scholarly journals Inhibitory effect of gels loaded with a low concentration of antibiotics against biofilm formation by Enterococcus faecalis and Porphyromonas gingivalis

2015 ◽  
Vol 57 (3) ◽  
pp. 213-218 ◽  
Author(s):  
Amnah A. Algarni ◽  
Ghaeth H. Yassen ◽  
Richard L. Gregory
Keyword(s):  
Enterococcus Faecalis ◽  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Low Concentration

Inhibitory effect of cranberry polyphenol on biofilm formation and cysteine proteases of Porphyromonas gingivalis

2007 ◽  
Vol 42 (6) ◽  
pp. 589-592 ◽  
Author(s):  
A. Yamanaka ◽  
T. Kouchi ◽  
K. Kasai ◽  
T. Kato ◽  
K. Ishihara ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Cysteine Proteases ◽  
Inhibitory Effect

scholarly journals Enterococcus faeciumWB2000 Inhibits Biofilm Formation by Oral Cariogenic Streptococci

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Nao Suzuki ◽  
Masahiro Yoneda ◽  
Yuko Hatano ◽  
Tomoyuki Iwamoto ◽  
Yosuke Masuo ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Mutans Streptococci ◽  
Streptococcus Sobrinus ◽  
Dual Cultures

This study investigated the inhibitory effect of probioticEnterococcus faeciumWB2000 on biofilm formation by cariogenic streptococci. The ability ofE. faeciumWB2000 and JCM5804 andEnterococcus faecalisJCM5803 to inhibit biofilm formation by seven laboratory oral streptococcal strains and 13 clinical mutans streptococcal strains was assayed. TheEnterococcalstrains inhibited biofilm formation in dual cultures with the mutans streptococcal strainsStreptococcus mutansXc andStreptococcus sobrinusJCM5176 (P<0.05), but not with the noncariogenic streptococcal strains.Enterococcus faeciumWB2000 inhibited biofilm formation by 90.0% (9/10) of the clinicalS. mutansstrains and 100% (3/3) of the clinicalS. sobrinusstrains. After culturing, the pH did not differ between single and dual cultures. The viable counts of floating mutans streptococci were lower in dual cultures withE. faeciumWB2000 than in single cultures.Enterococcus faeciumWB2000 acted as a probiotic bacterial inhibitor of cariogenic streptococcal biofilm formation.

scholarly journals Strawberry Extract’s Effects on Enterococcus faecalis and Porphyromonas gingivalis Biofilms in vitro

2017 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Armelia Sari Widyarman ◽  
Stephanie Brigitta Widjaja ◽  
Erik Idrus
Keyword(s):  
Enterococcus Faecalis ◽  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Oral Bacteria ◽  
Inhibition Effect ◽  
Extract Concentration ◽  
Significant Difference ◽  
Negative Controls ◽  
Optimal Incubation

<p><strong>Background</strong>: <em>Enterococcus faecalis </em>(<em>E. faecalis</em>)<em> </em>and<em> Porphyromonas gingivalis</em> (<em>P. gingivalis</em>) are oral bacteria related to root canal infection and periodontal disease pathogenesis. Strawberries (<em>Fragaria x ananassa</em>) fruit are rich in vitamins and minerals, have antibacterial and antioxidant effects.<strong> Objective</strong>: This study investigated the inhibition effect of strawberry extract on monospecies and multispecies <em>E. faecalis </em>and<em> P. gingivalis </em>bacteria grown as biofilms<em> in vitro.</em> <strong>Methods:</strong> This study used <em>E. faecalis</em> ATCC 29212 <em>and P. gingivalis</em> ATCC 33277. It analyzed<strong> </strong>the effect of strawberry extract on bacteria biofilm formation using a biofilm assay on microplate wells. Five concentrations of strawberry extracts were used (100%, 50%, 25%, 12.5%, and 6.25%), and the inhibition effect was observed after a 1h, 3h, 6h, and 24h incubation period. Biofilms without the strawberry extract were used as the negative controls, and crystal violet and safranin (0.5%<sup>w</sup>/<sub>v</sub>) were used to count the biofilm mass. The biofilms grown on microplates were counted using an ELISA reader at 450 nm after 200 mL of 90% ethanol was added to attract the absorbed stain. The strawberry extract inhibition effectiveness on the biofilm formation of each bacterium tested was analyzed using one-way Anova, where p&lt;0.05 was defined as a significant difference. <strong>Result</strong>: The strawberry extract inhibited the tested monospecies and multispecies bacteria biofilm formation. The optimal strawberry extract concentration for the inhibition of either monospecies biofilms was 100%. However, the optimal incubation time for the strawberry extract to inhibit the multispecies biofilm formation was 24h, which was the study’s biofilm maturity phase.<strong> Conclusions: </strong>The 100%<strong> </strong>strawberry extract concentration inhibited the formation of both the monospecies and multispecies <em>E. faecalis </em>and <em>P. gingivalis</em> biofilms. Future studies are needed to evaluate the potential of strawberry extract as an alternative dental therapy.</p>

