Relationship between antioxidant properties and GC-MS component composition of extracts from flowers, leaves and fruits of Crataegus Oxycanta

2015 ◽  
Vol 4 (1) ◽  
pp. 310-315
Author(s):  
Vera Hadjimitova
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Properties ◽  
Order Effect ◽  
Component Composition ◽  
Comparative Investigation ◽  
Antioxidant Effect ◽  
Model Systems ◽  
Ethanol Extracts ◽  
C 50 ◽  
H2o2 System

The aim of this study was to perform a comparative investigation of the antioxidant effect of Crataegus oxycanthas’ flowers, leaves, green and ripe fruits probes and to relate the obtained results with the ones of the accomplished qualitative GC-MS analysis of the antioxidant components participating in the studied standardized ethanol extracts. We established that the three extracts have well manifested antioxidant properties in three biological relevant model systems with different mechanism of generation of ROS: Fe-induced lipid peroxidation, UV induced deoxyribose damage and horse-radish peroxidase (HRP)-H2O2 chemiluminescent system. In the UV system the strongest effect is observed for the extract from flowers and leaves (C-50 = 0.109±0.009 mg/ml), followed by the ones from ripe fruits (C-50 = 0.259±0.015 mg/ml) and finally – by green fruits (C-50 = 0.557±0.014 mg/ml). In the Fe-induced lipid peroxidation system the antioxidant effect of the studied samples decreases in the following order: effect of flowers and leaves (C-50 = 0.287±0.018 mg/ml) > effect of green fruits (C-50 = 0.495±0.021 mg/ml) > effect of ripe fruits (C-50 = 0.704±0.035 mg/ml). Similar results were obtained in the HRP-H2O2 system. The generalized results show that the strongest antioxidant effect is observed for the plant extract from flowers and leaves of Crataegus. The GC-MS analyses we carried out indicate that the observed differences are due to the presence of a bigger number of constitutes possessing antioxidant properties in flowers and leaves extracts, compared with the fruits extracts.

Antioxidant Properties of Rimantadine in Influenza Virus Infected Mice and in Some Model Systems

2000 ◽  
Vol 55 (9-10) ◽  
pp. 824-829 ◽  
Author(s):  
Milka Mileva ◽  
Vera Hadjimitova ◽  
Lubka Tantcheva ◽  
Trayko Traykov ◽  
Angel S. Galabov ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Influenza Virus ◽  
Blood Plasma ◽  
Direct Action ◽  
Antioxidant Properties ◽  
Influenza Virus Infection ◽  
Antioxidant Effect ◽  
Model Systems ◽  
Prooxidant Effect

Abstract Influenza virus infection is associated with development of oxidative stress in lung and blood plasma, viz. increase of primary and secondary lipid peroxidation products. It was established that rimantadine treatment led to a decrease of the products of lipid peroxidation in tissues of mice experimentally infected with influenza virus A/Aichi/2/68 (H3N2). The effect is strongest in blood plasma (a decrease of about 50%) and weaker in the lung (about 20%). To elucidate the mechanism of this action of rimantadine, experiments were carried out with some model systems. The capability of rimantadine to scavenge superoxide radicals (scavenging properties) was studied in a system of xanthine-xanthine oxidase to generate superoxide. The amount of superoxide was measured spectrophotometrically by the NBT-test and chemiluminesce. Rimantadine does not show scavenging properties and its antioxidant effect observed in vivo, is not a result of its direct action on the processes of lipid peroxidation and/or interaction with antioxidant enzymes. The antioxidant properties of rimantadine were investigated by measurement of induced lipid peroxidation in a Fe2+ and (Fe2+ - EDTA) system with an egg liposomal suspension. Our findings with model systems do not prove an antioxidant or prooxidant effect of the drug on the processes of lipid peroxidation. Apparently, the observed antioxidant effect of rimantadine in vivo is not connected directly with free radical processes in the organism.

scholarly journals Antioxidant Properties of Phenolic Compounds from Baccharis articulata and B. usterii

