scholarly journals Potential of graded doses of neem (Azadirachta indica) seed oil on ruminal fermentation characteristics, degradability, and methane formation in vitro

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Waleed Al-Marzooqi ◽  
Sobhy M. Sallam ◽  
Othman Alqaisi ◽  
Hani M. El-Zaiat
Keyword(s):  
Azadirachta Indica ◽  
Seed Oil ◽  
Basal Diet ◽  
Linear Increase ◽  
Methane Formation ◽  
Ruminal Fermentation ◽  
Long Term Conditions ◽  
Tropical Regions ◽  
Neem Seed Oil

Abstract Neem (Azadirachta indica) belongs to Meliaceae family, represented mainly by trees, and widely cultivated and adapted in many tropical regions. The objective of this study was to evaluate the effect of increasing doses of neem seed oil (NSO) on ruminal methane (CH4) formation, diet degradability, and fermentation characteristics 24 h of in vitro incubation. Treatments were randomly designed to four doses of NSO supplemented to the basal diet (0, 20, 40, or 60 ml/kg DM). Increasing NSO dose resulted in a quadratic decrease (P < 0.05) in net gas (expressed as ml/g DM and ml/g TDOM) and CH4 (expressed as ml/g TDNDF) production, while CH4 (expressed as ml/g TDOM), acetate and propionate proportions decreased linearly confirming a dose-related effect. A quadratic increase in TDOM and linear increase (P = 0.023) in DNDF, NH3-N concentrations, and total protozoal counts were observed. However, a linear increase (P = 0.009) was found in the ruminal butyrate proportion and partitioning factor as dietary NSO supplementation increased. In conclusion, dietary NSO supplementation mediated some desirable fermentation patterns, reducing ruminal NH3-N concentration and CH4 production with some adverse effects on fiber degradability. However, practical research under long-term conditions is required for further investigation.

scholarly journals In vitro bioactivity of various pure flavonoids in ruminal fermentation, with special reference to methane formation

2018 ◽  
Vol 63 (No. 8) ◽  
pp. 293-304 ◽  
Author(s):  
Sinz Susanne ◽  
Kunz Carmen ◽  
Liesegang Annette ◽  
Braun Ueli ◽  
Marquardt Svenja ◽  
...  
Keyword(s):  
Tannic Acid ◽  
Epigallocatechin Gallate ◽  
Basal Diet ◽  
Ammonia Concentration ◽  
Positive Control ◽  
Negative Control ◽  
Test Method ◽  
Methane Formation ◽  
Ruminal Fermentation

Polyphenols, like flavonoids, have been investigated when present in intact plants or in extracts as methane mitigating dietary supplements in ruminants. The aim of the present study was to examine pure compounds in a short-term in vitro experiment using the Hohenheim Gas Test method. We focused on the group of the flavonoids and tested which of them had the potential to mitigate methane without negatively affecting ruminal fermentation. Eight flavonoids were tested: epicatechin, luteolin-7-glucoside, quercetin, and isoquercetin in Experiment 1; catechin, gallocatechin, epigallocatechin, and epigallocatechin gallate in Experiment 2. Tannic acid, no flavonoid but a phenolic acid with known methane mitigating properties, served as positive control, and the unsupplemented basal diet as negative control. In both experiments, each of these compounds (including tannic acid) was tested at dosages of 0.5, 5.0, and 50.0 mg/g basal diet dry matter (DM) in four runs each. Gallocatechin, tannic acid, and epigallocatechin gallate (50 mg/g DM) lowered fermentation gas formation and in vitro organic matter digestibility relative to the negative control (Experiment 2). Apart from tannic acid, epicatechin, quercetin, isoquercetin, and luteolin-7-glucoside (5 and 50 mg/g DM) reduced the amount of CH<sub>4</sub> produced in relation to total gas produced (Experiment 1). The incubation fluid ammonia concentration was decreased with luteolin-7-glucoside and tannic acid (50 mg/g DM). From the flavonoids tested especially luteolin-7-glucoside seems to have a similar potential as tannic acid to mitigate methane and ammonia formation during ruminal fermentation in vitro, both favourable in environmental respect. These results need to be confirmed in live animals.

scholarly journals Chemical Composition and In Vitro Evaluation of the Mosquito (Anopheles) Repellent Property of Neem (Azadirachta indica) Seed Oil

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Olive Aidoo ◽  
Noble Kuntworbe ◽  
Fredrick William Akuffo Owusu ◽  
Deryl Nii Okantey Kuevi
Keyword(s):  
Azadirachta Indica ◽  
Seed Oil ◽  
Skin Irritation ◽  
Time Frame ◽  
Flow Properties ◽  
In Vitro Evaluation ◽  
Evaluation Approach ◽  
Neem Seed Oil ◽  
Mosquito Repellents

