INFLUENCE OF PRE-SOWING TREATMENT OF SEEDS WITH COMPOSITIONS OF  METABOLICALLY ACTIVE SUBSTANCES ON THE CONTENT OF PHOTOSYNTHETIC  PIGMENTS IN THE LEAVES OF WINTER RYE PLANTS OF SYNTHETIC 38 AND ZABAVA  VARIETIES AT DIFFERENT STAGES OF ONTOGENESIS

Purpose. The aim of the study is to investigate the effect of pre-sowing treatment of winter rye seeds of Synthetic 38 and Zabava with compositions of metabolically active substances on the content of photosynthetic pigments in plant leaves at different phases of ontogenesis. Materials and methods. The research materials were winter rye varieties Synthetic 38 and Zabava, and combinations of metabolically active substances: vitamin E (10-8M), paraoxybenzoic acid (0,001%), methionine (0,001%), ubiquinone-10 (10-8M) and MgSO4 (0,001%). The research scheme provided 4 options: 1) control (untreated seeds); 2) seeds, treated with a composition of substances: vitamin E + paraoxybenzoic acid + methionine + MgSO4; 3) seeds treated with a composition of substances: vitamin E + paraoxybenzoic acid + methionine; 4) seeds, processing by composition of substances: vitamin E + ubiquinone-10. Studies were conducted in the following phases of rye development: tillering, tubing, earing, flowering. The content of pigments - chlorophyll a, b and the total content of chlorophyll in the leaves of rye plants was determined by spectrophotometric method. Results and conclusions. It was demonstrated for the first time that the use of compositions of metabolically active compounds for pre-sowing treatment of winter rye varieties Synthetic 38 and Zabava leads to an increase of main chlorophyll, chlorophyll a and b in plant leaves in the dynamics from tillering to flowering. The composition consisting of vitamin E, para-oxybenzoic acid, methionine and MgSO4 showed the greatest efficiency. The effectiveness of this composition may be due to the complex action of all metabolically active compounds that are part of it. The obtained results may indicate a potentially more efficient absorption and conversion of energy by plants and the positive effect of these compositions on the photosynthesis of winter rye plants. Substances that have shown their effectiveness can be used as components of stimulants.

Download Full-text

EFFECT OF FOLIAR FERTILIZATION AND SOME GROWTH REGULATORS ON THE CHEMICAL COMPOSITION AND THE PHOTOSYNTHETIC PIGMENTS OF CROTON PLANT LEAVES

HortScience ◽

10.21273/hortsci.25.9.1101e ◽

1990 ◽

Vol 25 (9) ◽

pp. 1101e-1101

Author(s):

Mahmoud R. Shedeed ◽

Khairy M. El-Gamassy ◽

Alaa M. Almulla

Keyword(s):

Chemical Composition ◽

Chlorophyll A ◽

Growth Regulators ◽

Photosynthetic Pigments ◽

Potassium Content ◽

Plant Leaves ◽

Foliar Fertilization ◽

Chlorophyll A And B ◽

Phosphorus And Potassium ◽

Nitrogen Phosphorus

The experiment was designed to investigate the effect of Fulifertil at levels 0,2 and 4 gm/L and growth regulators GA3 at 0,100,200 and 300 ppm also kinetin at 0,25,50 and 75 ppm and their combination on croton plants. In general the results indicate that there were increases in the carbohydrates, nitrogen, phosphorus and potassium content. The anthocyanins and carotenoids pigments were increased while chlorophyll `a” and “b” pigments were decreased. These findings are required to give colorful leaves which give good display.

Download Full-text

Variations of Chlorophyll Content at Abies alba and Nepeta pannonica Species According to Phenophase and Harvesting Area

Cercetari Agronomice in Moldova ◽

10.2478/cerce-2019-0008 ◽

2019 ◽

Vol 52 (1) ◽

pp. 74-78

Author(s):

S. Buhăianu ◽

Doina Carmen Jităreanu

Keyword(s):

