Clinical and Histological Study on Direct Pulp Capping With CO2 Laser Irradiation in Human Teeth

SUMMARYThe study aimed to histologically evaluate wound healing of exposed human pulp on direct pulp capping using super-pulsed CO2 laser preirradiation. In this single-blind clinical trial, 28 third molar teeth of 17 volunteers were randomly capped with either CO2 laser irradiation (n=14) or Dycal (calcium hydroxide cement; n=14) and restored using resin composite. The laser was operated in super-pulsed mode (pulse duration, 0.2 ms; interval, 5.8 ms; 0.003 J/pulse). The irradiation conditions were a power output of 0.5 W, an irradiation time of 15 seconds, repeat mode (10-ms irradiation and 10-ms intervals, for a total beam exposure time of 7.5 seconds), total applied energy of 3.75 J, and an activated air-cooling system. Each tooth was extracted at six or 12 months posttreatment and prepared for histological evaluation. We evaluated the parameters of pulp tissue disorganization, inflammatory cell infiltration, reparative dentin formation (RDF), and bacterial penetration. There were no significant differences between groups for all parameters at each postoperative period (Mann-Whitney U-test, p>0.05). CO2 laser irradiation completely controlled bleeding and exudate from the exposed pulp. The CO2 laser group had a tendency to delay RDF compared with the Dycal group, but 4 of 7 teeth from the CO2 laser group showed a complete dentin bridge at 12 months posttreatment.

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Melatonin as an Agent for Direct Pulp-Capping Treatment

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17031043 ◽

2020 ◽

Vol 17 (3) ◽

pp. 1043 ◽

Cited By ~ 3

Author(s):

Julia Guerrero-Gironés ◽

Antonia Alcaina-Lorente ◽

Clara Ortiz-Ruiz ◽

Eduardo Ortiz-Ruiz ◽

María P. Pecci-Lloret ◽

...

Keyword(s):

Oxidative Stress ◽

Dental Pulp ◽

Bone Growth ◽

Study Groups ◽

Histological Evaluation ◽

Sprague Dawley ◽

Direct Pulp Capping ◽

Pulp Capping ◽

Reparative Dentin ◽

Pulp Inflammation

Melatonin plays an essential role in the regulation of bone growth. The actions that melatonin exerts on odontoblasts may be similar to its action on osteoblasts. This research aimed to evaluate the pulp response to melatonin used for direct pulp capping to evaluate the antioxidant effect of melatonin administered orally and its influence on dental pulp. Direct pulp capping was performed on the upper molars of Sprague Dawley rats using melatonin or Mineral Trioxide Aggregate (MTA). The study groups were: MTA; Melatonin; MTA + Melatonin administered orally; and Melatonin + Melatonin administered orally. In the latter two groups, the animals drank water dosed with melatonin ad libitum (10 mg/100 mL). After 30 days, the animals were sacrificed, and 5 ml of blood, the kidneys, and the liver were extracted in order to evaluate oxidative stress using thiobarbituric acid reactive substances testing (TBARS). Fragments of the maxilla containing the study molars were prepared for histological evaluation. The degree of pulp inflammation and pulp necrosis, the presence of reparative dentin and dentin bridging the pulp chamber, the presence and regularity of the odontoblastic layer, and the presence of pulp fibrosis were evaluated. No significant differences were found between the four study groups for any of the studied histological variables. The oral administration of melatonin did not modify the local effects of MTA or melatonin on dental pulp, or reduce basal-level oxidative stress. The effect of melatonin on pulp is similar to that of MTA and may be used as an agent for direct pulp capping.

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Histological evaluation of human pulp capped with light-cured calcium based cements: a randomized controlled clinical trial

International Journal of Scientific Reports ◽

10.18203/issn.2454-2156.intjscirep20171926 ◽

2017 ◽

Vol 3 (5) ◽

pp. 120 ◽

Cited By ~ 3

Author(s):

G. Jeya Gopika ◽

Sathyanarayanan Ramarao ◽

Carounanidy Usha ◽

Bindu Meera John ◽

N. Vezhavendhan

Keyword(s):

