Evaluation of acute toxicity of vinasse by means of Daphnia magna and Aliivibrio fischeri: a comparative study

Abstract In the bioethanol industry, per liter of the produced alcohol 9 to 14 liters of vinasse are obtained as a byproduct. If the vinasse is directly shed into bodies of water without an adequate treatment, it may have negative effects on the existing biota and human health due to its high turbidity and color, low pH and high content of organic material. The purpose of this study was to assess the acute toxicity of vinasse by means of a rapid test with Aliivibrio fischeri and compare it with a standard immobilization assay with Daphnia magna. The standard assay of D. magna by means of its EC50 of 4.7% showed that organism was more sensitive to the contaminant, in comparison with the 69.6% obtained with the A. fischeri which suggests that it should be continuesly used as one of the organisms of first choice for the evaluation of the acute toxicity of this effluent.

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Correlation between acute toxicity for Daphnia magna, Aliivibrio fischeri and physicochemical variables of the leachate produced in landfill simulator reactors

Environmental Technology ◽

10.1080/09593330.2017.1281352 ◽

2017 ◽

Vol 38 (22) ◽

pp. 2898-2906 ◽

Cited By ~ 2

Author(s):

José J. Barrios Restrepo ◽

Letícia Flohr ◽

Silvia P. Melegari ◽

Cristina H. da Costa ◽

Cristiane F. Fuzinatto ◽

...

Keyword(s):

Acute Toxicity ◽

Daphnia Magna ◽

Physicochemical Variables ◽

Aliivibrio Fischeri

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Sensitivity of Zebrafish (Danio rerio) Embryos to Hospital Effluent Compared to Daphnia magna and Aliivibrio fischeri

Physiological Research ◽

10.33549/physiolres.934616 ◽

2020 ◽

pp. S681-S691

Author(s):

M WITTLEROVÁ ◽

G JÍROVÁ ◽

A VLKOVÁ ◽

K KEJLOVÁ ◽

M MALÝ ◽

...

Keyword(s):

Acute Toxicity ◽

Daphnia Magna ◽

Sublethal Effects ◽

Aquatic Organisms ◽

Spinal Curvature ◽

Treated Wastewater ◽

Zebrafish Embryos ◽

Fish Embryo ◽

Additional Tool ◽

Aliivibrio Fischeri

The Fish Embryo Acute Toxicity (FET) Test was adopted by the Organisation for Economic Co-operation and Development as OECD TG 236 in 2013. The test has been designed to determine acute toxicity of chemicals on embryonic stages of fish and proposed as an alternative method to the Fish Acute Toxicity Test performed according to OECD TG 203. In recent years fish embryos were used not only in the assessment of toxicity of chemicals but also for environmental and wastewater samples. In our study we investigated the acute toxicity of treated wastewater from seven hospitals in the Czech Republic. Our main purpose was to compare the suitability and sensitivity of zebrafish embryos with the sensitivity of two other aquatic organisms commonly used for wastewater testing – Daphnia magna and Aliivibrio fischeri. For the aim of this study, in addition to the lethal endpoints of the FET test, sublethal effects such as delayed heartbeat, lack of blood circulation, pericardial and yolk sac edema, spinal curvature and pigmentation failures were evaluated. The comparison of three species demonstrated that the sensitivity of zebrafish embryos is comparable or in some cases higher than the sensitivity of D. magna and A. fischeri. The inclusion of sublethal endpoints caused statistically significant increase of the FET test efficiency in the range of 1-12 %. Based on our results, the FET test, especially with the addition of sublethal effects evaluation, can be considered as a sufficiently sensitive and useful additional tool for ecotoxicity testing of the acute toxicity potential of hospital effluents.

