scholarly journals Validation of Pedigree Relationships Using a Multiplex Microsatellite Marker Assay in Iranian Holstein Cattle / Walidacja rodowodów irańskiego bydła holsztyńskiego przy użyciu analizy multipleks markerów mikrosatelitarnych

2013 ◽  
Vol 13 (3) ◽  
pp. 481-493
Author(s):  
Mona Hashemi ◽  
Cyrus Amirinia ◽  
Mohammad Taher Harkinezhad ◽  
Mohammad Hossein Banabazi ◽  
Arash Javanmard ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
High Efficiency ◽  
Shannon Index ◽  
Holstein Cattle ◽  
Pedigree Structure ◽  
Highly Sensitive ◽  
Identity Test ◽  
Identification Analysis ◽  
Marker Assay ◽  
Parentage Control

Abstract In the present study, the pedigree and genotype data of 94 individuals were examined for accuracy of parentage allocations and identity test using 12 fluorescent-labelled microsatellite markers in a highly sensitive and accurate ABI system. The panel of 12 markers showed mean PIC value of 0.71 and Shannon index of 1.65 and 7.58 alleles per locus, which suggests that these markers are highly polymorphic and could be useful for parentage control. Based on scoring allele sizes, a total of 91 alleles were observed within the studied population. The highest and lowest number of alleles was observed for TGLA227 locus and TGLA126 and BM1818 loci, respectively. The pedigree was considered incorrect in seven (35%) out of all the evaluated progeny, as their genotype did not match their parents. Combined EP value obtained for all loci in both parentage and identification analysis was 0.99, which indicates the high efficiency of the studied marker set and the accuracy of genotyping in ABI systems. Finally, the present findings confirmed the importance of surveying the pedigree structure and efficiency of 12 fluorescent-labelled microsatellite markers in a single multiplex PCR for parentage testing in the sampled Holstein cattle population.

Core/shell Ag@silicate nanoplatelets and poly(vinyl alcohol) spherical nanohybrids fabricated by coaxial electrospraying as highly sensitive SERS substrates

RSC Advances ◽  
2016 ◽  
Vol 6 (71) ◽  
pp. 67204-67211 ◽  
Author(s):  
Chih-Wei Chiu ◽  
Po-Hsien Lin
Keyword(s):  
Vinyl Alcohol ◽  
Large Scale ◽  
High Efficiency ◽  
Poly Vinyl Alcohol ◽  
Core Shell ◽  
Sers Substrate ◽  
Sers Substrates ◽  
Highly Sensitive ◽  
Coaxial Electrospray

A novel flexible, freestanding, large-scale, and disposable SERS substrate of core/shell Ag@silicate and poly(vinyl alcohol) spherical nanohybrids, fabricated by coaxial electrospray, allows for the high-efficiency detection of adenine from DNA.

scholarly journals Random terpolymer based on thiophene-thiazolothiazole unit enabling efficient non-fullerene organic solar cells

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jingnan Wu ◽  
Guangwei Li ◽  
Jin Fang ◽  
Xia Guo ◽  
Lei Zhu ◽  
...  
Keyword(s):  
Solar Cells ◽  
Organic Solar Cells ◽  
High Performance ◽  
High Efficiency ◽  
Temperature Dependent ◽  
Polymer Backbone ◽  
Positive Effects ◽  
Highly Sensitive ◽  
Aggregation Property ◽  
Reduced Temperature

Abstract Developing a high-performance donor polymer is critical for achieving efficient non-fullerene organic solar cells (OSCs). Currently, most high-efficiency OSCs are based on a donor polymer named PM6, unfortunately, whose performance is highly sensitive to its molecular weight and thus has significant batch-to-batch variations. Here we report a donor polymer (named PM1) based on a random ternary polymerization strategy that enables highly efficient non-fullerene OSCs with efficiencies reaching 17.6%. Importantly, the PM1 polymer exhibits excellent batch-to-batch reproducibility. By including 20% of a weak electron-withdrawing thiophene-thiazolothiazole (TTz) into the PM6 polymer backbone, the resulting polymer (PM1) can maintain the positive effects (such as downshifted energy level and reduced miscibility) while minimize the negative ones (including reduced temperature-dependent aggregation property). With higher performance and greater synthesis reproducibility, the PM1 polymer has the promise to become the work-horse material for the non-fullerene OSC community.

