The Study of a Possible Correlation between Serum Levels of Interleukin 17 and Clinical Severity in Patients with Allergic Rhinitis

Introduction Allergic rhinitis (AR) is one of the most common allergic diseases, which affects ~20% of the world's population. T-helper (Th) type 2 cells produce interleukin (IL) 4 and IL-13, and mediate allergic responses, and these cytokines have been extensively studied as key players in the atopic airway diseases. However, the involvement of Th17 cells and IL-17 in AR has not been clearly examined. Aim To reevaluate AR clinical severity with serum IL-17, whether IL-17 affects the disease alone or in contribution with the atopic predisposition. Patients and Methods During an 18-month period, 39 individuals were divided into three groups: A, (13 control), B (13 with mild-to-moderate AR), and C (13 with severe AR). Both group B and group C patients (26) were subjected to clinical examination and allergy skin testing, and to measurement of both total serum immunoglobulin E (IgE) and IL-17 levels. Eleven patients with AR then were exposed to 6 months of cluster immunotherapy, whereas the rest of the patients were not exposed. Results Revealed a significant elevation of serum IL-17 levels with an associated increase in serum IgE in the patients with AR compared with controls and revealed that the serum levels of both total serum IgE and IL-17 decreased significantly after cluster immunotherapy. Conclusion These preliminary results added new data about the use of injective immunotherapy as well as reported on the use of sublingual immunotherapy.

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Dose-Dependent Effect of Cotinine-Verified Tobacco Smoking on Serum Immunoglobulin E Levels in Korean Adult Males

Nicotine & Tobacco Research ◽

10.1093/ntr/ntx247 ◽

2017 ◽

Vol 21 (6) ◽

pp. 813-817 ◽

Cited By ~ 3

Author(s):

Jae-June Dong ◽

Jay J Shen ◽

Yong-Jae Lee

Keyword(s):

Tobacco Smoking ◽

Immunoglobulin E ◽

Allergic Diseases ◽

Serum Immunoglobulin ◽

Total Serum ◽

Adult Males ◽

Serum Ige ◽

Korean Adult ◽

Dose Dependent ◽

Dose Dependent Effect

Abstract Background Smoking is one of the risk factors to exacerbate allergic diseases, and it may affect serum immunoglobulin E (IgE) levels. However, few studies have relied on an objective biomarker to examine the effect of tobacco smoking on serum IgE levels. Method A nationwide cross-sectional study was conducted to examine the relationship between urinary cotinine (Ucot) concentrations and IgE levels in 973 males using data from the 2010 Korean National Health and Nutrition Examination Survey (KNHANES). Ucot was classified into four groups based on concentration (ng/mL) as follows: nonsmoker group (Ucot <50 ng/mL) and three tertile groups in smokers (T1 [Ucot: 50.00–921.28 ng/mL]; T2 [Ucot: 921.29–1869.36 ng/mL]; and T3 [Ucot ≥1869.37 ng/mL]). The dose-response relationships between Ucot concentrations and total serum IgE level were estimated using analysis of covariance (ANCOVA) and multiple linear regression analysis after adjusting for confounding variables. Results We found a significant and positive dose-related effect of cigarette smoking as measured by Ucot concentrations on the total serum IgE level. The multivariate adjusted means of total serum IgE levels (SE) were 321.0 (36.3), 404.4 (102.7), 499.2 (79.2), and 534.7 (82.7) IU/mL, after adjusting for age, body mass index, alcohol ingestion, physical exercise, job, and household income. The regression coefficient β for total serum IgE was β = 68.6 with increasing level of Ucot group after adjusting for the same covariables (p = .009). Conclusion These findings suggest that the amount of smoking may have a dose-dependent effect on total serum IgE levels. Implication Smoking is one of the risk factors to exacerbate allergic diseases, and it may affect serum immunoglobulin E (IgE) levels, which is closely related to type 1 mediated allergic diseases. However, few studies have relied on an objective biomarker to examine the effect of tobacco smoking on serum IgE levels. We found that tobacco exposure, as measured by Ucot concentrations, increased the serum IgE levels in a dose-response manner in a representative sample of Korean adult males.

