scholarly journals 79. Morphological Observation of Candida albicans-Chlamydospore Formation on SCLT : Corn Meal Agar and Chlamydoscope Agar Media

1967 ◽  
Vol 21 (3) ◽  
pp. 300-302
Author(s):  
H. Matsumoto
Keyword(s):  
Candida Albicans ◽  
Morphological Observation ◽  
Corn Meal ◽  
Corn Meal Agar ◽  
Chlamydospore Formation ◽  
Agar Media

In vivo synthesis of sulfur containing amino acids in Candida albicans

1973 ◽  
Vol 19 (2) ◽  
pp. 155-161 ◽  
Author(s):  
David N. Mardon
Keyword(s):  
Amino Acids ◽  
Candida Albicans ◽  
Corn Meal ◽  
Corn Meal Agar ◽  
Methionine Biosynthesis ◽  
Chlamydospore Formation ◽  
The Media ◽  
Cysteine Synthetase ◽  
Sulfur Containing

Growth in a supplemented minimal culture medium and chlamydospore formation in supplemented corn meal agar were the criteria used to evaluate in vivo biosynthesis of sulfur containing amino acids in auxotrophs of Candida albicans. Supplementation with L-cystathionine, L-homocysteine, L-methionine, S-adenosyl-L-methionine, or S-adenosyl-L-homocysteine resulted in growth in the minimal medium and both growth and chlamydospore formation in corn meal agar. One auxotroph (CYS) gave comparable results when inoculated into media supplemented with L-cysteine, responded minimally when O-acetyl-L-serine and sulfide comprised a dual supplement, and failed to grow or form chlamydospores when sulfide or O-acetyl-L-serine was used as a single supplement, suggesting that this organism may possess a deficiency in the synthesis or activity or cysteine synthetase. Another auxotroph (OAHS) did not grow or produce chlamydospores when O-acetyl-L-serine was added to the media in combination with sulfide, or when either L-cysteine, O-acetyl-L-serine, sulfide, or L-homoserine was used as a single supplement, but did respond to media supplemented with O-acetyl-L-homoserine indicating a deficiency in homoserine-O-transacetylase in this microorganism. Auxotroph OAHS grew and formed chlamydospores as well in media containing only supplemental O-acetyl-L-homoserine as it did when O-acetyl-L-homoserine was used in combination with sulfide S-methyl-L-cysteine or thiomethyladenosine, while (CYS) neither grew nor produced chlamydospores in any of these supplemented media. These results lend support for the hypothesis that cystathionine is an important intermediate in methionine biosynthesis in C. albicans. The positive results obtained when the auxotrophs were inoculated into media supplemented with S-adenosyl-L-homocysteine or S-adenosyl-L-methionine may reflect an alternate route for methionine biosynthesis which functions conditionally in this microorganism. The potential significance for endogenous formation of sulfur containing amino acids from S-adenosylmethionine is therefore considered.

scholarly journals The Cek1 and Hog1 Mitogen-Activated Protein Kinases Play Complementary Roles in Cell Wall Biogenesis and Chlamydospore Formation in the Fungal Pathogen Candida albicans

2006 ◽  
Vol 5 (2) ◽  
pp. 347-358 ◽  
Author(s):  
B. Eisman ◽  
R. Alonso-Monge ◽  
E. Román ◽  
D. Arana ◽  
C. Nombela ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Map Kinase ◽  
Fungal Pathogen ◽  
Hog Pathway ◽  
Cell Wall Biogenesis ◽  
Chlamydospore Formation ◽  
Mitogen Activated Protein ◽  
Morphological Transitions ◽  
Relationship Of

ABSTRACT The Hog1 mitogen-activated protein (MAP) kinase mediates an adaptive response to both osmotic and oxidative stress in the fungal pathogen Candida albicans. This protein also participates in two distinct morphogenetic processes, namely the yeast-to-hypha transition (as a repressor) and chlamydospore formation (as an inducer). We show here that repression of filamentous growth occurs both under serum limitation and under other partially inducing conditions, such as low temperature, low pH, or nitrogen starvation. To understand the relationship of the HOG pathway to other MAP kinase cascades that also play a role in morphological transitions, we have constructed and characterized a set of double mutants in which we deleted both the HOG1 gene and other signaling elements (the CST20, CLA4, and HST7 kinases, the CPH1 and EFG1 transcription factors, and the CPP1 protein phosphatase). We also show that Hog1 prevents the yeast-to-hypha switch independent of all the elements analyzed and that the inability of the hog1 mutants to form chlamydospores is suppressed when additional elements of the CEK1 pathway (CST20 or HST7) are altered. Finally, we report that Hog1 represses the activation of the Cek1 MAP kinase under basal conditions and that Cek1 activation correlates with resistance to certain cell wall inhibitors (such as Congo red), demonstrating a role for this pathway in cell wall biogenesis.

