scholarly journals The potential of cassava leaves extract in gingival fibroblasts of periodontitis of rats with ovarian dysfunction

2021 ◽  
Vol 33 (3) ◽  
pp. 226
Author(s):  
Amandia Dewi Permana Shita ◽  
Zahara Meilawaty ◽  
Dhesyarmani Putri Rothschildi ◽  
Agustin Wulan Suci Dharmayanti ◽  
Zahreni Hamzah

Introduction: The prevalence of periodontitis has reached 42.8% in Indonesia. One contributing factor is Porphyromonas gingivalis. The ovarian dysfunction caused by ovariectomy procedures may also cause periodontitis. Post ovariectomy conditions resemble menopause, lowering the production of estrogen and progesterone hormones, leaving patients vulnerable to periodontitis. To prevent the side effects of the treatment, herbal ingredients are commonly used. The extract of cassava leaves is known to have pharmacological effects as an antioxidant and, thus, can be applied as a medicine for periodontitis.      Methods: A total of 21 rats were divided into three groups, (1) control: healthy rats; (2) Group P.1: P. gingivalis-induced rats; (3) Group P.2: ovariectomized rats. Groups P.1 and P.2 were further divided into three subgroups that were administered cassava leaves extract at a dosage of 179.2 mg/ kg BW, aquades and vitamin C. Rats were euthanized at day eight after the initial treatment to collect left mandibular first molar. The mandibular sections were immunohistochemically stained to quantify SOD expression using light microscope while the Image J software was used. Fibroblast cells expressing SOD are characterized by brown coloration in the cytoplasm and darker nucleus. Results: In periodontitis conditions, either due to P.gingivalis induction or ovarian dysfunction, fibroblast cells in rats given cassava leaves extract expressed higher SOD than rats given aquades, but still lower than rats given vitamin C. The result of the one way ANOVA test showed p value of 0,00, which means that there is a significant difference in all groups. Conclusion: Cassava leaves extract demonstrated the potential to increase the antioxidant expression in P. gingivalis-induced and ovarian dysfunction rats.

2021 ◽  
Vol 2 (2) ◽  
pp. 142
Author(s):  
Syahrizal Syahrizal ◽  
Junaidi Junaidi

Background: Flies (Musca domestica) can act as vectors of typhus, dysentery, and cholera. Various methods have been used to control flies from around human life, either mechanically (light traps), a chemical in the form of toxic baits or insecticides, or with scents that are repellent. A mixture of curry leaf extract with soursop leaves can be used as an insecticide to repel house flies. Because curry leaves and soursop leaves contain compounds, tannins, saponins, and flavonoids that flies do not like.Objective: The purpose of this study was to determine the effectiveness of a mixture of curry leaves with soursop leaves as a natural insecticide in repelling the flies (Musca Domestica).Methods: This research is an experimental design with a mixture of curry leaf extract with soursop leaves on the power of repelling house flies. This research was conducted in 2019 in Darul Kamal District, Aceh Besar. The object of the research was 450 house flies, which consisted of five treatments with three repetitions. Data analysis was carried out with the one-way ANOVA statistical test.Results: The descriptive results showed that at a dose of 10gr/100ml the average flies were able to repel 37 flies, at a dose of 20 gr/100ml were 44, at a dose of 30 gr/100ml were 59 and at a dose of 40 gr/100ml was 70. Of the four treatments, the most effective dose was at a dose of 40gr/100ml, which was 70 (77.7%). Statistically, it showed that there was a significant difference (p < 0.05) between various doses/treatments of mixed extracts of curry leaves (Murraya koeginii) and soursop leaves (Annona muricata Linn) on the repelling power of house flies (Musca domestica), with p-value = 0.000.Conclusion: A mixture of curry leaf extract (Murraya koeginii) with soursop leaf (Annona muricata Linn) is effective as an insecticide in repelling house flies (Musca domestica).


