scholarly journals Photodynamic effectiveness of laser diode combined with ozone to reduce STaphylicoccus aureus biofilm with exogenous chlorophyll of Dracaena angustifolia leaves

2019 ◽  
Vol 8 (2) ◽  
pp. 4-13 ◽  
Author(s):  
S. D. Astuti ◽  
N. D. Drantantiyas ◽  
A. P. Putra ◽  
P. S. Puspita ◽  
A. Syahrom ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Effective Treatment ◽  
Laser Diode ◽  
Ozone Concentration ◽  
Combine Treatment ◽  
Antibacterial Effect ◽  
Irradiation Time ◽  
Photodynamic Inactivation ◽  
Treatment Groups ◽  
Time Lead

Photodynamic inactivation is an effective treatment that uses light irradiation, photosensitizer and oxygen. The aim of this study was to determine photodynamic effectiveness of laser diode combined with ozone to reduce Staphylococcus aureus biofilm using exogenous chlorophyll (Chlo). The chlorophyll was extracted from leave of Dracaena angustifolia. To determine the antibacterial effect of S. aureus biofilm treatments, samples were separated into Chlo, Laser, Chlo+Laser, Ozone, Ozone+Laser, Chlo+Ozone+Laser categories. The data were analyzed using ANOVA test. The result of this study showed that Chlo+Ozone+Laser combine treatment at 20 s exposure of ozone with 4 min of irradiation time lead to 80.26 % reduction of biofilm activity, which was the highest efficacy of all the treatment groups. The combination of laser, chlorophyll and lower ozone concentration increases the effectiveness of photodynamic inactivation.

scholarly journals The efficacy of photodynamic inactivation with laser diode on Staphylococcus aureus biofilm with various ages of biofilm

2020 ◽  
Author(s):  
Suryani Dyah Astuti ◽  
Hafidiana ◽  
Riries Rulaningtyas ◽  
Abdurachman ◽  
Alfian P Putra ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Energy Density ◽  
Diode Laser ◽  
Laser Diode ◽  
Elisa Test ◽  
Control Group ◽  
Photodynamic Inactivation ◽  
Bacterial Cell Death ◽  
Irradiation Energy ◽  
Blue Diode Laser

Biofilms are able to casue microorganisms to be 80% more resistant to antibiotics. The extracelullar polymeric substance (EPS) in biofilm functions to protect bacteria, making it difficult for antibiotics to penetrate the biofilm layer. This study aims to determine the effective- ness of photodynamic inactivation with blue diode laser to reduce Staphylococcus aureus biofilm at various ages of biofilms. The light source is a 403 nm blue diode laser with an energy power of about 27.65±0.01 mW. The study was designed with two groups: Group C was the untreated control group with variations in age of biofilms (0; 6; 11; 17; 24; 32; 40 and 48) hours; Group T was a laser treatment group with variations in age of biofilm and energy density (4.23; 8.46; 12.70; 16.93 and 21.16) J/cm2. Biofilm reduction measurement method using ELISA test was performed to calculate OD595 value. The statistical analysis results of variance showed that there was an influence of biofilm age and irradiation energy density of laser on biofilm reduction. Optical density analysis showed the most optimum biofilm reduction happened when biofilm age is perfectly constructed (about 17 hours) and with 91% reduction. The longer biofilm age lived among those biofilms, the greater the reduction. The results of the Scanning Microscope Electron and fluorescent microscope measurement showed destruction site of the EPS biofilm and bacterial cell death. So, the activated photodynamic with 403 nm laser diode is effective to reduce the Staphylococcus aureus biofilm in the maturation phase.

scholarly journals The photodynamic inactivation of Staphylococcus aureus in water using visible light with a new expanded porphyrin

2012 ◽  
Vol 10 (3) ◽  
pp. 390-399 ◽  
Author(s):  
G. Rossi ◽  
D. Goi ◽  
C. Comuzzi
Keyword(s):  
Staphylococcus Aureus ◽  
Visible Light ◽  
White Light ◽  
Irradiation Time ◽  
Monochromatic Light ◽  
Photodynamic Inactivation ◽  
Light Bulb ◽  
Gram Positive Bacteria ◽  
Expanded Porphyrins ◽  
Expanded Porphyrin

In this work, the results of the application of organic expanded porphyrins in the disinfection of water by the photodynamic inactivation (PDI) technique are presented. The photoinactivation properties of a novel, expanded porphyrin, namely 20-(4-carboxyphenyl)-2,13-dimethyl-3,12-diethyl-(22π) pentaphyrin (PCCox), were tested in the disinfection of water using Staphylococcus aureus as a Gram-positive bacteria model. The data showed that PCCox was effective against S. aureus bacteria at nanomolar concentrations. The variation with irradiation time and concentration was studied using both a multi-LED monochromatic light (λ = 470 nm) and an incandescent light bulb with a wide emission spectrum. A PCCox dosage of 5 μM was sufficient to achieve a 99.997% abatement of S. aureus within 1 h of 40 W/m2 irradiation with monochromatic light (λ = 470 nm), whereas under the same conditions using irradiation with white light, the abatement was 99.9997%.

scholarly journals Cationic, anionic and neutral dyes: effects of photosensitizing properties and experimental conditions on the photodynamic inactivation of pathogenic bacteria

