scholarly journals The antibacterial effect of β-pinene derived from Citrus aurantifolia against oral Streptococcus mutans

2021 ◽  
Vol 33 (1) ◽  
pp. 88
Author(s):  
Euis Julaeha ◽  
Tati Herlina ◽  
Mohamad Nurzaman ◽  
Tri Mayanti ◽  
Dikdik Kurnia ◽  
...  
Keyword(s):  
Essential Oil ◽  
Streptococcus Mutans ◽  
Antibacterial Effect ◽  
Oral Disease ◽  
Molecular Formula ◽  
Citrus Aurantifolia ◽  
Antibacterial Compound ◽  
Oral Streptococcus ◽  
Quasi Experimental ◽  
Oral Microbes

Introduction: Streptococcus mutans has been known to play a major role in dental caries development. This tooth decay is the most common oral disease affecting people in the world. Hence, discovering the new herbal antibacterial agent seems to become promising yet challenging.  One of herbal antibacterial source is Jeruk Nipis or namely Citrus aurantifolia, as it is believed to contain biological active compounds that may act as antibacterial to kill pathogenic oral microbes. This study was aimed evaluated antibacterial effect  of β-pinene derived from Citrus aurantifolia against oral Streptococcus mutans. Methods: Type of research is quasi experimental research. The essential of C. aurantifolia essential oil was prepared and isolated using the hydro-distillation technique and further isolation of antibacterial compounds was conducted by combination column chromatography using organic solvent, and the structure was determined by infrared (IR), 1D NMR of 1H-, 13C-NMR and DEPT 135° in CDCl3, and UV-Vis spectrometer methods. The antibacterial activity was tested against Streptococcus mutans using the Kirby-Bauer method. Results: 0.75% yield was extracted from the essential oil of Citrus aurantifolia and after purification, an antibacterial compound was identified as β-pinene with the molecular formula C10H16. Furthermore, the Citrus aurantifolia exerted inhibition growth of Streptococcus mutans at concentration of 2000, 1000, and 500 ppm showed reduction in paper disk as much as 13.0, 11.9, and 11.6 mm respectively. Conclusion: Antibacterial effect  of β-pinene derived from Citrus aurantifolia against oral Streptococcus mutans. This is proven that β-pinene derived from Citrus aurantifolia prospective as antibacterial compound that potentially can be used as another herbal antibacterial of choice to manage dental disease associated with Streptococcus mutans infection.

scholarly journals Formulation of mouth rinse from the essential oils of lime (Citrus aurantifolia) and its inhibitory efficacy on the growth of Streptococcus mutans – in vitro

2020 ◽  
Vol 32 (1) ◽  
pp. 39
Author(s):  
Sri Mulyanti ◽  
Dewi Sodja Laela ◽  
Euis Julaeha ◽  
Anne Agustina Suwargiani ◽  
Dudi Aripin
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Essential Oils ◽  
Streptococcus Mutans ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Oil Quality ◽  
Mouth Rinse ◽  
Citrus Aurantifolia

Introduction: Essentials oil of lime (Citrus aurantifolia) peel has high activity in inhibiting the growth of Streptococcus mutans bacteria. The objective of this research was to determine the formulation of mouth rinse from the essential oils of lime (C. aurantifolia) with the highest inhibitory and on the growth of S. mutans. Methods: This research was an experimental in-vitro study. The sample used was the lime (C. aurantifolia) peel waste peel using hydrodistillation technique; testing of the essential oil quality was performed using 100°C distilled water for 3 hours; organoleptic test and physical mouth rinse formula test was also conducted. Mouth rinse formulations were made from the essential oils in the concentrations of 1, 2, 4, 8, 10, and 20%. Mouth rinse activity was tested with optical density. Minimum inhibitory concentration was determined with microdilution. Results: Essential oil mouth rinse formulation showed antibacterial activity at the concentrations of 2, 4, 8, 10, and 20% (formulations I-IV); the recommended mouth rinse concentration was 4% (formulation III) due to its highest antibacterial. Formulation IV showed the highest minimum inhibitory concentration value of 5.2 mg/mL (0.52%) and the safe minimum bactericidal concentration of 0.4 mg/mL (1.04). Conclusion: Mouth rinse formulation from essential oils of lime (C. aurantifolia) peel begins to show inhibition of the growth of S. mutans bacteria at the concentration of 2% essential oils (formulation II), with inhibition against the growth of S. mutans is 7.08. The formula shows a minimum inhibitory concentration (MIC) value and a minimum bactericidal concentration (MBC) of 5.2 mg/mL (0.52%) and 10.4 mg/mL (1.04), respectively.