Chlorophyll Content ◽

Chlorophyll A ◽

Photosynthetic Pigments ◽

Photosynthetic Activity ◽

Abies Alba ◽

Chlorophyll B ◽

Green Color ◽

Yellow Coloration ◽

Chlorophyll A And B ◽

Chlorophyll A Content

Abstract Chlorophylls from plants are photosynthetic pigments. Their quantity offers valuable informations about photosynthetic activity, growing and developing of plants. Photosynthetic pigments decrease quantitatively during senescence process or in stress conditions. The present study has been realized in laboratory conditions with material harvested from spontaneous flora. The purpose of this research was the investigation of variations of chlorophyll content from samples of biological material collected from Nepeta pannonica L. and Abies alba Mill. plants, from Câmpulung Moldovenesc and Cacica areas, Suceava county, Romania. The targeted phenophases were growth and flowering. There were realized acetonic extracts from samples for spectrophotometric determinations. Obtained data were processed to estabilish chlorophyll a and b content. There were observed that at Abies alba species, from both locations, the chlorophyll a content grew during flowering phenophase, while the chlorophyll b content had little variations. At Nepeta pannonica species, the chlorophyll a and b content decreased visibly during the flowering, due to stress. Leaves of plants from this species presented a intense green color in the growing phenophase, while during flowering phenophase they had a purple or yellow coloration. Obtained results revealed a different dynamics of chlorophyll content at studied species.

Download Full-text

EFECTO DE LA FUENTE DE LUZ SOBRE LA MICROPROPAGACIÓN DE PLANTAS DEORÉGANO MEXICANO(Lippia graveolens)

e-CUCBA ◽

10.32870/e-cucba.v0i15.179 ◽

2021 ◽

Vol 15 (8) ◽

pp. 44-52

Author(s):

Sánchez-Velázquez Emmanuel ◽

◽

Rodríguez-Sahagún AracelI ◽

Acevedo-Hernández Gustavo J ◽

Rayn C Aarland ◽

...

Keyword(s):

Chlorophyll A ◽

Photosynthetic Pigments ◽

Broad Spectrum ◽

Energy Demand ◽

The Other ◽

Light Sources ◽

Similar Response ◽

Led Light ◽

Fluorescent Lamps ◽

Chlorophyll A And B

The use of different light sources in the micropropagation of plant species has been studied since the 1970s. At present, studies are being carried out not only to increase production, but to greatly reduce the energy demand of the lamps that are used for this purpose. In the present work, light sources with different wavelengths in fluorescent lamps and LED light were used to test the morphological and phytochemical response of Mexican origanumplants. A similar response was obtained regarding the production of axillary buds and leaves with the use of broad spectrum light and LED light. On the other hand, the main photosynthetic pigments (chlorophyll a and b and carotenoids) are also produced in a similar way when this plant is propagated under broad spectrum light and LED light.

Download Full-text

Productivity of triticale depending on the content of photosynthesizing pigments at anthesis

PLANT AND SOIL SCIENCE ◽

10.31548/agr2021.02.015 ◽

2021 ◽

Vol 12 (2) ◽

pp. 15-22

Author(s):

B.O. Mazurenko ◽

Keyword(s):

Chlorophyll Content ◽

Chlorophyll A ◽

Photosynthetic Pigments ◽

Dry Matter ◽

Nitrogen Fertilizers ◽

Chlorophyll B ◽

Varietal Response ◽

Chlorophyll A And B ◽

Fertilizer System

Photosynthetic pigments play an important role in the accumulation of dry matter and they can be the markers of stress. Change in the ratio of chlorophyll A and B indicates physiological changes and adaptation of the organism to changes of environmental conditions. Sowing terms and application of nitrogen fertilizers are long-term factors, so the content of chlorophylls in the flag leaf indicates long-term adaptation of the photosynthetic system. Establishing a relationship between the chlorophyll content at anthesis and the accumulation of dry matter in posy-anthesis period indicates the varietal response of triticale to fertilization and sowing dates. It was conducted a three-factor field experiment. There are studied two winter and one facultative triticale cultivar, two autumn sowing terms and fertilization system with application the different rates of nitrogen fertilizers in different growth stages. It was found that the content of photosynthetic pigments in each variety varied depending on sowing term and fertilizer system in terms of mg per 1 g of dry matter for chlorophyll A, B and the amount of A+B. At the same time, the difference between the factors and their interactions was insignificant for the total chlorophyll mass per unit area (g/m2) for chlorophyll A and the amount of A+B, but it was significant for chlorophyll B by the fertilizer system factor. This indicates that the main stress signal is chlorophyll B, so the ratio of chlorophyll A and B differed significantly depending on this factor. Cla: Clb ratio in the optimal sowing period is 5.3–8.1 in cv. Pidzimok kharkivskiy, 4.8–8.3 in cv. Amur and 5.0–6.7 in cv. Obriy mironivskiy. All cultivars have a strong positive correlation between the chlorophyll content at anthesis and accumulation of dry matter at post-anthesis perion under optimal conditions, but facultative triticale Pidzimok kharkivskiy has a negative correlation with unfavorable, due to increased chlorophyll, without increasing dry matter accumulation. Further study of the relationship between chlorophyll content and the synthesis of primary and secondary metabolites is promising in the study of varietal response to stress and agronomic factors.