Inflammatory Response ◽

Calcium Hydroxide ◽

Calcium Silicate ◽

Permanent Teeth ◽

Histological Evaluation ◽

Controlled Clinical Trial ◽

Direct Pulp Capping ◽

Hard Tissue ◽

Pulp Capping ◽

Reparative Dentin

Background: Calcium hydroxide has traditionally been used as the pulp capping material for pulpal exposures in permanent teeth. The tunnel defects in the barrier and the tendency for dissolution, however, fails to provide permanent protection to the pulp. Light curable resin based cements have been introduced to enable a better marginal seal and lesser dissolution. The purpose of this study was to compare and evaluate the response of human pulp following direct pulp capping with the new resin based Calcium silicate (TheraCal LC) and Calcium hydroxide with hydroxyapatite (Septocal LC) cements compared with calcium hydroxide (Dycal). Methods: 72 intact human premolars scheduled for orthodontic extractions were exposed to direct pulp capping procedures using three different pulp capping agents. Teeth were randomly divided into 3 groups, Group A: Dycal, Group B: TheraCal LC, Group C: Septocal LC. The teeth were extracted at the end of 15 and 40 days’ and were evaluated histologically. They were scored for reparative dentin formation and inflammatory response. Inferential statistics was done using Chi square test. Results: Majority of the specimens in all three groups at the end of 15 days’ showed partial to lateral deposition of hard tissue. There was continuous deposition of hard tissue and severe inflammatory response at the end of 40 days’ in Dycal. There was partial deposition of hard tissue and reduced inflammatory response at the end of 40 days’ in TheraCal LC and Septocal LC. However, the results were not statistically significant between the three groups at two different time periods. Conclusions: Light cured, Calcium silicate (TheraCal LC) and Calcium hydroxide with hydroxyapatite (Septocal LC) cements were as effective as calcium hydroxide (Dycal) in inducing the formation of reparative dentin and evoking inflammatory response.

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Histological evaluation of direct pulp capping of rat pulp with experimentally developed low-viscosity adhesives containing reparative dentin-promoting agents

Journal of Dentistry ◽

10.1016/j.jdent.2015.11.005 ◽

2016 ◽

Vol 44 ◽

pp. 27-36 ◽

Cited By ~ 7

Author(s):

Masaya Suzuki ◽

Yoshihisa Taira ◽

Chikage Kato ◽

Koichi Shinkai ◽

Yoshiroh Katoh

Keyword(s):

Histological Evaluation ◽

Direct Pulp Capping ◽

Pulp Capping ◽

Low Viscosity ◽

Reparative Dentin

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Clinical and Histological Evaluation of Direct Pulp Capping on Human Pulp Tissue Using a Dentin Adhesive System

BioMed Research International ◽

10.1155/2016/2591273 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 7

Author(s):

Alicja Nowicka ◽

Ryta Łagocka ◽

Mariusz Lipski ◽

Mirosław Parafiniuk ◽

Katarzyna Grocholewicz ◽

...

Keyword(s):

Calcium Hydroxide ◽

Third Molar ◽

Adhesive System ◽

Histological Evaluation ◽

Control Group ◽

Single Bond ◽

Pulp Tissue ◽

Pulp Capping ◽

Hydroxide Group ◽

Dentin Adhesive

Objective. This study presents a clinical and histological evaluation of human pulp tissue responses after direct capping using a new dentin adhesive system. Methods. Twenty-eight caries-free third molar teeth scheduled for extraction were evaluated. The pulps of 22 teeth were mechanically exposed and randomly assigned to 1 of 2 groups: Single Bond Universal or calcium hydroxide. Another group of 6 teeth acted as the intact control group. The periapical response was assayed, and a clinical examination was performed. The teeth were extracted after 6 weeks, and a histological analysis was performed. The pulp status was assessed, and the thickness of the dentin bridge was measured and categorized using a histological scoring system. Results. The clinical phase was asymptomatic for Single Bond Universal patients. Patients in the calcium hydroxide group reported mild symptoms of pain, although the histological examination revealed that dentin bridges with or without limited pulpitis had begun forming in each tooth. The universal adhesive system exhibited nonsignificantly increased histological signs of pulpitis (P>0.05) and a significantly weaker thin mineralized tissue layer (P<0.001) compared with the calcium hydroxide group. Conclusion. The results suggest that Single Bond Universal is inappropriate for human pulp capping; however, further long-term studies are needed to determine the biocompatibility of this agent.