scholarly journals Using microsatellite markers to analyze genetic diversity in 14 sheep types in Iran

2017 ◽  
Vol 60 (3) ◽  
pp. 183-189 ◽  
Author(s):  
Mohammad Taghi Vajed Ebrahimi ◽  
Mohammadreza Mohammadabadi ◽  
Ali Esmailizadeh
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Information Content ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
Extraction Procedure ◽  
Shannon Index ◽  
Group Method ◽  
Salting Out ◽  
Pair Group

Abstract. Investigation of genetic relationship among populations has been traditionally based on the analysis of allele frequencies at different loci. The prime objective of this research was to measure the genetic polymorphism of five microsatellite markers (McMA2, BM6444, McMA26, HSC, and OarHH35) and study genetic diversity of 14 sheep types in Iran. Genomic DNA was extracted from blood samples of 565 individuals using an optimized salting-out DNA extraction procedure. The polymerase chain reaction (PCR) was successfully performed with the specific primers. Some locus–population combinations were not at Hardy–Weinberg equilibrium (P < 0. 05). The microsatellite analysis revealed high allelic and gene diversity in all 14 breeds. Pakistani and Arabi breeds showed the highest mean number of alleles (11.8 and 11 respectively), while the highest value for polymorphic information content was observed for the Arabi breed (0.88). A UPGMA (unweighted pair group method with arithmetic mean) dendrogram based on the Nei's standard genetic distance among studied breeds showed a separate cluster for Arabi and Pakistani breeds and another cluster for other breeds. The Shannon index (H0) for McMA2, BM6444, McMA26, HSC, and OarHH35 was 2.31, 2.17, 2.27, 2.04 and 2.18, respectively, and polymorphic information content (PIC) values were 0.88, 0.92, 0.87, 0.84, and 0.86 for McMA2, BM6444, McMA26, HSC, and OarHH35, respectively. The high degree of variability demonstrated within the studied sheep types implies that these populations are rich reservoirs of genetic diversity that must be preserved.

Hydrothermal synthesis of WO3 nanoplates as highly sensitive cyclohexene sensor and high-efficiency MB photocatalyst

2013 ◽  
Vol 181 ◽  
pp. 537-543 ◽  
Author(s):  
Xiaoqing Gao ◽  
Xintai Su ◽  
Chao Yang ◽  
Feng Xiao ◽  
Jide Wang ◽  
...  
Keyword(s):  
Hydrothermal Synthesis ◽  
High Efficiency ◽  
Highly Sensitive

Synthesis of TiO2–WO3 nanocomposites as highly sensitive benzene sensors and high efficiency adsorbents

2012 ◽  
Vol 22 (28) ◽  
pp. 13914 ◽  
Author(s):  
Ying Zhu ◽  
Xintai Su ◽  
Chao Yang ◽  
Xiaoqing Gao ◽  
Feng Xiao ◽  
...  
Keyword(s):  
High Efficiency ◽  
Highly Sensitive

scholarly journals A yeast BiFC-seq method for genome-wide interactome mapping

2020 ◽  
Author(s):  
Limin Shang ◽  
Yuehui Zhang ◽  
Yuchen Liu ◽  
Chaozhi Jin ◽  
Yanzhi Yuan ◽  
...  
Keyword(s):  
Ebola Virus ◽  
High Efficiency ◽  
Bimolecular Fluorescence Complementation ◽  
Economical Method ◽  
Protein Protein Interaction ◽  
Genome Wide ◽  
Highly Sensitive ◽  
Tumor Protein P53 ◽  
Interactome Mapping ◽  
Novel Interactions

AbstractGenome-wide physical protein-protein interaction (PPI) mapping remains a major challenge for current technologies. Here, we report a high-efficiency yeast bimolecular fluorescence complementation method coupled with next-generation DNA sequencing (BiFC-seq) for interactome mapping. We applied this technology to systematically investigate an intraviral network of Ebola virus (EBOV). Two-thirds (9/13) of known interactions of EBOV were recaptured and five novel interactions were discovered. Next, we used BiFC-seq method to map the interactome of the tumor protein p53. We identified 97 interactors of p53 with more than three quarters are novel. Furthermore, in more complex background, we screened potential interactors by pooling two BiFC-libraries together, and revealing a network of 229 interactions among 205 proteins. These results show that BiFC-seq is a highly sensitive, rapid and economical method in genome-wide interactome mapping.

