scholarly journals In Vitro Screening of Antioxidant Properties of Ten Cameroonian Medicinal Plants

2013 ◽  
Vol 3 (2) ◽  
pp. 171-182 ◽  
Author(s):  
Jules Clement Nguedia Assob ◽  
Abdel Jelil Njouendou ◽  
Pepin Efouet Alango Nkeng ◽  
Jean Rodolphe Chouna ◽  
S. M. Badami ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Total Phenolic ◽  
Methanolic Extracts ◽  
Nitric Oxide Radical ◽  
Phenolic Constituents

Background: Oxidative stress is involved in the pathogenesis of several degenerative diseases. This work studied the invitro anti-oxidative properties of methanolic extracts of 10 Cameroonian medicinal plants including Autranella congolensis(AC); Beilschmiedia anacardioides(BA); Crossopteryx febrifiga (CF); Cussonia arborea (Cu A); Cyphostema adenaucaule(Cy A); Dissotis longipetala (DL); Lonchocarpus sepium(LS); Nauclea pobeguinii (NP); Pycnanthus angolensis (PA);Picralima nitida (PN) used in folk medicine against various ailments associated with oxidative damages pattern. Method:The extracts were subjected to phytochemical screening to identify major classes of secondary metabolites. Their totalcontents in phenols, flavonoids were determined; whereas the anti-oxidative and reducing power were evaluated. Theseextracts were tested for their scavenging activity using DPPH, ABTS, superoxide, hydroxyl and nitric oxide radicals andhydrogen peroxide. The inhibitory effects of extracts against lipid peroxidation were also investigated on rat brainhomogenates. Results: The methanolic extracts of CF, DL, LS and AC showed important antioxidant activities andscavenging properties against all radicals system used. The extract of CuA and NP were inactive against hydrogen peroxide.The most potent antioxidant activity was obtained with AC extract, which showed the lowest IC50 value as compared to the standard. The IC50 values of this extract were 7.31±0.25, 5.20±0.35, 42.17±0.60, 77.30±7.41, 32.94±1.32, 9.71±1.07 and 121.59±1.08 μg/ml against ABTS, DPPH, hydrogen peroxide, inhibition of lipid peroxidation, superoxide radical, hydroxylradical and nitric oxide radical system respectively. These values were found to be comparable to those obtained with the standards. Significant positive correlations were observed between total antioxidant activity with total reducing power(p<0.01; R2=0.8190), total flavonoids (P<0.05; R2=0.6419) and total phenol (P<0.01; R2=0.8643); whereas non-significant correlation was obtained between other systems and the total phenolic constituents. Conclusion: The data generated from this study sustained the use of these medicinal plants in the treatment of various diseases associated with oxidative stress.The potent antioxidant activity of the Autranella congolensis extract and its high content in phenolic constituents are to betaken into the consideration for further phytochemical and biological investigations.

scholarly journals Variation in antioxidant activity at two ripening stages of wild mango, Spondias pinnata (L.f.) Kurz., an underutilized fruit

2020 ◽  
Vol 7 (4) ◽  
Author(s):  
Bince Mani ◽  
Sinjumol Thomas
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Total Antioxidant Activity ◽  
Plant Origin ◽  
Nitric Oxide Radical ◽  
Total Antioxidant

Antioxidant compounds play a significant role in preventing and scavenging free-radicals by reducing oxidative stress and providing protection to humans against degenerative diseases and infections. Obviously, antioxidant molecules of plant origin are pivotal to combat the oxidative harm in cells. Present work intended to evaluate the antioxidant capacity of the fruits of Spondias pinnata. Methanol extracts of the fruits at two stages of maturity were prepared and investigated by various antioxidants analyses such as total antioxidant activity, reducing power and radical scavenging assays (DPPH, nitric oxide and hydrogen peroxide). Its total phenol, flavonoid and tannin contents were also determined. Spondias pinnata fruit extracts exhibited effective antioxidant activity and its IC50 values of the unripe fruits were 65, 66, 72.23, 83.25, 66.75 ?g/ml and ripe fruits were 124.24, 92.50, 97.66, 144.10, 72.25 ?g/ml, for total antioxidant activity, reducing power, DPPH radical scavenging, nitric oxide radical scavenging and hydrogen peroxide scavenging assays respectively. The extracts, especially unripe fruit extract had good amounts of total phenolic and flavonoid contents which might contribute the antioxidant activities considerably. It is evident from the study that the fruits of S. pinnata possessed potent antioxidant activity and it can be considered as a good dietary choice among the underutilized fruits as well as common fruits. Therefore, wild mango may be a good choice of antioxidants of plant origin for dietary and pharmaceutical uses.