scholarly journals Effect of the metalic salts on the pseudohyphae and chlamydospore formation of Candida albicans

1973 ◽  
Vol 14 (2) ◽  
pp. 106-108_1
Author(s):  
Tomi TAMAMURA ◽  
Teruo ISODA ◽  
Fukumitsu YAMAGUCHI ◽  
Yoshio SCHÖBL
Keyword(s):  
Candida Albicans ◽  
Chlamydospore Formation

scholarly journals Candida albicans isolated from post-operative penetrating keratoplasty patient: Case report

2014 ◽  
Vol 5 (3) ◽  
pp. 116-119
Author(s):  
Abhishek Chandra ◽  
Munesh Kumar Gupta ◽  
Ragini Tilak
Keyword(s):  
Candida Albicans ◽  
Case Report ◽  
Amphotericin B ◽  
Penetrating Keratoplasty ◽  
Antifungal Susceptibility ◽  
Medical Science ◽  
Eye Drops ◽  
Gram Staining ◽  
Chlamydospore Formation ◽  
Culture Characteristics

We report a case report of Candida albicans suture infiltrate on 3rd post-op day in a 53 year female operated for penetrating keratoplasty. Candida albicans was identified by KOH mount, Gram Staining, germ tube, growth at 450C, chlamydospore formation and light green color on CHROMagar with sugar assimilation and culture characteristics. Despite being susceptible to Fluconazole by broth microdilution, patient did not respond to 0.3% fluconazole eye drops. On antifungal susceptibility testing by CLSI44A, it was susceptible to only Amphotericin B (100units). Patient was then started on 0.15% fortified amphotericin B eye drops resulting in complete resolution of infiltrates. Asian Journal of Medical Science, Volume-5(3) 2014: 116-119 http://dx.doi.org/10.3126/ajms.v5i3.8669 

A simple liquid medium for chlamydospore formation in Candida albicans

1978 ◽  
Vol 64 (3) ◽  
pp. 143-146 ◽  
Author(s):  
M. Gunasekaran ◽  
Walter T. Hughes
Keyword(s):  
Candida Albicans ◽  
Liquid Medium ◽  
Simple Liquid ◽  
Chlamydospore Formation

DURANDIELLA PSEUDOTSUGAE N. SP.; TAXONOMY, CULTURAL CHARACTERISTICS, LIFE HISTORY, AND HOST RESPONSE

1962 ◽  
Vol 40 (2) ◽  
pp. 331-335 ◽  
Author(s):  
A. Funk
Keyword(s):  
Life History ◽  
New Species ◽  
Host Response ◽  
Maximum Growth ◽  
Perfect State ◽  
Corn Meal ◽  
Corn Meal Agar ◽  
Cultural Characteristics ◽  
Infected Area ◽  
A New Species

The perfect state of Chondropodium pseudotsugae White is shown to be a new species of Durandiella, for which the name D. pseudotsugae is proposed. Maximum growth in culture is obtained on corn meal agar at 15 °C. Apothecia are produced in the spring; the ascospores are forcibly discharged; pycnidia persist throughout the summer and discharge spores only when moistened. The host response of Douglas fir is the production of a "button" of persistent secondary periderm beneath the infected area.

Resistance of chlamydospores of nematophagous fungi to the digestive processes of sheep in Mexico

1998 ◽  
Vol 72 (2) ◽  
pp. 155-158 ◽  
Author(s):  
R.D. Llerandi-Juárez ◽  
P. Mendoza-de Gives
Keyword(s):  
Oral Administration ◽  
Haemonchus Contortus ◽  
Corn Meal ◽  
Corn Meal Agar ◽  
Duddingtonia Flagrans ◽  
Free Living ◽  
Oral Inoculation ◽  
Third Stage ◽  
Mexican Isolate ◽  
Digestive Processes

AbstractFollowing oral administration to sheep, chlamydospores of a Mexican isolate of Duddingtonia flagrans (FTHO-8) survived passage through the digestive tract and subsequently grew on corn meal agar plates. The fungus was able to catch and destroy free-living nematodes and third-stage larvae of Haemonchus contortus added to these plates. Chlamydospores of Arthrobotrys sp. showed a poor resistance to the digestive processes of sheep, although conidia of A. superba survived following oral inoculation in one of two animals.

