scholarly journals ВМІСТ ФОТОСИНТЕТИЧНИХ ПІГМЕНТІВ У РОСЛИНАХ РОДУ CARLINA L. У ПРИРОДІ ТА КУЛЬТУРІ IN VITRO

2020 ◽  
Vol 78 (4) ◽  
pp. 16-23
Author(s):  
N. B. Kravets ◽  
L. R. Hrytsak ◽  
M. Z. Prokopyak ◽  
O. Yu. Mayorova ◽  
N. M. Drobyk
Keyword(s):  
Acid Solution ◽  
Growth Regulators ◽  
Root Formation ◽  
Cultivated Plants ◽  
Presowing Treatment ◽  
Murashige Skoog ◽  
Half Strength ◽  
Semi Solid ◽  
Positive Effect

im. The aim of the study was to choose conditions for rooting improvement of in vitro cultivated plants of some species of Carlina L. genus. Methods. For receiving and rooting of aseptic sprouts, seeds of Carlina acaulis L., Carlina cirsioides Klok and Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl were subjected to presowing treatment with gibberellic acid solution (GA3) or indolebyturic acid solution (IBA). Sterilized seeds were planted in sterile Petri dishes on semi-solid Murashige, Skoog nutrient medium with half-strength concentrations of macro- and microsalts without growth regulators. Results. It was found that with the seed soaking of C. acaulis, C. cirsioides and C. onopordifolia in GA3 solution the percentage of root formation amounted to 33.3 %, 33.3 % and 22.2 % respectively. Presowing treatment of carlina seeds in IBA solution with concentration of 1000 mg for 2–4 hours before sterilization gave a positive effect: the percentage of root formation for C. acaulis, C. cirsioides and C. onorordifolia was 2.4–4.5 times higher compared to the treatment with GA3 solution. Conclusions. To form the root system of carlina plants it is effective to soak the seeds in the solution of IBA. Thus we were able to increase the percentage of rooting of C. sirsioides and C. onorordifolia plants to 100 %, C. acaulis plants – up to 80 % and avoid sprouts’ injury and changes in the concentrations of the IBA, which may occur during sterilization at high temperatures by using non-sterile solution of growth regulators.

scholarly journals Peculiarities of in vitro rooting of some species of Carlina L. genus

1970 ◽  
pp. 215-220
Author(s):  
N. B. Kravets ◽  
M. Z. Mosula ◽  
N. M. Drobyk ◽  
N. V. Tulaidan ◽  
M. B. Cheterbok
Keyword(s):  
Acid Solution ◽  
Growth Regulators ◽  
Root Formation ◽  
Cultivated Plants ◽  
Treat Ment ◽  
Presowing Treatment ◽  
Murashige Skoog ◽  
Half Strength ◽  
Semi Solid

Aim. The aim of the study was to choose conditions for rooting improvement of in vitro cultivated plants of some species of Carlina L. genus. Methods. For receiving and rooting of aseptic sprouts, seeds of Carlina acaulis L., Carlina cirsioides Klok and Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl were subjected to presowing treatment with gibberellic acid solution (GA3) or indolebyturic acid solution (IBA). Sterilized seeds were planted in sterile Petri dishes on semi-solid Murashige, Skoog nutrient medium with half-strength concentrations of macro- and microsalts without growth regulators. Results. It was found that with the seed soaking of C. acaulis, C. cirsioides and C. onopordifolia in GA3 solution the percentage of root formation amounted to 33.3 %, 33.3 % and 22.2 % respectively. Presowing treat-ment of carlina seeds in IBA solution with concentration of 1000 mg for 2–4 hours before sterilization gave a positive effect: the percentage of root formation for C. acaulis, C. cirsioides and C. onorordifolia was 2.4–4.5 times higher compared to the treatment with GA3 solution. Conclusions. To form the root system of carlina plants it is effective to soak the seeds in the solution of IBA. Thus we were able to increase the percentage of rooting of C. sirsioides and C. onorordifolia plants to 100 %, C. acaulis plants – up to 80 % and avoid sprouts’ injury and changes in the concentra-tions of the IBA, which may occur during sterilization at high temperatures by using non-sterile solution of growth regulators. Keywords: Carlina àcaulis L., Carlina cirsioides Klok, Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl, in vitro, sprouts rooting.

scholarly journals Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne

2022 ◽  
Author(s):  
Monika Poniewozik ◽  
Marzena Parzymies ◽  
Paweł Szot
Keyword(s):  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Activated Charcoal ◽  
Root Formation ◽  
Multiplication Rate ◽  
Ms Medium ◽  
In Vitro Morphogenesis ◽  
Half Strength ◽  
Positive Effect