scholarly journals Activity of Cloves, Cinnamon and Thyme Essential Oils Against Some Oral Bacteria

2021 ◽  
Vol 05 (1) ◽  
pp. 14-25
Author(s):  
Zainab AL-Mahdi ◽  
Luma Witwit ◽  
Israa Ubaid
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Essential Oils ◽  
Streptococcus Mutans ◽  
Antibacterial Effect ◽  
Inhibitory Effect ◽  
Oral Bacteria ◽  
Management Approach ◽  
Thyme Essential Oil ◽  
Lactobacillus Spp

Background: Gastronomic natural flavors and spices have been used as both flavoring agents and preservatives in food preparation for many years. They have also been utilized in medical field for their biological properties shown in previous studies. Objective: The study aimed to evaluate the inhibitory effect of the oil extracts from Cloves, Thyme, and Cinnamon compared with ampicillin on some oral bacteria (Staphylococcus aureus, Streptococcus mutans, Lactobacillus spp., Pseudomonas aeruginosa, and Proteus spp.). Method: Evaluation of the antibacterial effect of herbal and spices essential oil was performed using Agar well diffusion test. Its effect was assessed against oral bacteria, which diagnosed in laboratory by culturing on enriched and selective media and by biochemical test. Results: High inhibitory effect of cinnamon, clove against some Gram positive and Gram negative isolated oral bacteria (Staphylococcus aureus, Streptococcus mutans, Lactobacillus spp., Pseudomonas aeroginosa, and Proteus spp. comparing with ampicillin. While thyme essential oil shows inhibitory effect for the bacterial isolates except for Pseudomonas aeroginosa but less than the inhibitory effects of other essential oils and ampicillin. For Psedomonas aeruginosa result shows the bacteria have resistance to ampicillin and Thyme essential oil while both cloves and cinnamon have antibacterial effect. Conclusions: Cloves, thyme and cinnamon essential oil have a wide antimicrobial activity against some oral bacteria, both cinnamon and cloves essential oils recorded superior antibacterial activity than ampicillin for (Proteus spp and lactobacillus), competence action with ampicillin for Staphylococcus aureus and Streptococcus mutans and novel antibacterial action against Pseudomonas aeroginosa which resist to ampicillin which may be a promising antibacterial management approach and resist development of antibiotic resistance strain if used instead than the antibiotic. Also, suggestion for using these oils orally may provide a degree of protection in oral cavity against some oral pathogens.

Evaluation of antibacterial efficacy of Triphala toothwipes on oral Streptococcus mutans count in intellectually disabled children

2021 ◽  
Author(s):  
Meghana Ajay Deshpande ◽  
Sudhindra Baliga ◽  
Nilima Thosar ◽  
Nilesh Rathi ◽  
Shriramji Jyothishi ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Antibacterial Efficacy ◽  
Disabled Children ◽  
Intellectually Disabled ◽  
Oral Streptococcus

scholarly journals Synergistic antibacterial combination of Lavandula latifolia Medik. essential oil with camphor

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Nursenem Karaca ◽  
Görkem Şener ◽  
Betül Demirci ◽  
Fatih Demirci
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Antibacterial Effect ◽  
Synergistic Effects ◽  
Pharmaceutical Formulations ◽  
Human Pathogens ◽  
Broth Microdilution ◽  
Antibacterial Effects ◽  
Lavandula Latifolia

