scholarly journals Research of the peculiarities of colloidal quantum dots of AgInS2/ZnS and chitosan nanoparticles labeled with indocyanine green as the fluorescent labels for biomedical applications

2018 ◽  
Vol 17 (1) ◽  
pp. 74-82 ◽  
Author(s):  
M. S. Istomina ◽  
E. I. Pochkaeva ◽  
D. L. Sonin ◽  
N. A. Pechnikova ◽  
V. N. Postnov ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Indocyanine Green ◽  
Fluorescence Imaging ◽  
Biomedical Applications ◽  
Chitosan Nanoparticles ◽  
Colloidal Quantum Dots ◽  
Fluorescent Labels ◽  
In Vitro Diagnostics

The research is devoted to the investigating of the possibility of using colloidal quantum dots, covered by various layers and nanoparticles of chitosan labelled with indocyanin green for fluorescence imaging in vitro and in vivo. Pure quantum dots and dots in the shell synthesized from 3-aminopropyltriethoxysilane and in the shell of albumin were investigated. Research using fluorescence Visualizer IVIS Lumina LT Series III (Perkin Elmer, USA) was conducted. The possibility of using quantum dots for in vitro diagnostics, and chitosan nanoparticles - in vitro and in vivo was presented.

scholarly journals Ultrasmall Quantum Dots: A Tool for in Vitro and in Vivo Fluorescence Imaging

2017 ◽  
Vol 784 ◽  
pp. 012033
Author(s):  
Pavel Linkov ◽  
Kirill V Vokhmintcev ◽  
Pavel S Samokhvalov ◽  
Igor Nabiev
Keyword(s):  
Quantum Dots ◽  
Fluorescence Imaging ◽  
In Vivo Fluorescence ◽  
In Vivo Fluorescence Imaging

One-Pot Synthesized Aptamer-Functionalized CdTe:Zn2+Quantum Dots for Tumor-Targeted Fluorescence Imaging in Vitro and in Vivo

2013 ◽  
Vol 85 (12) ◽  
pp. 5843-5849 ◽  
Author(s):  
Cuiling Zhang ◽  
Xinghu Ji ◽  
Yuan Zhang ◽  
Guohua Zhou ◽  
Xianliang Ke ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Fluorescence Imaging ◽  
One Pot

Exploring Feasibility of Multicolored CdTe Quantum Dots for In Vitro and In Vivo Fluorescent Imaging

2008 ◽  
Vol 8 (3) ◽  
pp. 1174-1177 ◽  
Author(s):  
Junfeng Su ◽  
Jun Zhang ◽  
Li Liu ◽  
Yalou Huang ◽  
Ralph P. Mason
Keyword(s):  
Quantum Dots ◽  
Fluorescence Imaging ◽  
Water Solubility ◽  
Imaging System ◽  
Fluorescent Imaging ◽  
Cdte Quantum Dots ◽  
Cdte Qds ◽  
Fluorescence Imaging System

We report the use of novel multicolored CdTe quantum dots (QDs) as fluorophores for biological fluorescence imaging. The CdTe QDs were prepared to exhibit emission wavelengths in the green, yellow, and red range by using trifluoroacetic acid (TFA), L-cysteine and thioglycolic acid (TGA) as surface stabilizers, respectively. The particles have good water solubility and photostability. Fluorescence imaging potential was evaluated in vitro and in vivo using a multispectral Maestro CRI Fluorescence Imaging system. The results show that different colored CdTe QDs allow sensitive detection simultaneously or separately both in vitro and in vivo against background fluorescence. The studies indicate that CdTe QDs can provide alternative fluorescent probes for biological imaging.

