Release of cotyledonary shoots of pea (Pisum sativum) seedlings from inhibition as related to endogenous zeatin and ethylene and exogenous IAA and benzyladenine

Biologia ◽  
2007 ◽  
Vol 62 (1) ◽  
Author(s):  
Helena Fišerová ◽  
Jiří Šebánek ◽  
Jan Hradilík ◽  
Petr Doležel ◽  
Zuzana Mikušová ◽  
...  

AbstractThis paper deals with apical dominance using a dicotylar model obtained after decapitation of pea seedlings with two shoots — one dominant and the other inhibited. When the dominant shoot was decapitated the inhibited one is released from inhibition and after 24 to 72 h begins to grow. However, the levels of trans-zeatin and production of ethylene increase within 4 and 6 hours respectively after release from inhibition, and within an interval of 72 h the levels of both phytohormones begin gradually to decrease. This indicates that also in this model, the release from apical dominance is associated with an increase in the level of cytokinin zeatin and, thereafter, also with an increased production of ethylene. If indolyl-3-acetic acid (IAA) is applied on the decapitated main stem after decapitation of the dominant shoot, the growth of the initially inhibited one is very strongly retarded; if, however, IAA is applied on the decapitated dominant shoot, this inhibition is significantly weaker. This means that the inhibiting effect of IAA on the inhibited shoot originates to a greater degree from the main stem rather than from the dominant shoot. The effect of benzyladenine (BA) is transferred equally from the decapitated main stem and from the decapitated dominant shoot because the initially inhibited shoot begins to grow as well as also other shoots from serial cotyledonary buds.

2011 ◽  
Vol 50 (No. 4) ◽  
pp. 182-187 ◽  
Author(s):  
Š. Klíčová ◽  
J. Šebánek ◽  
T. Vlašic

Flax seedlings were decapitated above the cotyledons. After one cotyledon was removed the growth of the bud of the remaining cotyledon was stronger in 90% of the plants. However, the application of the cytokinin benzyladenine (BA) to the bud of the removed cotyledon caused a growth correlative reversal and, by contrast, in 65% of the plants the bud of the removed cotyledon grew out. On the other hand, in sunflower seedlings, which have epigeal cotyledons similar to flax, after the removal of one cotyledon the growth of the axillary of the removed cotyledon was more intensive in 59% of the plants. Not even an application of BA to the remaining cotyledon of sunflower resulted in more intensive growth of the axillary of this cotyledon. When both cotyledons were left on the seedling, BA applied to one of the cotyledons of decapitated flax plants resulted in a highly significant stimulation of growth of the axillary of this cotyledon; in sunflower; however, the effect of the BA was insignificant. After decapitation of the stem of pea seedlings where both cotyledons remained, both axillaries grew out, but after a certain period of time one of them (the dominant one) achieved a growth correlative dominance over the other (inhibited). The present study is focused on whether an application of plant hormones onto the inhibited shoot is able to cause a growth correlative reversal, i.e. to change the inhibited shoot into a dominant one. The application of 0.12% BA can cause such a reversal virtually in all plants if the original difference in the length between the inhibited and dominating axillaries is 12–24 mm. A 0.12–0.5% concentration of gibberellin causes a reversal in 13–75% of the plants, but only if the difference between the dominant and inhibited shoot is 1–12 mm. A 0.03–0.25% concentration of IAA causes a reversal in 34–57% of the plants, if the difference in the length of the axillaries is 1–4 mm.


2011 ◽  
Vol 52 (No. 12) ◽  
pp. 559-563 ◽  
Author(s):  
H. Fišerová ◽  
J. Šebánek ◽  
J. Hradilík ◽  
S. Procházka

This study compares effects of 2,3,5-triiodobenzoic acid (TIBA) and flavonoid quercetine (Q). In spite of the fact that both these compounds are inhibitors of the polar transport of auxin, a number of experiments demonstrated that their properties are different. If the flax seedlings were decapitated and one cotyledon was removed, the axillary bud of the remaining cotyledon grew up more intensively while TIBA (0.5% applied in the form of a lanolin paste on the remaining cotyledon) induced a correlative reversal so that axillars of removed cotyledons grew up more intensively. However, when Q (0.5%) was applied on the remaining cotyledon in the form of a lanolin paste, this inhibition was not significant. In the lamina-deprived petioles of apple trees TIBA accelerated abscission while Q caused an inhibiting effect similar to that of auxin. TIBA applied on epicotyls of pea seedlings inhibited their growth by 35% while Q only by 15%. As far as the release of ethylene by pea seedlings is concerned, both compounds showed promoting effects similar to the effect of auxin.


