scholarly journals Toxic Effect of Ladosulfan-Pesticides on Biochemical Indices of Clarias Gariepinus (Burchell 1822) Juveniles

2020 ◽  
Vol 3 (3) ◽  
Keyword(s):  
Water Quality ◽  
Alkaline Phosphatase ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Clarias Gariepinus ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Commercial Formulation ◽  
Aspartate Amino Transferase ◽  
Biochemical Indices

ladosufan is an insecticide extensively used in agriculture for pest control. The 96h LC50 estimated from probeit analysis was 1.89mg/L. Sub lethal concentrations of 1/10th (0.19 mg/L),1/5th (0.49mg/L) and 1/2th (0.95mg/L) of LC50 were used to determine the effects of the ladosulfan -pesticide on biochemical parameters at day 1, 7, 14 and 21exposure periods. Water quality test was determined on pH, temperature, dissolved oxygen, hardness and alkalinity. The results show that biochemical parameters viz; Total Protein, Alkaline Phosphatase ALP, Alanine Transferase ALTAspartate Amino Transferase (AST) concentrations increased significantly (P ≤ 0.05) in time and concentration dependent manner. The biochemical parameters concentration had higher values in treated samples than control. The total protein increased from (8.80±0.002b -9.20±0.103b) at 0.19 mg/L, (9.20±0.102a –9.70±0.103c) at 0.48 mg/L and (9.27±0.152a –9.80±0.103c) at 0.95 mg/L of ladosulfan when compared with the control (7.67±0.931c –9.06±0.763c ); Alkaline Phosphatase increased from (60.00±0.002a -86.00±1.001b) at 0.19 mg/L, (62.00±1.002b –88.00±1.001c) at 0.48 mg/L and (65.00±1.001a –92.00±1.002d) at 0.95 mg/L of endosulfan when compared with the control (40.00±1.002a–53.67±5.511a) ; Alanine Transferase also increased from (30.00±1.002a -40.00±1.001c) at 0.19 mg/L, (35.00±1.002a –54.00±1.001a) at 0.48 mg/L and (38.00±1.002b –89.00±1.001d) at 0.95 mg/L of ladosulfan when compared with the control (22.33±2.083c –27.33±2.882b) ; Aspartate Amino Transferase increased from (64.00±1.002b -87.00±1.001c) at 0.19 mg/L, (66.00±1.002c –88.00±2.001d) at 0.48 mg/L and (68.33±0.582c –90.00±1.001d) at 0.95 mg/L of ladosulfan when compared with the control (63.00±2.002b –68.00±1.001b) ; respectively. The results indicate that commercial formulation of ladosulfan is toxic to C. gariepinus, the insecticide should be prudently used in both terrestrial and aquatic eco-systems to avoid eco-toxicological hazards.

Comparative assessment of biochemical parameters of Hariana, Hardhenu and Sahiwal breeds 

2018 ◽  
Author(s):  
Deepak Kumar ◽  
Sandeep Kumar ◽  
Sandeep Gera and Jyoti Yadav
Keyword(s):  
Alkaline Phosphatase ◽  
Potassium Chloride ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Comparative Assessment ◽  
Sodium Ion ◽  
Ion Concentration ◽  
Sodium Potassium ◽  
Aspartate Amino Transferase ◽  
Biochemical Assessment

Present study was conducted on seventy two cattle of three groups’ viz., Hardhenu strain of cross bred cattle, Sahiwal and Hariana breeds comprising twenty four animals of each group/breed. The biochemical assessment was based on assay of sodium, potassium, chloride, blood urea nitrogen (BUN), creatinine, glucose, calcium, total protein, albumin, alkaline phosphatase (ALP), alanine amino transferase (ALT), aspartate amino transferase (AST), bilirubin (total and direct) and triglycerides parameters. Triglycerides, ALP, creatinine and potassium ion concentration were found to be higher in Hariana while direct bilirubin, ALT and sodium ion concentration were comparatively higher in Hardhenu. However, Albumin, calcium and glucose were observed to be at higher concentration in Sahiwal.

scholarly journals The effects of different anticoagulants on biochemical parameters in blood of dairy cows

