scholarly journals Acute Toxicity of Cigarette from Vietnam on in vivo Model Zebrafish (Danio rerio) Larvae

2021 ◽  
Vol 37 (4) ◽  
Author(s):  
Kieu Kien Trung ◽  
Nguyen Thi Huyen Trang ◽  
Vu Thi Hien ◽  
Nguyen Lai Thanh
Keyword(s):  
Acute Toxicity ◽  
Danio Rerio ◽  
Classification Criteria ◽  
In Vivo Model ◽  
Health Issues ◽  
Tobacco Products ◽  
Zebrafish Larvae ◽  
Particle Matter ◽  
Total Particle

Smoking is widely known to has a major contributor to public health issues both in worldwide and Vietnam. At the same time, Vietnam have a large number of smokers and the market is filled with diverse brand of tobacco products. In this study, we sampled 6 types of cigarette: ThangLong, Vina, Craven, Demi, Maxx and Bastos from local stores and conducted toxicological test on zebrafish larvae. Our results show that there are varied in toxicological properties of total particle matter collected from different cigarette brands in lethal and morphology effects on zebrafish embryos. LC50 of 6 brands after 96 hours of exposure were: ThangLong = 48.7 mg/L, Vina = 45.96 mg/L, Craven = 80.52 mg/L, Demi = 30.91 mg/L, Maxx = 83.54 mg/L and Bastos = 74.92 mg/L. They are lower than 100 mg/L, which put them under Category 2 and 3 in the GHS classification criteria for acute toxicity.

scholarly journals A White Grape Juice Extract Reduces Fat Accumulation through the Modulation of Ghrelin and Leptin Expression in an In Vivo Model of Overfed Zebrafish

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1119
Author(s):  
Giuseppe Montalbano ◽  
Alessandro Maugeri ◽  
Maria Cristina Guerrera ◽  
Natalizia Miceli ◽  
Michele Navarra ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Potential Role ◽  
Sedentary Lifestyle ◽  
Grape Juice ◽  
In Vivo Model ◽  
Gene Level ◽  
Gene Expression Analyses ◽  
Problematic Issue ◽  
Leptin Expression

A caloric surplus and a sedentary lifestyle are undoubtedly known to be the leading causes of obesity. Natural products represent valuable allies to face this problematic issue. This study was planned to assess the effect of a white grape (Vitis vinifera) juice extract (WGJe) in diet-induced obese zebrafish (Danio rerio). Fish were divided into four different diet groups: (i) normally fed (NF); (ii) overfed (OF); (iii) WGJe-supplemented NF (5 mL/L in fish water); (iv) WGJe-supplemented OF. Body mass index (BMI) was extrapolated each week. After the fourth week, euthanized zebrafish were processed for both microscopic evaluations and gene expression analyses. OF zebrafish showed higher BMI values with respect to NF counterparts, an effect that was hindered by WGJe treatment. Moreover, histological analyses showed that the area of the adipose tissue, as well as the number, size, and density of adipocytes was significantly higher in OF fish. On the other hand, WGJe was able to avoid these outcomes both at the subcutaneous and visceral levels, albeit to different extents. At the gene level, WGJe restored the altered levels of ghrelin and leptin of OF fish both in gut and brain. Overall, our results support the anti-obesity property of WGJe, suggesting its potential role in weight management.

scholarly journals HGG-23. IN VITRO AND IN VIVO PRECLINICAL DRUG SCREENING OF PROMISING THERAPEUTICS FOR DIFFUSE MIDLINE GLIOMA (DMG)

2021 ◽  
Vol 23 (Supplement_1) ◽  
pp. i21-i22
Author(s):  
Chiara Cianciolo Cosentino ◽  
Sandra Laternser ◽  
Justyna M Przystal ◽  
Sridevi Yadavilli ◽  
Jie Zhang ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Tumor Biology ◽  
In Vivo Studies ◽  
Continuous Exposure ◽  
Intrinsic Resistance ◽  
Drug Candidates ◽  
Zebrafish Larvae ◽  
Wide Range