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Comparison of 1α-25-dihydroxyvitamin D3 and IgE Levels between Allergic Rhinitis Patients and Healthy People

International Archives of Otorhinolaryngology ◽

10.1055/s-0038-1649492 ◽

2018 ◽

Vol 22 (04) ◽

pp. 428-431

Author(s):

Mehmet Demir

Keyword(s):

Allergic Rhinitis ◽

Immunoglobulin E ◽

Allergic Diseases ◽

Serum Levels ◽

Mean Value ◽

Lifestyle Changes ◽

Healthy Population ◽

Dihydroxyvitamin D3 ◽

Control Subjects ◽

Skin Prick Tests

Introduction Allergic rhinitis is one of the most common allergic diseases that cause a decrease in quality of life. Over the last decades, the increase in the number of cases is supposed to be a result of industrialization and lifestyle changes. Vitamin D, which is a steroid hormone, has a significant immunomodulatory and antioxidant role in the human body. Objective The objective of the present study is to investigate the role of 1α-25-dihydroxyvitamin D3 levels in allergic rhinitis patients and to compare them to the levels found in the healthy population. Methods A total of 256 participants were included in the present study. Allergic rhinitis is diagnosed with the help of the Allergic Rhinitis and Its Impact on Asthma (ARIA) guidelines confirmed through skin prick tests. Control subjects were selected among people without allergy history, which was also confirmed through skin prick tests. The levels of 1α-25-dihydroxyvitamin D3, immunoglobulin E (IgE), and eosinophil counts were measured in blood samples from both groups. Results The mean value of serum levels of 1α-25-hydroxyvitamin D3 was 25.5 ± 3.74 in the allergic rhinitis group, and 31.58 ± 3.85 in the control subjects. This difference reveals statistically-decreased levels in the allergic rhinitis group (p < 0.05). The total IgE levels are increased in the allergic rhinitis group (p < 0.05) and negatively correlated with the serum levels of 1α-25-hydroxyvitamin D3 (r = - 0.259, p < 0.05). Conclusion Lower serum levels of 1α-25-hydroxyvitamin D3 were detected in the allergic rhinitis population. This data is also correlated to the IgE response in the study group. A supplement of 1α-25-hydroxyvitamin D3 in deficient patients might be helpful to relieve symptoms and signs of allergic rhinitis, but further studies are needed.

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Association between Type 2 Diabetes, Obesity and Key Immunological Components of IgE-mediated Inflammation

Folia Medica ◽

10.1515/folmed-2017-0021 ◽

2017 ◽

Vol 59 (2) ◽

pp. 159-164 ◽

Cited By ~ 3

Author(s):

Raditsa N. Sokolova ◽

Rumyana K. Yankova ◽

Tsvetana I. Abadjieva ◽

Tatyana A. Popova ◽

Maria V. Ivanovska ◽

...

Keyword(s):

Type 2 Diabetes ◽

Glycemic Control ◽

Allergic Inflammation ◽

Allergic Diseases ◽

Serum Levels ◽

Total Serum ◽

Control Group ◽

Serum Ige ◽

Ige Mediated

AbstractBackground:Changes in lifestyle and obesity in recent decades have brought about a dramatic increase in type 2 diabetes mellitus (DM2) and allergic diseases. Clinical and epidemiological studies associate obesity with epidemics of allergic diseases. The link between obesity and DM2 with immunological components of IgE-mediated allergic inflammation is not yet conclusively established.Aim:To examine the key immunological components of IgE-mediated allergic inflammation in patients with DM2 and their relationship with glycemic control and anthropometric indicators.Materials and methods:Fifty-five patients with DM2 and 32 healthy controls with normal weight and body mass index (BMI) of 18-24.9 kg/m2were included in the study. Th2-cytokine profile (serum levels of IL-4 and IL-5, pg/ml) and total serum IgE IU/ml were assessed in all participants in the study using ELISA. In patients with DM2, levels of glycated hemoglobin (HbA1c%) in the blood were also measured.Results:Serum levels of IL-4 and IL-5 are significantly higher in patients with DM2 compared to the control group. Serum levels of IL-4 and IL-5 positively correlated with BMI as well as serum levels of IL-4 with waist circumference. Total serum IgE positively correlated with HbA1c.Conclusion:Obesity and poor glycemic control in patients with DM2 affect key immunological components of IgE-mediated allergic inflammation and possibly alter the immune response to allergens and antigens.