Download Full-text

ACÚMULO DE PARTICULADOS ATMOSFÉRICOS E SUA INFLUÊNCIA NOS PIGMENTOS FOTOSSINTÉTICOS DE DUAS ESPÉCIES ARBÓREAS NA CIDADE DE PATOS-PB, BRASIL

FLORESTA ◽

10.5380/rf.v47i4.48075 ◽

2017 ◽

Vol 47 (4) ◽

pp. 365

Author(s):

Jorge Danilo Zea-Camaño ◽

Patrícia Carneiro Souto ◽

Antonio Lucineudo De Oliveira Freire ◽

Jacob Silva Souto ◽

Romualdo Medeiros Cortez Costa ◽

...

Keyword(s):

Chlorophyll A ◽

Photosynthetic Pigments ◽

Azadirachta Indica ◽

Urban Areas ◽

Urban Forest ◽

Pearson Correlation ◽

Clear Trend ◽

Atmospheric Particulates ◽

Chlorophyll A And B ◽

The City

Partículas atmosféricas contaminantes em áreas urbanas associadas principalmente com a queima de combustíveis fosseis se depositam, em parte, na superfície das folhas das árvores. Isso promove alterações físicas nas plantas que podem ser analisadas por meio de suas respostas metabólicas. Este estudo objetivou avaliar a eficiência no acúmulo de particulados atmosféricos pela folhagem, assim como determinar a influência dos particulados nos pigmentos fotossintéticos clorofila a e b e carotenoides em árvores de Tabebuia aurea (Silva Manso) Benth. & Hook. f. ex S. Moore e Azadirachta indica A. Juss. localizadas em praças públicas na cidade de Patos-PB. Foram selecionadas árvores localizadas em dez praças públicas com alto e baixo fluxo veicular. O acúmulo de particulados atmosféricos nas folhas das árvores foi quantificado em laboratório e correlacionado com os resultados do teor dos pigmentos fotossintéticos. A determinação dos teores de clorofila a e b e carotenoides foi realizada segundo o método de Lichtenthaler e Buschmann com leitura em espectrofotômetro. Foi aplicado o teste t (p < 0,05) para comparação de médias e calculado o coeficiente de correlação de Pearson para avaliar as interações entre as variáveis. Os resultados evidenciam que a espécie T. aurea é mais eficiente do que A. indica na captura de partículas contaminantes suspensas no ar através de sua folhagem. Não foi demostrada uma tendência clara da influência do acúmulo de particulados atmosféricos na clorofila a e b e carotenoides, assim como da influência do fluxo veicular na retenção de particulados atmosféricos por ambas espécies.Palavras-chave: Poluição atmosférica, fotossíntese, arborização urbana, serviços ecossistêmicos, semiárido brasileiro. AbstractAccumulation of atmospheric particulates and their influence on the photosynthetic pigments of two tree species in the city of Patos-PB, Brazil,..Contaminant atmospheric particles in urban areas associated with the burning of fossil fuels are deposited in part on the surface of the trees’ leaves; this promotes physical changes in the plants that can be analyzed through their metabolic responses. This study aimed to evaluate the efficiency of the accumulation of atmospheric particulates in the foliage as well as to determine the influence of the particulates in the photosynthetic pigments chlorophyll a and b and carotenoids in Tabebuia aurea (Silva Manso) Benth. & Hook. f. ex S. Moore and Azadirachta indica A. Juss. located in public squares, in the city of Patos-PB. Trees located in public places with both high and low vehicle traffic were selected. The accumulation of atmospheric particulates in the trees’ leaves was quantified in laboratory and correlated with the results of the content of the photosynthetic pigments. The determination of the levels of chlorophyll a and b and carotenoids was performed according to the method of Lichtenthaler and Buschmann with a spectrophotometer reading. The t test was used (p <0.05) to compare means and the Pearson correlation coefficient was calculated to evaluate the interactions between the variables. The results show that the T. aurea species is more efficient than A. indica at capturing contaminating particles suspended in the air through its foliage. A clear trend of the influence of the accumulation of atmospherics particulates on chlorophyll a and b and carotenoids has not been demonstrated, as well as the influence of the vehicular flow on the retention of atmospheric particulates by both species. Keywords: Air pollution, photosynthesis, urban forest, ecosystem services, Brazilian semiarid.