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Acute toxicity of organic chemicals to Gammarus pulex correlates with sensitivity of Daphnia magna across most modes of action

Aquatic Toxicology ◽

10.1016/j.aquatox.2011.02.002 ◽

2011 ◽

Vol 103 (1-2) ◽

pp. 38-45 ◽

Cited By ~ 48

Author(s):

Roman Ashauer ◽

Anita Hintermeister ◽

Eva Potthoff ◽

Beate I. Escher

Keyword(s):

Acute Toxicity ◽

Daphnia Magna ◽

Gammarus Pulex ◽

Organic Chemicals ◽

Modes Of Action

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Didymo and Its Polysaccharide Stalks: Beneficial to the Environment or Not?

Polysaccharides ◽

10.3390/polysaccharides2010005 ◽

2021 ◽

Vol 2 (1) ◽

pp. 69-79

Author(s):

Hurmat Ejaz ◽

Esther Somanader ◽

Uday Dave ◽

Hermann Ehrlich ◽

M. Azizur Rahman

Keyword(s):

Invasive Species ◽

Structural Component ◽

Negative Effects ◽

Adhesive Properties ◽

The Past ◽

Positive Side ◽

Potential Benefits ◽

Negative Impacts ◽

Bodies Of Water ◽

Agricultural Setting

Didymosphenia geminata diatoms, or Didymo, was first found to be an invasive species that could have negative impacts on the environment due to the aggressive growth of its polysaccharide-based stalks. The stalks’ adhesive properties have prompted park officials to alert the general public to limit further spread and contamination of this algae to other bodies of water. Although the negative effects of Didymo have been studied in the past, recent studies have demonstrated a potential positive side to this alga. One of the potential benefits includes the structural component of the polysaccharide stalks. The origin of the polysaccharides within stalks remains unknown; however, they can be useful in a waste management and agricultural setting. The primary purpose of this study was to describe both the harmful and beneficial nature of Didymo. Important outcomes include findings related to its application in various fields such as medicine and technology. These polysaccharides can be isolated and studied closely to produce efficient solar power cells and batteries. Though they may be harmful while uncontained in nature, they appear to be very useful in the technological and medical advancement of our society.

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The assessment of Ficaria verna acute toxicity by two alternative methods: Daphnia magna and Physella acuta bioassays

Toxicology Letters ◽

10.1016/j.toxlet.2016.06.1323 ◽

2016 ◽

Vol 258 ◽

pp. S66

Author(s):

O. Olaru ◽

G. Nitulescu ◽

C. Spinu ◽

I. Potolea ◽

O. Pirvu ◽

...

Keyword(s):

Acute Toxicity ◽

Daphnia Magna ◽

Alternative Methods ◽

Physella Acuta ◽

Ficaria Verna

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Investigating the process of electrocoagulation in the removal of azo dye from synthetic textile effluents and the effects of acute toxicity on Daphnia magna test organisms

Journal of Water Process Engineering ◽

10.1016/j.jwpe.2021.102485 ◽

2022 ◽

Vol 45 ◽

pp. 102485

Author(s):

Carlos Eduardo Lach ◽

Camila Schwarz Pauli ◽

Aline Scheller Coan ◽

Edesio Luiz Simionatto ◽

Luciano André Deitos Koslowski

Keyword(s):

Acute Toxicity ◽

Daphnia Magna ◽

Azo Dye ◽

Textile Effluents ◽

Test Organisms

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Toxicity of five anilines to crustaceans, protozoa and bacteria

Journal of the Serbian Chemical Society ◽

10.2298/jsc091219103s ◽

2010 ◽

Vol 75 (9) ◽

pp. 1291-1302 ◽

Cited By ~ 21

Author(s):

Mariliis Sihtmäe ◽

Monika Mortimer ◽

Anne Kahru ◽

Irina Blinova

Keyword(s):