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DNA Methylation at Birth is Associated with Childhood Serum Immunoglobulin E Levels

Epigenetics Insights ◽

10.1177/25168657211008108 ◽

2021 ◽

Vol 14 ◽

pp. 251686572110081

Author(s):

Luhang Han ◽

Akhilesh Kaushal ◽

Hongmei Zhang ◽

Latha Kadalayil ◽

Jiasong Duan ◽

...

Keyword(s):

Dna Methylation ◽

Gene Ontology ◽

Cord Blood ◽

Early Life ◽

Immunoglobulin E ◽

Allergic Diseases ◽

Total Serum ◽

Gene Ontology Analysis ◽

Serum Ige ◽

Cpg Sites

Immunoglobulin E (IgE) is known to play an important role in allergic diseases. Epigenetic traits acquired due to modification of deoxyribonucleic acid (DNA) methylation (DNAm) in early life may have phenotypic consequences through their role in transcriptional regulation with relevance to the developmental origins of diseases including allergy. However, epigenome-scale studies on the longitudinal association of cord blood DNAm with IgE over time are lacking. Our study aimed to examine the association of DNAm at birth with childhood serum IgE levels during early life. Genome-scale DNAm and total serum IgE measured at birth, 5, 8, and 11 years of children in the Taiwan Maternal and Infant Cohort Study were included in the study in the discovery stage. Linear mixed models were implemented to assess the association between cord blood DNAm at ~310K 5′-cytosine-phosphate-guanine-3′ (CpG) sites with repeated IgE measurements, adjusting for cord blood IgE. Identified statistically significant CpGs (at a false discovery rate, FDR, of 0.05) were further tested in an independent replication cohort, the Isle of Wight (IoW) birth cohort. We mapped replicated CpGs to genes and conducted gene ontology analysis using ToppFun to identify significantly enriched pathways and biological processes of the genes. Cord blood DNAm of 273 CpG sites were significantly (FDR = 0.05) associated with IgE levels longitudinally. Among the identified CpGs available in both cohorts (184 CpGs), 92 CpGs (50%) were replicated in the IoW in terms of consistency in direction of associations between DNA methylation and IgE levels later in life, and 16 of the 92 CpGs showed statistically significant associations ( P < .05). Gene ontology analysis identified 4 pathways (FDR = 0.05). The identified 16 CpG sites had the potential to serve as epigenetic markers associated with later IgE production, beneficial to allergic disease prevention and intervention.

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Does aeroallergen sensitivity and allergic rhinitis in children cause milder COVID-19 infection?

Allergy and Asthma Proceedings ◽

10.2500/aap.2021.42.210087 ◽

2021 ◽

Vol 42 (6) ◽

pp. 522-529 ◽

Cited By ~ 1

Author(s):

Emine Vezir ◽

Mina Hizal ◽

Burcu Cura Yayla ◽

Kubra Aykac ◽

Arzu Yilmaz ◽

...

Keyword(s):

