scholarly journals MOLECULAR CHARACTERIZATION OF WHEAT GENOTYPES (TRITICUM AESTIVUM L.)

2017 ◽  
Vol 8 ◽  
pp. 29-36
Author(s):  
SHARAD PAWAR, MUNNESHKUMAR, V. M. JAMBHALE
Keyword(s):  
Dna Isolation ◽  
Pcr Amplification ◽  
Triticum Aestivum L ◽  
Dice Similarity Coefficient ◽  
Wheat Genotypes ◽  
Genomic Dna Isolation ◽  
Ssr Primers ◽  
Minimum Number ◽  
Maximum Similarity

Molecular diversity in thirty wheat genotypes was done. For this the genomic DNA isolation was carried out and which were then subjected to PCR amplification using twenty SSR primers. Out of these twenty SSR primers, eighteen yielded amplifications and showed polymorphism. Total 93 loci were generated by amplification with 18 polymorphic primers, all of which 93 loci were polymorphic i.e. 100%. Among the SSR primers, BARC-170, WMC-44, produced maximum number of 2 loci. The size of amplification products ranged from 102 bp to 805 bp. All SSR primers showed 100 % polymorphism and all primers had more than 0.50 PIC value except one primer. Maximum PIC value 0.17 was observed in WMC-468. The maximum number of bands were observed in NIAW-2721 (28 bands), whereas minimum number of bands were present in NIAW-301 and NIAW-2539 (19 bands). The dice similarity coefficient values ranged from 0.50 to 0.95. Maximum similarity value of 0.95 was noticed between NIAW-2891 and NIAW-2837, while minimum similarity value of 0.50 was observed among NIAW-2595, NIAW-2874, NIAW-2995 and NIAW-2725. The consensus tree software revealed two major clusters.

scholarly journals Molecular Typing of Staphylococcus Aureus Isolated from Various Environmental Sources

2020 ◽  
Vol 5 (6) ◽  
pp. 1243-1248
Author(s):  
Ingilala Satheesh Kumar ◽  
Nadakuditi Venkata Raju ◽  
Hanumohan R. Konatham
Keyword(s):  
Molecular Typing ◽  
Dna Isolation ◽  
Profile Analysis ◽  
Epidemiological Studies ◽  
Genomic Dna Isolation ◽  
Rapd Pcr ◽  
Pure Cultures ◽  
Host Origin ◽  
Different Sources

RAPD-PCR was employed for the characterization of S. aureus isolates from different sources such as soil, water, milk, meat and skin swab etc.,. Isolated pure cultures of S.aureus strains were subjected to genomic DNA isolation, purification, separation and quantification. Isolated DNA samples were distinguished by using 4 different random primers. Genome profile analysis obtained from the S.aureus demonstrated that it was possible to differentiate the S. aureus strains from different sources by RAPD technique. Results indicate possible relationships between host origin and genetic variation among S. aureus isolates from various sources. This RAPD method was thus useful for epidemiological studies of the S. aureus flora.

scholarly journals Comportement Phénologique et MorphoPhysiologique de Quelques Génotypes d’orge et de blé

2017 ◽  
Vol 13 (6) ◽  
pp. 287
Author(s):  
Zerafa Chafia ◽  
Ghenai Awatef ◽  
Benlaribi Mostefa
Keyword(s):  
Plant Genetic Resources ◽  
Data Bank ◽  
Triticum Aestivum L ◽  
Hordeum Vulgare L ◽  
Sowing Time ◽  
Wheat Genotypes ◽  
Triticum Durum Desf ◽  
Vegetable Material ◽  
Made In

The study which we propose on this agro-diversity requires precise and methodological characterization of some specimens of germplasm. Thus, a number of characteristics relating to the vegetative apparatus, the reproductive system, and the grain were quantified throughout the life cycle of the plant. This is to say that it takes place from the sowing time to the time of maturation of the caryopsis (Grain). The description of three durum wheat genotypes (Triticum durum Desf.), three common wheat genotypes (Triticum aestivum L.), and three of the barley genotypes (Hordeum vulgare L.) was carried out. This was in addition to phenology as well as the production characteristics (Agronomic characteristics) and adaptation characteristics. In particular, water deficit was adopted by the Vegetals Obtentions Protections Union (UPOV) from the year 1981. Also, it was expressed by Soltner in 1982 and 2005. Results obtained through the descriptive sheets and phenology show the existence of interesting intra and inter-specific variability that must be preserved, restored, and valorized. This is, however, performed on the vegetable material that are still available and whose genetic potential must be known precisely. Subsequently, we can suggest that the knowledge of cultivar phenology as well as the establishment of its descriptive sheet, as proposed by UPOV, is valuable as the prerequisite for any breeding program. Moreover, this constitutes a data-bank tow whose reference is made in the preservation and conservation of plant genetic resources.

