scholarly journals Effect of Genetically Modified Feeds on Physico-Chemical Properties of Pork

2011 ◽  
Vol 11 (4) ◽  
pp. 597-606 ◽  
Author(s):  
Joanna Stadnik ◽  
Małgorzata Karwowska ◽  
Zbigniew Dolatowski ◽  
Małgorzata Świątkiewicz ◽  
Krzysztof Kwiatek
Keyword(s):  
Soybean Meal ◽  
Genetically Modified ◽  
Chemical Properties ◽  
Neck Muscles ◽  
Transgenic Maize ◽  
Gm Maize ◽  
Group I ◽  
Physico Chemical ◽  
Group Ii ◽  
Gm Soybean

Effect of Genetically Modified Feeds on Physico-Chemical Properties of PorkThe objective of this study was to evaluate the effects of genetically modified (GM), insect-resistant Bt maize (MON810) and the meal made of glyphosate-tolerant soybean (Roundup Ready MON40-3-2) used as the dietary components for pigs on the physico-chemical properties of meat. Forty-eight fatteners derived from Polish Landrace x Polish Large White sows mated to a Duroc x Pietrain boar were used. All animals received isonitrogenous and isoenergetic diets containing or not containing the genetically modified components. The design of the experiment was as follows: group I (control) - non-modified soybean meal and maize; group II - GM soybean meal and non-modified maize; group III - non-modified soybean meal and GM maize; group IV - GM soybean meal and GM maize. The examination of the pH values of loin and neck muscles indicated no statistically significant differences between pigs fed diets containing non-transgenic or transgenic feeds. No statistical differences were observed for water holding capacity (WHC) within dietary treatments. The introduction of transgenic maize and soybean meal into pig diets did not significantly affect the a* colour parameter of loin as well as neck muscles. The use of transgenic maize or soybean meal did not cause significant changes in the L* colour value of loin. Results obtained for neck muscles were more differentiated, possibly due to the natural heterogeneity of this primal cut. Pigs which had consumed the transgenic diet exhibited slightly decreased lipid stability of loin, as indicated by thiobarbituric acid reactive substances (TBARS). The decrease was statistically significant only in the case of muscles from group II. The addition of feeds derived from genetically modified crops into pig diets did not significantly affect the stability of neck muscle lipids; however, TBARS values of these muscles were twice those of loin muscles.

scholarly journals Effect of a Diet Composed of Genetically Modified Feed Components on the Selected Immune Parameters in Pigs, Cattle, and Poultry

2013 ◽  
Vol 57 (2) ◽  
pp. 209-217 ◽  
Author(s):  
Dariusz Bednarek ◽  
Katarzyna Dudek ◽  
Krzysztof Kwiatek ◽  
Małgorzata Świątkiewicz ◽  
Sylwester Świątkiewicz ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Genetically Modified ◽  
Group Iv ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Immune Parameters ◽  
Group I ◽  
Toxin Protein ◽  
Gm Soybean

Abstract The aim of the study was to evaluate the immune effects of genetically modified (GM), insect resistant corn (MON810) expressing toxin protein of Bacillus thuringiensis, and glyphosate-tolerant soybean meal (Roundup Ready MON-40-30-2), which are used as the feed mixture components in domestic animals. The study was conducted on 60 pigs (36 fatteners and 24 sows), 20 calves, 40 broilers, and 40 laying hens. Each species was divided into four basic nutritional groups: group I (control) - conventional feed, group II - feed consisted of GM soybean meal and non-modified corn, group III - non-modified soybean meal and GM corn, group IV - GM soybean meal and GM corn. Moreover, in the experiment on fatteners two additional groups were formed: group V - animals fed both conventional soybean meal and bruised grain, and group VI - GM soybean meal and conventional bruised grain. The results of study did not reveal any significant effect of feed mixtures containing GM components on the immune response in all animals regardless of their species and technological producing groups.

scholarly journals Effect of feeding genetically modified maize and soybean meal to sows on their reproductive traits, haematological indices and offspring performance

2013 ◽  
Vol 57 (3) ◽  
pp. 413-418 ◽  
Author(s):  
Małgorzata Świątkiewicz ◽  
Dariusz Bednarek ◽  
Jan Markowski ◽  
Ewa Hanczakowska ◽  
Krzysztof Kwiate
Keyword(s):  
Soybean Meal ◽  
Genetically Modified ◽  
Genetically Modified Maize ◽  
Bt Maize ◽  
Offspring Performance ◽  
Gm Maize ◽  
Lactating Sows ◽  
Maize Mon810 ◽  
Haematological Indices ◽  
Gm Soybean

