The Impact of the Demineralized Organic Matrix on the Effect of TiF4 Varnish on the Progression of Dentin Erosive Loss

2017 ◽  
Vol 51 (3) ◽  
pp. 264-270 ◽  
Author(s):  
Beatriz Martines de Souza ◽  
Mariele Vertuan ◽  
Marília Afonso Rabelo Buzalaf ◽  
Ana Carolina Magalhães
Keyword(s):  
Citric Acid ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Organic Matrix ◽  
Analysis Data ◽  
Acid Ph ◽  
Bonferroni Test ◽  
And Control ◽  
The Impact

This in vitro study compared the effect of TiF4 varnish with that of NaF varnish, applied on pre-eroded bovine dentin samples, with respect to the progression of erosive loss, in the presence or absence of the demineralized organic matrix (DOM). One hundred and sixty bovine dentin samples were pre-eroded (0.1% citric acid, pH 2.5, 30 min). Half of the samples were subjected to the DOM removal (collagenase solution, 5 days). Samples with and without the DOM were treated according to the groups (n = 20 with DOM and 20 without DOM/group): TiF4 varnish (2.45% F), NaF varnish (2.45% F), placebo varnish (without fluoride) and control (no treatment). Thereafter, the treated samples were submitted to erosive challenges 4 × 90 s/day (0.1% citric acid, pH 2.5) during 7 days. Between the challenges, the samples were immersed in artificial saliva. The dentin erosive loss was measured using contact profilometry (µm, n = 15). Five dentin samples per group were prepared for energy-dispersive X-ray spectroscopy analysis. Data were compared using 2-way ANOVA/Bonferroni test (p < 0.05). Both fluoride varnishes were effective in reducing the erosive loss progression regardless of the dentin condition when compared to placebo varnish and control groups. Despite the fact that the TiF4 varnish was more effective than the NaF varnish for both dentin conditions (p < 0.001), its effect was significantly reduced in the absence of DOM (p < 0.05). It can be concluded that the TiF4 varnish is the best treatment in reducing the progression of dentin erosive loss (100%) in vitro, but its protective effect is more pronounced in the presence of DOM.

scholarly journals Fluoride Release from Fluoride Varnishes under Acidic Conditions

2014 ◽  
Vol 39 (1) ◽  
pp. 35-39 ◽  
Author(s):  
F Lippert
Keyword(s):  
Citric Acid ◽  
Artificial Saliva ◽  
Fluoride Varnish ◽  
Laboratory Research ◽  
Fluoride Release ◽  
Acidic Solutions ◽  
Acid Ph ◽  
Opposite Behavior ◽  
Acidic Conditions

Objective: The aim was to investigate the in vitro fluoride release from fluoride varnishes under acidic conditions. Study design: Poly(methyl methacrylate) blocks (Perspex, n=3 per group) were painted with 80±5 mg fluoride varnish (n=10) and placed into artificial saliva for 30min. Then, blocks were placed into either 1% citric acid (pH 2.27) or 0.3% citric acid (pH 3.75) solutions (n=3 per solution and varnish) for 30min with the solutions being replaced every 5min. Saliva and acid solutions were analyzed for fluoride content. Data were analyzed using three-way ANOVA (varnish, solution, time). Results: The three-way interaction was significant (p&gt;0.0001). Fluoride release and release patterns varied considerably between varnishes. Fluoride release in saliva varied by a factor of more than 10 between varnishes. Some varnishes (CavityShield, Nupro, ProFluorid, Vanish) showed higher fluoride release in saliva than during the first 5min of acid exposure, whereas other varnishes (Acclean, Enamel-Pro, MI Varnish, Vella) showed the opposite behavior. There was little difference between acidic solutions. Conclusions: Fluoride release from fluoride varnishes varies considerably and also depends on the dissolution medium. Bearing in mind the limitations of laboratory research, the consumption of acidic drinks after fluoride varnish application should be avoided to optimize the benefit/risk ratio.

scholarly journals The Effects of Three Chlorhexidine-Based Mouthwashes on Human Osteoblast-Like SaOS-2 Cells. An In Vitro Study

2021 ◽  
Vol 22 (18) ◽  
pp. 9986
Author(s):  
Giulia Brunello ◽  
Kathrin Becker ◽  
Luisa Scotti ◽  
Dieter Drescher ◽  
Jürgen Becker ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cetylpyridinium Chloride ◽  
Control Cell ◽  
In Vitro Study ◽  
Control Group ◽  
Sterile Saline ◽  
Lack Of Information ◽  
And Control ◽  
The Impact

