scholarly journals Study the influence of aqueous extract of Lemna minor frond on elemental status in rats with experimental hypothyroidism

2017 ◽  
Vol 0 (2(49)) ◽  
pp. 18-22
Author(s):  
A. Kononenko ◽  
V. Kravchenko
Keyword(s):  
Aqueous Extract ◽  
Lemna Minor ◽  
Elemental Status

scholarly journals HISTOLOGICAL STUDY OF THYROID STATE IN ADMINISTRATION OF AQUEOUS EXTRACT FROM LEMNA MINOR FROND IN EXPERIMENTAL HYPOTHYROIDISM

2016 ◽  
Author(s):  
A. H. H. Kononenko ◽  
V. M. Kravchenko
Keyword(s):  
Thyroid Gland ◽  
Aqueous Extract ◽  
Functional Activity ◽  
Lemna Minor ◽  
Histological Study ◽  
Morphological Structure ◽  
Practical Significance ◽  
Outer Diameter ◽  
Experimental Conditions ◽  
Thyroid State

The influence of aqueous extract from Lemna minor frond on morphological status of the thyroid gland in the experimental conditions of hypothyroidism in rats induced by administration of merkazolilum solution instead of drinking water for 30 days was studied. Thyreostatic merkazolilum resulted in changes of morphological structure of the thyroid gland, accompanied by manifestations of hyperplastic tissue proliferation and corresponds with hypofunctional state.The introduction of aqueous extract from Lemna minor frond resulted in positive influence on the restructuring of the thyroid gland in rats with hypothyroidism, increasing its functional activity. There was a decrease of proliferative processes of the epithelium and extrafollicular thyrocytes in follicles. The outer diameter of the follicles are not different from intact control, height follicular thyrocytes decreased compared with pathology by 41.08%. Iodomarin and L-thyroxine increased functional activity of the thyroid gland, but to a lesser degree than the aqueous extract from Lemna minor frond. L-thyroxine is not increased pharmacological effect of the studied extract. The results have practical significance for drugs development with thyroid-stimulating action.

scholarly journals Biostimulant Effects of an Aqueous Extract of Duckweed (Lemna minor L.) on Physiological and Biochemical Traits in the Olive Tree

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1299
Author(s):  
Luca Regni ◽  
Daniele Del Buono ◽  
Begoña Miras-Moreno ◽  
Biancamaria Senizza ◽  
Luigi Lucini ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Aqueous Extract ◽  
Plant Biomass ◽  
Growth Parameters ◽  
Lemna Minor ◽  
Net Photosynthesis ◽  
Dry Weight ◽  
Co2 Concentration ◽  
Olive Tree ◽  
Water Volume

Biostimulants are becoming increasingly popular in agriculture for their ability to induce beneficial effects in crops, paving the way towards the identification of new materials with biostimulant potential. This study evaluated the potential of different concentrations of an aqueous extract (0.25%, 0.50%, and 1.00%, dry weight/water volume, respectively) obtained from duckweed (Lemna minor L.) to stimulate olive plants. Leaf net photosynthesis (Pn), leaf transpiration rate (E), stomatal conductance (gs), sub-stomatal CO2 concentration (Ci), chlorophyll content and other plant growth parameters were investigated. As a result, the extract improved Pn, gs, Ci, chlorophyll content and plant biomass production (leaf fresh and dry weight). Furthermore, the duckweed extract generally increased the uptake of nitrogen (N), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe) and zinc (Zn), while it did not influence the content of sodium (Na), manganese (Mn) and copper (Cu). The untargeted metabolomic profiling of the extract revealed the presence of signalling compounds (including phytohormones), phenolics and glutathione. Such broad diversity of bioactives may support the stimulatory potential observed in olive. In summary, this study revealed for the first time that duckweed could be seen as a promising species to obtain extracts with biostimulant properties in olive trees.

scholarly journals Physiological and Biochemical Effects of an Aqueous Extract of Lemna minor L. as a Potential Biostimulant for Maize

2021 ◽  
Author(s):  
Daniele Del Buono ◽  
Maria Luce Bartucca ◽  
Eleonora Ballerini ◽  
Biancamaria Senizza ◽  
Luigi Lucini ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Growth Traits ◽  
Lemna Minor ◽  
Dry Weight ◽  
Water Volume ◽  
Freshwater Species ◽  
Beneficial Effects ◽  
Maize Seeds ◽  
Controlled Conditions ◽  
Physiological And Biochemical

AbstractBiostimulants are receiving increasing attention for their beneficial effects on crops, driving interest in identifying new plant extracts that could exert such stimulatory effects. This work aimed to evaluate the potential of an aqueous extract obtained from duckweed (Lemna minor L.), a freshwater species, to act as a biostimulant in maize. For this purpose, duckweed plants were collected from a natural basin and then transferred, stabilized, and grown under controlled conditions. The duckweed extract was first characterized through untargeted profiling, which revealed an abundance of bioactive phytochemicals. A relatively high amount of low-molecular-weight secondary metabolites such as phenolics (6714.99 mg kg−1) and glucosinolates (4563.74 mg kg−1) were present in the plant extract. Maize seeds were primed with different concentrations of this extract (0.01%, 0.05%, 0.50%, and 1.00%, dry weight/water volume), and some physiological and biochemical traits of the crop were recorded. The duckweed extract improved maize germination, biomass, leaf area, pigment content, and vigor index. The most effective treatment was the 0.50% concentration, which improved the majority of the measured growth traits. The extract at concentrations of 0.05%, 0.50%, and 1.00% stimulated the assimilation of nitrogen (N), phosphorous (P), potassium (K), calcium (Ca), magnesium (Mg), sodium (Na), iron (Fe), and copper (Cu). In summary, this study revealed that duckweed is a promising species that can be cultured and grown under controlled conditions for obtaining extracts with biostimulant properties.