Abstract Introduction Diffuse midline gliomas (DMGs) are amongst the most unforgiving pediatric brain tumors, characterized by an intrinsic resistance to therapy. Despite major advances in understanding of tumor biology, the prognosis remains exceedingly poor, and treatment options are limited. New therapeutics are being evaluated at a fast rate by different laboratories. In order to prioritize effective drug candidates for DMG treatment, we comprehensively characterized a panel of promising therapeutic agents in in vitro and in different vivo systems. Methods We determined the sensitivity of primary DMG cell lines to a panel of small molecule inhibitors targeting known DMG targets and pathways. Dose response curves were generated for more than 20 different compounds and possible synergistic effects were investigated by SynergieFinder. In an effort to highlight potential toxicities and associated mechanisms at a large scale, we performed a preclinical toxicity evaluation in zebrafish larvae, with a slightly modified version of the official Fish Embryo Acute Toxicity (FET) test. Drug toxicity was tested by continuous exposure of zebrafish larvae to increasing concentrations of the different compounds. Survival curves, morphological analyses and behavioral tests were performed at a maximum tolerated dose (MTD). To confirm the findings obtained in zebrafish, we further performed in vivo studies in mice for promising candidates. Results Among the tested drugs in vitro we found 10 drugs showing promising dose- dependent reduction in cell viability with IC50 in nM to µM range. These were further evaluated for toxicity in zebrafish. The zebrafish larvae toxicities observations strongly correlated with the findings in murine in vivo studies, reinforcing the importance of zebrafish as an accurate investigative toxicology model to assess acute toxicity of molecules in preclinical studies. Conclusions By testing a wide range of drugs, targeting different pathways on DMG cells and in different in vivo systems we identified promising drug candidates for clinical management of children diagnosed with DMG.

A transgenic zebrafish model of neutrophilic inflammation

Blood ◽  
2006 ◽  
Vol 108 (13) ◽  
pp. 3976-3978 ◽  
Author(s):  
Stephen A. Renshaw ◽  
Catherine A. Loynes ◽  
Daniel M.I. Trushell ◽  
Stone Elworthy ◽  
Philip W. Ingham ◽  
...  
Keyword(s):  
Time Course ◽  
Digital Image Analysis ◽  
Experimental Manipulation ◽  
In Vivo Model ◽  
Transgenic Zebrafish ◽  
Zebrafish Model ◽  
Similar Time ◽  
Zebrafish Larvae ◽  
Fluorescent Cells

Abstract We have established an in vivo model for genetic analysis of the inflammatory response by generating a transgenic zebrafish line that expresses GFP under the neutrophil-specific myeloperoxidase promoter. We show that inflammation is induced after transection of the tail of zebrafish larvae and that this inflammation subsequently resolves over a similar time course to mammalian systems. Quantitative data can be generated from this model by counting of fluorescent cells or by digital image analysis. In addition, we show that the resolution of experimentally induced inflammation can be inhibited by the addition of a pancaspase inhibitor, zVD.fmk, demonstrating that experimental manipulation of the resolution of inflammation is possible in this model.

A study on acute toxicity and solvent capacity of solvate ionic liquids in vivo using a zebrafish model (Danio rerio)

2016 ◽  
Vol 40 (8) ◽  
pp. 6599-6603 ◽  
Author(s):  
Prusothman Yoganantharajah ◽  
Daniel J. Eyckens ◽  
Jessie L. Pedrina ◽  
Luke C. Henderson ◽  
Yann Gibert
Keyword(s):  
Ionic Liquids ◽  
Acute Toxicity ◽  
Danio Rerio ◽  
Zebrafish Model

The in vivo toxicity of several solvate ionic liquids have been assessed using a zebrafish model.

scholarly journals In Vivo Evaluation of Cannabis sativa Full Extract on Zebrafish Larvae Development, Locomotion Behavior and Gene Expression

2021 ◽  
Vol 14 (12) ◽  
pp. 1224
Author(s):  
Rosario Licitra ◽  
Marco Martinelli ◽  
Luigi Petrocchi Jasinski ◽  
Maria Marchese ◽  
Claudia Kiferle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cannabinoid Receptors ◽  
Cannabis Sativa ◽  
Endocannabinoid System ◽  
In Vivo Model ◽  
Pcr Analysis ◽  
In Vivo Evaluation ◽  
Zebrafish Larvae ◽  
Locomotion Behavior

Historically, humans have been using Cannabis sativa for both recreational and medical purposes. Nowadays, cannabis-based products have gained scientific interest due to their beneficial effects on several syndromes and illnesses. The biological activity of cannabinoids is essentially due to the interaction with the endocannabinoid system, and zebrafish (Danio rerio) is a very well-known and powerful in vivo model for studying such specific interactions. The aim of the study was to investigate the effects of different doses of a Cannabis sativa whole extract [dissolved in dimethyl sulfoxide (DMSO)] on zebrafish eggs’ hatchability, embryo post-hatching survival, larvae locomotion behavior and mRNA gene expression. The results showed the absence of toxicity, and no significant differences were observed between treatments for both embryo hatching and survival rate. In addition, larvae exposed to the cannabis extract at the highest dose [containing 1.73 nM and 22.3 nM of ∆9-tetrahydrocannabinol (THC) and cannabidiol (CBD), respectively] showed an increased locomotion compared to the control and DMSO treated groups. Moreover, qRT-PCR analysis showed that the highest dosage of cannabis induced an over-expression of cnr1 and cnr2 cannabinoid receptors. In conclusion, the exposition of zebrafish larvae to the whole extract of Cannabis sativa showed no negative effects on embryo development and survival and enhanced the larvae’s locomotor performances. These findings may open up possible Cannabis sativa applications in human pharmacology as well as in other animal sectors.