scholarly journals Evaluation of genetic variability among “Early Mature”Juglans regiausing microsatellite markers and morphological traits

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3834 ◽  
Author(s):  
Aziz Ebrahimi ◽  
Abdolkarim Zarei ◽  
Mojtaba Zamani Fardadonbeh ◽  
Shaneka Lawson
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Normal Growth ◽  
Diversity Indices ◽  
Shannon Index ◽  
Phenotypic Trait ◽  
Phenotypic Traits ◽  
High Genetic Diversity ◽  
Early Maturation ◽  
Persian Walnut

Limiting the juvenile phase and reducing tree size are the two main challenges for breeders to improve most fruit crops. Early maturation and dwarf cultivars have been reported for many fruit species. “Early mature” and low vigor walnut genotypes were found among seedlings of Persian walnut. Nine microsatellite markers were used to evaluate genetic diversity among “Early Mature” Persian walnut accessions and provide a comparison with “normal growth” accessions. Six maturation related characteristics were also measured in “Early Mature” samples. Phenotypic traits and diversity indices showed relatively high levels of genetic diversity in “Early Mature” seedlings and indicated high differentiation between individuals. Seedling height, the most diverse phenotypic trait, has an important role in the clustering of “Early Mature” accessions. The “Early Mature” type had higher number of alleles, number of effective allele, and Shannon index compared to the “Normal Growth” group. The two types of studied walnuts had different alleles, with more than half of produced alleles specific to a specific group. “Early Mature” and “Normal Growth” walnuts had 27 and 17 private alleles, respectively. Grouping with different methods separated “Early Mature” and “Normal Growth” samples entirely. The presence of moderate to high genetic diversity in “Early Mature” walnuts and high genetic differentiation with “Normal Growth” walnuts, indicated that “Early Mature” walnuts were more diverse and distinct from “Normal Growth” samples. Moreover, our results showed SSR markers were useful for differentiating between “Early Mature” and “Normal Growth” walnuts. A number of identified loci have potential in breeding programs for identification of “Early Mature” walnuts at the germination phase.

scholarly journals Transferability and Characterization of Microsatellite Markers from Byrsonima Cydoniifolia A. Juss. (MALPIGHIACEAE) in Seven Related Taxa from Cerrado Biome Reveal Genetic Relationships

2021 ◽  
Author(s):  
Vanessa Bernardes ◽  
Devanir M. Murakami ◽  
Nair Bizão ◽  
Tamara N. Souza ◽  
Marcos J. da Silva ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Genetic Divergence ◽  
High Efficiency ◽  
Population Studies ◽  
Genetic Relationships ◽  
Species Occurrence ◽  
Genetic Population ◽  
Expected Heterozygosity

Abstract Byrsonima Rich. is one of the largest genera of the Malpighiaceae family, with 97 species occurrence in Brazil. In this study, 17 microsatellite markers previously developed and characterized in Byrsonima cydoniifolia A. Juss. were tested for seven related taxa. All species tested here are native to Brazil, and of these species four are endemic. Cross-amplification as successfully optimized with high efficiency for all species. Microsatellite markers panels ranged from 11 (64,8%) transfered markers in B. viminifolia to 6 (35.2%) in B. umbellata. All loci were genotyped for 16 individuals of each species, except for B. viminifolia (14 individuals), then the polymorphic loci were characterized. The total number of alleles per locus across tested species ranged from 5 (B. linearifolia) to 8 (B. subterranea) alleles. B. umbellata showed lower values of observed and expected heterozygosity (Ho = 0.312; He = 0.436) and B. subterranea presented the highest values (Ho = 0.687; He = 0.778). A greater number of microsatellite markers should be developed for B. umbellata, once that the markers set transferred is reasonably informative, due to the greatest genetic divergence between species. The microsatellite marker panels transferred to the species B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. viminifolia and B. linearifolia are very informative, with a high combined probability of exclusion of paternity (Q ≥ 0.976) and the low combined probability of identity (I ≤ 9.91x10− 6), potentially suitable for future genetic-population studies, supporting strategies for maintaining the genetic diversity and for exploration of Byrsonima species as genetic resources.

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