scholarly journals Local Expression of Interleukin-17a is Correlated with Nasal Eosinophilia and Clinical Severity in Allergic Rhinitis

2014 ◽  
Vol 5 (1) ◽  
pp. ar.2014.5.0078 ◽  
Author(s):  
Seiichiro Makihara ◽  
Mitsuhiro Okano ◽  
Tazuko Fujiwara ◽  
Yohei Noda ◽  
Takaya Higaki ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Mucosa ◽  
Immunoglobulin E ◽  
Inferior Turbinate ◽  
Forced Expiratory Volume ◽  
Clinical Severity ◽  
Eosinophil Infiltration ◽  
Total Serum ◽  
Blood Eosinophil ◽  
Local Expression

Interleukin (IL)-17A is a major cytokine produced by Th17 cells, which are associated with chronic inflammations. The local expression of IL-17A in allergic rhinitis (AR) remains to be characterized. We sought to determine the role of IL-17A expression in human inferior turbinate mucosa in the pathophysiology of AR. Inferior turbinate mucosa was sampled from medical treatment-resistant, surgery-required patients with perennial AR (PAR, n = 21), nonallergic rhinitis with eosinophilia syndrome (NARES, n = 7), and nonallergic hypertrophic rhinitis (HR, n = 13). IL-17A expression was determined with immunohistochemical staining. The mean number of IL-17A+ cells and eosinophils per field were counted. Total serum immunoglobulin E (IgE) levels, blood eosinophil count, and forced expiratory volume in 1 second (FEV1)/forced vital capacity (FVC) ratio were also examined in each patient. IL-17A was primarily expressed in infiltrating inflammatory cells. The number of IL-17A+ cells in nasal mucosa was significantly higher in the PAR group compared with HR (p = 0.002) and NARES (p = 0.021) groups. There was a significant and positive correlation between the number of IL-17A+ cells and total nasal symptom score (rho = 0.403; p = 0.011), especially sneezing score (rho = 0.471; p = 0.003). The number of IL-17A+ cells was significantly and positively correlated with the degree of eosinophil infiltration (rho = 0.623; p < 0.001), but not with total serum IgE levels (rho = 0.284; p = 0.098), blood eosinophil counts (rho = 0.302; p = 0.056), or FEV1/FVC ratio (rho = 0.092; p = 0.569). The present study provides evidence that IL-17A expression in the nasal mucosa is associated with the pathophysiology of AR, including disease severity and nasal eosinophilia.

scholarly journals The Study of a Possible Correlation between Serum Levels of Interleukin 17 and Clinical Severity in Patients with Allergic Rhinitis

2017 ◽  
Vol 8 (3) ◽  
pp. ar.2017.8.0207
Author(s):  
Mai Aly Gharib Aly ◽  
Mohamed Tawfik El Tabbakh ◽  
Waheed Fawzy Heissam ◽  
Said Hamed Abbadi
Keyword(s):  
Allergic Rhinitis ◽  
Immunoglobulin E ◽  
Skin Testing ◽  
Allergic Diseases ◽  
Serum Levels ◽  
Clinical Severity ◽  
Total Serum ◽  
Interleukin 17 ◽  
Serum Ige ◽  
Cluster Immunotherapy

Introduction Allergic rhinitis (AR) is one of the most common allergic diseases, which affects ~20% of the world's population. T-helper (Th) type 2 cells produce interleukin (IL) 4 and IL-13, and mediate allergic responses, and these cytokines have been extensively studied as key players in the atopic airway diseases. However, the involvement of Th17 cells and IL-17 in AR has not been clearly examined. Aim To reevaluate AR clinical severity with serum IL-17, whether IL-17 affects the disease alone or in contribution with the atopic predisposition. Patients and Methods During an 18-month period, 39 individuals were divided into three groups: A, (13 control), B (13 with mild-to-moderate AR), and C (13 with severe AR). Both group B and group C patients (26) were subjected to clinical examination and allergy skin testing, and to measurement of both total serum immunoglobulin E (IgE) and IL-17 levels. Eleven patients with AR then were exposed to 6 months of cluster immunotherapy, whereas the rest of the patients were not exposed. Results Revealed a significant elevation of serum IL-17 levels with an associated increase in serum IgE in the patients with AR compared with controls and revealed that the serum levels of both total serum IgE and IL-17 decreased significantly after cluster immunotherapy. Conclusion These preliminary results added new data about the use of injective immunotherapy as well as reported on the use of sublingual immunotherapy.

scholarly journals Traditional Chinese MedicineZHENGIdentification Provides a Novel Stratification Approach in Patients with Allergic Rhinitis

