scholarly journals Influence of the Neural Crest Derived Stem Cells to Optic Nerve Regeneration After Its Experimental Injury

2021 ◽  
Keyword(s):  
Stem Cells ◽  
Optic Nerve ◽  
Soft Tissue ◽  
Neural Crest ◽  
Orbital Trauma ◽  
Tissue Content ◽  
Group I ◽  
Group Ii ◽  
Experimental Injury ◽  
Orbital Soft Tissue

Purpose: Orbital trauma is a challenging problem due to such severe sequel as diplopia, decrease of vision or eye motility disorder. However, the conditions of orbital soft tissue content still become underestimated. The aim of this study was to investigate structural changes in the rat optic nerve after experimental injury followed by treatment with stem cells. Materials and Ьethods: An experimental model of injury to the orbital soft tissue content in the rat was developed. Forty Wistar rats maintained under daylight were divided into two equal experimental groups. Unlike the rats of Group I, in rats of Group II, the site of injury to the orbital soft tissue mass received postnatal multipotent stem cells, epidermal neural crest stem cells (NCSCs) derived from the bulge of hair follicles. Results: Comparing the number of glial cells per certain area of the slice (NC) between group І and site without injury (control) after 3 week of observation, it was higher in group I more than 258.8% (p < 0.0001) and on 272.4% in group II (p < 0.0001). After 6 weeks NC in group I was higher than at previous terms: more then 128.9% (р < 0.0001). At the same, NC in group II was higher comparing with previous terms only on 17.1% (р = 0.0212). Between the animals of group I at terms of 12 and 24 weeks NC high and wasn’t significantly differ between this terms of observation (ANOVA p = 0.4379). In contrast, NC in group II stopped rising between 6 and 12 weeks demonstrating statistical equality (p = 0.4563). Conclusions: It can be assumed that the application of mesenchymal stem cells, derivates of the neural crest, after the experimental orbital trauma, stimulates a recovery of the optic nerve. Further studies should be performed to more deeply discover the neural crest derived stem cell populations, ivoleved into recovery of damaged optic nerves.

scholarly journals Influence of Adult Neural Crest-Derived Multipotent Stem Cells on Regeneration of Orbital Soft Tissue Content After Experimental Injury

2018 ◽  
Vol 28 (1) ◽  
pp. 059-063
Author(s):  
Yuri V. Chepurnyi ◽  
◽  
T. V. Kustrjo ◽  
Alina V. Korsak ◽  
Volodymyr V. Likhodievskyi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Soft Tissue ◽  
Neural Crest ◽  
Multipotent Stem Cells ◽  
Tissue Content ◽  
Experimental Injury ◽  
Orbital Soft Tissue

Morphological changes in the optic nerve after experimental injury followed by treatment with stem cells

2017 ◽  
Vol 69 (5) ◽  
pp. 50-55 ◽  
Author(s):  
I. Chepurnyi ◽  
◽  
A. Kopchak ◽  
A. Korsak ◽  
V. Likhodievskyi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Optic Nerve ◽  
Morphological Changes ◽  
Experimental Injury

scholarly journals Effects of doxorubicin associated with amniotic membrane stem cells in the treatment of canine inflammatory breast carcinoma (IPC-366) cells

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Jéssica Borghesi ◽  
Sara Caceres ◽  
Lara Carolina Mario ◽  
Angela Alonso-Diez ◽  
Ana Carolina Silveira Rabelo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Amniotic Membrane ◽  
Triple Negative ◽  
Cellular Growth ◽  
Control Group ◽  
Group Iii ◽  
Chemotherapy Drugs ◽  
Group I ◽  
Group Ii

