scholarly journals Comparative analysis of the results of bacteriological and chromato-mass-spectrometric studies of pleural exudate in patients with pleural empyema

2020 ◽  
Vol 179 (3) ◽  
pp. 40-47
Author(s):  
B. N. Kotiv ◽  
I. I. Dzidzava ◽  
T. N. Suborova ◽  
G. V. Valiev ◽  
O. V. Barinov ◽  
...  
Keyword(s):  
Pleural Cavity ◽  
Pleural Empyema ◽  
Mass Spectrometric ◽  
Diagnostic Methods ◽  
Gram Negative Bacteria ◽  
Bacteriological Examination ◽  
Gram Negative ◽  
Anaerobic Microorganisms ◽  
Causal Treatment ◽  
Pleural Exudate

The OBJECTIVE was to determine the possibility of using chromatography-mass spectrometry to select causal treatment for patients with pleural empyema.METHODS AND MATERIALS. The analysis of the results of examination and treatment of 207 patients with pleural empyema for the period from 2017 to 2018 was done. All patients underwent bacteriological examination, twenty patients from them underwent chromato-mass-spectrometric examination of pleural exudate.RESULTS. Patients were transferred to a specialized thoracic hospital from other medical institutions, where they received a course of empirical antibacterial therapy and drainage of the pleural cavity was performed. Bacteriological examination of the contents of the pleural cavity was positive in 112 (54.1 %) patients. The leading pathogens were gram-negative bacteria isolated from the contents of the pleural cavity in 45 % of patients with closed and 63.5 % – open pleural empyema. Polyantibiotic-resistant stocks of P. aeruginosa (30.4 %), K. pneumoniae (19.6 %) and A. baumannii (12.5 %) prevailed, which remained sensitive to polymyxin and, in some cases, to amikacin. In 25 (22.3 %) patients, micromycetes of the genus Candida were found. No growth of anaerobic microflora was detected. Chromato-mass-spectrometric examination of pleural exudate revealed markers of 30 taxa of bacteria, viruses and fungi that exceeded the norm by more than two times. Markers of gram-negative bacteria were not detected. The proportion of anaerobic microorganisms was 76.6 %, with the highest concentration found for bacteria of the genus Clostridium and Eubacterium.CONCLUSION. The choice of causal treatment for patients with pleural empyema is difficult due to the negative culturing from the contents of the pleural cavity in 45.9 % of patients, as well as the discordance between the results of bacteriological and chromato-mass-spectrometric studies. Diagnostic methods for detecting pathogens of pleural empyema require further improvement.

APPLICATION OF PLEURAL EFFUSION CRYSTALLOGRAPHY IN THE DIAGNOSIS OF CHRONIC PLEURAL EMPYEMA

2020 ◽  
Vol 5 (8(77)) ◽  
pp. 5-9
Author(s):  
S.A. Kalashnikova ◽  
S.Sh. Aidaeva ◽  
E.A. Kalashnikova
Keyword(s):  
Experimental Study ◽  
Pleural Effusion ◽  
Morphometric Analysis ◽  
Spatial Organization ◽  
Pleural Cavity ◽  
Biological Fluid ◽  
Pleural Empyema ◽  
Pleural Exudate

An experimental study of facies of pleural exudate obtained from the residual pleural cavity of rats with chronic pleural empyema at different periods of the experiment was conducted. As a result of morphometric analysis of exudate facies, a violation of the structural and spatial organization of the biological fluid and the etiology of pleural effusion was established.

scholarly journals Treatment of Newborns and Infants in Japan suffering from Pneumonia with New Antibiotics

2017 ◽  
Vol 15 (3-4) ◽  
pp. 105
Author(s):  
Susumu Nakazawa
Keyword(s):  
Combination Therapy ◽  
Mixed Infection ◽  
Chemotherapeutic Agents ◽  
Gram Negative Bacteria ◽  
Bacteriological Examination ◽  
High State ◽  
Bactericidal Action ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
New Antibiotics

Despite the recent development of a high state of chemotherapy in Japan, there has not necessarily been an improvement in the prognosis of pneumonia in infants and newborns. Upon bacteriological examination, relevant causative organisms such as pathogenic staphylococci, pneumococci, streptococci, and gram-negative bacteria have been detected as causing mixed infection. Suitable chemotherapeutic agents which diffuse well to the lung and show high bactericidal action against such organisms must be selected to combat the infection. A combination of two synthetic penicillins, ampicillin, effective against gram-negative as well as gram-positive bacteria and cloxacillin effective against resistant staphylococci, was used for the treatment of many cases of pneumonia in infants and newborns and satisfactory results were obtained. The results of this study and an appraisal of penicillin combination therapy will be discussed.

scholarly journals Volatile Constituents of the Leaves of Aniba hostmanniana (Lauraceae) and their Antibacterial Activities

2015 ◽  
Vol 10 (7) ◽  
pp. 1934578X1501000
Author(s):  
Wilberto De Lima ◽  
Luis B. Rojas-Fermín ◽  
Sonia Koteich-Khatib ◽  
María Eugenia Lucena ◽  
Juan Carmona Arzola
Keyword(s):  
Escherichia Coli ◽  
Essential Oil ◽  
Antibacterial Activities ◽  
Mass Spectrometric ◽  
Klebsiella Pneumonia ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Positive Bacterium ◽  
Benzyl Benzoate ◽  
Gram Negative