Allergic Rhinitis ◽

Pediatric Patients ◽

Immunoglobulin E ◽

Allergic Diseases ◽

Clinical Severity ◽

Patients Âgés ◽

Skin Prick Tests ◽

Novel Coronavirus ◽

The Impact ◽

The Relationship

Background: There are conflicting data with regard to the impact of respiratory and allergic comorbidities on the course of novel coronavirus disease 2019 (COVID-19) in children. Objective: This study aimed to investigate the relationship between allergic diseases and COVID-19 severity in pediatric patients. Methods: Seventy-five pediatric patients with COVID-19 were classified according to clinical severity and evaluated in the allergy/immunology and pulmonology departments 1 to 3 months after the infection resolved. Blood was collected from the patients for a complete blood cell count and assessment of immunoglobulin and total immunoglobulin E (IgE) levels, and skin-prick tests and spirometry tests were performed. Results: A total of 75 patients ages 5‐18 years were evaluated. COVID-19 was asymptomatic/mild in 44 patients and moderate/severe/critical in 31 patients. Based on allergy evaluation, allergic rhinitis was diagnosed in 19 patients (25.3%), asthma in 10 patients (13%), and atopic dermatitis in 3 patients (4%). Aeroallergen sensitivity was detected in 26 patients (34.7%). COVID-19 infection was asymptomatic/mild in 15 patients with allergic rhinitis (78.9%) and in 21 with aeroallergen sensitivity (80.8%) (p = 0.038 and p = 0.005, respectively). There was no difference in severity between the patients with and without asthma (p = 0.550). The median (interquartile range) total IgE level was significantly higher in the asymptomatic/mild group (71.8 [30.7‐211.2]) (p = 0.015). There were no differences in terms of spirometry parameters. Conclusion: Aeroallergen sensitization and allergic rhinitis in children may be associated with a milder course of COVID-19. The knowledge that atopy is associated with less-severe COVID-19 outcomes in children may guide clinical risk classification.

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Elements of Immunoglobulin E Network Associate with Aortic Valve Area in Patients with Acquired Aortic Stenosis

Biomedicines ◽

10.3390/biomedicines9010023 ◽

2020 ◽

Vol 9 (1) ◽

pp. 23

Author(s):

Daniel P. Potaczek ◽

Aleksandra Przytulska-Szczerbik ◽

Stanisława Bazan-Socha ◽

Artur Jurczyszyn ◽

Ko Okumura ◽

...

Keyword(s):

Aortic Valve ◽

Immunoglobulin E ◽

Serum Levels ◽

Protective Role ◽

Total Serum ◽

Aortic Valve Area ◽

Protective Mechanisms ◽

Serum Ige ◽

High Affinity Receptor ◽

Valve Area

Allergic mechanisms are likely involved in atherosclerosis and its clinical presentations, such as coronary artery disease (CAD). It has been previously reported that CAD severity associates with serum levels of immunoglobulin E (IgE), the molecule that, along with its high-affinity receptor (FcԑRI), plays a central role in allergic reactions. Considering multiple pathophysiological similarities between atherosclerosis and acquired aortic (valve) stenosis (AS), we speculated that allergic pathways could also contribute to the AS mechanisms and grading. To validate this hypothesis, we first checked whether total serum IgE levels associate with echocardiographic markers of AS severity. Having found a positive correlation between serum IgE and aortic valve area (AVA), we further speculated that also total IgE-determining genetic polymorphisms in FCER1A, a locus encoding an allergen-biding FcԑRI subunit, are related to acquired AS severity. Indeed, the major allele of rs2251746 polymorphism, known to associate with higher IgE levels, turned out to correlate with larger AVA, a marker of less severe AS. Our findings surprisingly suggest a protective role of IgE pathways against AS progression. IgE-mediated protective mechanisms in AS require further investigations.

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Correlation of absolute eosinophil count, nasal smear eosinophilia and serum IgE levels in allergic rhinitis

IP Journal of Diagnostic Pathology and Oncology ◽

10.18231/j.jdpo.2021.061 ◽

2021 ◽

Vol 6 (4) ◽

pp. 287-290

Author(s):

Shruthi Gowthami M R

Keyword(s):

