Airlift reactor — membrane extraction hybrid system for aroma production

2013 ◽  
Vol 67 (12) ◽  
Author(s):  
Mário Mihaľ ◽  
Sean Gavin ◽  
Jozef Markoš
Keyword(s):  
Hybrid System ◽  
Hollow Fiber Membrane ◽  
Fermentation Medium ◽  
Product Inhibition ◽  
Airlift Reactor ◽  
Membrane Extraction ◽  
Production Methods ◽  
Operational Conditions ◽  
Yield Increase ◽  
Aroma Production

AbstractIn recent times, environmental production methods and organic products are increasingly sought after in food, perfume, and cosmetic industries, where the products are consumed or come into direct contact with humans. One such additive is 2-phenylethanol, an alcoholic aromatic rose like smell compound, mainly used as a flavor and aroma. 2-Phenylethanol can be produced by bioconversion from l-phenylalanine using Saccharomyces cerevisiae. This type of biotransformation is strongly limited by product inhibition which allows reaching the maximum concentration of 2-phenylethanol, 4 g L−1, in an ordinary batch, fed-batch, or chemostat bioreactor. The main aim of the presented work was to study the possible yield increase of 2-phenylethanol in a hybrid system consisting of membrane extraction performed by a hollow fiber membrane module immersed in the downcomer of an airlift reactor. Such hybrid system can be used to remove 2-phenylethanol from the fermentation medium and thus to overcome the product inhibition of biotransformation. In this paper, the influence of biomass on membrane extraction of 2-phenylethanol from aqueous solution in an airlift reactor to alkanes at different operational conditions was studied. The measured extraction kinetics was compared with the predictions obtained by a mathematical model. Hydrodynamics of the hybrid system was also studied.

Intensive 2-phenylethanol production in a hybrid system combined of a stirred tank reactor and an immersed extraction membrane module

2014 ◽  
Vol 68 (12) ◽  
Author(s):  
Mário Mihaľ ◽  
Ramiro Goncalves ◽  
Jozef Markoš
Keyword(s):  
Hybrid System ◽  
Hollow Fiber Membrane ◽  
Water Solution ◽  
Product Inhibition ◽  
Membrane Module ◽  
Membrane Extraction ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Operational Conditions ◽  
Biotransformation Process

AbstractBioconversion of l-phenylalanine to 2-phenylethanol using Saccharomyces cerevisiae is connected with the growth of biomass strongly limited by product inhibition. Therefore, fermentation can proceed only at low conversions of l-phenylalanine with very low yield of the desired product, which allows reaching the maximum concentration of 2-phenylethanol, 4 g L−1, in an ordinary batch, fed-batch, or chemostat bioreactor. To minimize capital and operating costs in the bioproduction of chemical specialties where the product inhibits the bioreaction, using a hybrid system based on the application of membrane extraction integrated in the bioreactor to remove the product is a suitable solution. Integration can be done by an external module for membrane extraction or, as a more efficient solution, by an extraction membrane module immersed directly in the bioreactor. Such a hybrid system can be used to remove 2-phenylethanol from the fermentation media and thus to overcome the product inhibition of the biotransformation process. In this paper, a hybrid system consisting of a stirred tank bioreactor (3.5 L) and an immersed extraction hollow fiber membrane module was studied. In the proposed system, the kinetics of 2-phenylethanol extraction from a water solution with and without biomass in the bioreactor to alkanes at different operational conditions was measured. Extraction kinetics was compared with the predictions obtained by a mathematical model. In the hybrid system, two extractive biotransformation experiments were performed and compared with that without product removal. Experimental data were also mathematically predicted with good accuracy between the simulation and the experiment.

Membrane extraction of 1-phenylethanol from fermentation solution

2011 ◽  
Vol 65 (2) ◽  
Author(s):  
Mário Mihaľ ◽  
Jozef Markoš ◽  
Vladimír Štefuca
Keyword(s):  
Mathematical Model ◽  
Organic Solvents ◽  
Hollow Fiber ◽  
Hollow Fiber Membrane ◽  
Membrane Extraction ◽  
Equilibrium System ◽  
Extraction Kinetics ◽  
Hollow Fiber Contactor ◽  
Experimental Values ◽  
Fermentation Solution

Abstract1-Phenylethanol can be produced by biotransformation of acetophenone using microorganisms. The next step is the separation of biomass from the fermentation solution (e.g. using microfiltration) and then the separation of the product. Membrane extraction was studied in the presented work for this purpose. Equilibria of acetophenone and 1-phenylethanol in the equilibrium system solute-organic solvent-water were investigated for three different organic solvents (heptane, toluene, ethyl acetate). On the basis of this investigation, extraction kinetics of both solutes from the model aqueous solution to the heptane organic phase, using a hollow fiber membrane module, were studied. To simulate the extraction kinetics, mathematical model of an experimental parallel flow hollow fiber contactor is presented and verified using experimental values with good agreement. Extraction kinetics for the investigated organic solvents were simulated and compared using the verified mathematical model and the chosen membrane extraction parameters.

