scholarly journals Monitoring of Biochemical Parameters in Preterm Infants on Special Regime Feeding

2007 ◽  
Vol 26 (2) ◽  
pp. 103-106
Author(s):  
Miroslava Ljujić-Glišić ◽  
Niveska Božinović-Prekajski
Keyword(s):  
Alkaline Phosphatase ◽  
Preterm Infants ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Feeding Regimes ◽  
Mother's Milk ◽  
Mother’S Milk ◽  
Biochemical Analyses ◽  
Growth Assessment ◽  
Biochemical Assessment

Monitoring of Biochemical Parameters in Preterm Infants on Special Regime FeedingEnriched human milk may stimulate gain weight in preterm infants during the neonatal period. Aim is the biochemical assessment of preterm infants, fed by mother's milk fortificated with the special domestic formulapreImpamil during the first month of life. 80 preterm infants (45 male and 35 female), up to 36 weeks of gestation, BW less than 2500 g, started enteral intake in the first three days of life. Total volume intake was in range from 70 mL/kg first day, to 170-200 mL/kg after a 10thday of life. Mother's milk fortification was prepared as a 5% mixture solution ofpreImpamil. The dynamics of biochemical analyses started on the first day of study and was repeated once a week. We analysed levels of: total protein, albumin, prealbumin, transferin, urea, Ca ionised, P and alkaline phosphatase using standard biochemical methods. Statistical analyses were completed by ANOVA test, one-factorial analyses of variance. During monitoring the total protein level increased in the second week of life (p<0.01), as well as albumin (p<0.01). Prealbumin level increased, as well as transferin (p-NS). Initial level of ionised fraction of Ca was significantly lower (p<0.01) at the beginning of the study, compared to the rest. Serum level of P increased, as well as the level of alkaline phosphatase at the end of first and second week (p<0.01). Conclusion is that biochemical parameters, such as levels of protein and albumin, are important in the growth assessment of preterm infants on special feeding regimes.

scholarly journals Biochemical and antropometric monitoring of growth in preterm infants

2009 ◽  
Vol 56 (2) ◽  
pp. 93-96
Author(s):  
M. Ljujic-Glisic ◽  
N. Bozinovic-Prekajski ◽  
M. Glibetic
Keyword(s):  
Alkaline Phosphatase ◽  
Preterm Infants ◽  
Total Protein ◽  
Neonatal Period ◽  
Initial Level ◽  
Feeding Regimes ◽  
Mother's Milk ◽  
Mother’S Milk ◽  
Biochemical Analyses ◽  
Biochemical Assessment

Enriched human milk may stimulate gain weight in preterm infants during neonatal period. Aim is biochemical assessment of preterm infants, feed by mother's milk fortificated with special domestic formula preImpamil during the first month of life. 80 preterm infants (45 male and 35 female), up to 36 weeks of gestation. BW less than 2500 g, which started enteral intake in the first three day of life. Total volume intake was in range from 70 ml/kg first day, to 170-200 ml/kg, after 10th day of life. Mother's milk fortification was prepared as 5% mixture solution of preImpamil. The dynamics of biochemical analyses started on the first day of study and was repeated once weekly. We analyzed levels of: total protein, albumin, prealbumin, transferin, urea, Ca ionised, P and alkaline phosphatase using standard biochemical methods. Statistical analyses completed by ANOVA test, one factorial analyses of variance. During monitoring total protein level increased in the second week of life (p<0,03), as well as albumin (p<0,03). Prealbumin level increased, as well as transferin (p-NS). Initial level of ionised fraction of Ca was significantly lower (p<0,03) at the beginning of the study, compared to the rest. Serum level of P increased, as well as level of alkaline phosphatase at the end of first and second week (p<0,01). Conclusion is that biochemical parameters as level of protein and albumin are important at the assessment growth in preterm infants on special feeding regimes.

Comparative assessment of biochemical parameters of Hariana, Hardhenu and Sahiwal breeds 

2018 ◽  
Author(s):  
Deepak Kumar ◽  
Sandeep Kumar ◽  
Sandeep Gera and Jyoti Yadav
Keyword(s):  
Alkaline Phosphatase ◽  
Potassium Chloride ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Comparative Assessment ◽  
Sodium Ion ◽  
Ion Concentration ◽  
Sodium Potassium ◽  
Aspartate Amino Transferase ◽  
Biochemical Assessment

Present study was conducted on seventy two cattle of three groups’ viz., Hardhenu strain of cross bred cattle, Sahiwal and Hariana breeds comprising twenty four animals of each group/breed. The biochemical assessment was based on assay of sodium, potassium, chloride, blood urea nitrogen (BUN), creatinine, glucose, calcium, total protein, albumin, alkaline phosphatase (ALP), alanine amino transferase (ALT), aspartate amino transferase (AST), bilirubin (total and direct) and triglycerides parameters. Triglycerides, ALP, creatinine and potassium ion concentration were found to be higher in Hariana while direct bilirubin, ALT and sodium ion concentration were comparatively higher in Hardhenu. However, Albumin, calcium and glucose were observed to be at higher concentration in Sahiwal.

scholarly journals The effects of different anticoagulants on biochemical parameters in blood of dairy cows