For identification of varieties, it is desirable to use genetically predetermined traits that are decisive for the varietal identification of the distinguishing characteristics of the varieties production and new genotypes for selection. The presence a complete evaluation of the selection samples a particular crop is the basis for the formation of informative databases characteristics the varieties of standards used in plant examination to determine the level of expression a characteristic particular genotype according to the code of their manifestation. For the 20 traits we used to identify, we identified 6 least volatile, highly trait-stable traits over the years of study to identify. These are: “coloring of the pubescence of the main stem”, “the size of the lateral leaf”, “the intensity of green leaf color”, “flower color”, “the intensity of brown bean color”, “basic color of the seed coat”. The other 14 traits are highly variable and unstable among varieties, but may be significant under certain conditions. According to the results of the research we have determined the conformity of the collection varieties by code graduation. In the study, we selected varieties with corresponding codes of attributes of the reference varieties the UPOV technique. On the basic of the color the pubescence of the main stem, 36% varieties are with grey stem pubescence. Among the standards: Apache, Alaric, Talon according to the grey color of the pubescence, we identified varieties Zolotysta, Ozzie, Diona. The 2947 564/84, Stepnaya-90, Knyazhna. The lateral leaf size is a sign that affects the increase in green mass of plants and subsequently on productivity. Graduation of the sign small size of leaf had varieties: Zuma, Ksenya; middle – Heinong 37, Yuh-30; large – 2947 564/84, Vinni, DSS 2504. The following is a sign of the intensity of leaf plate, which is divided by gradation into light, moderate and dark. Among our samples with a light degree of color, we can distinguish Diona, Sribna Ruta, and Zuma. For varieties with moderate gradation of color are selected: Perlyna, 93/99, Knyazhna. To the dark color of the leaf plate we attributed the varieties: Stepnaya-90, Ozzie. Among the varieties we studied of the basics of flower color 37% varieties are with a white flower, and 63% with a purple. According to the varieties of standards: Chandor, Cresir, Toreador to the white color of the flower we attributed varieties Knyazhna, Ozzie, Zolotysta. With the purple color of the flower we have selected varieties: 93/99, Zhemchuzhna, Heinong 37. On the basis of the intensity of brown color bean, which characterizes the generative organs of the plant, is divided into weak, moderate and strong, but the other identifiers there are gradations: sandy, gray, light brown, brown and dark brown, so we decided what would be better identify by color gradation. To the sandy color of the beans we attributed varieties Perlyna, Yuh-30; gray – Sribna Ruta; light brown – Kobza, Knyazhna; brown – Ksenya; dark brown – Zhemchuzhna, Vinni. Important features that identify the description of varieties include basic color of the seed coat, which affects the taste of the seeds. In particular, the seeds are divided by color into yellow – Kobza, Yuh-30; yellow-green – Vinni, Luch Nadezhdy; green – Heorhina; light brown – 2947 564/84, brown – DSS 2504; dark brown – 2974 YS-24 and black color – not detected. Following the results of the previous collection of soybean varieties from the morphological sings of vegetative, generative organs of plants, it was possible to identify varieties with stable manifestation of standard identifying sings of soybean. Collection varieties with one standard characteristics: Heinong 37, Luch Nadezhdy, DSS 2504, 2974 YS-24; two signs: Zolotysta, Diona, Stepnaya-90, Zuma, Ksenya, Heorhina, Sribna Ruta, Perlyna, 93/99, Zhemchuzhna; three signs: Ozzie, 2947 564/84, Yuh-30, Vinni, Knyazhna.


Experiments were recently reported showing that, in young seedlings of Pisum sativum , the complete inhibiting effect which the shoot exerts upon its axillary buds comes entirely or almost entirely from three or four of its developing leave acting together (6). A single developing leaf was found usually to inhibit only partially—that is to say, sufficiently to delay the growth of an axillary bud below it, but not to check it completely. The strength of this partial inhibiting effect was measured by the retardation of the outgrowth of the axillary buds of the first or lowest leaf, as compared with their growth in completely defoliated controls. Comparisons were further made of the inhibiting effects of single young leaves of equal sizes near the apex in seedlings of different ages and heights, and it was found that in very young short seedlings the inhibiting effect was very slight or inappreciable, although in seedlings of a height of about 30 mm. or more (but still possessing well filled cotyledons) the effect was strong.