scholarly journals CORRECTING EFFECT OF AQUEOUS EXTRACT FROM DUCKWEED LEMNA MINORFROND ON MORPHOLOGICAL STATE OF THYROID GLAND IN RATS WITH HYPOTHYROIDISM

2016 ◽  
Vol 2 ◽  
pp. 3-9
Author(s):  
Alevtyna Kononenko ◽  
Vera Kravchenko
Keyword(s):  
Thyroid Gland ◽  
Aqueous Extract ◽  
Lemna Minor ◽  
Sodium Perchlorate ◽  
Practical Significance ◽  
Albino Rats ◽  
Outer Diameter ◽  
Reference Drug ◽  
Functional Changes ◽  
Morphological State

The present work studied the effect of aqueous extract from duckweed Lemna minor frond on morphological state of the thyroid gland in the experimental hypothyroidism. Healthy albino rats weighing between 120 g and 150 g were used. The animalswere randomly allotted into four groups, each containing ten rats respectively. Three of the groups (II, III and IV) induced with hypothyroidism by 1 % solution of sodium perchlorate with drinking water for 20 days. Control (vehicle) rats were given normal saline. After 20 days hypothyroid groups (III and IV) of rats treated with aqueous extract from duckweed Lemna minor frond at a dose 0.5 ml/100 g of body weight and with Iodomarin 200 (reference drug) at a dose 12 µg/kg daily orally for 21 days. Results obtained from the study showed that the introduction of sodium perchlorate leads to morphological and functional changes in the structure of the thyroid gland in rats, accompanied by sings of hyperplastic tissue proliferation and corresponds with hypofunctional state. It was established that introduction of aqueous extract from duckweed Lemna minor frond has a positive effect on the restructuring of the thyroid gland in rats with hypothyroidism, increasing its functional activity. There is a decrease of proliferative processes by extrafolliсular epithelium and thyrocytes in the follicles. The outer diameter of follicles decreased by 7.65 % (P>0.05), the height and size of thyrocytes in the wall of the follicle were significantly decreased by 58.16 % and 18 41 %, respectively, compared to untreated hypothyroid group. The obtained results have practical significance for the development of drugs with thyroid-stimulating action for preventing or delaying the development of hypothyroidism and its complications.

Low temperature x-ray microanalysis of frozen-hydrated tobacco leaves

1984 ◽  
Vol 42 ◽  
pp. 284-285
Author(s):  
S. Edith Taylor ◽  
Patrick Echlin ◽  
May McKoon ◽  
Thomas L. Hayes
Keyword(s):  
Low Temperature ◽  
Lemna Minor ◽  
Root Tips ◽  
Tobacco Leaves ◽  
X Ray ◽  
Whole Cells ◽  
Carbon Coated ◽  
The Right ◽  
Beam Currents ◽  
The University

Low temperature x-ray microanalysis (LTXM) of solid biological materials has been documented for Lemna minor L. root tips. This discussion will be limited to a demonstration of LTXM for measuring relative elemental distributions of P,S,Cl and K species within whole cells of tobacco leaves.Mature Wisconsin-38 tobacco was grown in the greenhouse at the University of California, Berkeley and picked daily from the mid-stalk position (leaf #9). The tissue was excised from the right of the mid rib and rapidly frozen in liquid nitrogen slush. It was then placed into an Amray biochamber and maintained at 103K. Fracture faces of the tissue were prepared and carbon-coated in the biochamber. The prepared sample was transferred from the biochamber to the Amray 1000A SEM equipped with a cold stage to maintain low temperatures at 103K. Analyses were performed using a tungsten source with accelerating voltages of 17.5 to 20 KV and beam currents from 1-2nA.

Elemental Analysis of Phloem Tissue in Lemna Minor Root Tips

1980 ◽  
Vol 38 ◽  
pp. 798-799
Author(s):  
Patrick Echlin ◽  
Thomas Hayes ◽  
Clifford Lai ◽  
Greg Hook
Keyword(s):  
Vascular Tissue ◽  
Lemna Minor ◽  
Atomic Weight ◽  
Companion Cell ◽  
Root Tips ◽  
Phloem Tissue ◽  
Cell Stage ◽  
Phloem Parenchyma ◽  
Parenchyma Cells ◽  
Xylem Element

Studies (1—4) have shown that it is possible to distinguish different stages of phloem tissue differentiation in the developing roots of Lemna minor by examination in the transmission, scanning, and optical microscopes. A disorganized meristem, immediately behind the root-cap, gives rise to the vascular tissue, which consists of single central xylem element surrounded by a ring of phloem parenchyma cells. This ring of cells is first seen at the 4-5 cell stage, but increases to as many as 11 cells by repeated radial anticlinal divisions. At some point, usually at or shortly after the 8 cell stage, two phloem parenchyma cells located opposite each other on the ring of cells, undergo an unsynchronized, periclinal division to give rise to the sieve element and companion cell. Because of the limited number of cells involved, this developmental sequence offers a relatively simple system in which some of the factors underlying cell division and differentiation may be investigated, including the distribution of diffusible low atomic weight elements within individual cells of the phloem tissue.

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