scholarly journals Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

2017 ◽  
Vol 71 (5) ◽  
pp. 361-370
Author(s):  
Slavica Grujic ◽  
Ana Dzamic ◽  
Violeta Mitic ◽  
Vesna Stankov-Jovanovic ◽  
P.D. Marin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Scavenging Activity

Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS, FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin?Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloroform (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 ?mol Fe/mg. The best radical scavenger was methanol (1.04 ?mol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxidant activity. The best reducing capacity was obtained with methanol extract of L. purpureum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties.

scholarly journals Antioxidant Activities of the Aqueous Extracts of Pedalium murex D. Royen EX L. Fruit and Leafy Stem

2021 ◽  
pp. 1-9
Author(s):  
Gerard Bessan Dossou-Agoin ◽  
Habib Ganfon ◽  
Fidèle Assogba ◽  
Adam Gbankoto ◽  
Joachim Gbenou ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Aqueous Extracts ◽  
Fruit Extract ◽  
Stem Extract ◽  
Dpph Scavenging

Aims: The leafy stem and fruit of P.murex have been reported to be used in folk medicine to treat male reproductive system ailments. This study was undertaken to assess the antioxidant potential of the aqueous extracts of P.murex leafy stem and fruit. Methodology: Extracts were prepared by macerating the powder in water. Total phenolics amount were determined by Folin-Ciocalteu reagent, flavonoids were quantified by aluminum chloride method and total tannin content was estimated by hexacyanoferric method. The in vitro antioxidant activity of the extracts were assessed through 2,2´-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, reducing power assay, hydrogen peroxide (H2O2) inhibition assay and lipid peroxidation assay. Results: Total phenolic compounds, flavonoids and tannins content were respectively equal to 48.91± 1.67 µg/mg Gallic Acid Equivalents (GAE); 56.01± 0.90 µg/mg Rutin Equivalents (RUE); 16.92± 1.22 µg/mg Tannic Acid Equivalents (TAE) for the leafy stem extract while they were equal to 26.26± 0.54 µg/mg GAE; 47.88± 2.39 µg/mg RUE; 7.94± 0.31 µg/mg TAE for the fruit. The leafy stem extract exhibited a more pronounced DPPH scavenging activity, reducing power, hydrogen peroxide scavenging activity and lipid peroxidation activity than the fruit extract. Conclusion: The antioxidant activity of the leafy stem aqueous extract was significantly more potent than that of the fruit extract. Further studies will find out the extracts pharmacological efficacy and innocuity.

scholarly journals Antioxidant Activity and Total Phenolic Content in Shiitake Mycelial Exudates

2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Weihuan Huang ◽  
Joo-Shin Kim ◽  
Hau Yin Chung
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Shiitake Mushroom ◽  
Ferrous Ions

Exudates (DE) secreted from two shiitake mushroom mycelia (strains 1358 and L5458) were evaluated for their antioxidative properties and phenolic content. 1358DE and L5458DE showed distinct antioxidant activity in different in vitro assays, including scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, hydroxyl radical, superoxide anions and hydrogen peroxide; the ability to chelate ferrous ions; reducing power; hemolysis inhibition activity in rat erythrocyte; and lipid peroxidation inhibition (IC50 values of 1358DE and L5458DE were 3.3 and 132.6; 44.5 and > 1000; 26.9 and 53.7; 153.6 and >175.0; 176.0 and 521.0; 26.7 and 746.4; 47.8 and 736.9; and 3.1 and > 1000 μg/mL, respectively). Their total phenolic content was 237.33 and 24.08 mg gallic acid equivalent (GAE)/g of dry DE, respectively. Overall, these results show that 1358DE generally possesses better antioxidant properties than L5458DE, possibly due to its larger total phenolic content. Shiitake mushroom mycelial exudates, particularly of 1358DE, could be a good source of natural antioxidants.

scholarly journals Antioxidant properties of Ageratum conyzoides L. Asteraceae leaves

2018 ◽  
Vol 53 (4) ◽  
pp. 265-276 ◽  
Author(s):  
FO Adetuyi ◽  
KO Karigidi ◽  
ES Akintimehin ◽  
ON Adeyemo
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Erectile Dysfunction ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Tissue Homogenate ◽  
Total Phenolic ◽  
Ageratum Conyzoides