2015 ◽  
Vol 69 (1-2) ◽  
pp. 13-20
Author(s):  
Branislava Belic ◽  
Marko Cincovic ◽  
Maja Dosenovic ◽  
Dragica Stojanovic ◽  
Zorana Kovacevic
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Alkaline Phosphatase Activity ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Blood Parameters ◽  
Albumin Level ◽  
Blood Samples ◽  
Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

scholarly journals Modification of human placental alkaline phosphatase by periodate-oxidized 1,N6-ethenoadenosine monophosphate

1990 ◽  
Vol 272 (3) ◽  
pp. 683-690 ◽  
Author(s):  
G G Chang ◽  
M S Shiao ◽  
K R Lee ◽  
J J Wu
Keyword(s):  
Alkaline Phosphatase ◽  
Dependent Manner ◽  
Placental Alkaline Phosphatase ◽  
Phosphate Binding ◽  
Hydrophobic Region ◽  
Concentration Dependent Manner ◽  
General Chemical ◽  
Wide Range ◽  
Human Placental Alkaline Phosphatase ◽  
Activity Loss

Oxidation of 1,N6-ethenoadenosine monophosphate (epsilon AMP) with periodate cleaved the cis-diol of the ribose ring and resulted in the formation of a dialdehyde derivative (epsilon AMP-dial). At room temperature epsilon AMP-dial was unstable and underwent beta-elimination to give 4′,5′-anhydro-1,N6-ethenoadenosine dialdehyde acetal (A epsilon Ado-dial). These nucleotide analogues were found to inactivate human placental alkaline phosphatase in a time- and concentration-dependent manner. epsilon AMP-dial was shown to be an affinity label for the enzyme on the basis of the following criteria. (a) Kinetics of the enzyme activity loss over a wide range of epsilon AMP-dial concentration showed a saturating phenomenon. Removal of the phosphate group made the reagent (A epsilon Ado-dial) become a general chemical modifying reagent. (b) The artificial substrate p-nitrophenyl phosphate gave substantial protection of the enzyme against inactivation. (c) epsilon AMP-dial was a substrate and a partial mixed-type inhibitor for the enzyme. Results of the inhibition and protection studies indicated that the reagent and substrate could combine with the enzyme simultaneously. Besides the phosphate-binding domain, an induced hydrophobic region is proposed for the substrate-binding site for human placental alkaline phosphatase.

Effect of humic-plant feed preparations on biochemical blood parameters of laying hens in deep litter housing system

2015 ◽  
Vol 18 (1) ◽  
pp. 131-139 ◽  
Author(s):  
F. Bubel ◽  
Z. Dobrzański ◽  
A. Gaweł ◽  
K. Pogoda-Sewerniak ◽  
E.R. Grela
Keyword(s):  
Alkaline Phosphatase ◽  
Biochemical Parameters ◽  
Blood Parameters ◽  
Feed Additives ◽  
Housing System ◽  
Serum Total Protein ◽  
Humic Materials ◽  
Limited Degree ◽  
Biochemical Indices ◽  
Food Mixture

AbstractAn influence of various humic-plant feed additives based on some herbs (nettle, chamomile, yarrow, perforatum), lucerne and humic materials on biochemical indices of Lohmann Brown (LB) layers blood plasma was estimated. Hens were housed in deep litter system, 20 birds in a group. Four groups were formed: control (C – standard feeding), and experimental, supplemented with preparations: E-1 herbal-humic, E-2 humic-herbal and E-3 – humic-lucerne. Hens were placed in the pens on the 16thweek of life, addition of preparations with standard food mixture started at the 22ndwk and lasted until 66thwk of life. Blood for analyses was collected four times in the following periods: 27, 37, 54 and 65thwk of life. The applied humic-plant preparations to a limited degree affected the values of examined biochemical parameters in serum: total protein (TP), albumins (Albs), glucose (Glu), urea, triacylglycerols (TAG), total cholesterol (TCh), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). It is difficult to determine based on these study, which preparation is one the most active biologically, however is seems that humic-lucerne preparation affected the examined blood parameters to the highest degree. The reference values ranges in hens blood serum LB hens were proposed for: TP (43-65 g/l), Albs (15-22 g/l), urea (0.5-1.2 mmol/l), Glu 10-15 mmol/l), TCh (2.2-4.5 mmol/l), TAG (10-24 mmol/l), AST (4-12 U/l), ALT (150-280 mmol/l) and ALP (190-350 U/l).

