scholarly journals Monthly and Yearly Variations in Oxygen Radical-scavenging Activity, Ascorbic Acid Content, and Degrees Brix of Komatsuna (Brassica rapa var. perviridis) in the Kanto Region, Japan

2020 ◽  
Vol 89 (3) ◽  
pp. 251-260
Author(s):  
Ayuchi Takei ◽  
Yuri Goh ◽  
Gen Hattori ◽  
Masayuki Arii ◽  
Masumi Niwa ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Brassica Rapa ◽  
Acid Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ascorbic Acid Content ◽  
Oxygen Radical ◽  
Scavenging Activity ◽  
Kanto Region

scholarly journals Screening of Chilli Pepper Genotypes as a Source of Capsaicinoids and Antioxidants under Conditions of Simulated Drought Stress

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 364 ◽  
Author(s):  
Tomas Kopta ◽  
Agnieszka Sekara ◽  
Robert Pokluda ◽  
Vojtech Ferby ◽  
Gianluca Caruso
Keyword(s):  
Ascorbic Acid ◽  
Drought Stress ◽  
Acid Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ascorbic Acid Content ◽  
Scavenging Activity ◽  
Vegetable Crops ◽  
Chilli Pepper ◽  
Heat Units

In many regions of the world, the production of vegetable crops is limited by a deepening water crisis. Drought stress affects productivity and the chemical composition of crops. The variability of drought tolerance between species and cultivars of economically important crops, such as pepper (Capsicum species), requires specific investigations to understand the physiological and biochemical responses to the aftermath of drought. The fruits and leaves of four chilli pepper cultivars were investigated to elucidate the fruits’ pungency (Scoville Heat Units, SHU), ascorbic acid content, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, polyphenol content, membrane lipid peroxidation and key protective antioxidant enzyme activity under drought stress (18–28% volumetric water content) as compared to the control (35–60%). Drought increased the chilli pepper fruits’ pungency expressed in Scoville Heat Units (SHU) as well as ascorbic acid content, but this relationship was also dependent on genotype and stress duration. ‘Jolokia’ was marked as most sensitive to drought by increasing content of capsaicinoids and DPPH˙ scavenging activity under stress conditions. Capsaicinoids and Ascorbic acid (AsA) greatly influenced the antioxidant activity of highly pungent chilli pepper fruits, although total phenols played a significant role in the mildly pungent genotypes. Generally, the activities of antioxidant enzymes increased under drought in chilli pepper leaves and fruits, although the intensity of the reaction varied among the cultivars used in the current research. All the investigated biochemical parameters were involved in the drought response of chilli pepper plants, but their significance and effectiveness were highly cultivar-dependent.

scholarly journals Biochemical, Phytochemical and Antioxidant Analysis of Leaves and Seed of Fenugreek Plant

2020 ◽  
Vol 1 (2) ◽  
pp. 9-18
Author(s):  
Haq Nawaz ◽  
Hina Andaleeb ◽  
Sobia Aziz ◽  
Saba Rehman
Keyword(s):  
Ascorbic Acid ◽  
Acid Content ◽  
Reducing Sugars ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ascorbic Acid Content ◽  
Dry Weight ◽  
Water Soluble ◽  
Total Phenolic

In the present study leaves and seeds of two different varieties of Fenugreek plant viz. Trigonella foenum gracium and Trigonella corniculata, collected from Bahawalpur, Pakistan were subjected to the biochemical, phytochemical and antioxidant analysis. The content of total sugars, non reducing sugars and reducing sugars in selected parts of these varities were found to be 5.007±0.101 to 8.133±0.223, 4.255±0.150 to 7.099±0.316 and 0.751±0.064 to 1.410±0.071 g/100g of dry weight respectively. Water soluble, salt soluble and total salt soluble protein content range from 1.172±0.001 to 6.015±0.132, 1.517±0.038 to 1.174±0.006 and 0.013±0.001 to 0.018±0.001 g/100 g of dry weight respectively and the total free amino acids ranged from 1.198±0.014 to 4.554±0.006 g/100 g of dry weight. The range of tannins and saponin contents in analyzed parts of these varities was found to be 0.057±0.009 to 0.117±0.025 and 0.083±0.012 to 0.0151±0.045 g /100g of dry weight respectively. Similarly, flavonoids and hydrogen cyanide content ranged from 0.021±0.000 to 0.055±0.004 and 0.0234±0.008 to 0.307±0.009 g/100g of dry weight respectively; while ascorbic acid content was found to be in the range of 0.068±0.08 to 0.267±0.031 g/100g of dry weight. Total phenolic acid content of the selected parts of these varities were estimated on the basis of difference in dipole moment and its range was observed from 0.0539±0.024 to 0.856±0.064 g/100g of dry weight. The total antioxidants analysis showed that ascorbic acid and trolox contents (mg Eqv./100g of extract) were found to be lower in Trigonella corniculata seeds (0.066±0.001 and 0.047±0.001 mg Eqv./100g respectively) and higher in Trigonella corniculata leaves (0.069±0.001 and 0.050±0.001 mg Eqv./100g respectively).The DPPH radical scavenging activity and reducing power were found to be in the range of 31.736±2.430 to 39.590±2.432 and 0.974±0.231 to 2.301±0.068 respectively. The different parts of studied varities of fenugreek plant, having higher values of %DPPH inhibition and reducing power showed least count of IC50 and vice versa.