Allergic Rhinitis ◽

Eosinophil Count ◽

Simple Procedure ◽

Allergic Diseases ◽

Clinical Score ◽

Total Serum ◽

Common Disease ◽

Serum Ige ◽

Absolute Eosinophil Count ◽

One Year

Allergic rhinitis (AR) is one of the most common disease in India. The association between eosinophils and allergic diseases has been known since many years. To correlate Nasal smear eosinophilia (NSE), blood absolute eosinophil count (AEC) and total serum IgE levels with severity of the clinical score in patients with allergic rhinitis. This is one-year prospective study in patients (n=60) presented with allergic rhinitis to our institute. After taking consent, all the study participants were subjected for blood AEC, IgE levels and nasal smears for eosinophils. Among the samples, 81.7% were males. The mean age of patients was 35.2 years. AEC > 440 cells/cu.mm was seen in 46.6%, NSE>10% in 81.6% and total serum IgE was elevated > 100 IU/ml in 95.0%. There was a good correlation of NSE and serum IgE with the severity of disease. There was no correlation of blood AEC with the severity of clinical score. Data was analysed using SPSS software.rn NSE and IgE both correlated well with severity of the disease. Nasal smear cytology is a simple procedure having good correlation with severity of clinical score should be used routinely. Testing for serum IgE levels will increase the accuracy with a better diagnostic utility.

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Local Expression of Interleukin-17a is Correlated with Nasal Eosinophilia and Clinical Severity in Allergic Rhinitis

Allergy & Rhinology ◽

10.2500/ar.2014.5.0078 ◽

2014 ◽

Vol 5 (1) ◽

pp. ar.2014.5.0078 ◽

Cited By ~ 13

Author(s):

Seiichiro Makihara ◽

Mitsuhiro Okano ◽

Tazuko Fujiwara ◽

Yohei Noda ◽

Takaya Higaki ◽

...

Keyword(s):

Allergic Rhinitis ◽

Nasal Mucosa ◽

Immunoglobulin E ◽

Inferior Turbinate ◽

Forced Expiratory Volume ◽

Clinical Severity ◽

Eosinophil Infiltration ◽

Total Serum ◽

Blood Eosinophil ◽

Local Expression

Interleukin (IL)-17A is a major cytokine produced by Th17 cells, which are associated with chronic inflammations. The local expression of IL-17A in allergic rhinitis (AR) remains to be characterized. We sought to determine the role of IL-17A expression in human inferior turbinate mucosa in the pathophysiology of AR. Inferior turbinate mucosa was sampled from medical treatment-resistant, surgery-required patients with perennial AR (PAR, n = 21), nonallergic rhinitis with eosinophilia syndrome (NARES, n = 7), and nonallergic hypertrophic rhinitis (HR, n = 13). IL-17A expression was determined with immunohistochemical staining. The mean number of IL-17A+ cells and eosinophils per field were counted. Total serum immunoglobulin E (IgE) levels, blood eosinophil count, and forced expiratory volume in 1 second (FEV1)/forced vital capacity (FVC) ratio were also examined in each patient. IL-17A was primarily expressed in infiltrating inflammatory cells. The number of IL-17A+ cells in nasal mucosa was significantly higher in the PAR group compared with HR (p = 0.002) and NARES (p = 0.021) groups. There was a significant and positive correlation between the number of IL-17A+ cells and total nasal symptom score (rho = 0.403; p = 0.011), especially sneezing score (rho = 0.471; p = 0.003). The number of IL-17A+ cells was significantly and positively correlated with the degree of eosinophil infiltration (rho = 0.623; p < 0.001), but not with total serum IgE levels (rho = 0.284; p = 0.098), blood eosinophil counts (rho = 0.302; p = 0.056), or FEV1/FVC ratio (rho = 0.092; p = 0.569). The present study provides evidence that IL-17A expression in the nasal mucosa is associated with the pathophysiology of AR, including disease severity and nasal eosinophilia.

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Anti-inflammatory effect of wogonin on allergic responses in ovalbumin-induced allergic rhinitis in the mouse

Allergy & Rhinology ◽

10.1177/2152656718764145 ◽

2018 ◽

Vol 9 ◽

pp. 215265671876414 ◽

Cited By ~ 4

Author(s):

Kyeong Ah Kim ◽

Joo Hyun Jung ◽

Yun Sook Choi ◽

Gyu Kang ◽

Seon Tae Kim

Keyword(s):