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Kai-Li Liang ◽  
Rong-San Jiang ◽  
Chia-Lin Lee ◽  
Pei-Jung Chiang ◽  
Jui-Shan Lin ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Immunoglobulin E ◽  
Linear Regression Analysis ◽  
Eosinophil Cationic Protein ◽  
Multiple Linear Regression Analysis ◽  
Total Serum ◽  
Blood Eosinophil ◽  
Cationic Protein ◽  
Explanatory Factors ◽  
Severity Scores

Background. We aimed to apply theZHENGidentification to provide an easy and useful tool to stratify the patients with allergic rhinitis (AR) through exploring the correlation between the quantified scores of AR symptoms and the TCMZHENGs.Methods. A total of 114 AR patients were enrolled in this observational study. All participants received the examinations of anterior rhinoscopy and acoustic rhinometry. Their blood samples were collected for measurement of total serum immunoglobulin E (IgE), blood eosinophil count (Eos), and serum eosinophil cationic protein (ECP). They also received two questionnaire to assess the severity scores of AR symptoms and quantified TCMZHENGscores. Multiple linear regression analysis was used to determine explanatory factors for the score of AR manifestations.Results. IgE and ECP level, duration of AR, the 2 derived TCMZHENGscores of “Yin-Xu − Yang-Xu”, and “Qi-Xu+Blood-Xu” were 5 explanatory variables to predict the severity scores of AR symptoms. The patients who had higher scores of “Yin-Xu − Yang-Xu” or “Qi-Xu+Blood-Xu” tended to manifest as “sneezer and runner” or “blockers,” respectively.Conclusions.The TCMZHENGscores correlated with the severity scores of AR symptoms and provided an easy and useful tool to stratify the AR patients.

scholarly journals Measuring local immunoglobulin E in the inferior turbinate nasal mucosa in patients with allergic rhinitis

2016 ◽  
Vol 65 (4) ◽  
pp. 396-399 ◽  
Author(s):  
Yasushi Ota ◽  
Yoshihiro Ikemiyagi ◽  
Toshiya Sato ◽  
Tatsuo Funakoshi ◽  
Nobuyuki Hiruta ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Mucosa ◽  
Immunoglobulin E ◽  
Inferior Turbinate

Nasal Cytology in Radiofrequency Turbinate Volume Reduction

ORL ◽  
2021 ◽  
pp. 1-6
Author(s):  
Giancarlo Pecorari ◽  
Giuseppe Riva ◽  
Claudia Bartoli ◽  
Mattia Ravera ◽  
Valeria Dell’Era ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Effective Treatment ◽  
Nasal Obstruction ◽  
Nasal Mucosa ◽  
Inferior Turbinate ◽  
Volume Reduction ◽  
Nonallergic Rhinitis ◽  
Turbinate Hypertrophy ◽  
Nasal Cytology ◽  
Rhinitis Medicamentosa

Introduction: Radiofrequency turbinate volume reduction (RFTVR) is an effective treatment of inferior turbinate hypertrophy. RFTVR can reduce epithelial cell alterations in nasal mucosa. The aim of this observational study was to evaluate the effects of RFTVR on nasal obstruction and cytology, stratifying for different types of rhinitis. Methods: Nasal cytology and subjective nasal obstruction were evaluated on 113 patients before RFTVR (T0) and after 3 months (T1). The patients were divided into groups on the basis of the underlying disease: allergic rhinitis, nonallergic rhinitis, rhinitis medicamentosa, and other diseases (e.g., hormonal-based turbinate hypertrophy). Results: Nasal cytology at T0 identified 42 patients with allergic rhinitis, 40 with nonallergic rhinitis, 19 with rhinitis medicamentosa, and 12 with other diseases. An improvement of nasal cytology at T1 was observed in 29.2% of cases. They mainly consisted of patients with nonallergic rhinitis with neutrophils, whose neutrophil infiltrate decreased. Only 2 cases (1.7%) showed a worsening of nasal cytology at T1. A statistically significant decrease in subjective nasal obstruction was observed for every group (p < 0.05). Higher differences of nasal obstruction between T0 and T1 were found in patients with rhinitis medicamentosa or other diseases. Conclusion: RFTVR represents a safe and effective treatment for turbinate hypertrophy of various etiology. It is not responsible for a worsening of inflammatory infiltrate of the nasal mucosa.

scholarly journals Local allergic rhinitis

2014 ◽  
Vol 67 (suppl. 1) ◽  
pp. 49-56
Author(s):  
Maja Buljcik-Cupic ◽  
Dragana Lemez ◽  
Slobodan Savovic ◽  
Ljiljana Jovancevic ◽  
Danijela Dragicevic
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Mucosa ◽  
Immunoglobulin E ◽  
Provocation Test ◽  
Allergic Response ◽  
Prick Test ◽  
Local Allergic Rhinitis ◽  
Provocation Tests ◽  
Number Of Patients ◽  
The Impact

