scholarly journals Antioxidant and Cytotoxic Potential of Local Endemic Plant Pastinaca zozimoides Fenzl

2021 ◽  
Vol 9 (4) ◽  
pp. 646-649
Author(s):  
Cihan Düşgün ◽  
Teoman Kankılıç ◽  
Cemil İşlek ◽  
Dilara Fatma Balı ◽  
Özgür Kankılıç
Keyword(s):  
Free Radical ◽  
Cell Lines ◽  
Total Phenolic Content ◽  
Cytotoxic Effect ◽  
Phenolic Content ◽  
Reducing Power ◽  
Total Phenolic ◽  
Cytotoxic Potential ◽  
Local Endemic ◽  
Reducing Power Assay

P. zozimoides is local endemic in Nigde province, Turkey. There has been no previous examination of the chemical composition and bioactivity of that plant extract. In this study, we studied total phenolic content, antioxidant properties and cytotoxic effect of methanolic extracts of plant sample. According to the Folin-Ciocalteau method, the total phenolics of the extracts were determined spectrophometric. The antioxidant activity was conducted DPPH (1,1’-diphenyl-2-picrylhydrazyl) radical scavenging method and free radical reducing power assay. The cytotoxic effect was studied using MTT assay cell viability on cancer cell lines as well as on Caco-2 cell lines. The total phenolic content of P. zozimoides extract was found 28.79±0.68 μg GAE /mg. The free radical scavenger activities of the DPPH was 31.69%±1.61, 85.15%±0.13, 86.96%±0.085 for 1, 5 and 10 mg/mL, respectively. The free radical reducing power assay was 0.375±0.52, 1.587±0.71, 1.798±0.84 for 1, 5 and 10 mg/mL compared to ascorbic acid standard. On Caco-2 cell lines, the extract of plant showed no cytotoxic potential. Because of its phenolic constituents and its antioxidant capacity it can be considered a healthy nutrient.

scholarly journals Total Phenolic Content, Free Radical Scavenging Activity and Reducing Power of Quisqualis indica Linn.

1970 ◽  
Vol 8 (2) ◽  
pp. 173-175 ◽  
Author(s):  
Md Abul Kaisar ◽  
Mohammad Rashedul Islam ◽  
Mohammad Sarifur Rahman ◽  
Md Khalid Hossain ◽  
Mohammad A Rashid
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity

DOI: 10.3329/dujps.v8i2.6034 Dhaka Univ. J. Pharm. Sci. 8(2): 173-175, 2009 (December)

scholarly journals New insights of phenolic compounds from optimized fruit extract of Ficus auriculata

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Shahinuzzaman ◽  
Parul Akhtar ◽  
N. Amin ◽  
Yunus Ahmed ◽  
Farah Hannan Anuar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Optimum Extraction

AbstractIn this study, the extraction conditions extracted maximize amounts of phenolic and bioactive compounds from the fruit extract of Ficus auriculata by using optimized response surface methodology. The antioxidant capacity was evaluated through the assay of radical scavenging ability on DPPH and ABTS as well as reducing power assays on total phenolic content (TPC). For the extraction purpose, the ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P < 0.0001) and total phenolic content (R2 = 0.961, P < 0.0001), indicating a significant correlation between the experimental and expected value. The highest DPPH radical scavenging activity was achieved 85.20 ± 0.96% at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC–ESI–MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.

scholarly journals Antioxidant Rich Extracts of Terminalia ferdinandiana Inhibit the Growth of Foodborne Bacteria

Foods ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 281 ◽  
Author(s):  
Saleha Akter ◽  
Michael E. Netzel ◽  
Ujang Tinggi ◽  
Simone A. Osborne ◽  
Mary T. Fletcher ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Food Preservation ◽  
Total Phenolic ◽  
Foodborne Bacteria ◽  
Terminalia Ferdinandiana

Terminalia ferdinandiana (Kakadu plum) is a native Australian plant containing phytochemicals with antioxidant capacity. In the search for alternatives to synthetic preservatives, antioxidants from plants and herbs are increasingly being investigated for the preservation of food. In this study, extracts were prepared from Terminalia ferdinandiana fruit, leaves, seedcoats, and bark using different solvents. Hydrolysable and condensed tannin contents in the extracts were determined, as well as antioxidant capacity, by measuring the total phenolic content (TPC) and free radical scavenging activity using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. Total phenolic content was higher in the fruits and barks with methanol extracts, containing the highest TPC, hydrolysable tannins, and DPPH-free radical scavenging capacity (12.2 ± 2.8 g/100 g dry weight (DW), 55 ± 2 mg/100 g DW, and 93% respectively). Saponins and condensed tannins were highest in bark extracts (7.0 ± 0.2 and 6.5 ± 0.7 g/100 g DW). The antimicrobial activity of extracts from fruit and leaves showed larger zones of inhibition, compared to seedcoats and barks, against the foodborne bacteria Listeria monocytogenes, Bacillus cereus, Methicillin resistant Staphylococcus aureus, and clinical isolates of Pseudomonas aeruginosa. The minimum inhibitory concentration and minimum bactericidal concentration in response to the different extracts ranged from 1.0 to 3.0 mg/mL. Scanning electron microscopy images of the treated bacteria showed morphological changes, leading to cell death. These results suggest that antioxidant rich extracts of Terminalia ferdinandiana fruits and leaves have potential applications as natural antimicrobials in food preservation.