Several decontamination methods for removing biofilm from implant surfaces during surgical peri-implantitis treatment have been reported, including the intraoperative usage of chlorhexidine (CHX)-based antiseptics. There is a lack of information on possible adverse effects on bone healing. The study aimed to examine the impact of three CHX-based mouthwashes on osteoblast-like cells (SaOS-2) in vitro. Cells were cultured for three days in 96-well binding plates. Each well was randomly treated for either 30, 60 or 120 s with 0.05% CHX combined with 0.05% cetylpyridinium chloride (CPC), 0.1% CHX, 0.2% CHX or sterile saline (NaCl) as control. Cell viability, cytotoxicity and apoptosis were assessed at day 0, 3 and 6. Cell viability resulted in being higher in the control group at all time points. At day 0, the CHX 0.2 group showed significantly higher cytotoxicity values compared to CHX 0.1 (30 s), CHX + CPC (30 s, 60 s and 120 s) and control (60 s and 120 s), while no significant differences were identified between CHX + CPC and both CHX 0.1 and NaCl groups. All test mouthwashes were found to induce apoptosis to a lower extent compared to control. Results indicate that 0.2% CHX presented the highest cytotoxic effect. Therefore, its intraoperative use should be carefully considered.

scholarly journals IMPACT OF LIGHT AND SUGAR-FREE BEVERAGES ON DENTIN EROSION - AN IN VITRO STUDY

2014 ◽  
Vol 2 (2) ◽  
pp. 159
Author(s):  
Ana Luiza Ubinha Moino ◽  
Catarina Ribeiro Barros Alencar ◽  
Maisa Camilo Jordão ◽  
Marília Afonso Rabelo Buzalaf ◽  
Ana Carolina Magalhães ◽  
...  
Keyword(s):  
Fruit Juice ◽  
Artificial Saliva ◽  
Erosive Wear ◽  
In Vitro Study ◽  
Tooth Wear ◽  
Passion Fruit ◽  
Root Dentin ◽  
Bonferroni Test ◽  
Dentin Erosion

AIM: To compare the erosive effect of different light and sugar free beverages in relation to their regular version, on dentin. MATERIALS AND METHODS: Bovine root dentin samples were randomized into 9 different types of drinks (each n=12): Coca-cola®, Light Coca-cola®, Coca-cola® Zero, Guaraná Antartica®, Guaraná Antartica® Zero, Sprite®, Sprite® Zero, Del Valle® Passion fruit juice and Del Valle® Light Passion fruit juice. During each one of the 10 experimental days, the samples were subjected to 3 erosive demineralization (by immersion of the sample in the studied drink) and remineralization (by immersion of the sample in artificial saliva) cycles. Profilometry was evaluated at the end of the 1st, 5th and 10th days. For each type of drink, the data were tested using 2 way ANOVA and Bonferroni test (p<0.05). RESULTS: Erosive cycling with regular cola resulted in a higher wear on the first day, on the 5th day the regular drink was similar to zero and greater than light version, on the 10th day there was no difference between the versions of cola drink. For the Sprite drink there was difference between the versions just in the 10th day, in which the higher wear was promoted by the zero version. For guaraná and juice beverages there was no differences between versions in relation to the resulted erosive wear. CONCLUSION: Erosive tooth wear promoted by light and sugar-free or regular versions of soft drinks and juices showed distinct behaviours over the cycling time.

scholarly journals Mechanical Properties of Thermoplastic Polymers for Aligner Manufacturing: In Vitro Study

2020 ◽  
Vol 8 (2) ◽  
pp. 47 ◽  
Author(s):  
Francesco Tamburrino ◽  
Vincenzo D’Antò ◽  
Rosaria Bucci ◽  
Giulio Alessandri-Bonetti ◽  
Sandro Barone ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Elastic Modulus ◽  
Yield Stress ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Tensile Tests ◽  
Operating Conditions ◽  
Thermoplastic Polymers ◽  
The Impact

