scholarly journals Emotional Stress- and Pain-Related Behaviors Evoked by Experimental Tooth Movement

2008 ◽  
Vol 78 (3) ◽  
pp. 487-494 ◽  
Author(s):  
Joseph H. Yozgatian ◽  
Jorge L. Zeredo ◽  
Hitoshi Hotokezaka ◽  
Yoshiyuki Koga ◽  
Kazuo Toda ◽  
...  
Keyword(s):  
Open Field ◽  
Emotional Stress ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Animal Activity ◽  
Experimental Group ◽  
Experimental Tooth Movement

Abstract Objective: To investigate by behavioral methods the relationship between emotional stress and pain during experimental tooth movement in rats. Materials and Methods: Sixteen male Sprague-Dawley rats (210 to 250 g) were divided into two groups. The experimental group was treated with an active Ti-Ni appliance, and the control group received a passive appliance. A force of 20 gf was delivered by the active appliance between the maxillary first and second molars for 3 days. During this period the rat's behavior was evaluated eight times by means of open-field test and resistance-to-capture test. The specific parameters of animal activity were facial grooming, rearing, and locomotor activity, movement into the center of the open field, and response to capture. Results: Parameters related to stress and pain were higher in the group carrying active appliance, compared to the group with a passive appliance. Statistically significant differences in stress-related behavior between control and experimental groups were found 8 hours after placing the appliance and were most evident on the second day. Pain-related behavior was significantly greater in the experimental group than in the control group at 24 hours. Conclusions: The increase in emotional stress evoked by orthodontic tooth movement may precede the appearance of periodontal pain.

scholarly journals Combined Effect of Nicotine and Caffeine on Orthodontic Tooth Movement in Rats

2020 ◽  
Vol 9 (2) ◽  
pp. 109-114
Author(s):  
NAEEM MURTAZA ◽  
WAHEED UL HAMID ◽  
AHMAD SHAMIM ◽  
SHABBIR HUSSAIN ◽  
Muhammad Azeem ◽  
...  
Keyword(s):  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Combined Effect ◽  
Medical Institute ◽  
Control Group ◽  
P Value ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
The Mean ◽  
Animal House

Background: The individual effects of nicotine and caffeine have been reported in previous studies but their combined effect on tooth movement needs to be elucidated. The objective of this study was to evaluate the combined effect of nicotine and caffeine on the magnitude of orthodontic tooth movement (OTM) in rats. Material and Methods: This experimental study was conducted on Sprague-Dawley rats (Animal House and Pathology Laboratory; Post Graduate Medical Institute, Lahore) in the department of Orthodontics, de’Montmorency College of Dentistry, Lahore from 8th July 2014 to 8th January 2015. Forty male Sprague-Dawley rats were divided into four equal groups: Control group (CR), nicotine group (NT), caffeine group (CF) and combined nicotine and caffeine group (CNC). Closed coil nickel titanium (NiTi) spring was placed between incisor and maxillary molar. Nicotine group (NT) was treated by intraperitoneal injections of nicotine. Caffeine was given to caffeine group and Combined nicotine and caffeine group (CNC) was treated in the same way as individual nicotine and caffeine groups daily for 14 days. All the rats were sacrificed on 15th day. Magnitude of the orthodontic tooth movement was measured using digital Vernier caliper. Means and standard deviation were calculated for orthodontic tooth movement. One-way ANOVA was used to determine the mean difference in OTM. Post hoc Tukey test was used for multiple comparisons among the groups. Results: The mean orthodontic tooth movement (OTM) was 0.32 mm ± 0.05 in control group, 0.56 mm ± 0.04 in nicotine group, 0.52 mm ± 0.034 in caffeine group and 0.8 mm ± 0.06 in combined NC group, respectively. The difference between mean OTM among the groups was statistically significant (P-value <0.001). The mean OTM in CNC group was significantly higher as compared to other groups (CR, NT, CF, NT) (P-value <0.001). Conclusions: In rats, the combined use of nicotine and caffeine results in greater orthodontic tooth movement as compared to their individual use.

scholarly journals Effects of Hyperbaric Oxygen during Experimental Tooth Movement

2008 ◽  
Vol 78 (2) ◽  
pp. 304-308 ◽  
Author(s):  
Sila Gokce ◽  
A. Osman Bengi ◽  
Erol Akin ◽  
Seniz Karacay ◽  
Deniz Sagdic ◽  
...  
Keyword(s):  
Trabecular Bone ◽  
Hyperbaric Oxygen ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Sprague Dawley ◽  
Bone Apposition ◽  
Male Adult ◽  
Osteoblastic Activity ◽  
Sprague Dawley Rats ◽  
Experimental Tooth Movement