Simulation and sensitivity analysis of transport in asymmetric hollow fiber membrane permeators for air separation

RSC Advances ◽  
2015 ◽  
Vol 5 (105) ◽  
pp. 86359-86370 ◽  
Author(s):  
Seyed Saeid Hosseini ◽  
Sara Najari ◽  
Prodip K. Kundu ◽  
Nicolas R. Tan ◽  
Seyed Mehrdad Roodashti
Keyword(s):  
Sensitivity Analysis ◽  
Hollow Fiber ◽  
Hollow Fiber Membrane ◽  
Air Separation ◽  
Fiber Membrane ◽  
Operational Conditions ◽  
Separation Membrane

Development of advanced membranes requires deep insights about the process. Sensitivity analysis is performed to analyze the effects of module properties and process operational conditions on the performance of air separation membrane permeators.

Membrane Extraction of Penicillin G from a Synthetic Fermentation Media Using Sunflower Oil

2010 ◽  
Vol 8 (1) ◽  
Author(s):  
Md Monwar Hossain
Keyword(s):  
Organic Phase ◽  
Sunflower Oil ◽  
Tributyl Phosphate ◽  
Hollow Fiber Membrane ◽  
Aqueous Media ◽  
Environmentally Friendly ◽  
Membrane Extraction ◽  
Penicillin G ◽  
Percentage Extraction ◽  
Fermentation Media

The use of sunflower oil as an environmentally-friendly solvent was examined for the separation of penicillin G (Pen G) from a synthetic fermentation media to develop a cost-effective process. In order to evaluate this, equilibrium experiments were first carried out with solvent alone (sunflower oil/pegasol/tributyl phosphate) and then with the solvent containing dissolved Amberlite LA-2, an ionic carrier. The organic systems with the carrier gave better distribution of Pen G between the aqueous and organic phases. The values of the distribution coefficient were greater for tributyl phosphate (TBP) than the other solvents at the natural pH of Pen G. Sunflower oil gave a good distribution ratio and it can be recommended as an attractive option because of its low cost, minimal toxicity and moderate performance. The effectiveness of the carrier-sunflower oil (organic phase) was evaluated in a hollow-fiber membrane contactor by performing experiments with the organic phase on the shell side and the feed inside the fiber. With a small amount of carrier (approx. 5%) in the organic phase and at the natural pH of penicillin G, a good percentage extraction (approx. 40-45%) was achieved. The percentage extraction decreased (approx. 30-35%) when penicillin G was prepared in a synthetic fermentation media instead of pure aqueous media. These results are similar to those reported by many investigators; the main difference is that, in this report, an environmentally-friendly solvent was used instead of toxic solvents used in the literature, such as kerosene.

scholarly journals Contribution of Grape Skins and Yeast Choice on the Aroma Profiles of Wines Produced from Pinot Noir and Synthetic Grape Musts

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 168
Author(s):  
Yifeng Qiao ◽  
Diana Hawkins ◽  
Katie Parish-Virtue ◽  
Bruno Fedrizzi ◽  
Sarah J. Knight ◽  
...  
Keyword(s):  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Fermentation Medium ◽  
Pinot Noir ◽  
Gas Chromatography Mass Spectrometry ◽  
Wine Quality ◽  
Aroma Profile ◽  
Aroma Production ◽  
Grape Skins ◽  
The Impact

The aroma profile is a key component of Pinot noir wine quality, and this is influenced by the diversity, quantity, and typicity of volatile compounds present. Volatile concentrations are largely determined by the grape itself and by microbial communities that produce volatiles during fermentation, either from grape-derived precursors or as byproducts of secondary metabolism. The relative degree of aroma production from grape skins compared to the juice itself, and the impact on different yeasts on this production, has not been investigated for Pinot noir. The influence of fermentation media (Pinot noir juice or synthetic grape must (SGM), with and without inclusion of grape skins) and yeast choice (commercial Saccharomyces cerevisiae EC1118, a single vineyard mixed community (MSPC), or uninoculated) on aroma chemistry was determined by measuring 39 volatiles in finished wines using headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography–mass spectrometry (GC-MS). Fermentation medium clearly differentiated the volatile profile of wines with and without yeast, while differences between EC1118 and MSPC wines were only distinct for Pinot noir juice without skins. SGM with skins produced a similar aroma profile to Pinot noir with skins, suggesting that grape skins, and not the pulp, largely determine the aroma of Pinot noir wines.

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