at 4°C for 4h. Semen was filled in 0.5 ml French straws using suction pump at 4°C, plunged and stored in liquid nitrogen (-196°C). Semen straws were rewarmed at 37°C for 30 seconds and assessed for sperm motility, plasma membrane integrity (PMI), dead sperm percentage and the live sperm percentage. The data on the role of PVP on different parameters of semen quality were analyzed by using ANOVA and RCBD. Higher percentage (P< 0.05) of sperm motility (66.1±7.51 and 59.4±10.72) and PMI (72.9±5.39 and 75.7±6.5) was observed in extenders having 0.05 mM and 0.1mM PVP compared to extenders having 1.5mM PVP and control. The percentage acrosomal integrity was observed greater (P< 0.05) in extended semen containing 0.1mM (68.2±0.50) PVP compared to extenders having 0.01 and control.

scholarly journals Evaluating bull fertility based on non-return method

2012 ◽  
Vol 66 (1-2) ◽  
pp. 27-40
Author(s):  
Igor Prka ◽  
Dragan Vukovic ◽  
Stevan Perkovic
Keyword(s):  
Semen Quality ◽  
Sperm Quality ◽  
Quality Parameters ◽  
Semen Parameters ◽  
Service Period ◽  
Microscopic Evaluation ◽  
Live Sperm ◽  
Return Method ◽  
The Impact ◽  
First Time

In order to evaluate the results of reproductive cows and heifers, different parameters of fertility are used, such as the service period, insemination index, intercalving time and others, and of the breeding bulls the values obtained through non-return. An ejaculate is taken up for further processing by veterinary centres only provided it meets the prescribed quality parameters. Rating semen parameters includes a macroscopic (volume, colour, consistency, smell and pH) and a microscopic evaluation (mobility, density, percentage of live sperm and abnormal and damaged sperm). In addition to sperm quality and the fertility of the female animal, the results of the non-return method are also influenced by a number of exogenous causes (season, age, race, insemination techniques) that have no small impact on the end result of insemination - pregnancy. In order to obtain more objective results of the fertility of bulls the following tasks were undertaken, namely: 1. to calculate with the non-return method the fertility of bulls in over 10,000 cows inseminated for the first time during a period of 6 years; and 2. to analyze the impact of semen quality, season, age of cow and bull, and the bull breed on the results of fertility.

scholarly journals Effect of Age and Frequency of Collection on Quality of Jersey Bulls Semen at National Livestock Breeding Center (NLBC), Nepal

2019 ◽  
Vol 7 (1) ◽  
pp. 88-95 ◽  
Author(s):  
S. Sankhi ◽  
K.R. Sapkota ◽  
B. Regmi
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Bull Semen ◽  
Livestock Breeding ◽  
Abnormal Sperm ◽  
Breeding Center ◽  
Live Sperm ◽  
Level Of Significance ◽  
Effect Of Age

Objective: To determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara. Materials and Methodology: Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3-4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan, 2016). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0 Results: Sperm concentration, post- thaw motility, live sperm, and abnormal sperm were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. Significantly higher value for semen volume, sperm concentration and post- thaw motility was obtained on second collection (P<0.05). However, volume initial motility, pre-filling motility, live sperm and abnormal sperm percentage did not differ significantly among different age intervals and between the frequencies of collection. Conclusion: Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentages were higher in 5-7 years old bulls and in second collection of semen. Int. J. Appl. Sci. Biotechnol. Vol 7(1): 88-95

scholarly journals Effect of Age and Frequency of Collection in Quality of Jersey Bulls Semen at National Livestock Breeding Center, Nepal

2018 ◽  
Vol 35 ◽  
pp. 9-22
Author(s):  
S. Sankhi ◽  
K.R. Sapkota ◽  
B. Regmi
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Bull Semen ◽  
Livestock Breeding ◽  
Abnormal Sperm ◽  
Breeding Center ◽  
Live Sperm ◽  
Level Of Significance ◽  
Effect Of Age