Abstract Objective: To determine the effects of hyperbaric oxygen (HBO) on bone remodeling during orthodontic tooth movement. Materials and Methods: Twenty-four male, adult Sprague Dawley rats were randomly divided into two groups. HBO was administered in the first group, and the second group served as a control. The mandibular first molars were moved mesially by means of Ni-Ti closed coil springs in all groups. Results: Results were evaluated histomorphometrically and the parameters of trabecular bone volume (BV/TV), trabecular bone number (Tr.N), and trabecular separation (Tr.Sep) were evaluated at the interradicular bone area of the mandibular first molars. Increases in BV/TV and Tr.N and decreases in Tr.Sep revealed the osteoblastic activity of HBO. HBO application caused an increase in bone apposition and osteoblastic activity or a decrease in osteoclastic activity. Conclusions: HBO enhanced the bone formation during experimental tooth movement. Therefore, the findings of this study support our hypothesis that osteoblastic activity might be modulated by changes in the environmental oxygen tension.

scholarly journals Ameliorative effects of Spinacia oleracea on sperm morphology, count, and motility by normalizing the obesity-induced oxidative stress in Sprague Dawley rats

2020 ◽  
Vol 14 (03) ◽  
pp. 124-127
Author(s):  
Somia Iqbal ◽  
Noman Sadiq ◽  
Saad Siddiqui ◽  
Hira Iqbal
Keyword(s):  
Sperm Concentration ◽  
Treated Group ◽  
Sperm Parameters ◽  
Control Group ◽  
Sprague Dawley ◽  
Normal Morphology ◽  
Sprague Dawley Rats ◽  
Group A ◽  
Group B ◽  
Experimental Group

Background: Obesity is a prevailing metabolic disorder that affects the functioning of the male reproductive system. Excessive adipose tissue enhances reactive oxygen species generation and is linked with male infertility. Spinach has demonstrated antioxidant effects. The present study was conducted to determine the antioxidant effects of spinach on sperm parameters in obese Sprague Dawley rats. Subjects and methods: This randomized control study was conducted at the animal house of the National Institute of Health Islamabad, Islamic International Medical College, Cosmesurge International Hospital, Rawalpindi, and Apollo lab, Islamabad, Pakistan from April 2016 to March 2017. Forty male Sprague Dawley rats having an age of 8 weeks and weight 160-200g were tagged from number 1 to 40. Every third rat was randomly allocated to control Group A (n=13) and remaining into the Experimental group (n=27). Rats of control Group A was given a standard diet while a high-fat diet was given to Experimental group rats to induce obesity for the duration of six weeks. Weight (g) was measured weekly and obesity was confirmed when rats attain more than 20% weight when compared with that of rats of control Group A. Then, after obesity induction, the experimental group was alienated into the obesity control group (Group B) and spinach treated group (Group C). For sample, rats of Group A and Group B were sacrificed, and the cauda epididymis of each rat was placed in a Petri dish containing normal saline and cut into pieces to allow the release of sperm and then sperm parameters (sperms concentration, motility, and morphology) were recorded under the microscope. Then, spinach (5% hot water extract) along with the persistence of fat diet was administered to Group C for 4 weeks and finally, sperm parameters were measured in this group. Results: Sperm concentration/ml, motility (%), and normal morphology (%) of Group B rats were significantly decreased as compared to Group A rats. However, sperm concentration/ml, motility (%), and normal morphology (%) of Group C (spinach treated group) rats was significantly increased (p<0.001) as compared to Group B (obesity control group) rats after administering spinach. Conclusion: The addition of Spinach in a normal diet regimen restores normal sperm morphology, improves sperm motility and concentration.

The cartilage degeneration in a growing rat experimental model of developmental trochlear dysplasia is associated with activation of PI3K/AKT signal pathway

2020 ◽  
Author(s):  
Wei Lin ◽  
Yike Dai ◽  
Jinghui Niu ◽  
Chongyi Fan ◽  
Xunkai Feng ◽  
...  
Keyword(s):  
Trochlear Dysplasia ◽  
Cartilage Degeneration ◽  
Signal Pathway ◽  
Control Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Growing Rat ◽  
Polymerase Chain ◽  
Underlying Mechanisms ◽  
Experimental Group