Daphnia Magna ◽

Vibrio Fischeri ◽

Environmental Pollutants ◽

Industrial Effluents ◽

General Tendency ◽

Test Species ◽

Mechanisms Of Toxicity ◽

Aliivibrio Fischeri ◽

Integrated Project ◽

The Eu

Aromatic amines (anilines and related derivates) are an important class of environmental pollutants that can be released to the aquatic environment as industrial effluents or as breakdown products of pesticides and dyes. The toxicities of aniline, 2-chloroaniline, 3-chloroaniline, 4-chloroaniline and 3,5-dichloroaniline towards a multitrophic test battery comprised of bacteria Aliivibrio fischeri (formerly Vibrio fischeri), a ciliated protozoan Tetrahymena thermophila and two crustaceans (Daphnia magna and Thamnocephalus platyurus) were investigated. Under the applied test conditions, the toxicities of the anilines notably varied among the test species. The bacteria and protozoa were much less sensitive towards the anilines than the crustaceans: EC50 values 13-403 mg L-1 versus 0.13-15.2 mg L-1. No general tendency between toxicity and the chemical structure of the anilines (the degree of chloro-substitution and the position of the chloro-substituents) was found in the case of all the tested aquatic species. The replacement of the artificial test medium (ATM) by the river water remarkably decreased the toxicity of anilines to crustaceans but not to protozoa. This research is part of the EU 6th Framework Integrated Project OSIRIS, in which ecotoxicogenomic studies of anilines (e.g., for Daphnia magna) will also be performed that may help to clarify the mechanisms of toxicity of different anilines.

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Acute toxicity of some insecticides on Artemia salina and Daphnia magna

Journal of Biotechnology ◽

10.1016/j.jbiotec.2012.07.081 ◽

2012 ◽

Vol 161 ◽

pp. 29

Author(s):

Dilsad Onbasili ◽

Fatih Duman ◽

Berrak Altinsoy ◽

Hatice Bekci

Keyword(s):

Acute Toxicity ◽

Daphnia Magna ◽

Artemia Salina

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Application of Biotests in Cyanobacterial Extract Toxicity Assessment

Archives of Environmental Protection ◽

10.2478/aep-2014-0028 ◽

2014 ◽

Vol 40 (3) ◽

pp. 115-121 ◽

Cited By ~ 1

Author(s):

Anna Sierosławska ◽

Anna Rymuszka ◽

Tadeusz Skowroński

Keyword(s):

Acute Toxicity ◽

Daphnia Magna ◽

Microcystis Aeruginosa ◽

Tetrahymena Thermophila ◽

Toxicity Assessment ◽

Taxonomic Identification ◽

Dam Reservoir ◽

Extract Concentration ◽

Initial Extract ◽

Toxicity Unit

Abstract The aim of the study was to determine the toxicity of the extract obtained from the cyanobacterial cells derived from the waters of Zemborzycki dam reservoir with use of a battery of biotests. The taxonomic identiﬁcation of the bloom-forming cyanobacteria revealed high abundance of Aphanizomenon ﬂos-aquae and Dolichospermum spp. (Anabaena spp.) and in a lower degree of Microcystis aeruginosa and Planktothrix agardhii. In the extract obtained from concentrated cyanobacterial cells, hepatotoxin microcystin-LR at a concentration of 22.89 ± 3.74 μg/L and neurotoxin Antx-a at 13.02 ± 0.01 μg/L have been detected. Toxicity of the extract was evaluated with the following assays: Daphtoxkit F magna with the crustacean Daphnia magna, Thamnotoxkit F with the crustacean Thamnocephalus platyurus, Rotoxkit F with the rotifer Brachionus calyciﬂorus and Protoxkit F with ciliate Tetrahymena thermophila. The most sensitive organism among all studied was T. platyurus for which EC50 was estimated to be 1.2% of the initial extract concentration. On the basis of the highest obtained value of the toxicity unit (TU = 83) the studied sample was classiﬁed to the IV class, which is of high acute toxicity. Additionally, it was found that reactivity on cyanobacterial products differs greatly among organisms used in bioassays, which indicate the need for using a set of biotests.

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