The essential oil of Aniba hostmanniana (Nees) Mez, family Lauraceae, was obtained from fresh leaves by hydrodistillation. The chemical composition of the oil was assessed using gas chromatography with mass spectrometric detection (GC-MS). The antimicrobial activity of the essential oil was tested against Gram-negative bacteria ( Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia) and the Gram-positive bacterium Staphylococcus aureus. GC-MS analysis revealed the presence of 35 compounds in the essential oil, the predominant ones being benzyl benzoate (29.3%), δ-cadinene (12%), β-caryophyllene (10.5%), bicyclogermacrene (5.9%), and α-copaene (3.9%). The oil showed activity against most of the bacterial strains studied.

scholarly journals MICROBIOLOGICAL DIAGNOSTICS OF PYOINFLAMMATORY ABDOMINAL DISEASES

2016 ◽  
Vol 1 (4) ◽  
pp. 95-98
Author(s):  
Аюшинова ◽  
Natalya Ayushinova ◽  
Владимирова ◽  
Lyudmila Vladimirova ◽  
Ветохина ◽  
...  
Keyword(s):  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Anaerobic Microorganisms ◽  
Microbiological Diagnostics ◽  
Wall Specimen ◽  
Clinically Significant ◽  
Optimal Material ◽  
Aerobic And Anaerobic ◽  
Positive Results

The aim of the work was to establish the significance of complex bacteriological research in the diagnostics of acute appendicitis and to determine an optimal material for the research based on the findings. We examined 19patients with acute phlegmonous or acute gangrenous appendicitis (males, aged 18–60years). We performed bacteriological research of abdominal exudate (n=19) and biopsy specimen (n=19) of appendix wall taken before opening the lumen of the intestine. Both abdominal exudate and appendix wall specimen were taken at the same time. Aerobic and anaerobic microorganisms were detected and identified, antimicrobial susceptibility was tested. In total, we detected 25 strains of aerobic and 13 strains of anaerobic microorganisms. It has been established that a bioptate was most informative for testing (68.4 %); the parallel study of an abdominal exudate gave positive results in 21.1 % of cases. In the structure of clinically significant microflora dominated E.coli (43.3%), then went nonfermentative gram-negative bacteria (13.3%) and Bacteroidesspp. (16.7%). We marked growing resistance of detected strains of gram-negative bacteria to some antibiotics. For instance, 62 % of detected E.c oli strains were resistant to ampicillin, 25 % – to ciprofloxacin. 92 % of strains were resistant to cefepime, 93 % – to ceftriaxone, 77 % – to Amoxiclav, 67 % – to gentamicin, 90 % – to tobramycin. From one bioptate a strain of E. coli ESBL was separated. The study of intraoperative bioptate of appendix wall increases effectiveness of microbiological diagnostics in com-parison with the abdominal exudate research.

Bacterial-Mucosal Cell Junctional Complexes

1974 ◽  
Vol 32 ◽  
pp. 144-145
Author(s):  
Roger C. Wagner
Keyword(s):  
Intestinal Wall ◽  
Rat Colon ◽  
Mucosal Cell ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Mucosal Cells ◽  
Mucosal Lining ◽  
Degenerative Alterations ◽  
Junctional Complexes ◽  
Intestinal Mucosal Cells

Bacteria exhibit the ability to adhere to the apical surfaces of intestinal mucosal cells. These attachments either precede invasion of the intestinal wall by the bacteria with accompanying inflammation and degeneration of the mucosa or represent permanent anchoring sites where the bacteria never totally penetrate the mucosal cells.Endemic gram negative bacteria were found attached to the surface of mucosal cells lining the walls of crypts in the rat colon. The bacteria did not intrude deeper than 0.5 urn into the mucosal cells and no degenerative alterations were detectable in the mucosal lining.

Rapid demonstration of septic or infectious arthritis due to Gram-negative bacteria

1992 ◽  
Vol 50 (1) ◽  
pp. 686-687
Author(s):  
Jacob S. Hanker ◽  
Paul R. Gross ◽  
Beverly L. Giammara
Keyword(s):  
Chronic Arthritis ◽  
Blood Cultures ◽  
Gram Negative Bacteria ◽  
Infectious Arthritis ◽  
Bacterial Arthritis ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Blood cultures are positive in approximately only 50 per cent of the patients with nongonococcal bacterial infectious arthritis and about 20 per cent of those with gonococcal arthritis. But the concept that gram-negative bacteria could be involved even in chronic arthritis is well-supported. Gram stains are more definitive in staphylococcal arthritis caused by gram-positive bacteria than in bacterial arthritis due to gram-negative bacteria. In the latter situation where gram-negative bacilli are the problem, Gram stains are helpful for 50% of the patients; they are only helpful for 25% of the patients, however, where gram-negative gonococci are the problem. In arthritis due to gram-positive Staphylococci. Gramstained smears are positive for 75% of the patients.

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