Abstract The effect of Roundup Ready MON-40-3-2 soybean meal and Bt maize MON810 on sows performance and haematological indices, including parameters of erythrocytes, leukocytes, and trombocytes, as well as the piglets rearing indices were evaluated in the experiment carried out on 24 sows and their progeny. After mating sows were divided into groups: I - control, conventional soybean meal and conventional maize; II - genetically modified (GM) soybean meal and conventional maize; III - conventional soybean meal and GM maize; IV - GM soybean meal and GM maize. All used feed mixtures were isonitrogenous and isoenergetic, however differed in presence or absence of genetically modified maize MON810 (5% for pregnant and 8% for lactating sows) and/or soybean meal MON-40-3-2 (4% for pregnant and 14% for lactating sows). Born piglets were allotted to the same group as their mothers. The study showed that feeding pregnant and lactating sows with mixtures containing genetically modified RR soybean or/and meal Bt maize did not significantly affect their reproductive characteristics and offspring performance. There was no effect of dietary treatment on haematological indices. Transfer of transgenic DNA from GM feed to blood was not confirmed.

scholarly journals Physico-Chemical Properties of Soybean Meal-Based Adhesives Reinforced by Ethylene Glycol Diglycidyl Ether and Modified Nanocrystalline Cellulose

Polymers ◽  
2017 ◽  
Vol 9 (12) ◽  
pp. 463 ◽  
Author(s):  
Xiaona Li ◽  
Mingsong Chen ◽  
Jizhi Zhang ◽  
Qiang Gao ◽  
Shifeng Zhang ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Soybean Meal ◽  
Chemical Properties ◽  
Diglycidyl Ether ◽  
Nanocrystalline Cellulose ◽  
Physico Chemical

scholarly journals Effect of different fat supplements used during dry period of cows on colostrum physicochemical properties

2007 ◽  
Vol 23 (5-6-2) ◽  
pp. 67-75
Author(s):  
A. Zachwieja ◽  
T. Szulc ◽  
A. Potkański ◽  
R. Mikuła ◽  
W. Kruszyński ◽  
...  
Keyword(s):  
Rapeseed Oil ◽  
Chemical Properties ◽  
Feed Additives ◽  
Feed Additive ◽  
Dry Period ◽  
Group I ◽  
Physico Chemical ◽  
Number Of Microorganisms ◽  
Fat Supplements ◽  
Thermo Stability

The results of certain studies indicate that a relation exists between the quality of colostrum and milk and the correct balancing of energy and protein in the diet for cows in particular during the last three weeks before parturition. The aim of this study was to determine the effect of fat additives offered to cows during the dry period on the composition and physico-chemical properties of colostrum and the pre-colostrum secretion. 24 cows were assigned to one of three groups. Group I - control, received no feed additives; II - received feed supplemented by a mixture of fish and rapeseed oil in a 1:1 ratio, III - received feed supplemented by protected fat (Brgafat). In both cases the addition of fat amounted to 360g, i.e. 2% DM. All animals received a PMR concentrate in quantities calculated according to the INRA system. From all the cows samples were taken about 48 hours before parturition of the pre-colostrum secretion and directly after calving of colostrum from the first, complete milking. The samples taken were analyzed for basic composition, for the overall number of microorganisms (ONM), somatic cell count (SSC), content of urea, coagulation time after adding rennet, thermo stability as well as potential (oSH) and active (pH) acidity. The results of the studies conducted indicate that the composition and physico-chemical properties of colostrum and pre-colostrum is differentiated. Offering protected fat as a feed additive for cows during the last three weeks of the dry period had a significant effect on the share of dry matter and crude protein in the colostrum produced. The addition of a mixture of fish and rapeseed oil did not have a similar effect.

Decoration of specific sites on freeze-fractured membranes

1978 ◽  
Vol 36 (2) ◽  
pp. 140-141
Author(s):  
H. Gross ◽  
H. Moor
Keyword(s):  
Pure Water ◽  
Freeze Fracture ◽  
Chemical Properties ◽  
Surface Forces ◽  
Sulfate Pentahydrate ◽  
Copper Sulfate Pentahydrate ◽  
Physico Chemical ◽  
Water Of Hydration ◽  
Small Container ◽  
Binding Forces

Fracturing under ultrahigh vacuum (UHV, p ≤ 10-9 Torr) produces membrane fracture faces devoid of contamination. Such clean surfaces are a prerequisite foe studies of interactions between condensing molecules is possible and surface forces are unequally distributed, the condensate will accumulate at places with high binding forces; crystallites will arise which may be useful a probes for surface sites with specific physico-chemical properties. Specific “decoration” with crystallites can be achieved nby exposing membrane fracture faces to water vopour. A device was developed which enables the production of pure water vapour and the controlled variation of its partial pressure in an UHV freeze-fracture apparatus (Fig.1a). Under vaccum (≤ 10-3 Torr), small container filled with copper-sulfate-pentahydrate is heated with a heating coil, with the temperature controlled by means of a thermocouple. The water of hydration thereby released enters a storage vessel.