Effect of 1-MCP on Bioavailability of Lingwu Long Jujube In Vitro Digestion

2012 ◽  
Vol 554-556 ◽  
pp. 1357-1360
Author(s):  
Zhong Li Jiang ◽  
Ai Li Wang ◽  
Xi Hong Li ◽  
Min Peng Zhu ◽  
Jun Wei Wang
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Control Samples

The present study investigated the effect of 1-MCP on bioavailability of Lingwu long jujube stored at 0 °C for 60 days. At the end of the storage, compared with the control samples, Lingwu long jujube treated with 1-MCP exhibited higher Vc bioaccessibility, total phenolic content and free radical scavenging capacity, which demonstrated that 1-MCP treatment could provide a better effect on maintaining the bioavailability of Lingwu long jujube.

scholarly journals Antioxidant, Cytotoxic Activities and Total Phenolic Content of Four Indonesian Medicinal Plants

2012 ◽  
Vol 2 (4) ◽  
Author(s):  
Waras Nurcholis ◽  
Bambang Pontjo Priosoeryanto ◽  
Edy Djauhari Purwakusumah ◽  
Takeshi Katayama ◽  
Toshisada Suzuki
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Ic50 Values ◽  
Ethanol Extracts

The crude ethanol extracts of four Indonesian medicinal plants namely Curcuma xanthorrhiza Roxb.,Phyllanthus niruri Linn., Andrographis paniculata Ness., and Curcuma aeruginosa Roxb. wereexamined for their antioxidant (radical scavenging) activity using 2, 2-diphenyl-2-picrylhydrazyl(DPPH) free radical and cytotoxicity using brine shrimp lethality test (BSLT). The total phenoliccontent was used the Folin-Ciocalteu method. IC50 values for DPPH radical scavenging activityranged from 14.5 to 178.5 μg/ml, with P. niruri having the lowest value and therefore the mostpotent, and C. aeruginosa having the highest value. LC50 values for BSLT ranged from 210.3 to593.2 μg/ml, with C. xanthorrhiza and A. paniculata having the lowest and highest values,respectively. The total phenolic content of the Indonesian plants ranged from 133.0 ±3.7 to863.3±54.7 mg tannic acid equivalent per 1 g extract, with C. aeruginosa and P. niruri having thelowest and highest values, respectively. A positive correlation between free radical scavengingactivity and the content of phenolic compounds was found in the four of Indonesian medicinal plants.

scholarly journals Evaluation of Total Phenolic Content in Relation to Antioxidant Activity of Brown Algae Cystoseira barbata from Black Sea

2019 ◽  
Vol 70 (7) ◽  
pp. 2684-2689
Author(s):  
Emin Cadar ◽  
Rodica Sirbu ◽  
Alef Ibram ◽  
Ana Maria Ionescu
Keyword(s):  
Antioxidant Activity ◽  
Benzoic Acid ◽  
Black Sea ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Reducing Power ◽  
The Black Sea ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Cystoseira Barbata

Seaweeds are widely used in the life science as source of compounds with diverse structural forms and biological activities, therefore, potential source of new antioxidant activity. The algae of the Black Sea have not been adequately explored for their potential as a source of bioactive substances. In this context, Brown algae Cystoseira barbata isolated from Black Sea coastal waters of Romania, were evaluated for their bioactivity potential. The results from Cystoseira barbata were obtained: for total phenolic content obtained in ethanolic extracts (TPC): 385.6 � 1.85 mg GAE/100 g f.w. Phenolic Acids quantified by HPLC-DAD from hydroethanolic extract of Cystoseira barbata from Black Sea was 80.14% from total phenolic content identified. We showed that Cystoseira barbata from the Romanian shore of the Black Sea contains vanillic acid in highest quantity (99.5�0.08 mg/100 g f.w.), followed by benzoic acid (65.7�0.06 mg/100 g f.w.) and feluric acid (54.5�0.01 mg/100 g f.w.). The smallest quantities were for gallic acid (3.5�0.03 mg/100 g f.w.), pyrogallol acid (4.2�0.05 mg/100 g f.w.) and 4-amino-benzoic acid (5.2�0.09 mg/100 g f.w.). Cystoseira barbata has a total content of large phenols and also has strong antioxidant and antimicrobial activity. Antioxidant activity (ACL) was studied using DPPH radical scavenging activity and reducing power. The extracts containing high levels of total phenolic content were also potent DPPH radical scavengers. The IC50 (�g/mL) for Cystoseira barbata in methanolic extract was 942.92� 2.56 �g/mL. High absorbance obtained from reducing power assay indicates high reducing power, measured values of absorbance varied from 0.0901 at 0.9223.