Acute Toxicity of Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Ethanol Extract on Zebrafish (Danio rerio) Embryo In Vivo

2020 ◽  
Author(s):  
Fajar Fakri ◽  
Loly Subhiaty Idrus ◽  
Maria Alexandra Iskandar ◽  
Indra Wibowo ◽  
I Ketut Adnyana
Keyword(s):  
Acute Toxicity ◽  
Danio Rerio ◽  
Toxicity Testing ◽  
Ethanol Extract ◽  
Embryo Morphology ◽  
Zebrafish Embryos ◽  
Zebrafish Model ◽  
Pericardial Edema ◽  
High Concentrations

Keladi tikus (Typhonium flagelliforme (Lodd.) Blume) is an Indonesian medicinal plant that has various pharmacological properties. Zebrafish (Danio rerio) has been proposed as a model that can bridge the gap between cell assays and rodent assays. Evaluation of the toxic effects of natural products using the Zebrafish model can be assessed starting from the blastula stage of embryonic development. This study aims to investigate the potential acute toxicity effect of keladi tikus-ethanol extract (KTEE) using zebrafish embryos. A static non-replacement regime for acute toxicity testing was used. Wild-type zebrafish embryos were exposed to various concentrations of KTEE (50, 100, 200, 400, 800, 1600 µg/mL) starting at 6 hours post-fertilization (hpf) until 96 hpf. The results showed that the survival rate of zebrafish embryos decreased as the concentration of the test extract increased. The LC50 values of KTEE were 494.553 µg/mL at 96 hpf and 555.787 µg/mL at 72 hpf. Embryotoxicity effect of KTEE includes hatching delays and decreased heartrate on zebrafish embryos, especially at high concentrations. KTEE also caused abnormalities in embryo morphology, including pericardial edema, jaw and tail deformity.

scholarly journals Single-cell functional and chemosensitive profiling of combinatorial colorectal therapy in zebrafish xenografts

2017 ◽  
Vol 114 (39) ◽  
pp. E8234-E8243 ◽  
Author(s):  
Rita Fior ◽  
Vanda Póvoa ◽  
Raquel V. Mendes ◽  
Tânia Carvalho ◽  
António Gomes ◽  
...  
Keyword(s):  
Folinic Acid ◽  
Therapy Response ◽  
In Vivo Model ◽  
Cancer Heterogeneity ◽  
Zebrafish Larvae ◽  
Response To Chemotherapy ◽  
Therapeutic Guidelines ◽  
Highly Sensitive ◽  
A Minor

Cancer is as unique as the person fighting it. With the exception of a few biomarker-driven therapies, patients go through rounds of trial-and-error approaches to find the best treatment. Using patient-derived cell lines, we show that zebrafish larvae xenotransplants constitute a fast and highly sensitive in vivo model for differential therapy response, with resolution to reveal intratumor functional cancer heterogeneity. We screened international colorectal cancer therapeutic guidelines and determined distinct functional tumor behaviors (proliferation, metastasis, and angiogenesis) and differential sensitivities to standard therapy. We observed a general higher sensitivity to FOLFIRI [5-fluorouracil(FU)+irinotecan+folinic acid] than to FOLFOX (5-FU+oxaliplatin+folinic acid), not only between isogenic tumors but also within the same tumor. We directly compared zebrafish xenografts with mouse xenografts and show that relative sensitivities obtained in zebrafish are maintained in the rodent model. Our data also illustrate how KRAS mutations can provide proliferation advantages in relation to KRASWT and how chemotherapy can unbalance this advantage, selecting for a minor clone resistant to chemotherapy. Zebrafish xenografts provide remarkable resolution to measure Cetuximab sensitivity. Finally, we demonstrate the feasibility of using primary patient samples to generate zebrafish patient-derived xenografts (zPDX) and provide proof-of-concept experiments that compare response to chemotherapy and biological therapies between patients and zPDX. Altogether, our results suggest that zebrafish larvae xenografts constitute a promising fast assay for precision medicine, bridging the gap between genotype and phenotype in an in vivo setting.

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