Local allergic rhinitis is a localized allergic response of the nasal mucosa to aeroallergens in the absence of atopy with characteristic production of specific local immunoglobulin E antibodies in the nasal mucosa, T helper type 2 cellular infiltration response during the exposure to aeroallergens and positive results via the nasal allergic provocation test with the release of inflammatory mediators (triptase and eosinophil cationic proteins). Even though the prevalence of local allergic rhinitis has been and is still being investigated, a large number of patients with diagnosed non-allergic rhinitis or idiopathic rhinitis are currently classified as having local allergic rhinitis. The causes of local allergic rhinitis are most commonly house dust, dust mites, pollens and many others. Diagnosis of local allergic rhinitis is made using nasal allergen provocation tests when the prick test for standard inhalation allergens and the serum specific immunoglobulin analysis for aeroallergens are negative. The increasing amount of data on localized allergic response in non-atopic patients asks for many answers regarding local allergic rhinitis. These answers can be obtained by a study on the prevalence and incidence in children and adults, the impact of positive family atopy in the development of disease, the impact of associated diseases of the lower respiratory tract and conjunctiva, the effectiveness of drug treatment and the issue of administration of specific immunotherapy.

scholarly journals Multifaceted Analyses of Epidermal Serine Protease Activity in Patients with Atopic Dermatitis

2020 ◽  
Vol 21 (3) ◽  
pp. 913 ◽  
Author(s):  
Hayato Nomura ◽  
Mutsumi Suganuma ◽  
Takuya Takeichi ◽  
Michihiro Kono ◽  
Yuki Isokane ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Serine Protease ◽  
Protease Activity ◽  
Serine Proteases ◽  
Immunoglobulin E ◽  
Total Serum ◽  
Tape Stripping ◽  
Blood Eosinophil ◽  
Peripheral Blood Eosinophil ◽  
Serine Protease Activity

The serine proteases kallikrein-related peptidase (KLK) 5 and KLK7 cleave cell adhesion molecules in the epidermis. Aberrant epidermal serine protease activity is thought to play an important role in the pathogenesis of atopic dermatitis (AD). We collected the stratum corneum (SC) from healthy individuals (n = 46) and AD patients (n = 63) by tape stripping and then measuring the trypsin- and chymotrypsin-like serine protease activity. We also analyzed the p.D386N and p.E420K of SPINK5 variants and loss-of-function mutations of FLG in the AD patients. The serine protease activity in the SC was increased not only in AD lesions but also in non-lesions of AD patients. We found, generally, that there was a positive correlation between the serine protease activity in the SC and the total serum immunoglobulin E (IgE) levels, serum thymus and activation-regulated chemokine (TARC) levels, and peripheral blood eosinophil counts. Moreover, the p.D386N or p.E420K in SPINK5 and FLG mutations were not significantly associated with the SC’s serine protease activity. Epidermal serine protease activity was increased even in non-lesions of AD patients. Such activity was found to correlate with a number of biomarkers of AD. Further investigations of serine proteases might provide new treatments and prophylaxis for AD.

scholarly journals Frequency of Raised Total Serum Ig E, Sputum Eosinophilia and Blood Eosinophil Count in Patients with Severe Persistent Asthma

2021 ◽  
Vol 35 (1) ◽  
Author(s):  
Shanawer Qaiser
Keyword(s):  
Eosinophil Count ◽  
Inflammatory Markers ◽  
Immunoglobulin E ◽  
Persistent Asthma ◽  
Serum Immunoglobulin ◽  
Total Serum ◽  
Blood Eosinophil ◽  
Blood Eosinophil Count ◽  
Sputum Eosinophilia ◽  
Severe Persistent Asthma

Introduction: Asthma is a chronic inflammatory airway disorder that has several inflammatory phenotypes. Serum immunoglobulin E (IgE) and eosinophils are airway inflammation markers in asthmatic patients. In this research, we assessed the asthma severity with various inflammatory markers. Aims & Objectives: To find the frequency of raised total serum immunoglobulin E, sputum eosinophilia, and absolute blood eosinophil count in patients with severe persistent asthma. Place and duration of study: This study was conducted in Department of Pulmonology, Shaikh Zayed FPGMI, Lahore from 1st October 2018 to 1st April 2019. Material & Methods: A Cross-sectional study in which total of 125 patients were enrolled after fulfilling the inclusion criteria. Blood samples were taken with aseptic measures for total serum immunoglobulin E and absolute blood eosinophils count. Sputum was collected in the sterile jar and dispensed properly to the histopathology lab. Results: Of 125 patients, 44.8% were males, and 55.2% were females. The mean patient's age was 49.37 years, and the mean duration of symptoms was 25.49 years. Raised serum immunoglobulin E levels were seen in 74.4% patients, sputum eosinophilia was seen in 25.6% patients, and increased peripheral eosinophil count was seen in 55.2%. Age was significantly associated with sputum eosinophilia; however, no such association was seen between the other effect modifiers and inflammatory markers. Conclusion: Inflammatory markers immunoglobulin E, sputum and blood eosinophil levels were significantly raised in asthma patients. These can be used in the detection of asthma as their detection is easy, simple, and non-invasive, and they are directly linked to the inflammation.