scholarly journals Physiological and bacteriological profile of the fish Clarias gariepinus (Siluriformes: Clariidae) chronically exposed to different concentrations of municipal waste leachate in Nigeria

2019 ◽  
Vol 11 (2) ◽  
pp. 182-197
Author(s):  
Olufemi David Owolabi ◽  
Funmilola Janet Abioye ◽  
Patricia Folakemi Omojasola ◽  
Olufunbi Praise Aina
Keyword(s):  
Blood Cell ◽  
Cell Volume ◽  
Environmental Concern ◽  
Clarias Gariepinus ◽  
Haemoglobin Concentration ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Mean Cell Volume ◽  
Regulatory Limits ◽  
Bacteriological Profile

Introduction: One of the most commonly used methods of waste management is landfilling, which has been a major environmental concern. Objective: To examine the effect of leachate on Clarias gariepinus. Methods: We tested Clarias gariepinus (n=700) with varying concentrations of leachate (0, 10, 15, 20 and 25%) from the Amoyo dumpsite, Ilorin, Nigeria. After 28-day exposure, haematological, biochemical, bacteriological and histopathological assays were carried out. Results: pH ranged from 7,78 to 13,97; cadmium, iron, lead, manganese and zinc were 178-, 134-, 6248-, 400- and 3-fold, respectively, higher than the regulatory limits for waterbodies, while copper fell within the limits. Values of red blood cell, haemoglobin, packed cell volume, mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, lymphocytes and monocytes were significantly lower than the control. Conversely, white blood cell, neutrophils and eosinophils increased in a concentration-dependent manner. There were tissue-specific differences in the responses of all biochemical parameters investigated except for protein that significantly decreased in all the tissues. veitisiete bacterial isolates classified into seven genera including Bacillus, Klebsiella, Pseudomonas, Staphylococcus, Acinetobacter, Escherichia and Micrococcus were recorded from the leachate and selected organs of C. gariepinus. The total bacterial, viable coliform and total counts in the leachate were 4,2×106CFU/mL, 3,8×108CFU/mL, 3,6× 106CFU/mL and 2,7×108CFU/mL, respectively. The highest counts were recorded in the gills (14,2×106CFU/mL) and lowest in the muscle (2,7×106CFU/mL) at the highest and least concentration of the leachate, respectively. Most of the bacteria isolated from the leachate were also recovered from the fish organs. Escherichia coli had the highest frequency of occurrence (26%). Conclusion: This study revealed that toxic constituents of the leachate elicited deleterious changes in the bacteriology and physiology of C. gariepinus.

scholarly journals The Influence of Mg2+ Ions on the In Vitro Efficacy of Chitosan-Titanium Dioxide Nanotubes (CTNTs) Scaffolds

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Siew Shee Lim ◽  
Shui Wei Kho ◽  
Nehemiah Li Heng Ang ◽  
Cheng Heng Pang ◽  
Hwei-San Loh
Keyword(s):  
Alkaline Phosphatase ◽  
Titanium Dioxide ◽  
Weight Percent ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Titanium Dioxide Nanotubes ◽  
Mg63 Cells ◽  
Adsorption Affinity ◽  
Monolayer Adsorption