Allergic Rhinitis ◽

Immunoglobulin E ◽

Allergic Diseases ◽

Serum Levels ◽

Lavage Fluid ◽

Specific Ige ◽

Eosinophil Infiltration ◽

Treatment Groups ◽

Specific Immunoglobulin E ◽

Group A

Background Wogonin is commonly used for the treatment of allergic diseases. However, neither its precise effect in preventing allergic rhinitis (AR) nor its mechanism of action are known. Objectives In this study, the effect of wogonin on allergic responses in ovalbumin (OVA) induced AR was investigated in mice. Methods BALB/c mice were sensitized with intraperitoneal (i.p.) OVA and then challenged intranasally with OVA. Wogonin (10 and 30 mg/kg) was given to the treatment groups, and the effect of wogonin on the release of allergic inflammatory mediators, specifically OVA-specific immunoglobulin E (IgE) and inflammatory cytokines, was explored. Eosinophil infiltration and the levels of interleukin (IL) 5 and IL-13 were measured by immunohistochemistry. Results In mice with AR, wogonin decreased OVA-specific IgE levels in serum, and the levels of the cytokines IL-4, IL-5, IL-13, eotaxin, and RANTES in nasal lavage fluid. Serum levels of IL-4, IL-5, and IL-13 were lower in both groups of wogonin-pretreated mice than in the OVA group. A reduction in eosinophil infiltration of the nasal mucosa and inhibition of the expression of IL-5 and IL-13 were also noted in the treated groups. Conclusion Wogonin induced antiallergic effects in a murine model of AR by decreasing the infiltration of eosinophils and levels of T-helper type 2 cytokines. Thus, wogonin merits consideration as a therapeutic agent for treating AR.

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Serum immunoglobulin E reactivity to cross-reacting panallergen components in north-eastern Poland patients pollen sensitized

Allergy and Asthma Proceedings ◽

10.2500/aap.2020.41.200002 ◽

2020 ◽

Vol 41 (3) ◽

pp. 183-191

Author(s):

Krzysztof Kowal ◽

Agnieszka Pampuch ◽

Ewa Sacharzewska ◽

Ewa Swiebocka ◽

Zenon Siergiejko ◽

...

Keyword(s):

Allergic Rhinitis ◽

Allergen Immunotherapy ◽

Immunoglobulin E ◽

Pollen Allergy ◽

Serum Ige ◽

Ige Reactivity ◽

Allergen Components ◽

North Eastern ◽

Allergen Sources ◽

Seasonal Pollen

Background: The presence of immunoglobulin E (IgE), which cross-reacts with allergen components, such as profilins, polcalcins, and cross-reacting carbohydrate determinants (CCD), creates a problem when selecting patients for allergen immunotherapy by using conventional methods. The aim of this study was to evaluate the prevalence of sensitization to profilins, polcalcins, and CCDs in patients with seasonal pollen allergic rhinitis. Methods: The study was performed on a group of 112 patients with seasonal pollen allergic rhinitis, ages 14 to 55 years, with sensitization to at least one seasonal allergen (IgE > 0.7 kUA/L). The presence of IgE sensitization to recombinant (r) Bet v 2, rPhl p 12, rBet v 4, rPhl p 7, and CCDs, in addition to rBet v 1, rPhl p 1, rPhl p 5, was evaluated by using a multiparameter immunoblot. Results: Among the studied patients, 64.3, 80.4, and 41.1% were sensitized to birch, timothy grass, and mugwort pollen, respectively. Sensitization to profilins rBet v 2/Phl p 12 was demonstrated in 28.6%, to polcalcins Bet v 4/Phl p 7 in 8.9%, and to CCDs in 25%. In 29.3%, serum IgE reactivity to any of the cross-reactive components could be demonstrated. Serum IgE reactivity to rBet v 2 was always accompanied by IgE reactivity to rPhl p 12, and IgE reactivity to rBet v 4 was always accompanied by IgE reactivity to rPhl p 7. Among the patients with pollinosis co-sensitized to at least two allergen sources according to extract-based diagnosis, possible false-positive results due to sensitization to cross-reactive components were detected in 17.9%. Conclusion: Evaluation of sensitization to cross-reacting components may be useful in evaluation of patients with pollen allergy who are being assessed for allergen immunotherapy to optimize the constitution of their immunotherapy vaccines.

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