LncRNA MIAT Promotes Allergic Inflammation and Symptoms by Targeting MiR-10b-5p in Allergic Rhinitis Mice

2021 ◽  
Vol 35 (6) ◽  
pp. 781-789
Author(s):  
Zhiqi Ma ◽  
Haijuan Lian ◽  
Xiaoyan Lin ◽  
Yong Li
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Mucosa ◽  
Th17 Cells ◽  
Immunoglobulin E ◽  
Allergic Inflammation ◽  
Specific Ige ◽  
Luciferase Reporter ◽  
Mice Model ◽  
Ige Response ◽  
The Relationship

Background Allergic rhinitis (AR) is one of the most common noninfectious respiratory diseases caused by immunoglobulin E (IgE) response. Objective The study sought to explore the relationship between lncRNA MIAT and miR-10b-5p and their interaction in the regulation of allergic phenotypes in allergic rhinitis (AR) mice. Methods A mice model of AR was constructed using ovalbumin (OVA) sensitization. AR mice were treated with miR-10b-5p agomiR and LNA mediated lncRNA MIAT. The targeting relationship between MIAT and miR-10b-5p was analyzed by the ENCORI website and dual-luciferase reporter assay. The numbers of rubbing and sneezing of mice were counted. Hematoxylin-eosin (HE) staining visualized the eosinophils infiltration in nasal mucosa tissues of mice. The percentage of Th17 cells was quantitated by flow cytometry analysis. ELISA was used to detect the levels of serum OVA-specific IgE, the Th12 cytokine IL-4, and inflammatory cytokines (IL-6, IL-17). Results MIAT was up-regulated in the nasal mucosa of AR mice, while miR-10b-5p was down-regulated. MIAT directly suppressed miR-10b-5p expression in AR mice. The numbers of rubbing and sneezing, the percentage of Th17 cells, and the levels of OVA-specific IgE, IL-4, IL-6, and IL-17 in AR mice were decreased by miR-10b-5p overexpression, which was reversed by MIAT overexpression. The eosinophils infiltration in AR mice was inhibited by miR-10b-5p overexpression, which was also reversed by MIAT overexpression. Conclusion The present study demonstrates that MIAT overexpression Promotes allergic inflammation and symptoms by activating Th17 immune response via miR-10b-5p inhibition.

scholarly journals TLR4 and TLR9 Expression in Different Phenotypes of Rhinitis

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Maria Lauriello ◽  
Alessandra Micera ◽  
Paola Muzi ◽  
Lino Di Rienzo Businco ◽  
Sergio Bonini
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Mucosa ◽  
Inferior Turbinate ◽  
Vasomotor Rhinitis ◽  
Tlr9 Expression ◽  
Persistent Allergic Rhinitis ◽  
Evolutionarily Conserved ◽  
Chronic Rhino Sinusitis ◽  
Nasal Inflammation ◽  
Severity Of The Disease

Background. Toll-like receptors (TLRs) represent a family of evolutionarily conserved proteins, that represent a fundamental link between innate and adaptive immune responses.Aim. The purpose of this study was to investigate the expression of TLR4 and TLR9 in the normal nasal mucosa and in the mucosa of subjects with different phenotypes of rhinitis.Methodology. A confocal analysis of TLR4 and TLR9 (co)expression was carried out on biopsies from the inferior turbinate obtained from 4 patients affected by persistent allergic rhinitis, 8 patients with chronic rhino-sinusitis, and 6 patients with vasomotor rhinitis The results were compared with those of specimens obtained from 4 subjects undergoing nasal surgery, but with signs of nasal inflammation.Results. TLR4 and TLR9 were expressed in the healthy nasal mucosa; TLR4 and TLR9 expression was significantly decreased in allergic rhinitis. TLR4 was over expressed in the epithelium of chronic rhino-sinusitis. Both TLRs were co-expressed in the sub-epithelial infiltrate of chronic and vasomotor rhinitis, even though this expression was higher in the former compared with the latter.Conclusions. This study indicates that TLR4 and TLR9 show a different pattern of expression in different phenotypes of rhinitis, possibly related to the type and severity of the disease.

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