2020 ◽  
Vol 20 (1) ◽  
pp. 59-64
Author(s):  
Eva Fitriana ◽  
Surahma Asti Mulasari

Latar Belakang: Lalat merupakan salah satu serangga yang memiliki populasi tinggi di lingkungan masyarakat. Lalat berperan dalam penularan penyakit pada manusia seperti disentri, tifoid, kolera infeksi cacing, conjungtivitis, trachoma kusta, mikosis, dan difteri kutaneus. Fly trap digunakan untuk pengendalian lalat dewasa dalam jumlah besar dan padat. Penerapan Fly trap dilakukan diluar rumah pada udara terbuka dan tidak terhindar dari pepohonan. Penggunaan alat ini berpasangan dengan umpan yang digunakan untuk menarik lalat dengan bau dan kandungan pada umpan. Penelitian ini dilakukan untuk mengetahui efektifitas variasi umpan pada Fly trap dalam pengendalian lalat di tempat pembuangan sementara (TPS) Jalan Andong Yogyakarta.Metode: Penelitian ini merupakan penelitian eksperimen dengan variasi umpan ikan, udang, buah nangka, terasi, dan buah mangga. Pengukuran dilakukan pada radius ± 1 Meter dari tumpukan sampah dan dibiarkan selama 2 jam selanjutnya lalat yang terperangkap dihitung menggunakan handcounter. Data yang diperoleh dianalisis menggunakan uji One Way Anova dengan derajat kesalahan α 0.05 dan dilanjutkan uji Post hoc Tamhanne.Hasil: Hasil penelitian menunjukan terdapat hasil yang signifikan rata-rata lalat yang terperangkap pada Fly trap dengan umpan terasi dan umpan udang diperoleh nilai p=0,042 (p<α). Terdapat perbedaan bermakna antara umpan terasi dan umpan udang. Lalat yang terperangkap pada Fly trap umpan terasi sebanyak 69 ekor lalat, dan lalat yang terperanfkap pada Fly trap umpan udang sebanyak 1123 ekor lalat.Simpulan: Ada perbedaan rata-rata lalat yang terperangkap pada Fly trap umpan ikan, udang, buah nangka, terasi dan buah mangga. Terdapat perbedaan bermakna rata-rata lalat yang terperangkap antara Fly trap umpan terasi dan umpan udang. ABSTRACT Title: The Effectivennes of various feed variations in the fly trap as a control of fly density at the Andong street Yogyakarta.Background: Flies are one of the insects that have a high population in the community. Flies play a role in transmitting disease to humans such a dysentery, typoid, cholera, worm infection, conjungtivitis, leprosy trachoma, mycosis and cutaneus diphteria. Fly traps are used fpr the control of large and dense adult flies. The application on fly traps is carried out outside the house in the open air and is not spared from trees. The use of this tool is paired with the bait used the attract flies to get closer to the smell and content of the bait, this research was conducted to determine the effectiveness of bait varians in the fly trap in controlling flies at the temporary disposal site (TPS) at the Andong street Yogyakarta.Method: This research was a experimental study with a variety of bait fish, shrimp, jackfruit, shrimp paste, and mango. Measurements were made at radius of ± 1 meter from the garbage pile. The data obtained were analyzed using the One Way Anova tesy with an error degree of α=0,05 and continued with the Post hoc Tamhanne test.Result: The result showed that there were significant result on the average of flies trapped in the fly trap with the shrimp paste bait and shrimp bait obtained p value = 0.042 (p<α). That there was a significant difference between shrimp paste bait and shrimp bait.Conclusions: There was a difference in the average number of flies trapped in fly traps for fish, shrimp, jackfruit, shrimp paste, mango. There was a significant difference of flies trapped between shrimp paste bait and shrimp bait. Fly trap shrimp bait is the most preferred bait by flies shrimp paste bait is the least preferred bait by flies.  


2019 ◽  
Vol 10 (2) ◽  
pp. 44-50
Author(s):  
Rinaldi Daswito ◽  
Rima Folentia ◽  
M Yusuf MF

One of the diseases that can be transmitted by flies is diarrhea. Green betel leaf contains essential oils, chavicol, arecoline, phenol, and tannins which function as plant-based insecticides. This study aimed to determine the effectiveness of green betel leaf extract (Piper betel) as a plant-based insecticide on the number of mortality of house flies (Musca domestica). The research was an experimental study used After Only Design used the One Way Anova test with a 95% confidence level. The samples used were 360 ​​house flies. Each treatment of 30 house flies with 4 repetitions and used three concentrations of green betel leaf extract (25%, 50%, 75%). The study was conducted at the Chemistry and Microbiology Laboratory of Health Polytechnic Tanjungpinang, while the location of the fly collection was at the Tokojo Garbage Collection Station in Bintan Regency. The number of mortality of house flies at a concentration of 25% was 81 heads (67.5%), 50% concentrations were 93 heads (77.5%), and at a concentration of 75% were 103 heads (85.83%). There was an effect of green betel leaf extract on the mortality of house flies (p-value 0.0001 <0.05) with the most effective concentration of 75%. Further research is needed to obtain a finished product utilizing green betel leaf extract as a vegetable insecticide, especially in controlling the fly vector. Need further research on the use of green betel leaf extract as a vegetable insecticide controlling the fly vector by taking into account the amount of spraying and the age of the fly.   Keywords: Green betel leaf extract , organic insecticide, houseflies