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900
Author(s):  
Simone Quintana de Oliveira ◽  
Virgínia Demarchi Kappel ◽  
Viviane Silva Pires ◽  
Claiton Leoneti Lencina ◽  
Pascal Sonnet ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Antioxidant Effect ◽  
Trap Assay ◽  
Lead Compounds ◽  
Dicaffeoylquinic Acid ◽  
Total Antioxidant

As a continuation of our research on the Baccharis genus, the evaluation of the antioxidant effect by TRAP and TBARS assays of three isolated compounds from n-butanol fractions of B. articulata and B. usterii is reported. The structures of these compounds were established as 4- O-β-D-glucopyranosyl-3,5-dimethoxybenzyl-methanol (1), 5- O-[ E]-dicaffeoylquinic acid (2), and 7-hydroxy-5,4′-dimethoxyflavone (3). In the TRAP assay it was possible to observe an antioxidant effect of both n-butanol fractions at 1.25 μg/mL. Among the isolated compounds, compound 2 displayed a remarkable contribution to the total antioxidant capacity of the n-butanol fraction of B. usterii. Moreover, the n-butanol fractions of both species, compounds 1 and 2 at 40.0 μg/mL were efficient in protection of lipid peroxidation in the TBARS experiment. They are promising lead compounds for use in medicinal chemistry studies.

scholarly journals Evaluation of Toxicity and Antioxidant Property of Cassia fistula Stem Bark Extracts in Zebrafish

2020 ◽  
Vol 10 (03) ◽  
pp. 109-115
Author(s):  
Shreya Udaya ◽  
Nishith Babu ◽  
Dechamma Pandyanda Nanjappa ◽  
Krithika Kalladka ◽  
Gunimala Chakraborty ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Antioxidant Property ◽  
Stem Bark ◽  
Oxygen Species ◽  
Cassia Fistula ◽  
Potential Applications ◽  
Ethanol Extracts ◽  
Stress Models

Abstract Objective This study was aimed at evaluating the toxicity and the antioxidant property of Cassia fistula stem bark extracts in zebrafish. Materials and Methods Crude aqueous and ethanol extracts of C. fistula stem bark were obtained following a standard solvent-based extraction method. The toxicity of these extracts on zebrafish embryonic development was determined and the LC50 values were calculated. Finally, the antioxidant property of C. fistula stem bark extracts was determined in arsenic-induced oxidative stress models of zebrafish. Results The aqueous extract of C. fistula stem bark showed a slightly larger LC50 value (213.6 ppm) compared with the ethanol extract (LC50 = 63.5 ppm), suggesting a lower toxicity of the aqueous extract. A significant reduction of reactive oxygen species (ROS) signal was observed in arsenic-exposed embryos treated with the aqueous extract, but not the ethanol extract, indicating that the antioxidant activity is present only in the aqueous extract of C. fistula stem bark. Conclusion Identification of antioxidants from natural sources is desirable because of increasing safety concerns associated with synthetic antioxidants. This study demonstrated that aqueous extract from C. fistula stem bark possesses antioxidant properties, which can be further characterized for mechanism of action and potential applications.

Effect of cerium dioxide nanoparticles on certain indication of synthetic activity Rhodotorula glutinis

2020 ◽  
Vol 12 (2) ◽  
pp. 166-173
Author(s):  
Anastasiia Obradovych ◽  
Liliia Vasina ◽  
Nadiia Zholobak
Keyword(s):  
Cerium Dioxide ◽  
Culture Medium ◽  
Antioxidant Properties ◽  
Rhodotorula Glutinis ◽  
Component Composition ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Synthetic Activity ◽  
Yeast Saccharomyces Cerevisiae ◽  
Active Compounds