In vitro antioxidative and anti-lipid peroxidative properties of aqueous and methanol extracts of Ageratum conyzoides leaves were studied in controlling erectile dysfunction caused by oxidative stress. Methanol extract gave a significantly (P ˂ 0.05) higher content of total phenolic (61.4 mgGAE/g), total flavonoid (42.2 mgQE/g), ascorbic (10.1 mgAAE/100g) and phosphomolybdate (45.8 mgAAE/g) than the aqueous extract. The result showed that the extracts have high antioxidant activities. However, the methanol extract showed a higher DPPH and hydrogen peroxide scavenging activities over aqueous extract but the aqueous extract had a higher reducing power. The methanol extract exhibited a greater inhibition against lipid peroxidation induced by Fe2+ in rat pancreas and penile tissue homogenate exemplified by their least IC50 (94.21 μg/ml in pancreas) and (75.95 μg/mL in penile tissue) while in rat brain homogenate the aqueous extract exhibited a greater inhibition against lipid peroxidation induced by Fe2+ with least IC50 of 91.74 μg/mL. Hence, these extracts can be used as a potent natural antioxidant against free radicals and as a natural source of combating erectile dysfunction caused by oxidative stress. The extracts of Ageratum conyzoides leaves could be useful therapeutically as erectogenic agent.Bangladesh J. Sci. Ind. Res.53(4), 265-276, 2018

Antioxidant Properties of Ethanolic Extract from Ramulus mori (Sangzhi)

2009 ◽  
Vol 15 (5) ◽  
pp. 435-444 ◽  
Author(s):  
Zuofa Zhang ◽  
Jie Jin ◽  
Liangen Shi
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Radical ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Experimental Models ◽  
Hplc Method ◽  
Total Phenolic ◽  
Dependent Manner

The antioxidant properties and total phenolic contents of four fractions of ethanolic extract from Ramulus mori were examined. Various experimental models including superoxide radical, hydroxyl radical, 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH) scavenging activity, metal chelating activity, and reducing power were used for characterization of their antioxidant activity. The four fractions showed various degrees of efficacy in each assay in a dose-dependent manner. The third fraction with the highest amount of total phenolics was the most potent antioxidant in all assays used. In addition, the most powerful compound (oxyresveratrol) was isolated and identified followed by on-line HPLC method and characterized by different spectral analysis. Oxyresveratrol exhibited impressive antioxidant activities in scavenging the superoxide radical, hydroxide radical, and DPPH. On the basis of the results obtained, Ramulus mori may serve as a potential source of natural antioxidant due to its significant antioxidant activity and oxyresveratrol may be the most powerful antioxidant in ethanolic extracts of Ramulus mori.

scholarly journals Free-radical Scavenging and Quantitative Estimation of Flavonoids from the Polar Extracts of Corchorus depressus Leaves

2021 ◽  
pp. 210-219
Author(s):  
Khuntia Tapas Kumar ◽  
Nanda Upendra Nath ◽  
Senapati Aswini Kumar
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Nitric Oxide Radical ◽  
Polar Extracts

Background: The investigation of total flavonoids and antioxidant activity of polar extracts of Corchorus depressus is the major aim of this study. As observed from ancient literatures and folkloric claims the plant Corchorus depressus worshipped by the married women of Odisha, India, in the rituals called as “Jama Jutia”, possesses different biological activities including antioxidant property. Methods: The diphenyl picryl hydrazine, hydroxyl radical and nitric oxide radical scavenging methods were performed for measurement of the antioxidant activity at different extracts. The flavonoid and phenolic content of the extracts were determined by using aluminium chloride and Folin-Ciocalteau’s reagent (FCR) methods respectively. Results: The results for estimation of total phenolic content (mg/ 100 g) expressed as gallic acid equivalent (GAE) and total flavonoid (mg/ 100 g) in weight of quercetin equivalent (QE) was highest in methanolic extract 78.46 and  21.2 respectively, followed by 18.18 mg/100 g in GAE and 1.80 mg/100 g in QE for aqueous extract. Conclusion: The methanolic extract of C. depressus at 100µg/ml showed highest DPPH, hydroxyl and nitric oxide radical scavenging activity and this activity may be attributed to the presence of saponins and flavonoids as detected in the extract.

Antioxidant and antifungal activities of Myrianthus arboreus P. Beauv. (Moraceae), Allanblackia gabonensis Pellegr. (Clusiaceae) and three other Cameroonian medicinal plants

2021 ◽  
Vol 4 (1) ◽  
pp. 1-7
Author(s):  
Aimé G. Fankam ◽  
Serge S. Atsafack ◽  
Guy S. S. Njateng ◽  
Jules R. Kuiate
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Medicinal Plants ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Bark Extract ◽  
Total Phenolic ◽  
Antifungal Activities ◽  
Allanblackia Gabonensis