scholarly journals Carotenoids, sugars, ascorbic acid, total phenolics, and antioxidant activity of murici from Brazilian Cerrado during refrigerated storage

2020 ◽  
Vol 50 (4) ◽  
Author(s):  
Celso Martins Belisário ◽  
Antônio Gomes Soares ◽  
Regina Celi Cavestré Coneglian ◽  
Geovana Rocha Plácido ◽  
Carlos Frederico de Souza Castro ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Acid Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Storage Period ◽  
Ascorbic Acid Content ◽  
High Concentration ◽  
Total Carotenoids ◽  
Byrsonima Crassifolia ◽  
Small Producers

ABSTRACT: Native fruits are economically important to small producers, and they are a important part of the diet of several communities. Therefore, postharvest studies of these fruits are essential. In addition, research involving their chemical composition can identify substances that add potential value to the fruits, especially from a nutritional and medicinal standpoint. This study characterized the fruits of the muricizeiro shrub (Byrsonima crassifolia, Malpighiaceae), which were harvested from native plants on private properties and stored for 16 days at a mean temperature of 12 °C. The fruits were evaluated during storage for: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ascorbic acid content, phenolics and carotenoids total, carotenoids profile, glucose, fructose and sucrose contents. Overall, the temperature that the fruits were storage at was effective at maintaining the quality of the fruit. However, the ascorbic acid content of the fruits did decrease during the storage period. Results showed that the fruits had a high antioxidant capacity, possibly because of the presence of phenolic compounds and carotenoids. It is also important to highlight that this fruit is source of vitamin A, owing to the high concentration of β-carotene.

Synthesis and Biological Activities of Hydroxytyrosol Ester Derivatives

2020 ◽  
Vol 42 (1) ◽  
pp. 109-109
Author(s):  
Hao Zang Hao Zang ◽  
Qian Xu Qian Xu ◽  
Luyun Zhang Luyun Zhang ◽  
Guangqing Xia Guangqing Xia ◽  
Jiaming Sun and Junyi Zhu Jiaming Sun and Junyi Zhu
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Acetylcholinesterase Inhibition ◽  
Hydroxyl Group ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Better Than

A series of hydroxytyrosol (HT) derivatives were synthesized by modification of alcohol hydroxyl group of HT, twenty-five target compounds were obtained and characterized by NMR and HRMS. The antioxidant activities of those compounds were evaluated in three different assays. Except 3e and 3y, all other compounds demonstrated significant 2,2and#39;-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radical cation scavenging activity ranging from IC50 3.4 to 24.4 μM, which were more potent than L-ascorbic acid (IC50=24.8 μM). Compounds 3b-3d, 3f-3k, 3m-3x were better than Trolox (18.3 M). Moreover, the ferric reducing antioxidant power (FRAP) of all compounds were discovered to be more potent than L-ascorbic acid (40.7 mmol/g), except 3e, all other compounds (141.5-202.1 mmol/g) were better than Trolox (94.7 mmol/g). Compounds 3a-3d, 3f-3j, 3l-3m, 3o, 3q, 3t, 3v-3y exhibited more potent hydroxyl radical scavenging activity (IC50=245.1-475.1 M) than L-ascorbic acid (554.4 M) and Trolox (500.4 M). Compounds 3q, 3t and 3y exhibited more potent -Glucosidase inhibition activity (39.1-52.4 M) than Acarbose (60.9 M). Compounds 3a, 3d, 3f-3m, 3s-3t, 3v-3y showed some acetylcholinesterase inhibition activities, compounds 3a, 3d, 3f-3j, 3l-3m, 3o-3p, 3s-3t, 3w showed some butyrylcholinesterase inhibition activities.

scholarly journals The Antioxidant and Antiproliferative Activities of 1,2,3-Triazolyl-L-Ascorbic Acid Derivatives

2019 ◽  
Vol 20 (19) ◽  
pp. 4735 ◽  
Author(s):  
Anja Harej ◽  
Andrijana Meščić Macan ◽  
Višnja Stepanić ◽  
Marko Klobučar ◽  
Krešimir Pavelić ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Hydrogen Atom Transfer ◽  
Breast Adenocarcinoma ◽  
Scavenging Activity ◽  
Activity Data ◽  
Oh Groups ◽  
Mcf 7