The use of metal-free thermoplastic materials plays a key role in the orthodontic digital workflow due to the increasing demand for clear aligner treatments. Three thermoplastic polymers commonly used to fabricate clear aligners, namely Duran®, Biolon® and Zendura®, were investigated to evaluate the effect of thermoforming (T.), storage in artificial saliva (S.A.S.) and their combination on their mechanical properties. Elastic modulus and yield stress of the specimens were characterized. Each material was characterized for each condition through tensile tests (ISO527-1). The results showed that thermoforming does not lead to a significant decrease in yield stress, except for Zendura® that showed about a 30% decrease. An increase of the elastic modulus of Duran® and Zendura®, instead, was observed after thermoforming. The same increase was noticed for the yield stress of Duran®. For S.A.S. specimens, the elastic modulus generally decreases compared to supplier condition (A.S.) and simply thermoformed material. A decrease of yield stress, instead, is significant for Zendura®. The results demonstrated that the impact of the operating conditions on the mechanical properties can vary according to the specific polymer. To design reliable and effective orthodontic treatments, the materials should be selected after their mechanical properties are characterized in the simulated intraoral environment.

In vitro Study of the Role of Human Neutrophil Enzymes on Root Caries Progression

2021 ◽  
pp. 1-9
Author(s):  
Camila A. Zamperini ◽  
Berdan Aydin ◽  
Herve Y. Sroussi ◽  
Ana Karina Bedran-Russo
Keyword(s):  
Human Neutrophil ◽  
In Vitro Study ◽  
Organic Matrix ◽  
Root Surface ◽  
Host Immune System ◽  
Root Caries ◽  
Caries Progression ◽  
And Control

The role of the host immune system in caries progression is mainly speculative, and it is believed that it entails the enzymatic degradation of the dentin organic matrix. The aim of this study was to evaluate the proteolytic effect of human neutrophil enzymes on root caries progression. For this, specimens of bovine root dentin were divided into 4 groups (<i>n</i> = 30): caries (C), caries + neutrophils (C + N), no caries (Control), and no caries + neutrophils (Control + N). <i>Streptococcus mutans</i> biofilm (10<sup>5</sup> CFU/mL) was grown on the root surface to artificially induce root carious lesions (C and C + N groups). Specimens were then exposed to neutrophils (5 × 10<sup>6</sup> cells/mL) for 48 h (C + N and Control + N groups). Caries development and neutrophil exposures were repeated a 2nd and 3rd time. Caries depth (CD) and dentin demineralization (DD) were assessed by infiltration of rhodamine B using fluorescence microscopy. Collagen fibril ultrastructure was characterized under a polarized microscope with Picrosirius red staining. There were no significant differences (<i>p</i> &#x3e; 0.05) in CD and DD between the C and C + N groups for 1, 2, and 3 caries-neutrophil exposures. Immature collagen was significantly less present in the carious groups (C, <i>p</i> = 0.003; C + N, <i>p</i> = 0.01) than in the noncarious groups in the most superficial 200 µm. We thus concluded that human neutrophil enzymes did not influence short-term root caries progression, and immature collagen fibrils were more susceptible to degradation during <i>S. mutans</i>-induced root caries progression.

scholarly journals Effects on Dentin Treated with Eluted Multi-Mineral Varnish In Vitro

2012 ◽  
Vol 6 (1) ◽  
pp. 157-163 ◽  
Author(s):  
Robert L Karlinsey ◽  
Allen C Mackey ◽  
Craig S Schwandt
Keyword(s):  
Citric Acid ◽  
Artificial Saliva ◽  
Control Group ◽  
Distilled Water ◽  
Water Control ◽  
Acid Ph ◽  
Ph Cycling ◽  
Demineralized Dentin ◽  
Acid Challenge

Purpose: The purpose of this pilot study was to assess the non-contact effects of a NaF varnish on the morphology and elemental compositions of sound and demineralized dentin in an in vitro pH cycling model. Methods: 3 mm diameter dentin cores were extracted from bovine teeth, mounted in acrylic rods, and ground and polished. Sound and demineralized (in 10 mL unstirred 50% citric acid (pH = 1.2) for two minutes) specimens were divided into four groups (N=6) corresponding to treatments with either water (control group) or non-contact Vanish® 5% NaF White Varnish with tri-calcium phosphate (3M ESPE). For the non-contact varnish group, one 0.50 ml unit-dose was applied to acrylic rods and positioned in the same stopper as three dentin specimens. The four groups were then cycled in a regimen consisting of three rounds of one-hour treatments with each round followed by immersion in a three-minute static acid challenge (0.3% citric acid, pH = 3.8). For each one-hour treatment immersion the test groups were immersed in distilled water. The specimens were thoroughly rinsed with distilled water after each treatment and after each acid challenge. After the last challenge, specimens were rinsed with distilled water and immersed in artificial saliva (AS) overnight. All treatments, acid challenges and immersion in AS solution were performed in an incubator set at 37°C. After three days, the specimens were assessed using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). Results: SEM and EDS results demonstrated the non-contact varnish group can provide mineralization benefits relative to the control group for both sound and demineralized dentin substrates. Depositions from mineral eluting from the varnish developed on the intertubular and intratubular regions alike, with the most prominent depositions appearing on initially demineralized dentin. Conclusions: In this observational study, acid-resistant mineral depositions were produced on the sound and dentin surfaces without direct application from a multi-mineral varnish containing fluoride, calcium and phosphate.