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Histological Evaluation of Pulpal Response to Direct Pulp Capping with Biodentine and Mineral Trioxide Aggregate: An In Vivo Study

Journal of Operative Dentistry & Endodontics ◽

10.5005/jp-journals-10047-0080 ◽

2019 ◽

Vol 4 (2) ◽

pp. 57-61

Author(s):

Mahendran Kavitha ◽

Hema J Christa ◽

Nandhini Ambalavanan ◽

Shakthi Priya Sivaprakasam

Keyword(s):

Mineral Trioxide Aggregate ◽

In Vivo Study ◽

Histological Evaluation ◽

Direct Pulp Capping ◽

Pulp Capping

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Reparative Mineralized Tissue Characterization after Direct Pulp Capping with Calcium-Silicate-Based Cements

Materials ◽

10.3390/ma12132102 ◽

2019 ◽

Vol 12 (13) ◽

pp. 2102 ◽

Cited By ~ 4

Author(s):

Xuan Tran ◽

Hamideh Salehi ◽

Minh Truong ◽

Minic Sandra ◽

Jeremy Sadoine ◽

...

Keyword(s):

Chemical Composition ◽

Calcium Silicate ◽

Tissue Characterization ◽

Mineral Trioxide Aggregate ◽

Clinical Situation ◽

Mineralized Tissue ◽

Direct Pulp Capping ◽

Pulp Capping ◽

Inflammatory Status ◽

Reparative Dentin

Nowadays, the preservation of dental pulp vitality is an integral part of our daily therapies. The success of these treatments depends on the clinical situation as well as the biomaterials used. Mineral Trioxide aggregate and BiodentineTM are commonly used as pulp capping materials. One objective of vital pulp therapy is the repair/regeneration of the pulp. In addition to the initial inflammatory status of the pulp, the nature and quality of the new mineralized tissue obtained after pulp capping directly influence the success of the treatment. In order to characterize the reparative dentin, in the current study, the chemical composition and microstructure of the dentin bridge after direct pulp capping using Biodentine™ and mineral trioxide aggregate (MTA) was studied by using Raman microspectroscopy and scanning electron microscopy, respectively. The results showed that the reparative dentin bridge observed in both groups presented dentin tubules and chemical composition similar to primary dentin. With the limitations of this study, the calcium-silicate-based cements used as pulp capping materials provide an optimal environment for pulp healing, resulting in a reparative dentin resembling on certain points of the primary dentin and the regeneration of the pulp.

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Induction of reparative dentin by calcium silicate-based material as direct pulp capping agent

Bangabandhu Sheikh Mujib Medical University Journal ◽

10.3329/bsmmuj.v12i4.43857 ◽

2019 ◽

Vol 12 (4) ◽

pp. 182-186

Author(s):

Mozammal Hossain ◽

Mahmood Sajedeen ◽

Yukio Nakamura

Keyword(s):

Calcium Hydroxide ◽

Calcium Silicate ◽

Normal Function ◽

Direct Pulp Capping ◽

Pulp Tissue ◽

Pulp Capping ◽

Exposure Site ◽

Reparative Dentin ◽

Dentin Formation ◽

Reparative Dentin Formation

This study was performed to examine whether calcium silicate could induce reparative dentin formation without eliciting any adverse effect in direct pulp capping of premolar teeth. Twenty participants who need extraction of their 4 healthy permanent premolar teeth for orthodontic reasons were included in this study. Following the surgical procedure, the exposed pulp tissue was treated either with calcium silicate or covered with calcium hydroxide paste. On day 3, 7, 14 and 28, the experimental teeth was extracted and examined using light microscopy and histometric analysis to observe the inflammatory changes and the amount of reparative dentin formation. The results showed that in the calcium silicate treated teeth, substantial amounts of dentine-like tissue was formed on day 14 and mostly located on the exposure site. It was also observed in the calcium hydroxide treated teeth but dentin-like tissue located at a distance from the exposure site. The total amount of reparative dentine formed in the calcium silicate-treated teeth was significantly higher (p<0.005) than in the calcium hydroxide-treated specimens. In conclusion that the calcium silicate indices pulpal wound healing and reparative formation in the exposed teeth without affecting the normal function of the remaining pulp.

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