scholarly journals Rapid flow injection electrochemical detection of 3,3′,4,4′ tetrachlorobiphenyl using stabilized lipid membranes with incorporated sheep antibody

2013 ◽  
Vol 11 (2) ◽  
pp. 320-323 ◽  
Author(s):  
Dimitrios Nikolelis ◽  
Nikolas Psaroudakis ◽  
Antonis Michaloliakos ◽  
Georgia-Paraskevi Nikoleli ◽  
Michael Scoullos
Keyword(s):  
Detection Limit ◽  
Ethylene Glycol ◽  
Electrochemical Detection ◽  
Flow Injection ◽  
Lipid Membranes ◽  
Electrochemical Biosensor ◽  
Glycol Dimethacrylate ◽  
Lipid Film ◽  
Rapid Flow ◽  
Number Of Cycles

AbstractAn electrochemical biosensor based on a supported polymerized lipid film with incorporated sheep anti-3,3′,4,4′ tetrachlorobiphenyl (PCB congener 77) antibody using flow injection analysis was developed. The polymerized lipid film contained 85% (w/w) dipalmitoylphosphatidylcholine (DPPC) and 15% (w/w) dipalmitoylphosphatidic acid (DPPA), methacrylic acid, ethylene glycol dimethacrylate, AIBN and sheep anti-congener 77 antiserum. Congener 77 was injected into flowing carrier electrolyte and the flow stopped to detect the antigen. These membranes gave only a single transient proportional to log [congener 77] from 10−8 to 10−5 M, with a detection limit of ca. 10−8 M. A membrane containing 35% (w/w) DPPA was used to examine regeneration. The maximum number of cycles was about 5.

Rapid Flow Injection Electrochemical Detection of Arochlor 1242 Using Stabilized Lipid Membranes with Incorporated Sheep anti-PCB Antibody

2011 ◽  
Vol 24 (3) ◽  
pp. 495-501 ◽  
Author(s):  
Antonis I. Michaloliakos ◽  
Georgia-Paraskevi Nikoleli ◽  
Christina G. Siontorou ◽  
Dimitrios P. Nikolelis
Keyword(s):  
Electrochemical Detection ◽  
Flow Injection ◽  
Lipid Membranes ◽  
Rapid Flow

Competitive heterogeneous enzyme immunoassay for theophylline by flow-injection analysis with electrochemical detection of p-aminophenol

1990 ◽  
Vol 36 (4) ◽  
pp. 662-665 ◽  
Author(s):  
E P Gil ◽  
H T Tang ◽  
H B Halsall ◽  
W R Heineman ◽  
A S Misiego
Keyword(s):  
Alkaline Phosphatase ◽  
Detection Limit ◽  
Electrochemical Detection ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
General Procedure ◽  
Amperometric Detection ◽  
Enzyme Label ◽  
Commercial Kit

Abstract A competitive enzyme-linked immunoabsorbent assay based on the flow-injection amperometric detection of p-aminophenol has been investigated with use of the materials and general procedure of a commercial kit for the determination of theophylline in human serum. The antibody is immobilized on glass beads, and the enzyme label is alkaline phosphatase (EC 3.1.3.1). The high currents generated during the electrochemical detection allowed a rapid (35 min) and simple determination of theophylline throughout its therapeutic range (10-20 mg/L) and also in the subtherapeutic range (detection limit of about 80 micrograms/L).

scholarly journals Determination of Thiocyanate by Kinetic Spectrophotometric Flow Injection Analysis

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Mohsen Keyvanfard ◽  
Khadijeh Alizad ◽  
Parinaz Elahian
Keyword(s):  
Detection Limit ◽  
Hydrochloric Acid ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Calibration Graph ◽  
Well Water ◽  
Janus Green ◽  
Rapid Flow ◽  
Kinetic Spectrophotometric

A simple and rapid flow injection spectrophotometric method has been developed for the determination of amount of thiocyanate in wastewaters and well water samples. It is based on the reaction of thiocyanate in hydrochloric acid with janus green and bromate. Reaction was monitored spectrophotometrically by measuring janus green absorbance atλmax=554 nm. The calibration graph was linear over the range 0.02–1.0 μg mL−1and the detection limit was 0.016μg mL−1(s/n=3). The throughput was 25 samples per hour.

scholarly journals PREPARATION OF A NEW MOLECULARLY IMPRINTED POLYMERS AND ITS USE IN THE SELECTIVE EXTRACTION FOR DETERMINATION BROMHEXINE HYDROCHLORIDE AT PHARMACEUTICALS

2019 ◽  
Vol 50 (3) ◽  
Author(s):  
Mahdi & et al.
Keyword(s):  
Detection Limit ◽  
Ethylene Glycol ◽  
Molecularly Imprinted Polymers ◽  
Pharmaceutical Analysis ◽  
Life Time ◽  
Trisodium Citrate ◽  
Selective Extraction ◽  
Glycol Dimethacrylate ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