Abstract Background As one of the lower extremity deformities in human, trochlear dysplasia is a commonly encountered disease. However, the molecular mechanism of cartilage degeneration in trochlear dysplasia is indefinite yet. It was apparent to all that PI3K/AKT signal pathway is extremely significant in regulating the pathophysiological process of cartilage degeneration. The purpose of this research is to discuss the correlation between PI3K/AKT signal pathway and trochlear dysplasia cartilage degeneration. Materials and methods 120 female Sprague-Dawley rats at 4 weeks of age were separate into control group and experimental group randomly. The distal femurs were isolated from the experimental and unsurgeried control group at the point of the 4, 8, 12 weeks, correspondingly. Micro-CT and histological examination were carried out to investigate the anatomical structure and cartilage changes of the trochlear. Subsequently, the expression of PI3K/AKT, TGFβ1 and ADAMTS-4 in cartilage were investigated by immunohistochemistry and quantitative real-time polymerase chain reaction (qRT-PCR). Results In the experimental group, the trochlear dysplasia model was successfully established at 8 weeks after surgery. Moreover, the cartilage degeneration was found from 8 weeks, with continued higher protein and mRNA expression of PI3K/AKT, TGFβ1 and ADAMTS-4 compared with the control group. Conclusions This research suggested that patellar instability may lead to trochlear dysplasia in growing rats. Moreover, trochlear dysplasia was probably one of the causes of patellofemoral osteoarthritis and the cartilage degeneration in trochlear dysplasia might be associate with activation of PI3K/AKT signal pathway. However, more research was required to clarify the underlying mechanisms.

scholarly journals The Administration of 4-Hexylresorcinol Accelerates Orthodontic Tooth Movement and Increases the Expression Level of Bone Turnover Markers in Ovariectomized Rats

2020 ◽  
Vol 21 (4) ◽  
pp. 1526 ◽  
Author(s):  
Kwang-Hyo Choi ◽  
Dae-Won Kim ◽  
Suk Keun Lee ◽  
Seong-Gon Kim ◽  
Tae-Woo Kim
Keyword(s):  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Ovariectomized Rats ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase ◽  
Sprague Dawley ◽  
Osteogenic Markers ◽  
Surgical Methods ◽  
Elevated Expression ◽  
Turnover Markers

Surgical methods for accelerating orthodontic tooth movement are limited by possible damage to the tooth root and patient discomfort. 4-Hexylresorcinol (4HR) has been shown to increase bone remodeling and may potentially facilitate tooth movement. This study investigated the (1) effect of 4HR administration on osteoblast-like cells and (2) effect of 4HR administration on tooth movement in ovariectomized rats. Saos-2 cells were treated with either 4HR or solvent (control). Protein expression levels were investigated 2, 8, and 24 h after treatment. Thirty ovariectomized Sprague-Dawley rats were divided into two experimental groups (A and B) and one control group. After installation of an orthodontic tooth movement device, groups A and B received subcutaneous weekly injections of 4HR (1.28 and 128 mg/kg). Micro-computerized tomography and histological analyses were performed after 2 weeks of tooth movement. The application of 4HR elevated expression of osteogenic markers in Saos-2 cells. Movement of the first molars was significantly greater in rats administered 4HR. Furthermore, the expression of bone morphogenic protein-2, receptor activator of nuclear factor kappa-B ligand, osteocalcin, and tartrate-resistant acid phosphatase were increased after 4HR administration. 4HR application demonstrated increased expression of osteogenic markers in Saos-2 cells and accelerated orthodontic tooth movement in rats.

scholarly journals Effects of Interferon-Gamma on Bone Remodeling during Experimental Tooth Movement

2007 ◽  
Vol 77 (1) ◽  
pp. 135-141 ◽  
Author(s):  
Sila Mermut ◽  
Ali Osman Bengi ◽  
Erol Akin ◽  
Mehmet Kürkçü ◽  
Şeniz Karaçay
Keyword(s):  
Trabecular Bone ◽  
Bone Remodeling ◽  
Interferon Gamma ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Bone Area ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Ifn Γ ◽  
Different Doses

Abstract Objective: To determine the effects of interferon-gamma (IFN-γ) on bone remodeling during orthodontic tooth movement. Materials and Methods: Thirty adult male Sprague Dawley rats were randomly categorized into five groups. IFN-γ was administered in three different doses (0.01, 0.02, and 0.05 μg/20 μL) and the remaining two groups served as control. Mandibular first molars were moved mesially by means of Ni-Ti closed coil springs in all groups. The results were evaluated histomorphometrically, and parameters of trabecular bone volume (BV/TV), trabecular bone number (Tr.N), and trabecular separation (Tr.Sep) were observed at the interradicular bone area of the mandibular first molars. Results: Increases in BV/TV and Tr.N and decreases in Tr.Sep revealed the antiosteoclastic activity of IFN-γ. Conclusion: IFN-γ administration may be useful clinically for anchorage control.