2015 ◽  
Vol 69 (1-2) ◽  
pp. 13-20
Author(s):  
Branislava Belic ◽  
Marko Cincovic ◽  
Maja Dosenovic ◽  
Dragica Stojanovic ◽  
Zorana Kovacevic
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Alkaline Phosphatase Activity ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Blood Parameters ◽  
Albumin Level ◽  
Blood Samples ◽  
Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

scholarly journals Toxic Effect of Ladosulfan-Pesticides on Biochemical Indices of Clarias Gariepinus (Burchell 1822) Juveniles

2020 ◽  
Vol 3 (3) ◽  
Keyword(s):  
Water Quality ◽  
Alkaline Phosphatase ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Clarias Gariepinus ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Commercial Formulation ◽  
Aspartate Amino Transferase ◽  
Biochemical Indices

ladosufan is an insecticide extensively used in agriculture for pest control. The 96h LC50 estimated from probeit analysis was 1.89mg/L. Sub lethal concentrations of 1/10th (0.19 mg/L),1/5th (0.49mg/L) and 1/2th (0.95mg/L) of LC50 were used to determine the effects of the ladosulfan -pesticide on biochemical parameters at day 1, 7, 14 and 21exposure periods. Water quality test was determined on pH, temperature, dissolved oxygen, hardness and alkalinity. The results show that biochemical parameters viz; Total Protein, Alkaline Phosphatase ALP, Alanine Transferase ALTAspartate Amino Transferase (AST) concentrations increased significantly (P ≤ 0.05) in time and concentration dependent manner. The biochemical parameters concentration had higher values in treated samples than control. The total protein increased from (8.80±0.002b -9.20±0.103b) at 0.19 mg/L, (9.20±0.102a –9.70±0.103c) at 0.48 mg/L and (9.27±0.152a –9.80±0.103c) at 0.95 mg/L of ladosulfan when compared with the control (7.67±0.931c –9.06±0.763c ); Alkaline Phosphatase increased from (60.00±0.002a -86.00±1.001b) at 0.19 mg/L, (62.00±1.002b –88.00±1.001c) at 0.48 mg/L and (65.00±1.001a –92.00±1.002d) at 0.95 mg/L of endosulfan when compared with the control (40.00±1.002a–53.67±5.511a) ; Alanine Transferase also increased from (30.00±1.002a -40.00±1.001c) at 0.19 mg/L, (35.00±1.002a –54.00±1.001a) at 0.48 mg/L and (38.00±1.002b –89.00±1.001d) at 0.95 mg/L of ladosulfan when compared with the control (22.33±2.083c –27.33±2.882b) ; Aspartate Amino Transferase increased from (64.00±1.002b -87.00±1.001c) at 0.19 mg/L, (66.00±1.002c –88.00±2.001d) at 0.48 mg/L and (68.33±0.582c –90.00±1.001d) at 0.95 mg/L of ladosulfan when compared with the control (63.00±2.002b –68.00±1.001b) ; respectively. The results indicate that commercial formulation of ladosulfan is toxic to C. gariepinus, the insecticide should be prudently used in both terrestrial and aquatic eco-systems to avoid eco-toxicological hazards.

249 BIOPHYSICAL AND BIOCHEMICAL CHARACTERISTICS OF ALPACA SEMEN AFTER COLLECTION BY ELECTROEJACULATION

2013 ◽  
Vol 25 (1) ◽  
pp. 272 ◽  
Author(s):  
J. Rodriguez ◽  
W. Huanca ◽  
M. Ramos ◽  
M. Vasquez ◽  
J. Espinoza
Keyword(s):  
Alkaline Phosphatase ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Density Lipoprotein ◽  
Alanine Transaminase ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Biochemical Characteristics

The collection of alpaca semen presents difficulties because of the characteristics of mounts and copulation time in addition to the high viscosity of semen. The biophysical and biochemical characteristics of alpaca semen were investigated. Semen samples from four adult males from 6 to 7 years of age were collected using an electroejaculation technique. The biophysical parameters measured in seminal plasma included volume, color, sperm concentration, motility, and viscosity, and the biochemical parameters included pH and glucose, calcium, triglyceride, cholesterol, high-density lipoprotein cholesterol, total protein, albumin, alanine transaminase, alkaline phosphatase, and γ-glutamyltranspeptidase concentrations. The mean time for semen collection was 10.3 ± 1.5 min; the volume varied from 1.2 to 3.8 and the colour from clear to milky, and pH was 7.4 ± 0.03. Mean concentration of spermatozoa was 69.3 ± 193 million mL–1, and motility was 52.4 ± 9.7%. Biochemical parameters were glucose (mg dL–1) 8.22 ± 0.77; cholesterol (mg dL–1) 79.78 ± 5.64; triglycerides (mg dL–1) 44.12 ± 7.38; high-density lipoprotein cholesterol (mg dL–1) 4.73 ± 0.30; total protein (g dL–1) 2.36 ± 0.15; albumin (g dL–1) 0.97 ± 0.33; calcium (mg dL–1) 11.77 ± 1.74; alanine transaminase (U L–1) 17.92 ± 9.09; alkaline phosphatase (U L–1) 288.76 ± 279.59; and γ-glutamyltranspeptidase (U L–1) 89.62 ± 39.09. These results provide information on the biophysical and biochemical characteristics of alpaca semen collected by electroejaculation, with variable results in the enzymatic components.

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