scholarly journals Optimizing the pure genomic DNA isolation procedure for Plectranthus amboinicus DNA – A prerequisite for further genomic Studies

2017 ◽  
Vol 2 (4) ◽  
Author(s):  
S. Manikandan ◽  
S. Ansarali ◽  
G.M. Alagu Lakshmanan
Keyword(s):  
Dna Barcoding ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Pcr Amplification ◽  
Leaf Tissue ◽  
Isolation Procedure ◽  
Modified Method ◽  
Genomic Dna Isolation ◽  
Young Leaves ◽  
Plectranthus Amboinicus

The DNA isolation procedure for different plant groups have been studied and standardized.  The isolation of pure genomic DNA is the most essential component for any type of molecular studies.  The present work is aimed to identify suitable DNA markers for the amplification of P.amboinicus  DNA  becomes a great hurdle  for DNA barcoding studies carried out by rbcL and matK primers Used in the members of Lamiaceae. To solve this problem, The DNA was extracted by three methods from fresh young leaf tissue of P.amboinicus. After the evaluationthe outcome of these methods, one most suitable modified method was selected for isolating DNA from young leaves of P.amboinicus and selected for suitable DNA barcoding markers for PCR amplification. The quality and quantity of DNAs are a prerequisite for genetic studies for a variety of plants including P.amboinicus. The quantity and quality of the DNA extracted by this method wasused for suitable DNA barcoding markers selection.

scholarly journals Optimizing Genomic DNA Isolation and PCR Amplification For Pasak Bumi (Eurycoma longifolia)

2019 ◽  
Vol 1 (2) ◽  
pp. 30
Author(s):  
Arida Susilowati ◽  
Henti Hendalastuti Rachmat ◽  
Ahmad Baiquni Rangkuti ◽  
Deni Elfiati ◽  
Ami Ambarwati
Keyword(s):  
Genomic Dna ◽  
Dna Isolation ◽  
Pcr Amplification ◽  
Eurycoma Longifolia ◽  
Genomic Dna Isolation

.

scholarly journals Comparative Analysis of the Genomic DNA Isolation Methods on Inula sp. (Asteraceae)

2016 ◽  
Vol 8 (4) ◽  
pp. 451-455
Author(s):  
Emre SEVİNDİK ◽  
Fatih COŞKUN ◽  
Zehra Tuğba MURATHAN ◽  
Mehmet Yavuz PAKSOY ◽  
Veysel UZUN
Keyword(s):  
Genomic Dna ◽  
Dna Isolation ◽  
Low Cost ◽  
Pcr Amplification ◽  
Isoamyl Alcohol ◽  
Dna Amount ◽  
Genomic Dna Isolation ◽  
Asteraceae Family ◽  
Positive Results ◽  
Commercial Kit

Simple, fast, low-cost and high throughput protocols are required for DNA isolation of plant species. In this study, phenol chloroform isoamyl alcohol and commercial (Sigma) DNA isolation kit methods were applied on some Inula species that belong to Asteraceae family. Genomic DNA amounts, A260, A280, A260/A230 and purity degrees (A260/A280) that were obtained through both methods were measured through electrophoresis and spectrophotometer. Additionally, PCR amplification was realized by primer pairs specific to nrDNA ITS, cpDNA ndhF (972F-1603R) and trnL-F regions. Results showed that maximum genomic DNA in nanograms obtained by phenol chloroform isoamyl alcohol method. The study also revealed that I. macrocephala had the maximum DNA and I. heterolepis had the minimum DNA amount. A260/A280 purity degrees showed that the highest and lowest purity in gDNAs obtained through phenol-choloform isoamyl alcohol method were in I.aucheriana and I. salicina, respectively. The highest and lowest purity degrees of gDNAs obtained through commercial kit was observed in I. fragilis and I. macrocephala samples, respectively. PCR amplification results showed that while band profiles of each three regions (ITS, trnL-F and ndhF) did not yield positive results in PCR amplifications using phenol-choloform isoamyl alcohol method; PCR band profiles obtained through commercial kit yielded positive results. As a result, it is fair to say that the relation of genomic DNA with PCR was found to be more efficient although the maximum amount of genomic DNA was obtained through phenol chloroform isoamyl alcohol method. 

scholarly journals Development of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Species

2016 ◽  
Vol 4 (8) ◽  
pp. 700 ◽  
Author(s):  
Muhammad Amjad Nawaz ◽  
Faheem Shehzad Baloch ◽  
Hafiz Mamoon Rehman ◽  
Bao Le ◽  
Fahima Akther ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Dna Extraction ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Plant Molecular Biology ◽  
Pcr Amplification ◽  
Cost Effective ◽  
Extraction Methods ◽  
Glycine Species ◽  
Genomic Dna Isolation