Micromachines ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 3
Author(s):  
Kyo-in Koo ◽  
Andreas Lenshof ◽  
Le Thi Huong ◽  
Thomas Laurell

In the field of engineered organ and drug development, three-dimensional network-structured tissue has been a long-sought goal. This paper presents a direct hydrogel extrusion process exposed to an ultrasound standing wave that aligns fibroblast cells to form a network structure. The frequency-shifted (2 MHz to 4 MHz) ultrasound actuation of a 400-micrometer square-shaped glass capillary that was continuously perfused by fibroblast cells suspended in sodium alginate generated a hydrogel string, with the fibroblasts aligned in single or quadruple streams. In the transition from the one-cell stream to the four-cell streams, the aligned fibroblast cells were continuously interconnected in the form of a branch and a junction. The ultrasound-exposed fibroblast cells displayed over 95% viability up to day 10 in culture medium without any significant difference from the unexposed fibroblast cells. This acoustofluidic method will be further applied to create a vascularized network by replacing fibroblast cells with human umbilical vein endothelial cells.


2021 ◽  
Vol 2 (2021) ◽  
pp. 28-39
Author(s):  
Dimitar Avramov ◽  

The aim of this study was to determine aerobic fitness through the VO2max treadmill test of elite Bulgarian taekwondo players with international results, and to determine whether the aerobic system had an effect upon the sports result in taekwondo. Fourteen elite taekwondo athletes, members of the Bulgarian national team (8 male and 6 female) were tested using a continuous progressive treadmill test. Physiological characteristics such as maximal oxygen uptake(VO2max), blood lactate and heart rate were measured. The male athletes recorded 58.2±3.4 ml kg–1 min–1 and the female 46.0±2.8 ml kg–1 min–1. The lactate level reached its highest at the 6’ after the VO2max with results for the males of 11.5±3.7 (mmol l-1) and 9.9±4.1 (mmol l-1) for the females respectively. A comparison between our results, regarding VO2max and previously reported was made using the One-way Anova for independent samples. It showed no significant difference between the male subjects (58.2±3.4 versus 60.7±3.3 ml kg(-1) min(-1), p>.05) and significant difference between the female ones (46.0±2.8 versus 49.8±2.8 ml kg(-1) min(-1), p<.05). Investigated also was the number of kicks executed by the winner of -49 kg weight category and her direct opponents during the 2019 Grand Prix Sofia. It was discovered that the winner kicked an average of 86.25 times per match and her kicks during the Grand Prix Sofia accumulated to 390 in total. It is our conclusion that the aerobic fitness does not play a significant role in taekwondo.


2017 ◽  
Vol 11 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Saja Ali Muhsin

Background: Although bonding to denture teeth after surface treatment with chemical agents is desirable, there is little information on the use of Visible Light Cure composite resin (VLC) as bonding denture materials. Objectives: To determine the effect of various surface treatments on shear bond strength between Visible Light Cure composite resin and the acrylic denture teeth interface. Methods: Forty cylindrical sticks of acrylic resin with denture teeth mounted atop were prepared. Various treatments were implemented upon the acrylic resin teeth surfaces. The samples were divided into four groups (n = 10). Light-cured composite resin (LC) was applied over all treated and untreated surfaces of tested groups. The shear bond was tested using a universal tensile testing apparatus with the knife-edge of a 0.8mm shear tester. Data were statistically analyzed using one-way ANOVA performed at a confidence level of 95% and significant P-value of (P ≤ 0.05). Results: Analysis of variance (ANOVA) showed statistically significant difference (P < 0.05) between treated and untreated teeth surfaces. The treated surfaces exhibited various levels of bond strength depending on the type of treatment. Conclusion: Application of VLC bonding agent with prior treatment of methylmethacrylate (MMA) on the acrylic resin denture teeth resulted in maximum bond strength with composite resin.