Probing Near-Infrared Quantum Dots for Imaging and Biomedical Applications

2011 ◽  
Vol 345 ◽  
pp. 3-11 ◽  
Author(s):  
Zi Hao Wang ◽  
Xue Feng Wang ◽  
Han Jiang ◽  
Jing Ding ◽  
Jian Dong Wang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Biomedical Applications ◽  
Near Infrared ◽  
Light Emission ◽  
Chemical Properties ◽  
Fluorescent Imaging ◽  
Light Emitting ◽  
Photo Stability

As light-emitting nanocrystals, quantum dots (QDs) have created a new realm of bioscience by combining nanomaterials with biology. They also have been a major focus of research and development during the past decade, which will profoundly influence future biological as well as biomedical research. In recent years, near-infrared (NIR) quantum dots have emerged in analytical applications, especially for in vitro and in vivo imaging. The impetus behind such endeavors can be attributed to their unique optical and chemical properties, with size-tunable light emission, high photo stability, and manifold fluorescence colors. In this review, we focus on fluorescent imaging with near-infrared (NIR) quantum dots (QDs) both in vitro and in vivo, and the advantages of QDs and potential problems to their use in practical biomedical applications. The ultimate targets aim at decreasing the cytotoxicity of QDs and the future outlook of QD applications in biomedical fields.

scholarly journals Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

2015 ◽  
Vol 44 (14) ◽  
pp. 4792-4834 ◽  
Author(s):  
K. David Wegner ◽  
Niko Hildebrandt
Keyword(s):  
Quantum Dots ◽  
Fluorescence Imaging ◽  
Semiconductor Quantum Dots ◽  
In Vivo Fluorescence ◽  
In Vivo Fluorescence Imaging

Colourful cells and tissues: semiconductor quantum dots and their versatile applications in multiplexed bioimaging research.

scholarly journals Dual-colour (near-infrared/visible) emitting annexin V for fluorescence imaging of tumour cell apoptosis in vitro and in vivo

RSC Advances ◽  
2020 ◽  
Vol 10 (63) ◽  
pp. 38244-38250
Author(s):  
Setsuko Tsuboi ◽  
Takashi Jin
Keyword(s):  
Tumor Cell ◽  
Indocyanine Green ◽  
Fluorescence Imaging ◽  
Cell Apoptosis ◽  
Near Infrared ◽  
Annexin V ◽  
Tumor Cell Apoptosis ◽  
Dual Colour

Indocyanine green labeled recombinant annexin V probes (ICG–EGFP–Annexin V and ICG–mPlum–Annexin V) were synthesized for near-infrared and visible fluorescence imaging of tumor cell apoptosis both in vitro and in vivo.

Biological and biomedical applications of collagenous biomaterials

1990 ◽  
Vol 48 (3) ◽  
pp. 856-857
Author(s):  
Yasushi P. Kato ◽  
Michael G. Dunn ◽  
Frederick H. Silver ◽  
Arthur J. Wasserman
Keyword(s):  
Biomedical Applications ◽  
Soft Tissues ◽  
Collagen Fibers ◽  
Bone Collagen ◽  
Cover Slip ◽  
Fibroblast Migration ◽  
Cellular Expression ◽  
Defect Repair

Collagenous biomaterials have been used for growing cells in vitro as well as for augmentation and replacement of hard and soft tissues. The substratum used for culturing cells is implicated in the modulation of phenotypic cellular expression, cellular orientation and adhesion. Collagen may have a strong influence on these cellular parameters when used as a substrate in vitro. Clinically, collagen has many applications to wound healing including, skin and bone substitution, tendon, ligament, and nerve replacement. In this report we demonstrate two uses of collagen. First as a fiber to support fibroblast growth in vitro, and second as a demineralized bone/collagen sponge for radial bone defect repair in vivo.For the in vitro study, collagen fibers were prepared as described previously. Primary rat tendon fibroblasts (1° RTF) were isolated and cultured for 5 days on 1 X 15 mm sterile cover slips. Six to seven collagen fibers, were glued parallel to each other onto a circular cover slip (D=18mm) and the 1 X 15mm cover slip populated with 1° RTF was placed at the center perpendicular to the collagen fibers. Fibroblast migration from the 1 x 15mm cover slip onto and along the collagen fibers was measured daily using a phase contrast microscope (Olympus CK-2) with a calibrated eyepiece. Migratory rates for fibroblasts were determined from 36 fibers over 4 days.