Extraction of deoxyribose nucleic acid (DNA) from plants is preliminary step in molecular biology. Fast and cost effective genomic DNA isolation from Glycine species for downstream application is a major bottleneck. Here we report a high throughput and trouble free method for genomic DNA extraction from leaf and seeds of Glycine species with high quality and quantity. Protocol reports the optimization by employing different concentrations of CTAB and PVP in extraction buffer. Efficiency of optimized protocol was compared with frequently used DNA extraction methods. Wide adoptability and utility of this protocol was confirmed by DNA extraction from leaves as well as seeds of G. max, G. soja, G. tomentella and G. latifolia. Extracted DNA was successfully subjected to PCR amplification of five microsatellite markers and four putative glycosyltransferase genes. DNA extraction protocol is reproducible, trouble free, rapid and can be adopted for plant molecular biology applications.

Characterization of a cDNA for Rhesus Monkey Protein C Inhibitor – Evidence for N-Terminal Involvement in Heparin Stimulation

1995 ◽  
Vol 74 (04) ◽  
pp. 1079-1087 ◽  
Author(s):  
Klaus-P Radtke ◽  
José A Fernández ◽  
Bruno O Villoutreix ◽  
Judith S Greengard ◽  
John H Griffin
Keyword(s):  
Rhesus Monkey ◽  
Protein C ◽  
Activated Protein C ◽  
Pcr Amplification ◽  
Amino Acid Sequences ◽  
Heparin Binding ◽  
Reactive Center ◽  
Protein C Inhibitor ◽  
Polymerase Chain

SummarycDNAs for protein C inhibitor (PCI) were cloned from human and rhesus monkey 1 liver RNAs by reverse transcription and polymerase chain reaction (PCR) amplification. Sequencing showed that rhesus monkey and human PCI cDNAs were 93% identical. Predicted amino acid sequences differed at 26 of 387 residues. Pour of these differences (T352M, N359S, R362K, L3631) were in the reactive center loop that is important for inhibitory specificity, and two were in the N-terminal helix (M8T, E13K) that is implicated in glycosaminoglycan binding. PCI in human or rhesus monkey plasma showed comparable inhibitory activity towards human activated protein C in the presence of 10 U/ml heparin. However, maximal acceleration of the inhibition of activated protein C required 5-fold lower heparin concentration for rhesus monkey than for human plasma, consistent with the interpretation that the additional positive charge (E13K) in a putative-heparin binding region increased the affinity for heparin.

Genomic Dna Isolation From Plumbago L. Species Growing In India

2012 ◽  
Vol 2 (7) ◽  
pp. 45-46
Author(s):  
Varsha N Nathar ◽  
◽  
Prashant J Gadge
Keyword(s):  
Genomic Dna ◽  
Dna Isolation ◽  
Genomic Dna Isolation

scholarly journals Isolation and characterization of two virulent Aeromonads associated with haemorrhagic septicaemia and tail-rot disease in farmed climbing perch Anabas testudineus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Abhishek Mazumder ◽  
Hrishikesh Choudhury ◽  
Abhinit Dey ◽  
Dandadhar Sarma
Keyword(s):  
Natural Infection ◽  
Winter Season ◽  
Pcr Amplification ◽  
Body Parts ◽  
Anabas Testudineus ◽  
Climbing Perch ◽  
Isolation And Characterization ◽  
Biochemical Characterisation ◽  
Rot Disease

AbstractDiseased Anabas testudineus exhibiting signs of tail-rot and ulcerations on body were collected from a fish farm in Assam, India during the winter season (November 2018 to January 2019). Swabs from the infected body parts were streaked on sterilized nutrient agar. Two dominant bacterial colonies were obtained, which were then isolated and labelled as AM-31 and AM-05. Standard biochemical characterisation and 16S rRNA and rpoB gene sequencing identified AM-31 isolate as Aeromonas hydrophila and AM-05 as Aeromonas jandaei. Symptoms similar to that of natural infection were observed on re-infecting both bacteria to disease-free A. testudineus, which confirmed their virulence. LC50 was determined at 1.3 × 104 (A. hydrophila) and 2.5 × 104 (A. jandaei) CFU per fish in intraperitoneal injection. Further, PCR amplification of specific genes responsible for virulence (aerolysin and enterotoxin) confirmed pathogenicity of both bacteria. Histopathology of kidney and liver in the experimentally-infected fishes revealed haemorrhage, tubular degeneration and vacuolation. Antibiotic profiles were also assessed for both bacteria. To the best of our knowledge, the present work is a first report on the mortality of farmed climbing perch naturally-infected by A. hydrophila as well as A. jandaei, with no records of pathogenicity of the latter in this fish.

Sign in / Sign up

Export Citation Format

Share Document