Abstract Background Tumours in mammary glands represent the most common neoplasia in bitches, as in humans. This high incidence results in part from the stimulation of sex hormones on these glands. Among mammary tumours, inflammatory carcinoma is the most aggressive, presenting a poor prognosis to surgical treatment and chemotherapy. One of the most widely used chemotherapy drugs for breast cancer treatment is doxorubicin (DOXO). Alternative therapies have been introduced in order to assist in these treatments; studies on treatments using stem cells have emerged, since they have anti-inflammatory and immunomodulatory properties. The aim of this study was to evaluate the effects of DOXO and canine amniotic membrane stem cells (AMCs) on the triple-negative canine inflammatory mammary carcinoma cell line IPC-366. Methods Four experimental groups were analysed: a control group without treatment; Group I with DOXO, Group II with AMC and Group III with an association of DOXO and AMCs. We performed the MTT assay with DOXO in order to select the best concentration for the experiments. The growth curve was performed with all groups (I-III) in order to verify the potential of treatments to reduce the growth of IPC-366. For the cell cycle, all groups (I-III) were tested using propidium iodide. While in the flow cytometry, antibodies to progesterone receptor (PR), estrogen receptor (ER), PCNA, VEGF, IL-10 and TGF-β1 were used. For steroidogenic pathway hormones, an ELISA assay was performed. Results The results showed that cells treated with 10 µg/mL DOXO showed a 71.64% reduction in cellular growth after 72 h of treatment. Reductions in the expression of VEGF and PCNA-3 were observed by flow cytometry in all treatments when compared to the control. The intracellular levels of ERs were also significantly increased in Group III (4.67% vs. 27.1%). Regarding to the levels of steroid hormones, significant increases in the levels of estradiol (E2) and estrone sulphate (S04E1) were observed in Groups I and III. On the other hand, Group II did not show differences in steroid hormone levels in relation to the control. We conclude that the association of DOXO with AMCs (Group III) promoted a reduction in cell growth and in the expression of proteins related to proliferation and angiogenesis in IPC-366 triple-negative cells. Conclusions This treatment promoted ER positive expression, suggesting that the accumulated oestrogen conducted these cells to a synergistic state, rendering these tumour cells responsive to ERs and susceptible to new hormonal cancer therapies.

scholarly journals Obstetrical Soft Tissue Trauma during Spontaneous Vaginal Birth in the Romanian Adolescent Population—Multicentric Comparative Study with Adult Population

2021 ◽  
Vol 18 (21) ◽  
pp. 11491
Author(s):  
Alexandra Matei ◽  
Elena Poenaru ◽  
Mihai Cornel Traian Dimitriu ◽  
Cristina Zaharia ◽  
Crîngu Antoniu Ionescu ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Vaginal Birth ◽  
Adult Women ◽  
Young Mothers ◽  
Soft Tissue Trauma ◽  
Chi Square ◽  
Perineal Lacerations ◽  
Group I ◽  
Group Ii ◽  
Tissue Trauma

Romania is a country with high rates of adolescent births, associating scarce comprehensive obstetrical management with this specific population. This research aims to assess soft tissue trauma after vaginal birth in teenage mothers compared to their adult counterparts. A retrospective case-control study was conducted for one year in two hospitals. All vaginal deliveries were considered; the age cut-off value was considered at 20 years old for case and control groups. Lacerations were divided into three subgroups, considering the involved anatomical region; group I: labial and periurethral lacerations, group II: vaginal and perineal lacerations, and group III: cervical lacerations. There were 1498 women included in the study: 298 young mothers and 1200 adults. Teenagers were more likely to have an episiotomy during vaginal delivery compared to adult women: 56% versus 26.7% (p = 0.00, Pearson Chi-square) and a 1.89 times increased risk for developing additional group II lacerations: p = 0.01, Pearson Chi-square test with Bonferroni correction: OR = 1.89, 95% CI: 1.18–3.02. Group II lacerations were the most frequent type of birth trauma in both study groups. Fetal weight ≥4000 g was associated with a two times higher risk for vaginal and perineal lacerations when age criterion was not considered (OR = 1.98, 95% CI: 1.13–3.47, p = 0.01). The incidence of group I and II lacerations increased with age: from 0% and 9.1% between 10 and 14 years old to 6% and 26.2% between 18 and 19 years old. All groups of lacerations were more often identified in the case group, compared to the adult group. Fetal macrosomia and spontaneously ruptured membranes at admission could not be documented as risk factors for obstetrical injury in young mothers. Episiotomy performed in teenagers was not a protective procedure for group II lacerations.

Comparison of AdvanSync2® and Twin Block Appliances in Treatment of Class II Malocclusion With Retrognathic Mandible—An Observational Retrospective Study

2021 ◽  
pp. 030157422110572
Author(s):  
Ankit Kumar Shahi ◽  
Payal Sharma ◽  
Achint Juneja ◽  
Divya Shetty ◽  
Rishibha Bhardwaj ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Class Ii ◽  
T Test ◽  
Growth Spurt ◽  
Class Ii Malocclusion ◽  
Twin Block ◽  
Group I ◽  
Tissue Changes ◽  
Group Ii ◽  
Soft Tissue Changes