scholarly journals Effects of Two In-Office Bleaching Agents with Different pH on the Structure of Human Enamel: An In Situ and In Vitro Study

2013 ◽  
Vol 38 (1) ◽  
pp. 100-110 ◽  
Author(s):  
Y Sa ◽  
L Sun ◽  
Z Wang ◽  
X Ma ◽  
S Liang ◽  
...  
Keyword(s):  
Artificial Saliva ◽  
In Vitro Study ◽  
Storage Condition ◽  
Human Saliva ◽  
Low Ph ◽  
Force Microscopy ◽  
Human Enamel ◽  
And Control

SUMMARY This study evaluated the effects of two in-office bleaching agents (Beyond and Opalescence Boost) with different pH on the structure and mechanical properties of human enamel in vitro and in situ. One hundred and eight enamel slabs were obtained from freshly extracted premolars. The specimens were randomly distributed into nine groups (n=12), and the human saliva (HS) in the volunteers' oral cavities was used to simulate the in situ condition: Beyond + HS, Opalescence Boost (O-Boost) + HS, Control + HS, Beyond + artificial saliva (AS), O-Boost + AS, Control + AS, Beyond + distilled water (DW), O-Boost + DW, and Control + DW. The bleaching treatments were performed on the first and eighth day, and the total bleaching time was 90 minutes. Baseline and final surface roughness (RMS), surface morphology, microhardness, and fracture toughness (FT) were measured before the treatment and on the fifteenth day, respectively. Compared with control groups, surface alterations on enamel were found in the Beyond + AS and Beyond + DW groups under atomic force microscopy evaluation. Two-way analysis of variance and Tukey test revealed that the RMS showed significant intergroup differences for both storage condition and bleaching agent, whereas microhardness and FT revealed no significant alteration. The results indicated that in-office bleaching agents with low pH values could induce enamel morphology alterations under in vitro conditions. The presence of natural HS could eliminate the demineralization effect caused by low pH.

scholarly journals Effect of CPP-ACP on enamel eroded extrinsically: in vitro study

2018 ◽  
Vol 66 (1) ◽  
pp. 21-28
Author(s):  
Iandara de Lima SCARDINI ◽  
Marcella Kretli Neves DIAS ◽  
Martha Chiabai Cupertino de CASTRO ◽  
Ana Paula Albuquerque GUEDES ◽  
Letícia Monteiro PEIXOTO
Keyword(s):  
Citric Acid ◽  
Tooth Enamel ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Deionized Water ◽  
Statistical Testing ◽  
Immersion Medium ◽  
Significant Difference ◽  
Before And After

ABSTRACT Objective: The aim of this study was to evaluate the remineralization effect of CPP-ACP on tooth enamel that is eroded extrinsically. Methods: Sixty freshly extracted bovine teeth were stored in a solution of thymol (0.1%), cut into enamel blocks (6x6mm), abraded with silicon carbide sandpaper (600, 800 and 1200 grit) and polished with felt. The specimens were randomly divided into 4 groups (n=15), varying the immersion medium , deionized water or citric acid, and the optional use of CPP-ACP. (G1: deionized water; G2: citric acid; G3: deionized water + CPP-ACP; G4: citric acid + CPP-ACP). The samples were subjected to 4 cycles of erosion every day, comprising 5 minutes immersion in one medium and 2 hours of immersion in artificial saliva, for 5 consecutive days. After each erosive cycle, 20 uL of the MI Paste was applied for 5 minutes on one half of the samples and washed with deionized water. Before and after the erosive cycling, the measurements of Vickers hardness and surface roughness were taken. Results: The results were evaluated through statistical testing. The final hardness and roughness values showed no statistically significant difference between G2 and G4. The paste in G4 did not promote an increase in hardness values nor a decrease in roughness values. Conclusion: It was concluded that CPP-ACP was unable to remineralize enamel subjected to extrinsic erosion, being ineffective in preventing erosion of enamel and in the remineralization of the eroded enamel.