The present study was undertaken to determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara, Kaski district. Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3- 4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan 2017). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0. Sperm concentration (0.551 ±0.004 to 1.084 ± 0.014x109), post- thaw motility (42.44±0.11 to 51.72±0.03 %), live sperm (45.62 ± 0.13 to 51.23±0.15 %), and abnormal sperm (11.57±0.12 to 19.59±0.24%) were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. On the other hand, volume (3.98±0.09 to 6.72±0.06 ml), initial motility (72.68±0.02 to 78.64±0.15 %) and pre-filling motility (71.34±0.17 to 77.62±0.02 %) did not differ significantly among different age intervals. Significantly, higher value for semen volume (3.98±0.12 to 6.02±0.14 ml), sperm concentration (0.572±0.119 to 1.095±0.015) and post- thaw motility (40.34±0.21 to 48.54±0.06% was obtained on second collection. However, initial motility (70.58±0.06 to 75.64±0.13 %), prefilling motility (68.11±0.12 to 71.62±0.02 %), live sperm (53.27±0.54 to 56.17±0.02 %) and abnormal sperm (12.31±0.12 to 14.3±0.47 %) did not differ significantly between the frequencies of collection. Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentage were higher in 5-7 years old bulls and in second collection of semen.

scholarly journals Manganese Provides Antioxidant Protection for Sperm Cryopreservation that May Offer New Consideration for Clinical Fertility

2009 ◽  
Vol 2 (3) ◽  
pp. 152-159 ◽  
Author(s):  
Ranjna S. Cheema ◽  
Amrit K. Bansal ◽  
Gurmail Singh Bilaspuri
Keyword(s):  
Protective Effect ◽  
Semen Quality ◽  
Maximum Level ◽  
Egg Yolk ◽  
Quality Parameters ◽  
Vitro Fertilization ◽  
Bull Semen ◽  
Protein Leakage ◽  
A Chain

Reactive oxygen species (ROS) are generated by sperm metabolism. While, ROS are required for maturation, capacitation and acrosome reaction, they also modify many peroxidable cellular compounds. There is production of ROS during cryopreservation and frozen spermatozoa are highly sensitive to lipid peroxidation (LPO). Antioxidants exert a protective effect on the plasma membrane of frozen bovine sperm preserving both metabolic activity and cellular viability. Manganese (Mn++) is proved to be a chain breaking antioxidant in biological system. Therefore, we examined the role of (Mn++) during cryopreservation of cattle bull semen. Semen was divided into four parts and cryopreserved in egg-yolk-citrate extender + glycerol (EYC-G), EYC-G + 100 µM of Mn++, EYC-G + 150 µM of Mn++and EYC-G + 200 µM of Mn++. After four hours of cooling and 24 hrs of freezing, the spermatozoa were examined for percentage motility, Hypo-osmotic swelling (HOS), LPO and protein leakage. Addition of manganese to the semen during cryopreservation showed a protective effect and accounted for an increase in semen quality parameters [percentage motility, HOS percent and decrease in malondialdehyde (MDA) production and protein leakage]. The effect of manganese on motility and HOS was non-significant (p < 0.05) in cooled spermatozoa but significant with 150 µM of Mn++in frozen-thawed spermatozoa. MDA production and protein leakage decreased to a significant and maximum level (p < 0.05) on addition of 200 µM of manganese. The addition of manganese to EYC-G dilutor will improve the quality/fertility of semen, which will result in improvement of in vitro fertilization and artificial insemination success rate.

scholarly journals Effect of whole and clarified egg yolk based extenders on post-thaw semen quality in bulls.

2021 ◽  
Author(s):  
Prosper Kamusasa ◽  
Eddington Gororo ◽  
Fungayi Primrose Chatiza
Keyword(s):  
Semen Quality ◽  
Sperm Quality ◽  
Egg Yolk ◽  
Quality Parameters ◽  
Normal Morphology ◽  
Semen Cryopreservation ◽  
Bull Semen ◽  
Inclusion Level ◽  
Whole Egg

Abstract This study was conducted to evaluate the comparative cryoprotective effects of whole egg yolk and clarified egg yolk on post thaw sperm quality parameters and to determine the optimum clarified egg yolk inclusion level (10-20%) in semen extenders for Mashona bull semen cryopreservation. It was shown that there was a significant decrease in sperm quality variables following cryopreservation. Semen quality increased with the concentration of clarified egg yolk, indicating a positive relationship between egg yolk LDL concentration and maintenance of in vitro sperm quality. The 20% clarified egg yolk (CEY20) extender treatment gave post-thaw motility, viability and normal morphology values which were comparable to the control (20% whole egg yolk, WEY20). The 10% clarified egg yolk concentration gave the least post-thaw quality values and the greatest proportion of defective spermatozoa. This experiment found no advantage of replacing whole egg yolk with up to 15% clarified egg yolk in Mashona bull semen cryopreservation. However, 20% clarified and 20% whole egg yolk performed similarly in the maintenance of post-thaw sperm motility, viability and normal morphology.