This study was aimed four electrodes were synthesized based on molecularly imprinted polymers (MIPs). Two MIPs were prepared by using bromhexine hydrochloride (BHH) as the template, acryl amide (AA) and methyl methacrylate (MMA) as monomers as well as ethylene glycol dimethacrylate (EGDMA) and penta erythritol triacrylate (PETA) as cross linkers respectively and benzoyl peroxide as initiator. The same composition was used in preparation of non-imprinted polymers (NIPs), but without the template (Bromhexine hydrochloride). To prepare the membranes, different plasticizers were used in PVC matrix such as: Di butyl sebacate (DBS), acetophenone (AP), di-octyl phthalate (DOPH) and tri-2-ethyl hexyl phosphate (TEHP). The characteristics studied are the slop, detection limit, life time and linearity range of BHH–MIPs electrodes. Results obtained of selectivity measurements on interfering cations (Al+3, Ca+2, K+) and some pharmaceutical additives such as methylparaben, propylparaben, trisodium citrate show that no interfering with drug bromhexine hydrochloride. The preparation electrodes have been shown good response including testing pharmaceutical analysis.

Crosslinked Poly (4-Vinylpyridine-Ethylene Glycol Dimethacrylate) Used for Preconcentration of Cd(II) and its Determination by Flow Injection Flame Atomic Absorption Spectrometry

2014 ◽  
Vol 97 (2) ◽  
pp. 605-611 ◽  
Author(s):  
César Ricardo Teixeira Tarley ◽  
Natália Cristina Botteon Farias ◽  
Giovana de Fátima Lima ◽  
Fernanda Midori de Oliveira ◽  
Rudy Bonfílio ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Flow Rate ◽  
Flow Injection ◽  
Flame Atomic Absorption Spectrometry ◽  
Water Samples ◽  
Absorption Spectrometry ◽  
Glycol Dimethacrylate ◽  
Flame Atomic Absorption

Abstract The main purpose of this research was to synthesize crosslinked poly(4-vinylpyridine-ethylene glycol dimethacrylate) and evaluate its feasibility for highly sensitive and selective determination of Cd in water samples by using flow injection flame atomic absorption spectrometry. The crosslinked polymer, prepared by bulk polymerization, was characterized by FTIR spectrometry and scanning electron microscopy. The flow injection solid-phase method was based on preconcentration of 20.0 mL of sample through 100 mg of the polymer packed into a minicolumn at pH 8.25 using a flow rate of 6.0 mL/min, followed by elution with 1.0 M HNO3. The sample solution parameters influencing the preconcentration behavior of Cd ions, such as pH, buffer concentration, and flow rate, were simultaneously studied and optimized using a Doehlert matrix. Values of 0.10 μg/L, 2.0–210 μg/L, 32.3, 18/h, 9.7/min, and 0.62 mL were obtained for LOD, linear range, preconcentration factor, sample throughput, concentration efficiency, and consumption index, respectively. The effect of the presence of the inorganic cations Pb(II), U(IV), Co(II), Hg(II), Cu(II), As(III), Mg(II), Sb(III), Ni(II), Th(IV), Ba(II), and Ca(II) on the method was studied, and the preconcentration of Cd was observed to have no interference. The accuracy of the method was assessed by analysis of natural water samples using addition and recovery tests and inductively coupled plasma/MS as a reference technique, as well as by analysis of a standard reference material of trace elements in water.

scholarly journals Determination of Manganese in Feedstuffs by Flow Injection Spectrophotometry Based on Rapid Formation of Permanganate at Room Temperature

2001 ◽  
Vol 84 (4) ◽  
pp. 1011-1016 ◽  
Author(s):  
Jing-Fu Liu ◽  
Ying-Di Feng ◽  
Gui-Bin Jiang
Keyword(s):  
Standard Deviation ◽  
Detection Limit ◽  
Flow Injection ◽  
Room Temperature ◽  
Sampling Rate ◽  
Relative Standard ◽  
Rapid Flow ◽  
Rapid Formation ◽  
Optimized Conditions

Abstract A simple and rapid flow injection spectrophotometric procedure was developed for determination of manganese. In the presence of pyrophosphate and acetate, manganese was immediately oxidized to permanganate by periodate at room temperature in slightly alkaline medium. Under optimized conditions, the determination was made with a sampling rate of 120/h, a linear range of 0–30 mg/L Mn(II), a detection limit (S/N = 3) of 0.08 mg/L, and a relative standard deviation of 0.6% (n = 11) at 10 mg/L Mn(II). The proposed method was used to determine manganese in trace mineral premixes and feedstuffs. Results agreed well with those obtained by the standard atomic absorption spectroscopy method.

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