Download Full-text

ВПЛИВ ПЕРЕДПОСІВНОЇ ОБРОБКИ НАСІННЯ МЕТАБОЛІЧНО АКТИВНИМИ РЕЧОВИНАМИ НА ОКРЕМІ ФІЗІОЛОГІЧНІ ПОКАЗНИКИ СОЇ СОРТУ АННУШКА ТА ЇЇ ПРОДУКТИВНІСТЬ

Scientific Issue Ternopil Volodymyr Hnatiuk National Pedagogical University. Series: Biology ◽

10.25128/2078-2357.20.1-2.12 ◽

2020 ◽

Vol 79 (1-2) ◽

pp. 84-90

Author(s):

A. G. Koziuchko ◽

V. M. Havii ◽

O. B. Kuchmenko

Keyword(s):

Vitamin E ◽

Magnesium Sulfate ◽

Seed Treatment ◽

Lateral Roots ◽

Control Group ◽

Soybean Plant ◽

Number Of Leaves ◽

Ubiquinone 10 ◽

Active Substances ◽

Number Of Seeds

Soy is an important food, feed and technical crop of agriculture in many countries across the world. It differs from other agricultural crops by its unique combination of protein and oil with valuable vitamins and elements. Without the use of fertilizers, it is impossible to get a large soybean crop. Among the main factors that influence the yield of this crop, fertilizers account for 30%, varieties -20%, weather conditions and plant protection - 15%, effective fertility and soil treatment - 10%. Therefore, the study of the effect of a combination of metabolically active substances (complexes of vitamin E and ubiquinone-10; vitamin E, 4-hydroxibenzoic acid (PHBE) and methionine; vitamin E, 4-hydroxibenzoic acid (PHBE), methionine and magnesium sulfate on individual physiological parameters of soy in the main phases of ontogenesis and its performance is worthy of scientific attention. The research demonstrated that in the phase of formation of the triple leaf, the studied substances effectively stimulated the length of the main stem, the number of leaves and the area of the leaf surface. In the flowering phase, complexes of vitamin E and ubiquinone-10, and vitamin E, PHBE, methionine and magnesium sulfate increased the number of lateral roots by 132.2% and 114.3%, respectively. In addition, the studied metabolically active substances positively affected the length of the stem, number of leaves, leaf area and length of lateral roots. In the maturation phase, the complex of vitamin E and ubiquinone-10 effectively stimulated the height of plants, the number of beans per plant, exceeding the control parameters by 15.5%, 14.2%, respectively. In addition, the studied substances affected the number of seeds per soybean plant. So, in the control group, the number of seeds from one plant averaged 140.7 units. The best results were obtained using the combination of vitamin E, PHBE, methionine and magnesium sulfate and amounted to 154.4 units, which exceeded the control indices by 9.7%. While the number of seeds from one plant after treatment with a combination of vitamin E and ubiquinone-10 amounted to 148.3 units, which exceeded the control indicators by 5.4%. The highest soybean yield was observed during seed treatment with a combination of vitamin E and ubiquinone-10, and amounted to 2.37 t / ha, exceeding the control indicators by 26.3%. Seed treatment with complex of vitamin E, PHBE, methionine and magnesium sulfate also showed a good result, exceeding the control indicators by 10.2%. Thus, the complexes of vitamin E and ubiquinone-10, and vitamin E, PHBE, methionine and magnesium sulfate showed better results, so further study of the effect of these substances on legumes is promising.

Download Full-text

An extended PROSPECT: Advance in the leaf optical properties model separating total chlorophylls into chlorophyll a and b

Scientific Reports ◽

10.1038/s41598-017-06694-y ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 8

Author(s):

Yao Zhang ◽

Jingfeng Huang ◽

Fumin Wang ◽

George Alan Blackburn ◽

Hankui K. Zhang ◽

...

Keyword(s):

Optical Properties ◽

Chlorophyll A ◽

Leaf Optical Properties ◽

Chlorophyll A And B

Download Full-text

A study of the low-lying singlet and triplet electronic states of chlorophyll A and B

Journal of the Serbian Chemical Society ◽

10.2298/jsc130806096e ◽

2013 ◽

Vol 78 (11) ◽

pp. 1775-1787 ◽

Cited By ~ 5

Author(s):

Mihajlo Etinski ◽

Milena Petkovic ◽

Miroslav Ristic

Keyword(s):