As one of the killer diseases in the world, malaria contributes to child mortality and global death annually. As a result, many reactive mechanisms have evolved to control and repel mosquitoes. The use of synthetic mosquito repellents with N,N-Diethyl-meta-toluamide (DEET) is one of the popular interventions despite its dermatological limitations such as skin irritations. Ethnobotanical reviews have identified that the adoption of natural repellents promises high repellence on mosquitoes with minimal side effects compared with synthetic ones. However, this has received little attention in modern pharmaceutical literature. This research is focused on the formulation of a natural mosquito repellent from the oil extracted from Azadirachta indica (A. Juss). The oil cream was formulated in concentrations of 10% v / w , 12.5% v / w , 15% v / w , 17.5% v / w , and 20% v / w using an in vitro evaluation approach. Pharmacopoeia characteristics of the cream such as pH, viscosity, spreadability, and organoleptic properties were carried out to verify acidity, permeation, and flow properties of the formulated cream. The spreadability rate was inversely proportional to the concentration of the cream in terms of oil content falling from 1.24 gm/s to 0.84 gm/s from concentrations 10% v / w to 20% v / w correspondingly. Skin irritation tests, however, indicated traces of irritation at 20% v / w . Repellency properties of the cream revealed a lasting effect on the mosquitoes, although this was dependent on concentration levels. Formulations of 17.5% v / w and 20% v / w neem seed oil cream had an equal repellency effect of 87.5%, whereas the synthetic repellent had a repellency of 75% within a justifiable time frame for all the formulations. This work shows that plant-based mosquito repellents promise a healthier approach in controlling mosquito bites, protecting the skin, and preventing malaria.

scholarly journals Pomegranate seed oil rich in conjugated linolenic acids reduces in vitro methane production

1970 ◽  
Vol 46 (3) ◽  
pp. 325-335
Author(s):  
E. Maleki ◽  
G.Y. Meng ◽  
M. Faseleh Jahromi ◽  
R. Jorfi ◽  
A. Khoddami ◽  
...  
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Seed Oil ◽  
Control Diet ◽  
Nutrient Utilization ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Ruminal Fermentation ◽  
Ch4 Production

The objective of this study was to determine the effect of pomegranate (Punica granatum L.) seed oil (PSO) on gas and methane (CH4) production, ruminal fermentation and microbial populations under in vitro conditions. Three treatments consisting of a control diet containing 10 mg tallow (CON); the control diet with 5 mg PSO + 5 mg tallow (MPSO) and the control diet containing 10 mg PSO (HPSO) were compared. Ten mg of the experimental fat/oil samples were inserted into a gas-tight 100 mL plastic syringe containing 30 mL of an incubation inoculum and 250 mg of a basic substrate of a hay/concentrate (1/1, w/w) mixture. In vitro gas production was recorded over 0, 2, 4, 6, 8, 10, 12 and 24 h of incubation. After 24 hours, incubation was stopped, and methane production, pH, volatile fatty acids (VFAs) and microbial counts were measured in the inoculant. Gas production at 4, 6, 8, 10, 12 and 24 h incubation, metabolizable energy and in vitro organic matter disappearance increased linearly and quadratically as level of PSO increased. Furthermore, the 10 mg PSO (HPSO) decreased CH4 production by 21.0% compared with the control (CON) group. There were no significant differences in total and individual VFA concentrations between different levels of PSO, except for butyric acid. After 24 h of incubation, methanogenesis decreased in the HPSO compared with the MPSO and CON treatments. In addition, total bacteria and protozoa counts increased with rising PSO levels, while population methanogenesis declined significantly. These results suggested that PSO could reduce methane emissions, which might be beneficial to nutrient utilization and growth in ruminants.

Study of anti-inflammatory effect of neem seed oil (Azadirachta indica) on infected albino rats

2014 ◽  
Vol 1 (3) ◽  
pp. 66 ◽  
Author(s):  
ManasRanjan Naik ◽  
Divya Agrawal ◽  
Rasmirekha Behera ◽  
Ayon Bhattacharya ◽  
Suhasini Dehury ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Seed Oil ◽  
Albino Rats ◽  
Neem Seed ◽  
Neem Seed Oil ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Effects of black seed oil and Ferula elaeochytris supplementation on ruminal fermentation as tested in vitro with the rumen simulation technique (Rusitec)

2015 ◽  
Vol 55 (6) ◽  
pp. 736 ◽  
Author(s):  
F. Klevenhusen ◽  
K. Deckardt ◽  
Ö. Sizmaz ◽  
S. Wimmer ◽  
A. Muro-Reyes ◽  
...  
Keyword(s):  
Volatile Fatty Acids ◽  
Seed Oil ◽  
Nigella Sativa ◽  
Feed Additives ◽  
Simulation Technique ◽  
Ruminal Fermentation ◽  
Bioactive Components ◽  
Black Seed