Objectives: To evaluate the treatment outcomes between Twin Block and AdvanSync2® appliances by comparing the skeletal, dentoalveolar, and soft tissue changes. Materials and Methods: Radiographic data of 20 patients were retrospectively analyzed. Data were selected from patients in their skeletal growth spurt as evaluated by the cervical vertebral maturation method (CVMI 2, 3, and 4), with class II malocclusion characterized with retrognathic mandible (ANB > 4°, SNB < 77°, FMA = 25 ± 5°, overjet > 5 mm). There were 10 patients in each group that underwent orthodontic correction for class II malocclusion: either using Twin Block or AdvanSync2®. Independent t test and Paired t test and chi-square tests were used for the data analysis. The level of statistical significance was set at P value ≤.05. Results: The chronological and skeletal age were similar in both the groups. Records were taken for the functional treatment with mean treatment span of 8 ± 1 month. Changes in SNB (group I = 1.59°, group II = 3.11°) ( P < .01), Co-Gn (group I = 2.89 mm, group II = 5.34 mm), and U1-L1° (group I = −1.51°, group II = 2.97°) showed statistically different outcome between the groups, when the pre-post data were studied. Rest of the variables—cranial base, maxillary skeletal, mandibular skeletal, intermaxillary, vertical skeletal, maxillary dentoalveolar, mandibular dentoalveolar, and soft tissue—showed similar outcome ( P > .05). Conclusion: Both appliances lead to desirable outcomes in the correction of class II malocclusion. AdvanSync2® resulted in inducing more of changes in SNB and effective mandibular length as compared to Twin Block. Overjet and molar relation improved significantly with both the appliances. Both the appliances resulted in similar skeletal, dentoalveolar, and soft tissue changes.

Comparative Study on the Effect of Adipose tissue derived Mesenchymal Stem Cells versus Metformin on Age-related Structural Changes of the Cerebellum in Albino Rat

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
M. M Doaa Karem ◽  
Soheir K Ahmed ◽  
Hanan A Saleh ◽  
Ghada Galal Hamam
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Purkinje Cells ◽  
Structural Changes ◽  
Female Rats ◽  
Group Iii ◽  
Age Related ◽  
Group I ◽  
Group Ii

Abstract Introduction Aging is a normal physiological process that affects all organs in the body including the cerebellum. Metformin is an anti-diabetic drug that is used in some age-related diseases. Regenerative medicine using adipose tissue derived mesenchymal stem cells (ADMSCs) is an emerging promising strategy. Aim to compare between the role of ADMSCs and metformin on the age-related structural changes of the cerebellum in female albino rats. Materials and methods Fifty-five female rats of different ages (4, 12 and 24 months) were included in this study. They were divided into three groups according to their ages: Group I (Adult rats), Group II (Old rats) and Group III (Senile rats). Group II and Group III were subdivided into three subgroups, Subgroup a: rats were left without treatment, Subgroup b: rats were given a single dose of 1X106 ADMSCs via tail vein. Subgroup c: Rats received300 mg/kg metformin/day orally. Rats were sacrificed after four weeks. The cerebellum was collected and processed for H&E, Toluidine blue and immuno-histochemical reaction using glial fibrillary acidic protein (GFAP). Results Histological examination of the cerebellum of the subgroups IIa and IIIa revealed age-related structural changes in comparison to group I. Purkinje cells appeared distorted with irregular outline. Some Purkinje cells were seen shrunken while others appeared ballooned. Focal loss of Purkinje cells was also noticed. Granular layer contained small widely separated granule cells. GFAP reaction revealed an apparent decrease in number of astrocytes and their processes. The structural changes were more obvious in subgroup IIIa. In ADMSCs treated subgroups (IIbandIIIb); more noticeable improvement of these changes was noticed compared to the corresponding metformin treated subgroups (IIc and IIIc). Conclusion ADMSCs was more effective than metformin in preventing some age-related structural changes of the cerebellum.

Surgical Treatment of Moderate Hallux Valgus: A Comparison of Distal Chevron Metatarsal Osteotomy With and Without Lateral Soft-Tissue Release

2017 ◽  
Vol 10 (6) ◽  
pp. 524-530 ◽  
Author(s):  
Maki Grle ◽  
Goran Vrgoc ◽  
Ivan Bohacek ◽  
Vladimir Hohnjec ◽  
Marko Martinac ◽  
...  
Keyword(s):  
Patient Satisfaction ◽  
Surgical Treatment ◽  
Soft Tissue ◽  
Hallux Valgus ◽  
Soft Tissue Release ◽  
Metatarsal Osteotomy ◽  
Group I ◽  
Group Ii ◽  
Surgical Treatments

