PERSILANGAN INTERSPESIFIK ANGGREK HITAM (Coelogyne pandurata) DENGAN ANGGREK MUTIARA (Coelogyne asperata)

Black orchid (Coelogyne pandurata) and Pearl orchid (Coelogyne asperata) are endemic orchid from Kalimantan forest, shape and color suitable as ornamental flower. Many people collected the orchids from the wild for commercial purpose but not much effort on their propagation. To prevent population decreased, propagation and production of new varities via interspesific hybridisation need to be done. An important step required in plant breeding is to obtain cross compatiblity. Cross compatibility of some orchids from Indonesia are not known, including compatibility of Coelogyne pandurata x Coelogyne asperata. Information on compatibility and fertility is very important to produce good quality seed as propagation material. In this research pollination was performed on orchid plants at 2 until 5 day after flowering and pollination was done in the morning. There are three replicates for each pollination. Pollination was done with C. pandurata (black orchid) as male parent and C. asperata (pearl orchid) as female parent. The results showed that successful pollination was obtained from pollination at 2 days after flowering. Seed capsule was harvested at 3 months after pollination but not yet perfectly ripe. Seed germination employed in vitro method on Vacin & Went (VW) and VW with modification media. Seed from C. pandurata x C. asperata abled to germinate on VW media with addition of 0,1 mg/l auxin and 0.1 mg/l cytokinin and germinated 9 weeks after planting.

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A in Vivo Assay for Standardizing Heparin Preparations

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646810 ◽

1979 ◽

Vol 41 (03) ◽

pp. 576-582

Author(s):

A R Pomeroy

Keyword(s):

In Vitro Assays ◽

British Pharmacopoeia ◽

In Vitro Method ◽

Standard Preparation ◽

Reliable Estimation ◽

Assay Procedure ◽

Accuracy And Precision ◽

Heparin Preparation

SummaryThe limitations of currently used in vitro assays of heparin have demonstrated the need for an in vivo method suitable for routine use.The in vivo method which is described in this paper uses, for each heparin preparation, four groups of five mice which are injected intravenously with heparin according to a “2 and 2 dose assay” procedure. The method is relatively rapid, requiring 3 to 4 hours to test five heparin preparations against a standard preparation of heparin. Levels of accuracy and precision acceptable for the requirements of the British Pharmacopoeia are obtained by combining the results of 3 to 4 assays of a heparin preparation.The similarity of results obtained the in vivo method and the in vitro method of the British Pharmacopoeia for heparin preparations of lung and mucosal origin validates this in vivo method and, conversely, demonstrates that the in vitro method of the British Pharmacopoeia gives a reliable estimation of the in vivo activity of heparin.

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Standardization of protocol for in vitro seed germination of Citrus indica Tanaka

Crop Research ◽

10.31830/2454-1761.2020.021 ◽

2020 ◽

Vol 55 (3&4) ◽

Keyword(s):

Seed Germination

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Ophthalmic optics. Contact lenses. Ageing by exposure to UV and visible radiation (in vitro method)

10.3403/01319030 ◽

1998 ◽

Keyword(s):

Contact Lenses ◽

Visible Radiation ◽

In Vitro Method

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Seed-borne fungi of cowpea [Vigna unguiculata (L.) Walp] and their possible control in vitro using locally available fungicides in Botswana.

International Journal of Bioassays ◽

10.21746/ijbio.2016.11.005 ◽

2016 ◽

Vol 5 (11) ◽

pp. 5016 ◽

Cited By ~ 1

Author(s):

K. B. Khare* ◽

Loeto D. ◽

Wale K. ◽

Salani M.