AbstractCombination of various compounds and essential oils for pharmaceutical formulations withdraw attention. In this present study, it was aimed to evaluate the in vitro potential synergistic antibacterial effect of Lavandula latifolia (spike lavender) essential oil with camphor by using the checkerboard method against the human pathogens; Staphylococcus aureus and Listeria monocytogenes. Pharmacopoeia quality L. latifolia essential oil and racemic camphor were analyzed and verified by GC-FID and GC/MS, simultaneously. In vitro antibacterial activity of essential oil and camphor (MIC range: 0.16–20 mg/mL) and standard antimicrobial clarithromycin (MIC range: 0.125–16 μg/mL) were carried out by broth microdilution against S. aureus and L. monocytogenes standard strains, respectively. Resulting antibacterial effects were evaluated for their fractional inhibitory concentrations (FICs) as antagonistic, additive and synergistic effects. The analytical results showed that the major component of essential oil was linalool (45.2%) and 1,8-cineole (25.6%). Antibacterial effects of essential oil were determined as MIC 1.25–5 mg/mL. As a result of the experiments, L. latifolia essential oil–camphor combinations were identified as “synergistic (FIC ≤ 0.5), and additive (0.5 < FIC ≤ 1)” in the respective combinations, suggesting further evaluation for formulations for potential antimicrobial applications in food and pharmaceuticals.

Enzyme‐assisted extraction of essential oils from Syzygium aromaticum

2011 ◽  
Vol 1 (6) ◽  
pp. 248-254
Author(s):  
R. Amudan ◽  
D. V. Kamat ◽  
S. D. Kamat
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Steam Distillation ◽  
Antibacterial Effect ◽  
Chemical Extraction ◽  
Syzygium Aromaticum ◽  
Clove Oil ◽  
Zone Size ◽  
Structure Quality ◽  
Assisted Extraction

The essential oil of clove ( Syzygium aromaticum) holds an important positionamongst widely used essential oils. A typical steam distillation processfor the extraction of clove oil provides a 10.1% yield. Recent studies involvedthe use of enzymes such as pectinase, amylase, lignocellulase, and cellulaseon the powder of clove buds, prior to extraction. The traditional methods ofphysical and chemical extraction are effective but may affect the structure,quality and yield of the phytochemicals extracted. In the current study,hence, enzymes specific for action on the cell wall have been used in the pretreatment prior to extraction, to enhance the quality and yield of the phytochemicalsextracted. The results indicated that all the enzymes, gave morethan 50% higher yield than control in terms of weight of extracted essentialoil. A mixture of the enzymes gave the highest yield of 17.82%. Gas chromatographyresults indicated that the essential oil extracted using amylase hada maximum eugenol content of 70%, in comparison with the eugenol content(62–68%) in the essential oils extracted using the rest of the enzymes.Antibacterial activity of all the extracts was studied on methicillin â€resistantStaphylococcus aureus  (MRSA). The essential oil extracted by using amylaseinhibitedMRSA, showed a zone size of 40 mm, whereas the essential oil extractedby using lignocellulase showed a zone size of 45 mm. The gas chromatogramindicated the maximum number of peaks in this extract, whichcould be producing a combined antibacterial effect on the organism. Thespecific gravity values of the essential oil extracted using lignocellulase andamylase was 1.051 and 1.062, respectively, whereas the control had a specificgravity of 1.015.

Salivary mucin as related to oral Streptococcus mutans in elderly people

2000 ◽  
Vol 15 (1) ◽  
pp. 10-14 ◽  
Author(s):  
L. W. Baughan ◽  
F. J. Robertello ◽  
D. C. Sarrett ◽  
P. A. Denny ◽  
P. C. Denny
Keyword(s):  
Elderly People ◽  
Streptococcus Mutans ◽  
Salivary Mucin ◽  
Oral Streptococcus
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