Conjugates of Classical DNA/RNA Binder with Nucleobase: Chemical, Biochemical and Biomedical Applications

2019 ◽  
Vol 26 (30) ◽  
pp. 5609-5624
Author(s):  
Dijana Saftić ◽  
Željka Ban ◽  
Josipa Matić ◽  
Lidija-Marija Tumirv ◽  
Ivo Piantanida
Keyword(s):  
Molecular Weight ◽  
Small Molecules ◽  
Biomedical Applications ◽  
Protein Targets ◽  
New Class ◽  
Rna Targets ◽  
Covalently Linked ◽  
Structural Aspects

: Among the most intensively studied classes of small molecules (molecular weight < 650) in biomedical research are small molecules that non-covalently bind to DNA/RNA, and another intensively studied class is nucleobase derivatives. Both classes have been intensively elaborated in many books and reviews. However, conjugates consisting of DNA/RNA binder covalently linked to nucleobase are much less studied and have not been reviewed in the last two decades. Therefore, this review summarized reports on the design of classical DNA/RNA binder – nucleobase conjugates, as well as data about their interactions with various DNA or RNA targets, and even in some cases protein targets are involved. According to these data, the most important structural aspects of selective or even specific recognition between small molecule and target are proposed, and where possible related biochemical and biomedical aspects were discussed. The general conclusion is that this, rather new class of molecules showed an amazing set of recognition tools for numerous DNA or RNA targets in the last two decades, as well as few intriguing in vitro and in vivo selectivities. Several lead research lines show promising advancements toward either novel, highly selective markers or bioactive, potentially druggable molecules.

scholarly journals Hexapod Assassins’ Potion: Venom Composition and Bioactivity from the Eurasian Assassin Bug Rhynocoris iracundus

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 819
Author(s):  
Nicolai Rügen ◽  
Timothy P. Jenkins ◽  
Natalie Wielsch ◽  
Heiko Vogel ◽  
Benjamin-Florian Hempel ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Galleria Mellonella ◽  
Systemic Reactions ◽  
Venom Composition ◽  
Assassin Bug ◽  
Molecular Components ◽  
First Time ◽  
Tissue Digestion

Assassin bug venoms are potent and exert diverse biological functions, making them potential biomedical goldmines. Besides feeding functions on arthropods, assassin bugs also use their venom for defense purposes causing localized and systemic reactions in vertebrates. However, assassin bug venoms remain poorly characterized. We collected the venom from the assassin bug Rhynocoris iracundus and investigated its composition and bioactivity in vitro and in vivo. It caused lysis of murine neuroblastoma, hepatoma cells, and healthy murine myoblasts. We demonstrated, for the first time, that assassin bug venom induces neurolysis and suggest that it counteracts paralysis locally via the destruction of neural networks, contributing to tissue digestion. Furthermore, the venom caused paralysis and melanization of Galleria mellonella larvae and pupae, whilst also possessing specific antibacterial activity against Escherichia coli, but not Listeria grayi and Pseudomonas aeruginosa. A combinatorial proteo-transcriptomic approach was performed to identify potential toxins responsible for the observed effects. We identified neurotoxic Ptu1, an inhibitory cystin knot (ICK) toxin homologous to ω-conotoxins from cone snails, cytolytic redulysins homologous to trialysins from hematophagous kissing bugs, and pore-forming hemolysins. Additionally, chitinases and kininogens were found and may be responsible for insecticidal and cytolytic activities. We demonstrate the multifunctionality and complexity of assassin bug venom, which renders its molecular components interesting for potential biomedical applications.

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