scholarly journals Impact of Various Irrigating Agents on Root Fracture: An in vitro Study

2016 ◽  
Vol 17 (8) ◽  
pp. 659-662 ◽  
Author(s):  
Pradnya Nikhade ◽  
Sukriti Tiwari ◽  
C Sudarshan ◽  
Priyadarshini Shetty ◽  
Naveen K Gupta
Keyword(s):  
Citric Acid ◽  
Fracture Resistance ◽  
Ethylenediaminetetraacetic Acid ◽  
In Vitro Study ◽  
Study Groups ◽  
Test Machine ◽  
Root Fracture ◽  
Level Of Significance ◽  
The Impact

ABSTRACT Introduction Irrigating solutions are used for cleaning and removing dentinal debris, and the other remains from pulpal space during biomechanical preparation. Therefore, we evaluated the impact of various irrigating agents on root fracture at 5-minute time exposure. Materials and methods We sectioned 60 permanent maxillary premolars with fully formed root structures transversely maintaining the root length of approximately 14 mm. Five study groups were made comprising ethylenediaminetetraacetic acid (EDTA), cetrimide, citric acid, and so on as various irrigating agents. A universal force test machine was used to calculate the force which was enough to fracture each root. Analysis of variance (ANOVA) test was used to access the level of significance. Results About 10% citric acid solution as an irrigating agent showed minimal fracture opposing results, whereas 10% EDTA solution showed the maximum fracture resistance of root portion. Clinical significance Selection of suitable EDTA concentration that has minimal adverse effect on the mechanical properties of the tooth is very important for the successful management of tooth fracture. Conclusion About 10% EDTA provided the highest fracture resistance, necessitating the use of irrigating solution in root canal therapy (RCT). Further research with higher and different study groups is required to search for more efficient irrigating solution to improve the outcome of RCT. How to cite this article Tiwari S, Nikhade P, Chandak M, Sudarshan C, Shetty P, Gupta NK. Impact of Various Irrigating Agents on Root Fracture: An in vitro Study. J Contemp Dent Pract 2016;17(8):659-662

scholarly journals In Vitro Effects of Resin Infiltration on Enamel Erosion Inhibition

2015 ◽  
Vol 40 (5) ◽  
pp. 492-502 ◽  
Author(s):  
GC Oliveira ◽  
AP Boteon ◽  
FQ Ionta ◽  
MJ Moretto ◽  
HM Honório ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Adhesive System ◽  
Dental Erosion ◽  
Deep Penetration ◽  
Resin Infiltration ◽  
Fluorescence Confocal Microscopy ◽  
In Vitro Effects

SUMMARY Resin-based materials that show promising effects for preventing the progression of erosion have been studied. This in vitro study evaluated the effects of applying resin-based materials, including resin infiltration, on previously eroded enamel subjected to erosive challenges. The influence of enamel surface etching prior to application of the material was also studied. Bovine enamel blocks were immersed in hydrochloric acid (HCl), 0.01 M (pH 2.3), for 30 seconds in order to form a softened erosion lesion. The blocks were then randomly divided into nine groups (n=12) and treated as follows: C = control without treatment; Hel = pit & fissure resin sealant (Helioseal Clear); Adh = two-step self-etching adhesive system (AdheSe); Tet = two-step conventional adhesive system (Tetric N-bond); and Inf = infiltrant (Icon). The Helno, Adhno, Tetno, and Infno groups received the same materials without (or with no) surface conditioning. The depth of the material's penetration into softened erosion lesions was qualitatively analyzed using reflection and fluorescence confocal microscopy. After application of the materials, the blocks were immersed in HCl for two minutes; this step was followed by immersion in artificial saliva for 120 minutes four times a day for five days (erosive cycling). Both the enamel alteration and material thickness were analyzed using profilometry, and the results were submitted to Kruskal-Wallis and Dunn tests (p&gt;0.05). Images from the confocal microscopy showed minimal penetration of Adh/Adhno and deep penetration of Inf/Infno into the erosive lesions. The groups Hel, Adh, Inf, Tetno, and Infno resulted in the formation of a layer of material over the enamel, which was effective in inhibiting the progression of erosion. In conclusion, the infiltrant, with or without etching, was able to penetrate and protect the enamel against dental erosion. The other resin-based materials, except for the two-step conventional adhesive, were able to penetrate and inhibit the progression of erosive lesions only when they were applied after enamel etching.

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