scholarly journals  Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls

2012 ◽  
Vol 57 (No. 10) ◽  
pp. 490-497 ◽  
Author(s):  
P. Padrik ◽  
T. Hallap ◽  
T. Kaart ◽  
T. Bulitko ◽  
Ü. Jaakma
Keyword(s):  
Quality Assessment ◽  
Sperm Motility ◽  
Semen Quality ◽  
Age Groups ◽  
Quality Parameters ◽  
Laboratory Method ◽  
Osmotic Swelling ◽  
Bull Semen ◽  
Sperm Membrane ◽  
Dairy Bulls

&nbsp;As an attempt to find an inexpensive and simple laboratory method for artificial insemination (AI) bull semen quality assessment, the osmotic resistance of spermatozoa was measured using the hypo-osmotic swelling (HOS) test, developed by Jeyendran et al. (1984) (labelled HOS-1), and its modifications (HOS-2, HOS-3), with decreased osmotic pressure aimed at challenging sperm survival ability. The test results were benchmarked against sperm viability measurements performed using the Computerized Motility Analyzer (CMA), and field fertility was calculated as non-return rate (NRR). Two age groups of Estonian Holstein bull sires were included in this study to test possible age effect on semen quality parameters. The HOS-1 test in fresh bull semen correlated well with sperm general motility (GMot) (r = 0.63, P &lt; 0.001 at batch level and r = 0.77, P &lt; 0.001 at bull level) as well as with progressive motility (PMot) in frozen-thawed (FT) semen (r = 66, P &lt; 0.001 at batch level and r = 0.81, P &lt; 0.001 at bull level), which makes the test suitable for the prediction of post-thaw semen quality. However, the HOS-2 and HOS-3 values in FT semen had high correlations with NRR (r = 0.65, r = 0.66, P &lt; 0.001 at batch level and r = 0.63, r = 0.71, P &lt; 0.01 at bull level), which was comparable to those between GMot and NRR or PMot and NRR. A combination of motility parameters and the results of the HOS-1 and HOS-3 tests provided a good model for predicting the potential fertility of bull semen. Values of sperm membrane post-thaw intactness, assessed using HOS-2, as well as of sperm motility measurements were higher in mature bulls compared to those in young bulls. Short conclusion: different modifications of the hypo-osmotic swelling test are useful for routine bovine semen quality assessment at AI stations. &nbsp;

scholarly journals Higher adherence to Mediterranean Diet is associated with improved semen parameters: A systematic review and meta-analysis of observational studies

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Jakub Morze ◽  
Anna Danielewicz ◽  
Katarzyna Przybylowicz
Keyword(s):  
Mediterranean Diet ◽  
Observational Studies ◽  
Semen Quality ◽  
Sperm Count ◽  
Meta Analysis ◽  
Quality Parameters ◽  
Semen Parameters ◽  
Eligibility Criteria ◽  
Cross Sectional ◽  
Mean Differences

AbstractSemen quality is a key indicator of male fecundity and therefore is closely related to male fertility. In recent decades a significant decrease of semen parameters was observed worldwide. Except for environmental pollution, low physical activity, body weight and comorbidities, unhealthy diet was linked to semen quality decline. Mediterranean Diet (MedDiet) due to its anti-oxidative and anti-inflammatory properties, is considered as beneficial for semen. However, results of existing studies are inconclusive. The aim of this review was to pool associations between adherence to the MedDiet and semen quality parameters in observational studies. Electronic databases including PubMed, Embase and Scopus were searched until April 2019 with no restriction to language or time. Studies were eligible for inclusion if they filled following criteria: (1) observational design (retrospective or prospective), (2) adherence to MedDiet assessed using score or data-driven method and (3) semen quality parameters (total motility, progressive motility, total count, concentration or volume) as outcomes. Mean differences or odds ratios of abnormal semen parameters between the highest and lowest quintiles of MedDiet were converted to standardized mean differences (SMD). Random effects models with inverse variance method were used to pool differences. Heterogeneity was assessed using the Cochrane Q test and I2 statistic with value > 50% indicating substantial heterogeneity. Five cross-sectional studies extracted from 840 articles, met the eligibility criteria and were included in the meta-analysis. Higher adherence to MedDiet compared to lower adherence was associated with increased total (SMD = 0.59 95%CI: 0.12–1.06, I2 = 80%, pheterogenity < 0.01, n = 4) and progressive (SMD = 0.53 95%CI: 0.16–0.91, I2 = 0%, pheterogenity = 0.94, n = 2) sperm motility. No associations were observed between adherence to MedDiet and total sperm count (SMD = 0.14 95%CI: -0.20–0.48, I2 = 70%, pheterogenity < 0.01, n = 5), concentration (SMD = 0.22 95%CI: -0.04–0.47, I2 = 48%, pheterogenity = 0.10, n = 5), volume (SMD = 0.02 95%CI: -0.20–0.25, I2 = 21%, pheterogenity = 0.28, n = 5), as well as morphology (SMD = -0.10 95%CI: -0.36–0.16, I2 = 38%, pheterogenity = 0.18, n = 4). The results suggest that higher adherence to the MedDiet may have a protective effect on semen quality, probably by increased intake of antioxidant-rich products. Limitations of findings include substantial between-study heterogeneity and use of cross-sectional design. Beneficial effects of the MedDiet on semen quality have to be confirmed in further prospective studies.