Keyword(s):

Seed Germination ◽

Continuous Light ◽

Sodium Hypochlorite Solution ◽

Distilled Water ◽

Percentage Germination ◽

Department Of Agriculture ◽

Hypochlorite Solution ◽

Cowpea Cultivars ◽

Petri Plate

Seeds of three cowpea cultivars namely Black eye, ER 7 and Tswana obtained from the Department of Agriculture Research, Gaborone were tested for the presence of seed-borne fungi, and their possible control in vitro using locally available fungicides. Four hundred fifty seeds of each cultivar of cowpea were disinfected with 2% sodium hypochlorite solution for 10 min and washed three times with sterile distilled water before placing them in PDA plates (5 seeds/9 cm Petri plate), incubated at 22±2o C for 12 hour each under continuous light and dark. A total of eight fungi were detected from seeds of cowpea. These were Aspergillus flavus, A. niger, Cylindrocarpon sp., Fusarium equisiti, F. oxyaporum, Penicillium chyrosogenum, Rhizopus oligosporus and R. stolonifer. Rhizopus spp. were dominant fungi recovered from seeds, followed by Penicillium, Aspergillus, Fusarium and Cylindrocarpon. The fungi detected resulted in decay and rotting of seeds, and thereby reducing percentage germination of seeds (22%, 37% and 63 % seed germination in Black eye, ER7 and Tswana varieties of cowpea respectively). Out of four fungicides tested, benlate, captan, dithane M 45 and chlorothalanil. Dithane M45 effectively controlled seed-borne fungi, and enhanced seed germination to an average of 86% (93% germination with no fungi detected in Tswana variety) as compared to chlorothalonile (79%), benlate and captan (77%) and un-treated seeds (45%). The fungal incidence was reduced to 2.3%, 4.3%, 5.3% and 5.3% when seeds were treated with dithane M-45, chlorothalonil, benlate and captan respectively as compared to 62% in non-treated seeds.

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In vitro method for teaching percutaneous vascular catheterization.

American Journal of Roentgenology ◽

10.2214/ajr.157.5.1927800 ◽

1991 ◽

Vol 157 (5) ◽

pp. 1125-1125

Author(s):

D J Eschelman ◽

A J Greenfield ◽

D T Gibbens

Keyword(s):

In Vitro Method ◽

Vascular Catheterization

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Phytase-Producing Rahnella aquatilis JZ-GX1 Promotes Seed Germination and Growth in Corn (Zea mays L.)

Microorganisms ◽

10.3390/microorganisms9081647 ◽

2021 ◽

Vol 9 (8) ◽

pp. 1647

Author(s):

Gui-E Li ◽

Wei-Liang Kong ◽

Xiao-Qin Wu ◽

Shi-Bo Ma

Keyword(s):

Seed Germination ◽

Plant Growth ◽

Biomass Accumulation ◽

Root Surface ◽

Phosphorus Concentration ◽

Maize Seedlings ◽

Total Phosphorus Concentration ◽

Rahnella Aquatilis ◽

Maize Seeds

Phytase plays an important role in crop seed germination and plant growth. In order to fully understand the plant growth-promoting mechanism by Rahnella aquatilis JZ-GX1，the effect of this strain on germination of maize seeds was determined in vitro, and the colonization of maize root by R. aquatilis JZ-GX1 was observed by scanning electron microscope. Different inoculum concentrations and Phytate-related soil properties were applied to investigate the effect of R. aquatilis JZ-GX1 on the growth of maize seedlings. The results showed that R. aquatilis JZ-GX1 could effectively secrete indole acetic acid and had significantly promoted seed germination and root length of maize. A large number of R. aquatilis JZ-GX1 cells colonized on the root surface, root hair and the root interior of maize. When the inoculation concentration was 107 cfu/mL and the insoluble organophosphorus compound phytate existed in the soil, the net photosynthetic rate, chlorophyll content, phytase activity secreted by roots, total phosphorus concentration and biomass accumulation of maize seedlings were the highest. In contrast, no significant effect of inoculation was found when the total P content was low or when inorganic P was sufficient in the soil. R. aquatilis JZ-GX1 promotes the growth of maize directly by secreting IAA and indirectly by secreting phytase. This work provides beneficial information for the development and application of R. aquatilis JZ-GX1 as a microbial fertilizer in the future.

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