2013 ◽  
Vol 9 ◽  
pp. 8-14 ◽  
Author(s):  
Yan Sun ◽  
Jing Sun ◽  
Chao-Guo Yan

A fast and convenient protocol for the synthesis of novel spiro[dihydropyridine-oxindole] derivatives in satisfactory yields was developed by the three-component reactions of arylamine, isatin and cyclopentane-1,3-dione in acetic acid at room temperature. On the other hand the condensation of isatin with two equivalents of cyclopentane-1,3-dione gave 3,3-bis(2-hydroxy-5-oxo-cyclopent-1-enyl)oxindole in high yields. The reaction mechanism and substrate scope of this novel reaction is briefly discussed.


1972 ◽  
Vol 25 (10) ◽  
pp. 2107 ◽  
Author(s):  
GB Deacon ◽  
GD Fallon

Bismuth triarenesulphinates, Bi(02SR)3 [R = Ph, p-MeC6H4, p-ClC6H4, 2,4,6-(Me2CH)3C6H2, and p-MeCONHC6H4], have been prepared by reaction of bismuth triacetate with the appropriate arenesulphinio acids in glacial acetic acid, and the first two compounds have also been obtained by reaction of triphenyl-bismuth with the appropriate mercuric arenesulphinates. The sulphur-oxygen stretching frequencies of the bismuth sulphinates are indicative of O-sulphinate coordination, and the compounds are considered to be polymeric with bridging O-sulphinate groups and six-coordinate bismuth. Thermal decomposition of Bi(O2SR)3 (R = Ph, p-MeC6H4, or p-CIC6H4) under vacuum gave the corresponding triarylbismuth compounds and sulphur dioxide, the preparation of tri-p-chlorophenylbismuth being accompanied by formation of di-p-chlorophenyl sulphone and S-p-chlorophenyl p-chlorobenzenethiosulphonate. Pyrolysis of the other triarenesulphinates did not yield organobismuth compounds.


1970 ◽  
Vol 48 (7) ◽  
pp. 1347-1350 ◽  
Author(s):  
Pei-Show Juo ◽  
G. Stotzky

Globulins, albumins, and basic proteins were extracted from seeds of red kidney bean (Phaseolus vulgaris), and their distribution was in a ratio of about 3:2:1, respectively. The globulin fraction constituted a major portion of the reserve proteins and was hydrolyzed rapidly during germination. More than 90% of the basic proteins, extractable with 0.05 N acetic acid, disappeared 12 days after germination. Although the decrease in total albumin was not as marked as with the other two fractions, a number of components of this fraction disappeared during the early stages of germination, but several new components were detected about 8 days after germination. The apparent synthesis of new globulin components during germination was also observed, but no synthesis of basic protein could be detected.


1970 ◽  
Vol 33 (11) ◽  
pp. 516-520 ◽  
Author(s):  
T. E. Minor ◽  
E. H. Marth

The effect of gradually reducing the pH of pasteurized milk with acetic, citric, hydrochloric, lactic, and phosphoric acids over periods of 4, 8, and 12 hr on growth of Staphylococcus aureus 100 in this substrate was determined. In addition, 1: 1 mixtures of lactic acid and each of the other acids, and of acetic and citric acids were evaluated for their effect on growth of this organism. To achieve a 90% reduction in growth over a 12 hr period, a final pH value of 5.2 was required for acetic, 4.9 for lactic, 4.7 for phosphoric and citric, and 4.6 for hydrochloric acid. A 99% reduction during a 12 hr period was obtained with a final pH value of 5.0 for acetic, 4.6 for lactic, 4.5 for citric, 4.1 for phosphoric, and 4.0 for hydrochloric acid. A pH value of 3.3 was required for a 99.9% reduction with hydrochloric acid, whereas the same effect was produced at a pH value of 4.9 with acetic acid. Correspondingly lower pH values were required to inhibit growth within 8 and 4 hr periods. Mixtures of acids adjusted to pH values at the borderline for growth (12 hr period) exhibited neither synergistic nor antagonistic effects between two acids.


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