The novel 4-substituted 1,2,3-triazole L-ascorbic acid (L-ASA) conjugates with hydroxyethylene spacer as well as their conformationally restricted 4,5-unsaturated analogues were synthesized as potential antioxidant and antiproliferative agents. An evaluation of the antioxidant activity of novel compounds showed that the majority of the 4,5-unsaturated L-ASA derivatives showed a better antioxidant activity compared to their saturated counterparts. m-Hydroxyphenyl (7j), p-pentylphenyl (7k) and 2-hydroxyethyl (7q) substituted 4,5-unsaturated 1,2,3-triazole L-ASA derivatives exhibited very efficient and rapid (within 5 min) 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging activity (7j, 7k: IC50 = 0.06 mM; 7q: IC50 = 0.07 mM). In vitro scavenging activity data were supported by in silico quantum-chemical modelling. Thermodynamic parameters for hydrogen-atom transfer and electron-transfer radical scavenging pathways of anions deprotonated at C2-OH or C3-OH groups of L-ASA fragments were calculated. The structure activity analysis (SAR) through principal component analysis indicated radical scavenging activity by the participation of OH group with favorable reaction parameters: the C3-OH group of saturated C4-C5(OH) derivatives and the C2-OH group of their unsaturated C4=C5 analogues. The antiproliferative evaluation showed that p-bromophenyl (4e: IC50 = 6.72 μM) and p-pentylphenyl-substituted 1,2,3-triazole L-ASA conjugate (4k: IC50 = 26.91 μM) had a selective cytotoxic effect on breast adenocarcinoma MCF-7 cells. Moreover, compound 4e did not inhibit the growth of foreskin fibroblasts (IC50 > 100 μM). In MCF-7 cells treated with 4e, a significant increase of hydroxylated hypoxia-inducible transcription factor 1 alpha (HIF-1α) expression and decreased expression of nitric oxide synthase 2 (NOS2) were observed, suggesting the involvement of 4e in the HIF-1α signaling pathway for its strong growth-inhibition effect on MCF-7 cells.

scholarly journals New 1,3,5-Triazine Derivatives Incorporating Aminobenzenesulfonamide, Aminoalcohol, Piperazine, Chalcone or Stilbene Structural Motifs and Evaluation of Their Antioxidative Activity

Proceedings ◽  
2019 ◽  
Vol 41 (1) ◽  
pp. 17
Author(s):  
Eva Havránková ◽  
Nikola Čalkovská ◽  
Tereza Padrtová ◽  
Jozef Csöllei ◽  
Radka Opatřilová ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Antioxidative Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Inhibition Activity ◽  
Structural Motifs ◽  
Triazine Derivatives ◽  
Acidic Resin

A series of 1,3,5-triazine derivatives, incorporating aminobenzenesulfonamide, aminoalcohol/phenol, piperazine, chalcone, or stilbene structural motifs, were evaluated as potential antioxidants. The compounds were prepared by using step by step nucleophilic substitution of chlorine atoms in starting 2,4,6-trichloro-1,3,5-triazine. Reactions were catalyzed by Cu(I)-supported on a weakly acidic resin. The radical scavenging activity was determined in terms of %inhibition activity and IC50, using the ABTS method. Trolox and ascorbic acid (ASA) were used as standards. In the lowest used concentration 1 × 10−4 M, the %inhibition activity at time 0 min was comparable with both standards at least for 10 compounds. After 60 min compounds 1, 2, 9 and 25 showed nearly twice %inhibition (73.44–87.09%) in comparison with standards (Trolox = 41.49%; ASA = 31.07%). Values of IC50 correlated with %inhibition activity. For compounds 1, 2, 9 and 25 values of IC50 in time 60 min (17.16–27.78 μM) were 5 times lower than IC50 of both standards (Trolox = 178.33 μM; ASA = 147.47 μM). Based on these results, the presented 1,3,5-triazine derivatives and their analogs have a high potential in the treatment of illnesses caused or related to oxidative stress.

scholarly journals Inhibition of LDL oxidation by melatonin requires supraphysiologic concentrations

1998 ◽  
Vol 44 (9) ◽  
pp. 1931-1936 ◽  
Author(s):  
P Barton Duell ◽  
David L Wheaton ◽  
Amy Shultz ◽  
Hien Nguyen
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Metal Ion ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ldl Oxidation ◽  
Human Macrophage ◽  
Published Data ◽  
Scavenging Activity ◽  
Mediated Oxidation

Abstract Melatonin has been suggested as a potent antioxidant that may protect against development of atherosclerosis and cancer; however, these effects are unproven and controversial. The antioxidant capacity of melatonin was tested in comparison with α-tocopherol, ascorbic acid, and the melatonin precursors tryptophan and serotonin, by measuring inhibition of metal ion-mediated and human macrophage-mediated oxidation of LDL. Melatonin had weak antioxidant activity that was detectable only at concentrations 10 000- to 100 000-fold higher than physiologic concentrations. These results were comparable with published data showing that the radical scavenging activity of melatonin requires markedly supraphysiologic concentrations. In contrast, α-tocopherol was 50- to 100-fold more potent and was efficacious at physiologic concentrations. Ascorbic acid and tryptophan also were active at physiologic concentrations and were significantly more potent than melatonin. In summary, extremely supraphysiologic concentrations of melatonin had only weak antioxidant activity, which was surpassed by α-tocopherol, ascorbic acid, and tryptophan.

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