Low mechanical strength and lack of osteoconductive cues are problems associated with chitosan-based scaffolds. This research aimed to fabricate reinforced chitosan-titanium dioxide (TiO2) nanotubes (CTNTs) scaffolds attributed to the enhanced biocompatibility and physical properties of TiO2 nanotubes (TNTs). The incorporation of hydrothermally synthesized TNTs at weight percent of 16 into chitosan was achieved via direct blending and lyophilization. CTNTs scaffolds were further subjected to 24-h adsorption in MgCl2 solutions of 0.5 mM, 1 mM, 2.5 mM, and 5 mM at physiological pH. The adsorption affinity of CTNTs towards Mg2+ ions was high and mainly attributed to the macroporosity of scaffolds and nanocavities of TNTs. The maximum monolayer adsorption capacity of CTNTs for Mg2+ ions was 8.8 mg/g scaffolds. Its adsorption isotherm fitted well with Langmuir isotherm by showing R2 of 0.9995. Fluorescence-based staining, cell viability, and alkaline phosphatase assays indicated that the adsorbed Mg2+ ions onto CTNTs scaffolds aided in promoting higher proliferation and early differentiation of MG63 cells than scaffolds without Mg2+ ions in a concentration-dependent manner. Based on current results, CTNTs scaffolds with Mg2+ ions may be a potential biomaterial for bone regeneration.

249 BIOPHYSICAL AND BIOCHEMICAL CHARACTERISTICS OF ALPACA SEMEN AFTER COLLECTION BY ELECTROEJACULATION

2013 ◽  
Vol 25 (1) ◽  
pp. 272 ◽  
Author(s):  
J. Rodriguez ◽  
W. Huanca ◽  
M. Ramos ◽  
M. Vasquez ◽  
J. Espinoza
Keyword(s):  
Alkaline Phosphatase ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Density Lipoprotein ◽  
Alanine Transaminase ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Biochemical Characteristics

The collection of alpaca semen presents difficulties because of the characteristics of mounts and copulation time in addition to the high viscosity of semen. The biophysical and biochemical characteristics of alpaca semen were investigated. Semen samples from four adult males from 6 to 7 years of age were collected using an electroejaculation technique. The biophysical parameters measured in seminal plasma included volume, color, sperm concentration, motility, and viscosity, and the biochemical parameters included pH and glucose, calcium, triglyceride, cholesterol, high-density lipoprotein cholesterol, total protein, albumin, alanine transaminase, alkaline phosphatase, and γ-glutamyltranspeptidase concentrations. The mean time for semen collection was 10.3 ± 1.5 min; the volume varied from 1.2 to 3.8 and the colour from clear to milky, and pH was 7.4 ± 0.03. Mean concentration of spermatozoa was 69.3 ± 193 million mL–1, and motility was 52.4 ± 9.7%. Biochemical parameters were glucose (mg dL–1) 8.22 ± 0.77; cholesterol (mg dL–1) 79.78 ± 5.64; triglycerides (mg dL–1) 44.12 ± 7.38; high-density lipoprotein cholesterol (mg dL–1) 4.73 ± 0.30; total protein (g dL–1) 2.36 ± 0.15; albumin (g dL–1) 0.97 ± 0.33; calcium (mg dL–1) 11.77 ± 1.74; alanine transaminase (U L–1) 17.92 ± 9.09; alkaline phosphatase (U L–1) 288.76 ± 279.59; and γ-glutamyltranspeptidase (U L–1) 89.62 ± 39.09. These results provide information on the biophysical and biochemical characteristics of alpaca semen collected by electroejaculation, with variable results in the enzymatic components.

BIOCHEMICAL INDICES OF BLOOD SERUM OF RUBELLA-RESISTANT CARP BREED AT THE END OF THE FEEDING PERIOD

2021 ◽  
pp. 113-119
Author(s):  
D. Mikryakov ◽  
A. Revyakin ◽  
G. Pronina ◽  
A. Sokolova ◽  
A. Petrushin
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Total Protein ◽  
Feeding Period ◽  
Serum Samples ◽  
Mirror Carp ◽  
Chuvash Republic ◽  
Low Levels ◽  
Biochemical Indices ◽  
Papanin Institute