Plant bioactive compounds are currently viewed as possible feed additives in terms of methane mitigation and improvement of ruminal fermentation. A range of analyses, including the botanical characterisation, chemical composition and in vitro efficiency, have to be conducted before testing the compounds in vivo. Therefore, the aims of this study were (1) to identify the main bioactive components of black seed (Nigella sativa) oil (BO) and of the root powder of Ferula elaeochytris (FE), and (2) to investigate their effects on ruminal fermentation in vitro, when supplemented in different dosages to a diet (1 : 1, forage : concentrate), using the rumen simulation technique (Rusitec). Main compounds of BO were thymoquinone and p-cymene and α-pinene in FE. Supplementation of the diet with BO and FE did not affect concentration of volatile fatty acids but ammonia concentrations decreased with both supplements (P < 0.001). No effects of supplements on protozoal counts were detected but in vitro disappearance of DM and organic matter tended to increase with 50 mg/L FE (P < 0.1), compared with the control.

scholarly journals In vitro Effect of Essential Oils on Rumen Fermentation and Microbial Nitrogen Yield of High Concentrate Dairy Cow Diet

2016 ◽  
Vol 16 (2) ◽  
pp. 333-341
Author(s):  
Seyed Masoud Davoodi ◽  
Mohsen Danesh Mesgaran ◽  
Ali Reza Vakili ◽  
Reza Valizadeh ◽  
Abdollah Ghasemi Pirbalouti
Keyword(s):  
Essential Oils ◽  
Dairy Cow ◽  
Dry Matter ◽  
Basal Diet ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Plant Essential Oils ◽  
Microbial Nitrogen ◽  
Vitro Effect

Present study was conducted to investigate the effect of including plant essential oils on in vitro ruminal fermentation and microbial nitrogen synthesis of a dairy cow diet rich in concentrate. The treatments consisted of the diet alone (control; BD) as well as containing 50 and 100 μl L-1 essential oil of thyme (BDT), mint (BDM), savory (BDS), or a mixture of the essential oils at the rate of 1:1:1 (BDmix). Essential oils decreased gas production at 24, 48 and 96 h of incubation compared with that of BD. However, mint at the rate of 50 or 100 μl L-1 resulted an increase in the microbial nitrogen when compared to BD, BDS and BDT. The nitrogen content of truly undegraded residu (NDFN) content and NH3-N concentration were lower, while the dry matter digestibility was greater in the BDmix, regardless of dosage levels, as compared with the control. The inclusion of a mixture of essential oils at 50 μl L-1 to the basal diet caused intensified dry matter disappearance, in comparison to other treatments. Results showed that the synergetic effects of essential oils together in a dairy cow diet of rich in concentrate can alter rumen microbial fermentation and improve microbial protein yield.

scholarly journals Partial substitution of fish oil for linseed oil enhances beneficial fatty acids from rumen biohydrogenation but reduces ruminal fermentation and digestibility in growing goats

2021 ◽  
Author(s):  
Lam Phuoc Thanh ◽  
Noppharat Phakachoed ◽  
Wisitiporn Suksombat ◽  
Juan J Loor ◽  
Tran Thi Thuy Hang
Keyword(s):  
Fish Oil ◽  
Linseed Oil ◽  
Latin Square ◽  
Basal Diet ◽  
Partial Substitution ◽  
Partial Replacement ◽  
Minimal Risk ◽  
Ruminal Fermentation ◽  
Growing Goats

Abstract This study was performed to investigate effects of partial replacement of fish oil (FO) for linseed oil (LO) on digestibility, ruminal fermentation and biohydrogenation in growing goats. Experiment 1 was carried out in four growing male goats aged 6 months in a 4 × 4 Latin square design. Goats were fed a basal diet supplemented with 25 g/kg dry matter either LO alone or in combination with tuna FO. Treatments were developed by replacing FO for LO at ratios of 0, 5, 10 and 15 g/kg DM corresponding to FO-0, FO-5, FO-10 and FO-15, respectively. Experiment 2 was carried out in an in vitro incubation system including 12 fermenters with the same four treatments. Each fermenter consisted of 40 mL goat ruminal fluid, 160 mL warm buffer, 2 g mixed substrates, and 50 mg FO-0, FO-5, FO-10 or FO-15. Fish oil inclusion reduced (P &lt; 0.05) digestibility and nitrogen retention in Exp. 1. Increasing doses of FO in the diet induced a strong drop (P &lt; 0.001) in ruminal total volatile fatty acid (VFA) concentration and protozoa population at 3 h post incubation, but did not affect individual VFA proportions. Substitution of FO for LO decreased mean concentrations of C18:0 (P = 0.057), c-9,c-12 C18:2 and C18:3n-3 (P &lt; 0.001), but increased (P &lt; 0.001) C20:5n-3 and C22:6n-3. Feeding FO-10 enhanced formation of ruminal c-9,t-11 conjugated linoleic acid (CLA) concentration compared with FO-0. Overall, combined data suggest that to improve ruminal concentrations of C20:5n-3, C22:6n-3, and c-9,t-11 CLA for deposition in tissues or milk with minimal risk of affecting digestibility and ruminal fermentation, a dietary supplementation of 15 g/kg LO and 10 g/kg FO would be suitable.

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