Purpose: The purpose of the study was to determine whether lateral soft-tissue release (LSTR) has a beneficial or detrimental effect on the outcome of distal Chevron first metatarsal osteotomy (DCMO) in the treatment of moderate hallux valgus (HV). Methods: We compared the effect of different surgical treatments in 2 groups of patients: group I (23 patients, 25 feet, average age of 55 [from 43 to 77] years) was subjected to DCMO only, whereas group II (18 patients, 23 feet, average age of 59 [from 52 to 70] years]) was subjected to DCMO with LSTR. The American Orthopaedic Foot and Ankle Society’s Hallux Metatarsophalangeal-Interphalangeal scale survey was conducted postoperatively, followed by the brief survey on postoperative patient satisfaction. The patient follow-up period was from 18 to 24 months after surgical treatment, on average. Results: After surgical intervention, both groups of patients presented with an improved HV angle, but there was no significant difference between the groups. However, group II showed significant improvements in medial sesamoid bone position and patient satisfaction scores as compared with group I. Conclusion: Our midterm follow-up of surgical treatments for moderate HV deformity suggests that both procedures provide good postoperative results. However, according to our results, DCMO with LSTR provides better results than procedures without LSTR. Levels of Evidence: Therapeutic, Level III: Retrospective comparative study

scholarly journals AB1310-HPR EFFECTS OF INSTRUMENT-ASSISTED SOFT TISSUE MOBILIZATION ON FROZEN SHOULDER: A RANDOMIZED CONTROLLED TRIAL

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1945.2-1945
Author(s):  
E. Kaya Mutlu ◽  
T. Birinci ◽  
S. Kilic
Keyword(s):  
Soft Tissue ◽  
Range Of Motion ◽  
Frozen Shoulder ◽  
Controlled Study ◽  
Joint Mobilization ◽  
Randomized Controlled ◽  
Constant Murley Score ◽  
Group I ◽  
Group Ii ◽  
Soft Tissue Mobilization

Background:Frozen shoulder has a greater incidence, more severe course, and resistance to treatment in patients. Management is based on the underlying cause of pain and stiffness. Joint mobilization has been reported to improve joint range of motion in frozen shoulder. However, there is no information regarding the effect of instrument-assisted soft tissue mobilization (IASTM) in frozen shoulder. We proposed that there would be no significant difference between the two manual physical therapy techniques with relatively similar treatment effects in the frozen shoulder.Objectives:The aim of this randomized controlled study was to compare the effectiveness of IASTM and joint mobilization in the treatment of patients with frozen shoulder.Methods:Thirty patients with phase II frozen shoulder (mean age 50,9 years, age range 39–65 years) were randomly assigned to one of the two treatment groups: Group I received joint mobilization combined with manual stretching exercise and Group II received IASTM with manual stretching exercise (two days per week for six weeks) (Figure 1). The pain level was evaluated with a visual analogue scale (VAS) and the active range of motion (ROM) was measured with a universal goniometer. The Disabilities of the Arm, Shoulder and Hand score and the Constant-Murley score were used for functional assessment. The assessments were performed at baseline and after the 6-week intervention.Figure 1.Instrument-Assisted Soft Tissue MobilizationResults:Both groups had a significant decrease in pain according to VAS and a significant increase in ROM and function level (p<0.05). After the 6-week intervention, improvement of shoulder abduction ROM in Group I was found significantly higher than Group II (p=0.01), on the other hand, Constant-Murley score in Group II was found significantly higher compared to Group I (p=0,001).Conclusion:Our results supported the hypothesis that either joint mobilization or IASTM, performed in addition to stretching exercise, provided similar improvements in pain levels in patients with the frozen shoulder.References:[1]Kelley MJ, Shaffer MA, Kuhn JE, Michener LA, Seitz AL, Uhl TL, et al. Shoulder Pain and Mobility Deficits: Adhesive Capsulitis, J Orthop Sports Phys Ther, 2013:43:1-31.[2]Celik D, Kaya Mutlu E.Does AddingMobilization toStretchingImprove Outcomes for People with Frozen Shoulder? A Randomized Controlled Clinical Trial, Clin Rehab, 2016: 30(8): 786-794.[3]Iked N, Otsuka S, Kawanishi Y, Kawakami Y. Effects of Instrument-Assisted Soft Tissue Mobilization on Musculoskeletal Properties, Med Sci Sports Exerc, 2019:51(10): 2166-2172.Disclosure of Interests:None declared

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