Biochemical parameters of blood serum in resistant and susceptible to rubella pathogens groups of carp at the end of the feeding period are studied. The material was taken from 6 individuals of the Angelina scaly rubella-resistant breed kept at the experimental pond base "Sunoga" of the Papanin Institute for Biology of Inland Waters RAS, Yaroslavl oblast. For comparison, we used same-age carps from the fish farm "Kirya", Chuvash Republic, susceptible to rubella pathogens: 8 specimens of scaly and 8 mirror carps. Blood samples were taken from the caudal vein of the fish under study. The tube with blood was left at room temperature. After coagulation, the serum was taken with a fine needle syringe and transferred to an Eppendorf tube. Serum samples were frozen prior to testing. The analyzed individuals of the Angelina rubella-resistant breed differ from the scaly and mirror carp in high levels of total protein, albumins, glucose, urea, alkaline phosphatase, triglycerides, cholesterol and low levels of lipids and alanine aminotransferase. A comparative analysis with previously obtained data after wintering shows differences between the autumn and spring data. In the Angelina breed, the levels of most indicators significantly increase during the fattening period. The content of total protein increases several times, mainly due to globulins, glucose, urea, alkaline phosphatase, triglycerides. Thus, the metabolic processes aimed at the accumulation of proteins, stored lipids and carbohydrates increase in the organism of rubella-resistant carps. This is probably due to some specific characteristics of the Angelina breed, which allows the fish organism to successfully resist an infection with rubella pathogens in spring. The results of this study can be used in breeding for immunity resistance and for assessing the health status of fish in aquaculture.

Effect of seasons on blood biochemical parameters in male dromedary camels in Algeria

2017 ◽  
Author(s):  
Brahim Hamad ◽  
Hebib Aggad ◽  
Leyla Hadef ◽  
Aicha Adaika
Keyword(s):  
Alkaline Phosphatase ◽  
Creatine Kinase ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
The Other ◽  
Dromedary Camels ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Aspartate Amino Transferase ◽  
Different Seasons

The present study aimed to investigate the effect of different seasons on blood biochemical parameters of male dromedary camels in Algeria. A total number of 30 clinically healthy camels from five to seven years old were sampled in this study and biochemical analysis were performed using an automatic analyzer. The obtained results showed that the urea, creatine kinase and alanine amino transferase concentrations increased significantly during summer compared to other seasons. However, lactate dehydrogenase concentration increased significantly during winter versus other seasons. On the other hand, no significant effect of season was found on glucose, triglyceride, cholesterol, creatinine, aspartate amino transferase and alkaline phosphatase concentrations.

scholarly journals Monitoring of Biochemical Parameters in Preterm Infants on Special Regime Feeding

2007 ◽  
Vol 26 (2) ◽  
pp. 103-106
Author(s):  
Miroslava Ljujić-Glišić ◽  
Niveska Božinović-Prekajski
Keyword(s):  
Alkaline Phosphatase ◽  
Preterm Infants ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Feeding Regimes ◽  
Mother's Milk ◽  
Mother’S Milk ◽  
Biochemical Analyses ◽  
Growth Assessment ◽  
Biochemical Assessment

Monitoring of Biochemical Parameters in Preterm Infants on Special Regime FeedingEnriched human milk may stimulate gain weight in preterm infants during the neonatal period. Aim is the biochemical assessment of preterm infants, fed by mother's milk fortificated with the special domestic formulapreImpamil during the first month of life. 80 preterm infants (45 male and 35 female), up to 36 weeks of gestation, BW less than 2500 g, started enteral intake in the first three days of life. Total volume intake was in range from 70 mL/kg first day, to 170-200 mL/kg after a 10thday of life. Mother's milk fortification was prepared as a 5% mixture solution ofpreImpamil. The dynamics of biochemical analyses started on the first day of study and was repeated once a week. We analysed levels of: total protein, albumin, prealbumin, transferin, urea, Ca ionised, P and alkaline phosphatase using standard biochemical methods. Statistical analyses were completed by ANOVA test, one-factorial analyses of variance. During monitoring the total protein level increased in the second week of life (p<0.01), as well as albumin (p<0.01). Prealbumin level increased, as well as transferin (p-NS). Initial level of ionised fraction of Ca was significantly lower (p<0.01) at the beginning of the study, compared to the rest. Serum level of P increased, as well as the level of alkaline phosphatase at the end of first and second week (p<0.01). Conclusion is that biochemical parameters, such as levels of protein and albumin, are important in the growth assessment of preterm infants on special feeding regimes.

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