2013 ◽  
Vol 11 (4) ◽  
pp. 590-599 ◽  
Author(s):  
Sonia Sabbahi ◽  
Layla Ben Ayed ◽  
Abdellatif Boudabbous
Keyword(s):  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
Irradiation Time ◽  
Chemical Properties ◽  
Neutral Red ◽  
Viable Count ◽  
Photodynamic Inactivation ◽  
Experimental Conditions ◽  
Significant Difference

The aim of this study was to evaluate the photobactericidal effect of four photosensitizers (PSs) with different structural and physico-photochemical properties, namely mesotetracationic porphyrin (T4MPyP), dianionic rose Bengal (RB), monocationic methylene blue (MB) and neutral red (NR). Their photokilling activity was tested in vitro on pathogenic bacteria such as Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) suspended in nutrient broth (NB) and in phosphate buffered saline (PBS) through following their influence on the PSs antimicrobial efficacy. Photodynamic inactivation (PDI) experiments were performed using visible light (L) and different PSs concentrations (20–70 μM). The ability of these PSs to mediate bacterial photodynamic inactivation was investigated as a function of type of PS and its concentrations, spectral and physico-chemical properties, bacterial strain, irradiation time and suspending medium. Indeed, they showed antibacterial effects against S. aureus and P. aeruginosa with significant difference in potency. Staphylococcus aureus suspended in NB showed 0.92 log units reduction in viable count in the presence of T4MPyP at 20 μM. Changing the suspending medium from NB to PBS, S. aureus was successfully photoinactivated by T4MPyP (20 μM) when suspended in PBS at least time exposure (10 and 30 min), followed by MB and RB.

scholarly journals Synergistic antibacterial combination of Lavandula latifolia Medik. essential oil with camphor

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Nursenem Karaca ◽  
Görkem Şener ◽  
Betül Demirci ◽  
Fatih Demirci
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Antibacterial Effect ◽  
Synergistic Effects ◽  
Pharmaceutical Formulations ◽  
Human Pathogens ◽  
Broth Microdilution ◽  
Antibacterial Effects ◽  
Lavandula Latifolia

AbstractCombination of various compounds and essential oils for pharmaceutical formulations withdraw attention. In this present study, it was aimed to evaluate the in vitro potential synergistic antibacterial effect of Lavandula latifolia (spike lavender) essential oil with camphor by using the checkerboard method against the human pathogens; Staphylococcus aureus and Listeria monocytogenes. Pharmacopoeia quality L. latifolia essential oil and racemic camphor were analyzed and verified by GC-FID and GC/MS, simultaneously. In vitro antibacterial activity of essential oil and camphor (MIC range: 0.16–20 mg/mL) and standard antimicrobial clarithromycin (MIC range: 0.125–16 μg/mL) were carried out by broth microdilution against S. aureus and L. monocytogenes standard strains, respectively. Resulting antibacterial effects were evaluated for their fractional inhibitory concentrations (FICs) as antagonistic, additive and synergistic effects. The analytical results showed that the major component of essential oil was linalool (45.2%) and 1,8-cineole (25.6%). Antibacterial effects of essential oil were determined as MIC 1.25–5 mg/mL. As a result of the experiments, L. latifolia essential oil–camphor combinations were identified as “synergistic (FIC ≤ 0.5), and additive (0.5 < FIC ≤ 1)” in the respective combinations, suggesting further evaluation for formulations for potential antimicrobial applications in food and pharmaceuticals.

scholarly journals Real-Time In Vivo Bioluminescent Imaging for Evaluating the Efficacy of Antibiotics in a Rat Staphylococcus aureus Endocarditis Model

2005 ◽  
Vol 49 (1) ◽  
pp. 380-387 ◽  
Author(s):  
Yan Q. Xiong ◽  
Julie Willard ◽  
Jagath L. Kadurugamuwa ◽  
Jun Yu ◽  
Kevin P. Francis ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Real Time ◽  
Imaging System ◽  
Bioluminescent Imaging ◽  
Vancomycin Therapy ◽  
Treatment Groups ◽  
Highly Sensitive ◽  
Relevant Animal Model ◽  
And Control

ABSTRACT Therapeutic options for invasive Staphylococcus aureus infections have become limited due to rising antimicrobial resistance, making relevant animal model testing of new candidate agents more crucial than ever. In the present studies, a rat model of aortic infective endocarditis (IE) caused by a bioluminescently engineered, biofilm-positive S. aureus strain was used to evaluate real-time antibiotic efficacy directly. This strain was vancomycin and cefazolin susceptible but gentamicin resistant. Bioluminescence was detected and quantified daily in antibiotic-treated and control animals with IE, using a highly sensitive in vivo imaging system (IVIS). Persistent and increasing cardiac bioluminescent signals (BLS) were observed in untreated animals. Three days of vancomycin therapy caused significant reductions in both cardiac BLS (>10-fold versus control) and S. aureus densities in cardiac vegetations (P < 0.005 versus control). However, 3 days after discontinuation of vancomycin therapy, a greater than threefold increase in cardiac BLS was observed, indicating relapsing IE (which was confirmed by quantitative culture). Cefazolin resulted in modest decreases in cardiac BLS and bacterial densities. These microbiologic and cardiac BLS differences during therapy correlated with a longer time-above-MIC for vancomycin (>12 h) than for cefazolin (∼4 h). Gentamicin caused neither a reduction in cardiac S. aureus densities nor a reduction in BLS. There were significant correlations between cardiac BLS and S. aureus densities in vegetations in all treatment groups. These data suggest that bioluminescent imaging provides a substantial advance in the real-time monitoring of the efficacy of therapy of invasive S. aureus infections in live animals.

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