Cerium dioxide nanoparticles (CeO2 NP) have significant catalytic and antioxidant properties due to their ability to inhibit the development of free-radical reactions due to a combination of superoxidedismutase and catalase mimetic activities. This nanopreparation has a significant antibacterial effect, but is low-toxic to unicellular yeast Saccharomyces cerevisiae. Therefore, it is relevant and appropriate to study the effect of this compound on other groups of yeast, in particular members of the genus Rhodotorula spp., which are characterized by high growth rate, unpretentiousness to the component composition of the culture medium, the ability to form a variety of valuable biologically active compounds, as well as the ability to synthesize proteins, lipids, carotenoids and exopolysaccharides in the complex. The report that microorganisms of this genus have been identified as part of the normal fish microbiota (Abramis brama, Rutilus rutilus, Perca fluviatilis, Oreochromis niloticus) expands the range of their use, either as probiotics in general or their individual cellular and extracellular components as prebiotics. In this work, the effect of different concentrations of CeO2 NP nanopreparation on the production parameters of Rhodotorula glutinis was studied, as well as their antagonistic properties after saturation of nanocerium were tested. To detect the optimal concentration of nanopreparation of cerium dioxide, R. glutinis was cultured for 120 h at 28 0C and aerated at 160 rpm on Saburo medium with the addition of citrate-stabilized CeO2 NP with a particle size of 1-2 nm in the following concentrations: 100 mM, 10 mM, 1 mM, 0,1 mM, 0,01 mM. The control was R. glutinis grown on Saburo medium, experimental controls were represented by microorganisms in the culture medium of which citrate and full-size cerium (CeCl3) were added. It was found that most of the studied concentrations of CeO2 NP (except for the highest) are not toxic to these carotenogenic yeasts. The presence of 10 mM CeO2 NP stimulates the growth of the culture, while the increase in biomass is increased by fourfold compared to the control. There was also an increase in the content of basic metabolites – protein by 1,2 times, lipids by 2,5 times and valuable biologically active compounds - carotenoids by 20% and exopolysaccharides by 2,2 times. However, under these conditions, no significant changes in catalase and superoxidase activities were registered. Examination of the antagonistic properties of the CeO2 NP-enriched culture of R. glutinis by delayed antagonism by the method of perpendicular strokes against gram-positive B. subtilis and gram-negative P. syringae bacteria showed the presence of lysis zones up to 17 and 25 mm, respectively, in contrast to their complete or complete absence (2 mm), noted in the study of control samples. A study of the resistance of CeO2 NP-enriched culture of R. glutinis to natural inhibitors of the digestive tract showed resistance of microorganisms to gastric juice (the amount of CFU is 1,4 times higher than control values) and bile (recorded a 5-fold increase in survival of microorganisms). The obtained results testify to the prospects of using nanocerium for mobilization of growth, enzymatic, antagonistic activities of R. glutinis culture.

Phytochemical and antioxidant properties of Algerian Medicago Sativa L. Water, Ethenol and Chloroform extract

2019 ◽  
Vol 9 (3) ◽  
pp. 80-86
Author(s):  
Hanane Megdad ◽  
Samuel Chiomokhare ◽  
Ahmed M’Hamedi ◽  
Paul L Chazot
Keyword(s):  
Antioxidant Activity ◽  
Medicago Sativa ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Medicago Sativa L ◽  
Ethanol Extracts

Plant natural products have a long history of being a rich source of neuroactive drugs.The total phenolic and flavonoid content, and antioxidant activity of ethanol, chloroform and aqueous extracts of AlgerianMedicagoSativaL, leaves were measured to discover new potential sources of Algerian natural antioxidants and neuroactives. All extracts were non-neurotoxic up to 1 mg/ ml for 24 h exposure. The total phenolcontent of M.Sativa extracts ranged between 38 and 69 mg GAE per g extracts. All of the extracts displayed antioxidant capacityassessed by two methods, but at different levels depending on the concentration and the extraction solvent used. The overall antioxidant potency was in the order water > chloroform> ethanol extracts, and the phenolic content exhibited a positive correlation with the antioxidant activity of M. Sativa extracts.Despite a weaker antioxidant activity, the ethanol extract elicited a calcium spike and suppressed the KCl-induced depolarisation response in neurons, indicating effect on ligand-and/or voltage gated calcium channels. In contrast, the chloroform and water extractsdisplayed no apparent direct functional effects. In conclusion, the antioxidant and functional properties related to phenolic content of Algerian Medicago Sativa L. in water and ethanol extracts, respectively.