Chlorophyll A ◽

Density Functional ◽

Chlorophyll B ◽

Fluorescence Lifetimes ◽

Functional Theory ◽

Chlorophyll A And B ◽

Vertical Ionization Potentials ◽

Density Functional Theory Optimization ◽

Triplet Formation ◽

Good Agreement

Chlorophylls have been extensively investigated both experimentally and theoretically owing to the fact that they are essential for photosynthesis. We have studied two forms of chlorophyll, chlorophyll a and chlorophyll b, by means of density functional theory. Optimization of S0, S1 and T1 states was performed with the B3-LYP functional. The computed fluorescence lifetimes show good agreement with the available experimental data. The electronic adiabatic energies of S1 and T1 states are 2.09/2.12 and 1.19/1.29 eV for chlorophyll a and chlorophyll b respectively. We discussed the implications of this results on the triplet formation. Also, the calculated vertical ionization potentials shows good agreement with the experimental results.

Download Full-text

Light-Induced Accumulation and Stability of Chlorophylls and Chlorophyll-Proteins during Chloroplast Development in Radish Seedlings

Zeitschrift für Naturforschung C ◽

10.1515/znc-1981-5-614 ◽

1981 ◽

Vol 36 (5-6) ◽

pp. 421-430 ◽

Cited By ~ 37

Author(s):

H. K. Lichtenthaler ◽

G. Burkard ◽

G. Kuhn ◽

U. Prenzel

Keyword(s):

Chlorophyll A ◽

Early Stage ◽

Continuous Darkness ◽

Suitable Parameter ◽

Chlorophyll Accumulation ◽

Chlorophylls A And B ◽

Sds Page ◽

Chlorophyll Protein ◽

Chlorophyll A And B ◽

Radish Seedlings

Abstract Illumination of 3 day old etiolated radish seedlings with continuous white light results in a progressive accumulation of chlorophyll a and b. Both pigments are bound in a different way to the thylakoid chlorophyll-proteins, which appear parallel to the formation of chlorophylls. By applying the SDS-PAGE method to SDS-digested chloroplasts, it was possible to show that the chloroplasts of radish cotyledons contain the typical chlorophyll proteins LHCP1-3, CPa, CPI and CPIa which have been found in other plants. Between LHCP1 and CPI an additional chlorophyll protein is detected with the spectral properties of a LHCP; it is termed here LHCPy. When the green plants are transferred to continuous darkness, chlorophylls and the chlorophyll-proteins are progressively degraded. At an early stage of greening chlorophyll b is destroyed at a much higher rate in darkness than chlorophyll a, which yields high chlorophyll a/b ratios. This is paralleled by a faster decrease in the level of the corresponding chlorophyll a/b-protein LHCP3 than of CPI. At a later stage of greening, after the end of the logarithmic chlorophyll accumulation, the chlorophylls a and b and also the LHCP3 and CPI are destroyed in continuous darkness at equal rates; the a/b ratios and the LHCP3/CPI ratios are then little different from the light control. The data indicate that at an early stage of greening the light-harvesting chlorophyll a/b-protein LHCP3 is less stable than the other chlorophyll-proteins (CPI, CPIa, CPa), which contain pre dominantly chlorophyll a. The ratio chlorophyll a to β-carotene (a/c ratio) of CPIa, CPI and CPa is about 10, while that of the LHCP1-3 is found to be between 150 to 300. We therefore propose using the a/c ratio to define the chlorophyll-proteins which, besides the absorption spectra, is the most suitable parameter.

Download Full-text

Technical Note: Multispectral lidar time series of pine canopy chlorophyll content

Biogeosciences ◽

10.5194/bg-12-1629-2015 ◽

2015 ◽

Vol 12 (5) ◽

pp. 1629-1634 ◽

Cited By ~ 18

Author(s):

T. Hakala ◽

O. Nevalainen ◽

S. Kaasalainen ◽

R. Mäkipää

Keyword(s):

Chlorophyll A ◽

Point Cloud ◽

Laser Scanning ◽

Technical Note ◽

Tree Canopy ◽

Tree Level ◽

Spatial Changes ◽

New Instrument ◽

Leaf Level ◽

Chlorophyll A And B

Abstract. We present an empirical application of multispectral laser scanning for monitoring the seasonal and spatial changes in pine chlorophyll (a + b) content and upscaling the accurate leaf-level chlorophyll measurements into branch and tree level. The results show the capability of the new instrument for monitoring the changes in the shape and physiology of tree canopy: the spectral indices retrieved from the multispectral point cloud agree with laboratory measurements of the chlorophyll a and b content. The approach opens new prospects for replacing destructive and labour-intensive manual sampling with remote observations of tree physiology.

Download Full-text