Applying CRISPR screen in Diabetes Research

Diabetes ◽  
2021 ◽  
pp. dbi200047
Author(s):  
Peng Yi ◽  
Noelle Morrow
Keyword(s):  
Diabetes Research ◽  
Crispr Screen

scholarly journals Applying CRISPR Screen in Diabetes Research

2021 ◽  
Author(s):  
Peng Yi ◽  
Noelle Morrow
Keyword(s):  
Cell Biology ◽  
Cancer Biology ◽  
Cell Types ◽  
Diabetes Research ◽  
Genetic Screens ◽  
Multiple Organs ◽  
Genome Wide ◽  
Forward Genetic Screens ◽  
Crispr Screen ◽  
Single Cell Type

The CRISPR/Cas9 genome editing system has been one of the greatest scientific discoveries in the last decade. The highly efficient and precise editing ability of this technology is of great therapeutic value and benefits the basic sciences as an advantageous research tool. In recent years, forward genetic screens utilizing CRISPR technology have been widely adopted, with genome-wide or pathway-focused screens leading to important and novel discoveries. CRISPR screens have been used primarily in cancer biology, virology and basic cell biology; but they have rarely been applied to diabetes research. A potential reason for this is that diabetes related research can be more complicated, often involving cross-talk between multiple organs or cell types. Nevertheless, many questions can still be reduced to the study of a single cell type if assays are carefully designed. Here we review the application of CRISPR screen technology and provide perspective on how it can be used in diabetes research.

scholarly journals Applying CRISPR Screen in Diabetes Research

2021 ◽  
Author(s):  
Peng Yi ◽  
Noelle Morrow
Keyword(s):  
Cell Biology ◽  
Cancer Biology ◽  
Cell Types ◽  
Diabetes Research ◽  
Genetic Screens ◽  
Multiple Organs ◽  
Genome Wide ◽  
Forward Genetic Screens ◽  
Crispr Screen ◽  
Single Cell Type

The CRISPR/Cas9 genome editing system has been one of the greatest scientific discoveries in the last decade. The highly efficient and precise editing ability of this technology is of great therapeutic value and benefits the basic sciences as an advantageous research tool. In recent years, forward genetic screens utilizing CRISPR technology have been widely adopted, with genome-wide or pathway-focused screens leading to important and novel discoveries. CRISPR screens have been used primarily in cancer biology, virology and basic cell biology; but they have rarely been applied to diabetes research. A potential reason for this is that diabetes related research can be more complicated, often involving cross-talk between multiple organs or cell types. Nevertheless, many questions can still be reduced to the study of a single cell type if assays are carefully designed. Here we review the application of CRISPR screen technology and provide perspective on how it can be used in diabetes research.

209-OR: ADA Presidents' Select Abstract: Type 1 Diabetes and the Developing Brain—A Longitudinal Study of Brain Growth by the Diabetes Research in Children Network (DirecNet)

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 209-OR ◽  
Author(s):  
ANA MARIA ARBELAEZ ◽  
STEFANI O’DONOGHUE ◽  
NELLY MAURAS ◽  
BRUCE A. BUCKINGHAM ◽  
NEIL H. WHITE ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Longitudinal Study ◽  
Developing Brain ◽  
Diabetes Research ◽  
Brain Growth ◽  
Select Abstract

scholarly journals An overview of the rationale for qualitative research methods in social health

2013 ◽  
Vol 01 (01) ◽  
pp. 006-008
Author(s):  
Heather Stuckey
Keyword(s):  
Qualitative Research ◽  
Health Care Professionals ◽  
Quantitative Methods ◽  
Diabetes Management ◽  
Social Health ◽  
Diabetes Research ◽  
Medical Outcomes ◽  
Qualitative And Quantitative ◽  
Qualitative And Quantitative Methods ◽  
Beliefs And Attitudes

Qualitative research is a primary way to understand the context of diabetes in a person′s life, beyond the medical outcomes. Identifying the qualitative issues such as patients′ knowledge about diabetes, their beliefs and attitudes, and their relationship with health care professionals can serve as data to determine the obstacles and, in turn, resolutions to those issues in diabetes management. Characteristics of qualitative and quantitative methods are described, with the discussion that both methods are complementary, not conflicting, to further the field of diabetes research.

scholarly journals Bioengineered Islet Cell Transplantation

2021 ◽  
Author(s):  
Kevin Bellofatto ◽  
Beat Moeckli ◽  
Charles-Henri Wassmer ◽  
Margaux Laurent ◽  
Graziano Oldani ◽  
...  
Keyword(s):  
State Of The Art ◽  
Diabetes Research ◽  
Islet Cell Transplantation ◽  
Β Cell ◽  
Cell Compartment ◽  
Factors Affecting ◽  
Insulin Activity ◽  
On Chip

Abstract Purpose of Review β cell replacement via whole pancreas or islet transplantation has greatly evolved for the cure of type 1 diabetes. Both these strategies are however still affected by several limitations. Pancreas bioengineering holds the potential to overcome these hurdles aiming to repair and regenerate β cell compartment. In this review, we detail the state-of-the-art and recent progress in the bioengineering field applied to diabetes research. Recent Findings The primary target of pancreatic bioengineering is to manufacture a construct supporting insulin activity in vivo. Scaffold-base technique, 3D bioprinting, macro-devices, insulin-secreting organoids, and pancreas-on-chip represent the most promising technologies for pancreatic bioengineering. Summary There are several factors affecting the clinical application of these technologies, and studies reported so far are encouraging but need to be optimized. Nevertheless pancreas bioengineering is evolving very quickly and its combination with stem cell research developments can only accelerate this trend.

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