scholarly journals Myricetin as an Antivirulence Compound Interfering with a Morphological Transformation into Coccoid Forms and Potentiating Activity of Antibiotics against Helicobacter pylori

2021 ◽  
Vol 22 (5) ◽  
pp. 2695
Author(s):  
Paweł Krzyżek ◽  
Paweł Migdał ◽  
Emil Paluch ◽  
Magdalena Karwańska ◽  
Alina Wieliczko ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Morphological Transformation ◽  
High Tendency ◽  
Minimal Inhibitory Concentrations ◽  
Coccoid Form ◽  
Stomach Diseases ◽  
Fold Reduction ◽  
H Pylori

Helicobacter pylori, a gastric pathogen associated with a broad range of stomach diseases, has a high tendency to become resistant to antibiotics. One of the most important factors related to therapeutic failures is its ability to change from a spiral to a coccoid form. Therefore, the main aim of our original article was to determine the influence of myricetin, a natural compound with an antivirulence action, on the morphological transformation of H. pylori and check the potential of myricetin to increase the activity of antibiotics against this pathogen. We observed that sub-minimal inhibitory concentrations (sub-MICs) of this compound have the ability to slow down the process of transformation into coccoid forms and reduce biofilm formation of this bacterium. Using checkerboard assays, we noticed that the exposure of H. pylori to sub-MICs of myricetin enabled a 4–16-fold reduction in MICs of all classically used antibiotics (amoxicillin, clarithromycin, tetracycline, metronidazole, and levofloxacin). Additionally, RT-qPCR studies of genes related to the H. pylori morphogenesis showed a decrease in their expression during exposure to myricetin. This inhibitory effect was more strongly seen for genes involved in the muropeptide monomers shortening (csd3, csd6, csd4, and amiA), suggesting their significant participation in the spiral-to-coccoid transition. To our knowledge, this is the first research showing the ability of any compound to synergistically interact with all five antibiotics against H. pylori and the first one showing the capacity of a natural substance to interfere with the morphological transition of H. pylori from spiral to coccoid forms.

Evaluation of antibacterial, teratogenicity and antibiofilm effect of sulfated chitosans extracted from marine waste against microorganism

2021 ◽  
pp. 088391152110142
Author(s):  
Velu Gomathy ◽  
Venkatesan Manigandan ◽  
Narasimman Vignesh ◽  
Aavula Thabitha ◽  
Ramachandran Saravanan
Keyword(s):  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Marine Litter ◽  
Ionization Time ◽  
Gram Positive Bacteria ◽  
Mineral Components ◽  
Ft Ir

Biofilms play a key role in infectious diseases, as they may form on the surface and persist after treatment with various antimicrobial agents. The Staphylococcus aureus, Klebsiella pneumoniae, S. typhimurium, P. aeruginosa, and Escherichia coli most frequently associated with medical devices. Chitosan sulphate from marine litter (SCH-MW) was extracted and the mineral components were determined using atomic absorption spectroscopy (AAS). The degree of deacetylation (DA) of SCH was predicted 50% and 33.3% in crab and shrimp waste respectively. The elucidation of the structure of the SCH-MW was portrayed using FT-IR and 1H-NMR spectroscopy. The molecular mass of SCH-MW was determined with Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF). The teratogenicity of SCH-MW was characterized by the zebrafish embryo (ZFE) model. Antimicrobial activity of SCH-MW was tested with the agar well diffusion method; the inhibitory effect of SCH-MW on biofilm formation was assessed in 96 flat well polystyrene plates. The result revealed that a low concentration of crab-sulfated chitosan inhibited bacterial growth and significantly reduced the anti-biofilm activity of gram-negative and gram-positive bacteria relatively to shrimp. It is potentially against the biofilm formation of pathogenic bacteria.

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