Ultrastructural Lesions Produced by Alcohol and Sucrose in the Heart and Liver of C57BL Mice

1970 ◽  
Vol 28 ◽  
pp. 208-209
Author(s):  
K.K. SEKHRI ◽  
C.S. ALEXANDER ◽  
H.T. NAGASAWA
Keyword(s):  
Osmium Tetroxide ◽  
Male Mice ◽  
Alcohol Group ◽  
Group Iii ◽  
Group I ◽  
C57bl Mice ◽  
Group Ii

C57BL male mice (Jackson Lab., Bar Harbor, Maine) weighing about 18 gms were randomly divided into three groups: group I was fed sweetened liquid alcohol diet (modified Schenkl) in which 36% of the calories were derived from alcohol; group II was maintained on a similar diet but alcohol was isocalorically substituted by sucrose; group III was fed regular mouse chow ad lib for five months. Liver and heart tissues were fixed in 2.5% cacodylate buffered glutaraldehyde, post-fixed in 2% osmium tetroxide and embedded in Epon-araldite.

Heterogeneity and Immunochemical Properties of Anti-β2-glycoprotein I Autoantibodies

1998 ◽  
Vol 80 (09) ◽  
pp. 393-398 ◽  
Author(s):  
V. Regnault ◽  
E. Hachulla ◽  
L. Darnige ◽  
B. Roussel ◽  
J. C. Bensa ◽  
...  
Keyword(s):  
Fluid Phase ◽  
Anticardiolipin Antibodies ◽  
Dual Reactivity ◽  
Group Iii ◽  
Important Group ◽  
Epitope Specificity ◽  
Glycoprotein I ◽  
Group I ◽  
Group Ii ◽  
Sufficient Concentration

SummaryMost anticardiolipin antibodies (ACA) associated with antiphospholipid syndrome (APS) are directed against epitopes expressed on β2-glycoprotein I (β2GPI). Despite a good correlation between standard ACA assays and those using purified human β2GPI as the sole antigen, some sera from APS patients only react in the latter. This is indicative of heterogeneity in anti-β2GPI antibodies. To characterize their reactivity profiles, human and bovine β2GPI were immobilized on γ-irradiated plates (β2GPI-ELISA), plain polystyrene precoated with increasing cardiolipin concentrations (CL/β2GPI-ELISA), and affinity columns. Fluid-phase inhibition experiments were also carried out with both proteins. Of 56 selected sera, restricted recognition of bovine or human β2GPI occurred respectively in 10/29 IgA-positive and 9/22 IgM-positive samples, and most of the latter (8/9) were missed by the standard ACA assay, as expected from a previous study. Based on species specificity and ACA results, IgG-positive samples (53/56) were categorized into three groups: antibodies reactive to bovine β2GPI only (group I) or to bovine and human β2GPI, group II being ACA-negative, and group III being ACA-positive. The most important group, group III (n = 33) was characterized by (i) binding when β2GPI was immobilized on γ-irradiated polystyrene or cardiolipin at sufficient concentration (regardless of β2GPI density, as assessed using 125I-β2GPI); (ii) and low avidity binding to fluid-phase β2GPI (Kd in the range 10–5 M). In contrast, all six group II samples showed (i) ability to bind human and bovine β2GPI immobilized on non-irradiated plates; (ii) concentration-dependent blockade of binding by cardiolipin, suggesting epitope location in the vicinity of the phospholipid binding site on native β2GPI; (iii) and relative avidities approximately 100-fold higher than in group III. Group I patients were heterogeneous with respect to CL/β2GPI-ELISA and ACA results (6/14 scored negative), possibly reflecting antibody differences in terms of avidity and epitope specificity. Affinity fractionation of 23 sera showed the existence, in individual patients, of various combinations of antibody subsets solely reactive to human or bovine β2GPI, together with cross-species reactive subsets present in all samples with dual reactivity namely groups III and II, although the latter antibodies were poorly purified on either column. Therefore, the mode of presentation of β2GPI greatly influences its recognition by anti-β2GPI antibodies with marked inter-individual heterogeneity, in relation to ACA quantitation and, possibly, disease presentation and pathogenesis.

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