Retrospective study of bull semen quality — possible correlation with pesticide use?

2013 ◽  
Vol 61 (4) ◽  
pp. 495-504
Author(s):  
Tomaz Snoj ◽  
Silvestra Kobal ◽  
Blanka Premrov Bajuk ◽  
Monika Zuzek ◽  
Nina Cebulj-Kadunc ◽  
...  
Keyword(s):  
Semen Quality ◽  
Linear Regression Analysis ◽  
Geographical Area ◽  
Environmental Chemicals ◽  
Pesticide Use ◽  
Endogenous Hormones ◽  
Upward Trend ◽  
Bull Semen ◽  
The Given

Decline in semen quality in humans and increased incidence of male reproductive problems could be caused by different factors, including pesticides that could mimic or block the action of endogenous hormones. If the decline in semen quality is real, and environmental chemicals are at least partially responsible for this decline, similar changes should be observed in animals that live in close connection with humans and are exposed to similar levels of pollutants. In the present study, the semen quality of bulls in the last 30 years was examined with respect to the year of birth. Furthermore, semen quality results were correlated to the total pesticide use in a limited geographical area. The results indicate a notable decrease in both ejaculate volume and total number of spermatozoa in ejaculates of bulls born in the late 1970s, while after that (until 2006) there was no obvious downward or upward trend either in ejaculate volume or in the total number of sperm cells. The amount of pesticides released into the environment increased about twofold in the given period, and linear regression analysis revealed a strong and statistically significant correlation between the amount of pesticides used and the total number of spermatozoa in the ejaculate.

scholarly journals The Influence of Zinc and Heavy Metals in Feed and Water on the Quality of Cryopreserved Bull Semen

2021 ◽  
Vol 71 (3) ◽  
pp. 326-343
Author(s):  
Goran Jakovljević ◽  
Miodrag Lazarević ◽  
Milorad Mirilović ◽  
Aleksandar Milovanović ◽  
Jelena Apić ◽  
...  
Keyword(s):  
Semen Quality ◽  
National Research Council ◽  
Quality Parameters ◽  
Computer Assisted ◽  
Sperm Cells ◽  
Strong Negative Correlation ◽  
Sperm Analysis ◽  
Bull Semen ◽  
Group B ◽  
Group D

Abstract This study aimed to assess the influence of different concentrations of zinc (Zn), lead (Pb), mercury (Hg) and cadmium (Cd) found in the feed and water provided to bulls on the concentrations of these metals in cryopreserved bull semen, and to determine their influence onto semen quality parameters. Correlations between heavy metal concentrations in the semen and the quality parameters of semen as estimated by computer-assisted sperm analysis (CASA) and flow cytometry (FC) methods were determined. A total of 40 cryopreserved semen samples originating from bulls housed in 4 different centers for artificial insemination (A, B, C and D) were examined, making a total of 160 samples. The concentrations of metals and semen quality parameters were determined in cryopreserved semen of 10 bulls from each center, namely 4 samples from each bull. Concentrations of Zn, Pb, Hg and Cd in hay and concentrated feed were within the allowed limits as proposed by the National Research Council (NRC, 2000). A strong negative correlation was detected between curvilinear velocity (VCL) and Zn concentrations in the semen (P < 0.01; r = -0.772) in group D, and a positive correlation of VCL with Pb concentrations (P < 0.05 and r = 0.718) in group B. Mercury concentrations in cryopreserved semen correlated negatively to the percent of live sperm cells with intact acrosomes (V/IA: P < 0.05; r = -0.640) and positively with the percent of dead sperm cells with damaged acrosomes (D/DA: P < 0.01; r = 0.766) in group D. This finding confirms the hypothesis that Hg, even at low concentrations, may cause acrosome damage.

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