2019 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Maria Rosario Catacutan

Purpose This study aims to investigate attitudes toward cheating among business students at a private university in Kenya and examine if a significant difference exists in cheating perceptions among students who have completed one or two ethics courses, and those who have done none. Design/methodology/approach A total of 554 undergraduate business students participated in this research. Data were analyzed using descriptive statistics and the one-way ANOVA. Findings The results found that students perceived cheating in exam-related situations as quite serious, while cheating on written assignments was not considered a serious offence. Results of the one-way ANOVA indicate that there was a significant difference in the cheating perceptions ratings for the three groups. Post hoc comparisons using the Tukey HSD test indicate that the mean score for students who have done two ethics courses was significantly different from that of students who have done only one ethics course. Practical implications This study has a number of implications for educators and administrators. Ethics instruction cannot achieve its desired effect on student behavior without institutional support. Administrators also need to be cognizant of the influence that school environment has on student cheating. Faculty and university administrators can influence students’ behavior in the way they practice academic integrity in their teaching and administrative functions. Originality/value To the best of the author’s knowledge, this research is the first study to explore academic cheating at a private Kenyan university where ethics instruction is taught to undergraduate students.


2020 ◽  
Vol 16 (12) ◽  
pp. 1088-1093
Author(s):  
Mathew Thomas Maliael ◽  

It is of interest to establish the cephalometric correlation of angular data between frankfort horizontal and the sella-nasion line in different sagittal skeletal bases. Beta angle was used to divide the sample based on their sagittal skeletal base relationship. The FH-SN angle was measured for each group. The data were tabulated into IBM SPSS software. Kolmogorov-Smirnov and Shapiro-Wilk test was done to test the normal distribution of the data. One-way ANOVA analysis was done to test the difference of the FH-SN angle among the groups. Independent samples t-Test was done to test for gender dimorphism. The mean FH-SN angle of the sample was 6.33°3.35°. The results of the One-Way ANOVA and independent samples t-Test were insignificant. Results show that is no statistically significant difference in FHSN angle between skeletal class I, II and III.The mean FH-SN angle of the sample was 6.33°3.35°. The distribution of the data was normal. The results of the One-Way ANOVA and Independent samples t-Test were insignificant. There was no statistically significant difference in FH-SN angle between skeletal class I, II and III.


2020 ◽  
Vol 32 (2) ◽  
pp. 17-21
Author(s):  
Yasir Allabban

Background: The aim of this study is to evaluate the color change ∆E of the dental enamel following treatment with 2 kinds of protector (icon infiltrant, clinpro varnish) before fixed orthodontic treatment to avoid the possible white spot lesions. Materials and Methods: Fifty four subjects treated with fixed appliances were divided into 3 groups: the 1st group was control, while the 2nd and 3rd groups were treated with icon infiltrant and clinpro varnish before bonding procedure, respectively. Color parameters (L,a,b) were recorded for the middle and gingival thirds before and after bonding procedure to get the ∆E of each group. Results: One-way ANOVA test showed a non-significant difference in ∆E between the 3 groups at p value <0.05, while there were highly significant differences in value of ∆E between middle thirds and the gingival thirds in all groups. Conclusion: The icon infiltrant and clinpro varnish have no effect on color of the enamel when used before the bonding procedure. Color change ∆E occurring in middle third is higher than that of the gingival third. Keywords: Color change, protector, white spot lesions


Author(s):  
Abdul Halim Masnan ◽  
Muhammad Haziq Mohd Sharif ◽  
Masayu Dzainuddin ◽  
Mohd Mokhzani Ibrahim ◽  
Asmayati Yahaya ◽  
...  

<span>This study aimed to determine the professional identity concept based on professionalism requirement in Malaysia’s new preschool curriculum. Three professional identities identified through works of literature such as professional qualifications, experience and professional development were verified based on the constructed hypothesis to validate the concept of professional identity. The analysis findings showed a significant difference between professional qualifications. The same analysis, however, demonstrated that there was no significant difference on the preschool curriculum knowledge with regards to experience, but there was a significant indication of interaction between professional qualifications and experience on preschool curriculum knowledge. Meanwhile, for the one-way ANOVA test, there was a significant difference in the preschool curriculum knowledge based on professional development. The findings of this study confirmed that professional knowledge and professional development respectively hold a direct impact on the preschool curriculum, while experience acts as a support for professional qualifications.</span>


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