Background: Biological activities of medicinal plants make them attractive alternative complementary medicines. Therefore, this study aimed to assess the antioxidant and antifungal activities of the methanol extract of Myrianthus arboreus, Allanblackia gabonensis, Gladiolus quartinianus, Peperomia fernandopoiana and Vepris soyauxii. Methods: Total phenolic contents (TPC) and flavonoid contents (FC) were evaluated using standard methods. The antioxidant activity of the extracts was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing power assays. Antifungal activity was evaluated by the broth micro-dilution method. Results: TPC of extracts ranging from 67.89 to 196.01 mgGAE/g TPC were very higher in P. fernandopoiana whole plant (PFW) extract (196.01±10.25 mgGAE/g) and MAL (165.71±10.68 mgGAE/g) whereas the A. gabonensis fruit extract (AGF) and M. arboreus bark extract (MAB) had the highest flavonoid content (66.54 ± 2.12 and 48.13 ± 1.65 mgEC/g of extract, respectively). Apart from M. arboreus leave extract (MAL), all extracts showed important DPPH radical scavenging activity (IC50 < 20 μg/mL). AGFl and PFW exhibited significant ferric reducing power compared to ascorbic acid and other extracts (P<0.05). Each of the tested extracts showed antifungal activity against atleast one of the five pathogenic fungi, with MICs ranging from 128 to 1024 µg/mL. MAL was active on all the tested fungi whereas MAB showed the highest antifungal activity (MIC=128 µg/mL). Conclusion: The findings of this study emphasize the evidence that the methanol extracts of the leave and bark of Myrianthus arboreus could be an alternative to fight against oxidative stresses and infections due to opportunistic yeast pathogens. Keywords: Oxidative stress; fungal infection; medicinal plant; antioxidant activity; antifungal activity.

scholarly journals Antioxidant Capacity and Radical Scavenging Effect of Polyphenol RichMallotus philippenensisFruit Extract on Human Erythrocytes: AnIn VitroStudy

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Mayank Gangwar ◽  
Manish Kumar Gautam ◽  
Amit Kumar Sharma ◽  
Yamini B. Tripathi ◽  
R. K. Goel ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Hplc Analysis ◽  
Radical Scavenging ◽  
Human Erythrocytes ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Scavenging Effect ◽  
Radical Scavenging Effect

Mallotus philippinensisis an important source of molecules with strong antioxidant activity widely used medicinal plant. Previous studies have highlighted their anticestodal, antibacterial, wound healing activities, and so forth. So, present investigation was designed to evaluate the total antioxidant activity and radical scavenging effect of 50% ethanol fruit glandular hair extract (MPE) and its role on Human Erythrocytes. MPE was tested for phytochemical test followed by its HPLC analysis. Standard antioxidant assays like DPPH, ABTS, hydroxyl, superoxide radical, nitric oxide, and lipid peroxidation assay were determined along with total phenolic and flavonoids content. Results showed that MPE contains the presence of various phytochemicals, with high total phenolic and flavonoid content. HPLC analysis showed the presence of rottlerin, a polyphenolic compound in a very rich quantity. MPE exhibits significant strong scavenging activity on DPPH and ABTS assay. Reducing power showed dose dependent increase in concentration absorption compared to standard, Quercetin. Superoxide, hydroxyl radical, lipid peroxidation, nitric oxide assay showed a comparable scavenging activity compared to its standard. Our finding further provides evidence thatMallotusfruit extract is a potential natural source of antioxidants which have a protective role on human Erythrocytes exhibiting minimum hemolytic activity and this justified its uses in folklore medicines.

scholarly journals Inhibition of Fe2+ Induced Lipid Peroxidation in Rats Brain by Extract of Euphorbia heterophylla in vitro

2019 ◽  
pp. 1-8
Author(s):  
Leye Jonathan Babatola ◽  
Oluwakemisola B. Oshanimi ◽  
Olanrewaju M. Oluba ◽  
Lawrence Okoror ◽  
Adewale Agboola Odutuga
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenol ◽  
Dependent Manner ◽  
Significant Difference

This study is sought to determine the antioxidant activity and protective ability of aqueous and methanol extractible phytochemicals from Euphorbia heterophylla leaves on lipid peroxidation induced in rat brain by pro-oxidant, in vitro. The extracts of the leaves were prepared, and the ability of the extracts is to inhibit 25 µM FeSO4 induced lipid peroxidation in isolated rats’ brain, were determined. Thereafter, total phenol content, reducing power (FRAP), Fe (II) chelating, and DPPH* free radical scavenging ability of the extracts was determined and considered as an index of antioxidant activity. The results revealed that the extracts inhibit malondialdehyde (MDA) production in the basal and pro-oxidant induced lipid peroxidised rats in a dose-dependent manner, [methanol 80.11%, aqueous 70.3%] with the methanol extract (MEE) significantly (P< 0.05) than that of aqueous extract (AEE). The methanol extract (0.74 ± 0.6 mg/g) had higher total phenol contents than the aqueous (0.57 ± 1.2 mg/g); likewise the methanol extract had higher reducing power (0.08 ± 0.2, 0.03 ± 0.1 mg/g), but had no significant difference in Fe (II) chelating ability (EC50= 0.34, 0.36) with DPPH* scavenging ability (EC50=0.075, 0.075). This antioxidant properties and the protective effect of this leaf could be harnessed in the management and prevention of degenerative diseases in association with oxidative stress.

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