Antibacterial Efficacy of the Compound Preparation of Traditional Chinese Drug on System Fungi Reside in Faecal Specimen of Pigeon

2012 ◽  
Vol 518-523 ◽  
pp. 5514-5517 ◽  
Author(s):  
Lian Lan Ma ◽  
Zhi Chun Liu ◽  
Xiao Yuan Wang ◽  
Xiao Yun Wu
Keyword(s):  
Candida Albicans ◽  
Cryptococcus Neoformans ◽  
Drug Concentration ◽  
Chinese Drug ◽  
Faecal Specimen ◽  
Sterile Saline ◽  
Gram Staining ◽  
Chlamydospore Formation ◽  
Positive Rate ◽  
Acid Test

To investigate the efficacy of the compound preparation of traditional Chinese drug which is named KZY-2) on system fungi reside in faecal specimen of pigeon, the 66 faecal specimen of pigeon were collected from columbary of local park and residents.Each specimen which weigh 2.0g was mixed with 10ml sterile saline and the supernatant which volume was 1ml was incubated with the same volume drug which concentration were 200mɡ/ml,100mɡ/ml and 50mɡ/ml respectively at 37°C.After 24h,48h and 72h, the isolated positive rates of Cryptococcus neoformans and Candida albicans were calculated. Candida albicans was identified by gram staining, budding development, chlamydospore formation, sugar fermented test,sugar assimilated test and Cryptococcus neoformans was identified by gram staining,sugar fermented test,sugar assimilated test,urease tests,caffeic acid test, Hiss capsule staining.Results showed that the isolated positive rates of Cryptococcus neoformans and Candida albicans were 27.3%（18/66） and 16.7%（11/66） without drug action, KZY-2 could reduce the isolated positive rates of Cryptococcus neoformans and Candida albicans(P<0.05 and P<0.05).The drug concentration and action time could significantly affect the isolated positive rate which was negative correlation between them, higher drug concentration and longer incubation time,more lower the positive rate, especially the isolated positive rates of Cryptococcus neoformans are reduced 77.7%（6.1/27.3）which incubated with 200mɡ/ml drug after 72h compared with the control group.These results indicate The KZY-2 have good germicidal efficacy on Cryptococcus neoformans and Candida albicans isolated from faecal specimen of pigeon.

AMIDOMYCIN, A NEW ANTIBIOTIC FROM A STREPTOMYCES SPECIES. PRODUCTION, ISOLATION, ASSAY, AND BIOLOGICAL PROPERTIES

1957 ◽  
Vol 3 (7) ◽  
pp. 953-965 ◽  
Author(s):  
W. A. Taber ◽  
L. C. Vining
Keyword(s):  
Candida Albicans ◽  
Plant Pathogens ◽  
Degradation Products ◽  
Biological Properties ◽  
Puccinia Graminis ◽  
Serial Transfer ◽  
Streptomyces Species ◽  
Low Concentrations ◽  
Agar Media ◽  
Species Production

Antifungal preparations were obtained from cultures of Streplomyces PRL 1642 by solvent extraction of the solids collected after the pH was adjusted to 3.5. The active factor, named amidomycin, was purified by repeated crystallization from aqueous ethanol or petrol (b.p. 60°–80 °C.) to give stable, colorless, optically active needles, m.p. 192 °C.Amidomycin suspended in agar media retarded the growth of many filamentous fungi and noticeably inhibited the plant pathogens Ustilago maydis, Ustilago trebouxii, as well as the human pathogen, Hormodendrum pedrosoi. It also inhibited the germination of uredospores of Puccinia graminis at low concentrations. Certain yeasts were completely inhibited by small concentrations of amidomycin; the quantity required was affected by the number of cells in the inoculum.None of the bacteria examined was inhibited by this antibiotic. At certain concentrations it was lethal to Candida albicans as determined by the inability of previously exposed and washed cells to grow on nutrient agar.Although a few isolated colonies of Candida albicans usually developed on plates containing approximately double the concentration required to inhibit growth of the inoculum streak, serial transfer of progeny from such colonies onto agar containing amidomycin did not produce cultures having progressively increasing resistance.Two degradation products of amidomycin, D(–)-valine and 3,6-diisopropyl-2,5-diketomorpholine, are inactive.

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