Effect of neonatal capsaicin treatment on orthodontic tooth movement in male Sprague-Dawley rats

2011 ◽  
Vol 139 (4) ◽  
pp. e345-e352 ◽  
Author(s):  
Julian E. Davila ◽  
James R. Miller ◽  
James S. Hodges ◽  
John P. Beyer ◽  
Brent E. Larson
Keyword(s):  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals IMPACT OF ZOLEDRONATE BISPHOSPHONATE GEL IN VIRGIN COCONUT OIL ON THE INCREASE OF OSTEOCLAST APOPTOSIS

2018 ◽  
Vol 9 ◽  
pp. 24
Author(s):  
Carolin Parlina ◽  
Erni H Purwaningsih ◽  
Ahmad Aulia Jusuf ◽  
Retno Widayati
Keyword(s):  
Buccal Mucosa ◽  
Caspase 3 ◽  
Coconut Oil ◽  
Control Group ◽  
Sprague Dawley ◽  
Emulsion Gel ◽  
Sprague Dawley Rats ◽  
The Impact ◽  
Experimental Group ◽  
Osteoclast Apoptosis

Objective: This study aimed to show the impact of the ZOL in VCO gel (Ge-ZOL) on the extent of osteoclasts apoptosis.Methods: The study used 27 Sprague-Dawley rats which were divided into three groups: Nine rats in the experimental group were given 40 µg of Ge-ZOL, nine rats in the control group were given VCO emulsion gel without ZOL (Ge-), and nine rats in the normal group were not given any treatment. The gel was applied to the buccal mucosa using a cotton bud for 2 min at hour of 0, 4, and 8 on days 0, 1, 2, 3, and 4. The rats were sacrificed on days 1, 3, and 5, and then, evaluated by immunohistochemical caspase-3 staining.Result: The number of apoptotic osteoclast cells in the experimental group was significantly higher than in the control and normal groups (p<0.05). The number of apoptotic osteoclast cells in the experimental group on the day 1 was significantly higher than on the days 3 and 5 (p<0.001).Conclusion: The application of Ge-ZOL to the buccal mucosa proven to improve the number of apoptotic osteoclast cells in the experimental group on the day 1, and this number was higher than on the days 3 and 5.

On Effects of Paternal Ethanol Treatment on Fetal Outcome

1985 ◽  
Vol 57 (1) ◽  
pp. 51-57 ◽  
Author(s):  
H. Cake ◽  
I. Lenzer
Keyword(s):  
Litter Size ◽  
Fetal Outcome ◽  
Ethanol Administration ◽  
Control Group ◽  
Ethanol Treatment ◽  
Sprague Dawley ◽  
Water Control ◽  
Sprague Dawley Rats ◽  
Experimental Group ◽  
Low Dosage

To study the effects of paternal chronic low-dosage ethanol administration on fetal outcome, male Sprague-Dawley rats received either .6g/ kg ethanol (experimental group) or water (control group). Males were mated between the fourth and seventh weeks of treatment. Pregnancies were terminated on gestational Day 21. Cerebral weight and placental weight in offspring of the ethanol-treated males were significantly larger than those of control males. There was no effect on litter size. Experimental females (dams that became pregnant when paired with an ethanol-treated male) were significantly heavier than control dams. Since these pairings were random at every mating, the experimental males appeared to have been more successful impregnating heavier than lighter females.

scholarly journals Effects of Gradually Increasing Force Generated by Permanent Rare Earth Magnets for Orthodontic Tooth Movement

2006 ◽  
Vol 76 (6) ◽  
pp. 1004-1009 ◽  
Author(s):  
Ryo Tomizuka ◽  
Hiroyasu Kanetaka ◽  
Yoshinaka Shimizu ◽  
Akihiro Suzuki ◽  
Kaoru Igarashi ◽  
...  
Keyword(s):  
Rare Earth ◽  
Tooth Movement ◽  
Root Resorption ◽  
Orthodontic Tooth Movement ◽  
Male Rats ◽  
Control Group ◽  
Pathological Changes ◽  
Group I ◽  
Group Ii ◽  
Experimental Group

Abstract Objective: To examine the effects of gradually increasing force generated by permanent rare earth magnets for orthodontic tooth movement by using a novel experimental rat model and computer simulation. Materials and Methods: Fifty-five male rats (age 18 weeks) were used as animal experiments. Magnetic (experimental groups) or titanium (control group) cuboids (1.5 × 1.5 × 0.7 mm) were bonded to the lingual surface of the maxillary first molars. The initial distance between materials was 1.0 mm, generating 4.96 gf (experimental group I), or 1.5 mm, generating 2.26 gf (experimental group II). Tooth movement was measured and periodontal structures were observed with microfocus x-ray computed tomography radiographs. Results: The distance between the magnets decreased with time in experimental groups I and II (P &lt; .001), whereas there was no tooth displacement in the control group. Experimental group I showed rapid tooth movement in the initial phase followed by slower tooth movement. Experimental group II showed gradual tooth movement. Horizontal sections on microfocus computed tomgraphy radiographs revealed no pathological changes, such as root resorption, on the compressed side in the experimental groups. Conclusions: The initial light force and gradual increasing force in magnetic attractive force induced effective tooth movement in rats without inducing any pathological changes.

Sign in / Sign up

Export Citation Format

Share Document