scholarly journals Pengaruh Penambahan Rempah dan Bentuk Minuman terhadap Aktivitas Antioksidan Berbagai Minuman Tradisional Indonesia

2017 ◽  
Vol 37 (1) ◽  
pp. 7 ◽  
Author(s):  
Aisyah Tri Septiana ◽  
Mohammad Samsi ◽  
Mustaufik Mustaufik
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Beverage Type ◽  
Radical Trapping ◽  
Palm Sugar

Indonesia traditional beverages such as minuman jahe, kunyit asam, beras kencur, and minuman temulawak are usually made with the addition of spices. They can be served in the form of  liquid or powder. This research was aimed to study the effect of  the beverage type, addition of spices, and the form of product on their antioxidant activity. The type of sugar used in this study was palm sugar juice. Total phenolic contents were measured to  indicate the level of antioxidants. Measurement of antioxidant  activity was conducted by analyzing malonaldehyde from linoleic  acid and capturing capacity of DPPH radical. The results showed  that Indonesian traditional beverage could inhibit the oxidation of linoleic acid and capture free radicals. Free radical capturing  capacity of curcuma beverage greater than other beverages, but  the malonaldehyde (MDA) inhibitor effect was not different. Total phenolic content of curcuma tended to be higher than the other traditional beverages. The addition of spices could raise the level  of total phenolic content in beverages and increase free radical  trapping capacity. Drying the beverages into powder could reduce the levels of total phenolic content and but did not reduce the  antioxidant activity. ABSTRAKMinuman tradisional Indonesia seperti minuman jahe, kunyit  asam, beras kencur, dan minuman temulawak biasanya dibuat  dengan tambahan berbagai rempah. Minuman tersebut dapat  disajikan dalam bentuk cair maupun serbuk. Penelitian ini  bertujuan untuk mengetahui pengaruh jenis minuman,  penambahan rempah tambahan, dan bentuk minuman terhadap aktivitas antioksidan minuman. Jenis gula yang ditambahkan  adalah gula nira. Jenis antioksidan yang diuji adalah total fenolik. Pengukuran aktivitas antioksidan dilakukan dengan uji  penghambatan malonaldehid dan kapasitas penangkapan radikal  DPPH. Hasil penelitian menunjukkan bahwa minuman  tradisional Indonesia dapat menghambat oksidasi asam linoleat dan menangkap radikal bebas. Kapasitas penangkapan radikal  bebas oleh minuman temulawak lebih besar dibandingkan  minuman yang lain tetapi kemampuan penghambatan  malonaldehid (MDA) oleh minumaan tradisional tidak berbeda.  Total fenolik minuman temulawak cenderung lebih besar  dibandingkan minuman tradisional yang lain. Penambahan  rempah-rempah dapat meningkatkan kadar total fenolik  minuman dan meningkatkan kapasitas penangkapan radikal  bebas. Pembuatan minuman serbuk pada penelitian ini dapat  menurunkan kadar total fenolik tetapi tidak menurunkan aktivitas antioksidan.

scholarly journals Antioxidant Activity and Total Phenolic Content in Shiitake Mycelial Exudates

2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Weihuan Huang ◽  
Joo-Shin Kim ◽  
Hau Yin Chung
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Shiitake Mushroom ◽  
Ferrous Ions

Exudates (DE) secreted from two shiitake mushroom mycelia (strains 1358 and L5458) were evaluated for their antioxidative properties and phenolic content. 1358DE and L5458DE showed distinct antioxidant activity in different in vitro assays, including scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, hydroxyl radical, superoxide anions and hydrogen peroxide; the ability to chelate ferrous ions; reducing power; hemolysis inhibition activity in rat erythrocyte; and lipid peroxidation inhibition (IC50 values of 1358DE and L5458DE were 3.3 and 132.6; 44.5 and > 1000; 26.9 and 53.7; 153.6 and >175.0; 176.0 and 521.0; 26.7 and 746.4; 47.8 and 736.9; and 3.1 and > 1000 μg/mL, respectively). Their total phenolic content was 237.33 and 24.08 mg gallic acid equivalent (GAE)/g of dry DE, respectively. Overall, these results show that 1358DE generally possesses better antioxidant properties than L5458DE, possibly due to its larger total phenolic content. Shiitake mushroom mycelial exudates, particularly of 1358DE, could be a good source of natural antioxidants.

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