scholarly journals Genetic characterisation of African swine fever virus in outbreaks in Ha Nam province, Red River Delta Region of Vietnam, and activity of antimicrobial products against virus infection in contaminated feed

2020 ◽  
Vol 64 (2) ◽  
pp. 207-213
Author(s):  
Ha Thi Thanh Tran ◽  
Anh Duc Truong ◽  
Duc Viet Ly ◽  
Thi Hao Vu ◽  
Van Tuan Hoang ◽  
...  

AbstractIntroductionAfrican swine fever (ASF) was officially reported in Vietnam in February 2019 and spread across the whole country, affecting all 63 provinces and cities.Material and MethodsIn this study, ASF virus (ASFV) VN/Pig/HaNam/2019 (VN/Pig/HN/19) strain was isolated in primary porcine alveolar macrophage (PAM) cells from a sample originating from an outbreak farm in Vietnam’s Red River Delta region. The isolate was characterised using the haemadsorption (HAD) test, real-time PCR, and sequencing. The activity of antimicrobial feed products was evaluated via a contaminated ASFV feed assay.ResultsPhylogenetic analysis of the viral p72 and EP402R genes placed VN/Pig/HN/19 in genotype II and serogroup 8 and related it closely to Eastern European and Chinese strains. Infectious titres of the virus propagated in primary PAMs were 106 HAD50/ml. Our study reports the activity against ASFV VN/Pig/HN/19 strain of antimicrobial Sal CURB RM E Liquid, F2 Dry and K2 Liquid. Our feed assay findings suggest that the antimicrobial RM E Liquid has a strong effect against ASFV replication. These results suggest that among the Sal CURB products, the antimicrobial RM E Liquid may have the most potential as a mitigant feed additive for ASFV infection. Therefore, further studies on the use of antimicrobial Sal CURB RM E Liquid in vivo are required.ConclusionsOur study demonstrates the threat of ASFV and emphasises the need to control and eradicate it in Vietnam by multiple measures.

2014 ◽  
Vol 46 (4) ◽  
pp. 691-696 ◽  
Author(s):  
Nguyen Thi Lan Anh ◽  
Dao Thi Ha Thanh ◽  
Doan Huu Hoan ◽  
Do Thu Thuy ◽  
Nguyen Viet Khong ◽  
...  

2021 ◽  
Vol 226 (14) ◽  
pp. 146-152
Author(s):  
Vũ Thị Thu Hường ◽  
Cao Việt Hà ◽  
Ngô Văn Giới

Nghiên cứu tập trung vào đánh giá hiện trạng và biến động diện tích, năng suất, mức độ thâm canh và hiệu quả kinh tế của các loại hình sử dụng đất lúa tại Đồng bằng sông Hồng. Nghiên cứu được thực hiện bằng các phương pháp thu thập và kế thừa tài liệu, điều tra nông hộ, đánh giá hiệu quả kinh tế của quá trình sử dụng đất. Kết quả nghiên cứu cho thấy, năm 2018 toàn vùng có 588.357 ha, chiếm 15,04% diện tích đất lúa cả nước. Trong 10 năm (2010 - 2018), diện tích đất lúa của vùng giảm 31.589 ha, với tốc độ giảm bình quân 3.948,6 ha/năm. Hầu hết các tỉnh (10/11 tỉnh) đều giảm diện tích đất lúa (trừ tỉnh Quảng Ninh), bình quân giảm từ 143 – 800 ha/năm, trong đó thành phố Hà Nội giảm nhiều nhất. Mức độ thâm canh lúa cao, hệ số sử dụng đất lúa thay đổi từ 1,85 lần (2010) đến 1,77 lần (2018). Năng suất lúa toàn vùng giai đoạn 2010 - 2012 tăng và từ 2012 - 2018 có dấu hiệu giảm. LUT5 có hiệu quả kinh tế cao nhất, hiệu quả thấp nhất là LUT2.


2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Tran Quang Binh ◽  
Pham Tran Phuong ◽  
Bui Thi Nhung ◽  
Do Dinh Tung

2021 ◽  
Vol 14 (3) ◽  
pp. 794-802
Author(s):  
Quang Lam Truong ◽  
Lan Thi Nguyen ◽  
Haig Yousef Babikian ◽  
Rajeev Kumar Jha ◽  
Hoa Thi Nguyen ◽  
...  

Background and Aim: African swine fever is one of the severe pathogens of swine. It has a significant impact on production and economics. So far, there are no known remedies, such as vaccines or drugs, reported working successfully. In the present study, the natural oil blend formulation's (NOBF) efficacy was evaluated against ASFV in vitro using porcine alveolar macrophages (PAMs) cells of swine. Materials and Methods: The capacity of NOBF against the ASFV was tested in vitro. The NOBF combines Eucalyptus globulus, Pinus sylvestris, and Lavandula latifolia. We used a 2-fold serial dilution to test the NOBF formulation dose, that is, 105 HAD50/mL, against purified lethal dose of African swine in primary PAMs cells of swine. The PAM cells survival, real-time polymerase chain reaction (PCR) test, and hemadsorption (HAD) observation were performed to check the NOBF efficacy against ASFV. Results: The in vitro trial results demonstrated that NOBF up to dilution 13 or 0.000625 mL deactivates the lethal dose 105 HAD50 of ASFV. There was no HAD (Rosetta formation) up to dilution 12 or 0.00125 mL of NOBF. The Ct value obtained by running real-time PCR of the NOBF group at 96 h post-infection was the same as the initial value or lower (25), whereas the Ct value of positive controls increased several folds (17.84). Conclusion: The in vitro trial demonstrated that NOBF could deactivate the ASFV. The NOBF has the potential to act as anti-ASFV agent in the field. The next step is to conduct in vivo level trial to determine its efficacy.


2018 ◽  
Vol 10 (10) ◽  
pp. 3826 ◽  
Author(s):  
An Ha Truong ◽  
Minh Thuy Kim ◽  
Thi Thu Nguyen ◽  
Ngoc Tung Nguyen ◽  
Quang Trung Nguyen

Livestock farming is a major source of greenhouse gas and ammonia emissions. In this study, we estimate methane, nitrous oxide and ammonia emission from livestock sector in the Red River Delta region from 2000 to 2015 and provide a projection to 2030 using IPCC 2006 methodologies with the integration of local emission factors and provincial statistic livestock database. Methane, nitrous oxide and ammonia emissions from livestock farming in the Red River Delta in 2030 are estimated at 132 kt, 8.3 kt and 34.2 kt, respectively. Total global warming potential is estimated at 5.9 MtCO2eq in 2030 and accounts for 33% of projected greenhouse gas emissions from livestock in Vietnam. Pig farming is responsible for half of both greenhouse gases and ammonia emissions in the Red River Delta region. Cattle is another major livestock responsible for greenhouse gas emissions and poultry is one that is responsible for ammonia emissions. Hanoi contributes for the largest emissions in the region in 2015 but will be surpassed by other provinces in Vietnam by 2030.


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