scholarly journals Antioxidant effect of Mn2+ on capacitation and acrosome reaction of fresh and chilled cattle bull semen

2012 ◽  
Vol 1 (1) ◽  
pp. 18
Author(s):  
Amrit Kaur Bansal ◽  
Ranjna Sundhey Cheema ◽  
Vinod Kumar Gandotra
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Acrosome Reaction ◽  
Reactive Oxygen ◽  
Antioxidant Effect ◽  
Oxygen Species ◽  
Sperm Capacitation ◽  
Bull Semen

The aim of this paper was to investigate the antioxidant effect of Mn2+ (200 mM) on the sperm capacitation and acrosome reaction of fresh and chilled cattle bull semen. It has been found that Mn2+ supplementation improves (P≤0.05) the motility at 0, 2, 4 and 6 h of incubation. MDA (malondialdehyde), end product of lipid peroxidation, decreases significantly (P≤0.05) with the supplementation of manganese at 0- and 6-hr of incubation both in fresh and chilled semen. Manganese also increases acrosome reaction significantly (P≤0.05) both in fresh and chilled semen at 0, 4 and 6 h of incubation. Therefore, our findings suggest the role of Mn2+supplementation in improving the quality of cattle bull semen by its scavenging property<em> i.e.</em> reduction in the production of reactive oxygen species during its storage at 4°C or incubation at 37°C for capacitation.

scholarly journals Protective effect of dehydroepiandrosterone against lipid peroxidation in a human liver cell line

1999 ◽  
pp. 35-39 ◽  
Author(s):  
M Gallo ◽  
M Aragno ◽  
V Gatto ◽  
E Tamagno ◽  
E Brignardello ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Protective Effect ◽  
Human Liver ◽  
Antioxidant Properties ◽  
Liver Cells ◽  
Threshold Concentration ◽  
Blue Staining ◽  
Lag Phase ◽  
Human Liver Cell ◽  
Chang Liver

OBJECTIVE: Dehydroepiandrosterone (DHEA) is a widely studied steroid hormone with multi-functional properties. Reports suggest that some of the many activities of DHEA are due to its protective effect against lipid peroxidation. Nevertheless, the antioxidant properties of DHEA are still the subject of debate. The aim was to evaluate whether its two opposed effects on lipid peroxidation reported in the literature may be dependent on schedule and doses used. METHODS: Chang liver cells, a line derived from normal human liver, were grown in media containing either no steroids (control) or DHEA at concentrations ranging from 0.1 micromol/l to 50 micromol/l. At specific times, cultures were halted and cells received a pro-oxidant stimulus (cumene (CuOOH) 0.5 mmol/l), at which time cell viability (by trypan blue staining and lactate dehydrogenase (LDH) release) and thiobarbituric acid reactive substances (TBARS) concentration (spectrophotometrical assay) were evaluated. RESULTS: At concentrations ranging from 0.1 micromol/l to 1 micromol/l, DHEA protects Chang liver cells against lipid peroxidation and/or death induced by cumene. This effect disappears if the concentration is increased to 10 micromol/l; at higher concentrations (50 micromol/l) a pro-oxidant/cytotoxic effect of DHEA appears. CONCLUSIONS: DHEA exhibits two opposed effects on lipid peroxidation; depending on its concentration it acts either to limit or to induce oxidative stress. The threshold concentration at which the pro-oxidant activity of DHEA prevails is not far in excess of that having an antioxidant effect. Either effect of DHEA on lipid peroxidation is only evident after a 'lag-phase'.

scholarly journals Antioxidant properties of resveratrol and piceid on lipid peroxidation in micelles and monolamellar liposomes

2008 ◽  
Vol 135 (1-3) ◽  
pp. 76-83 ◽  
Author(s):  
Sabrina Fabris ◽  
Federico Momo ◽  
Giampietro Ravagnan ◽  
Roberto Stevanato
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Properties
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