Phenolic compounds limit micropropagation of many orchids in vitro. The aim of the study was to estimate the effect of activated charcoal (AC);1, 2 or 4 g/L) or ascorbic acid (AA; 10, 20 or 30 mg/L) added to the half strength MS medium on the growth and o-dihydroxyphenols content in Paphiopedilum insigne in vitro. A positive effect of AC on the shoot and root formation has been found. The highest multiplication rate (5.6 shoots/explant) and rooting frequency were obtained on medium containing 2 g/L of AC. However, AC reduced the leaf number as compared to the control. The lowest content of o-dihydroxyphenols was marked in Paphiopedilum insigne leaves when the shoots were grown on medium with 10 mg/L AA, followed by AC at 1 or 2 g/L.

scholarly journals Haploid plantlet regeneration through anther culture in oilseed Brassica species

1970 ◽  
Vol 34 (4) ◽  
pp. 693-703 ◽  
Author(s):  
MA Alam ◽  
MA Haque ◽  
MR Hossain ◽  
SC Sarker ◽  
R Afroz
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Growth Regulators ◽  
Callus Induction ◽  
Brassica Species ◽  
Plantlet Regeneration ◽  
Root Induction ◽  
Ms Medium ◽  
Half Strength

Anther of five varieties of Brassica species, namely BARI Shariaha-7, Tori-7, Agrani, Daulat and Safal were cultured in vitro to observe their regeneration potentiality. Different concentrations and combinations of growth regulators were supplemented in MS medium. The range of callus induction was 12.50-87.50 %. Maximum callus induction (75.00%) was observed on MS +4 mg/L 2, 4-D + 1.0 mg/L BAP. Among the genotypes, BARI Sharisha-7 showed the highest percentage of callus induction (60.42%). Among the treatments, highest percentage of shoot regeneration (75.00%) was observed on MS + 4 mg/L BAP + 1.0 mg/L NAA. BARI Sharisha-7 also showed the highest rate of plant regeneration (66.67%). Root induction was highest (75%) on half strength MS medium supplemented with 1.0 mg/L IBA and 0.5 mg/L NAA. The plantlets with sufficient roots thus obtained were transferred successfully to plastic pots and subsequently to the field. BARI Sharisha-7 and Tori-7 survived easily in the pots as well as in the field but Safal was very poor in survivability both in the pots and in the field. Key Words: Brassica; haploid; anther culture; in vitro regeneration.DOI: 10.3329/bjar.v34i4.5844Bangladesh J. Agril. Res. 34(4) : 693-703, December 2009 

scholarly journals Improved micropropagation and foliar micromorphological studies in Turnera ulmifolia L. – An important medicinal plant

2018 ◽  
Vol 30 (2) ◽  
pp. 283-294 ◽  
Author(s):  
Mani Manokari ◽  
Mahipal S. Shekhawat
Keyword(s):  
Shoot Proliferation ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Half Strength ◽  
Turnera Ulmifolia ◽  
Semi Solid ◽  
Number Of Shoots

Abstract The present study reports an efficient in vitro propagation system for Turnera ulmifolia using nodal segments as explants. Turnera ulmifolia (Passifloraceae) is an important garden plant with multipotent medicinal values. Effective shoot proliferation was achieved on agar gelled MS medium (Murashige and Skoog, 1962). The maximum number of shoots (8.3 ± 0.57) per initial explant was obtained on MS medium supplemented with 8.88 mM of 6-benzylaminopurine (BAP) and 0.54 mM of α-naphthalene acetic acid (NAA). The highest number of shoots (59.5 ± 2.10) proliferated on semi-solid MS medium (with agar) augmented with 2.22 mM of BAP and 2.32 mM of kinetin (Kin) along with 0.54 mM of NAA. Longer (4-5 cm) and healthy shoots were rooted (12.0 ± 0.10 roots per shoot) on half-strength MS medium fortified with 9.84 mM of indole-3 butyric acid (IBA). The in vitro regenerated plantlets were hardened in the greenhouse and transferred to the field. Significant developmental changes were observed in the foliar micromorphology of in vitro raised plantlets when these were transferred to the field. The stomatal index was gradually reduced (26.72 to 21.25) in the leaves from in vitro to field environments. But, vein-islets and veinlet terminations (13.4 and 7.6) were increased (39.7 and 18.4) respectively from in vitro to in vivo grown plants. Simple, unicellular, less frequent and underdeveloped trichomes were observed with the leaves of in vitro plants but fully developed trichomes recorded in the field transferred plants. The study could help in understanding the response and adaptation of tissue culture raised plantlets towards changed environmental conditions.

scholarly journals The effects of growth regulators in proliferation of Sorbus redliana 'Burokvölgy'

2006 ◽  
Vol 12 (1) ◽  
Author(s):  
M. Ördögh ◽  
E. Jámbor-Benczúr ◽  
A. Tilly Mándy ◽  
L. Lelik
Keyword(s):  
Growth Regulators ◽  
Optimal Growth ◽  
Optimum Concentration ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Dark Light ◽  
Chlorophyll Contents ◽  
Half Strength ◽  
Number Of Shoots

The Hungarian cultivar Sorbus redliana 'Burokvölgy' was proliferated on Murashige and Skoog (MS, 1962) medium with half-strength macroelements and 100 mg/1 meso-inositol, 20 g/1 sucrose, 11 g/1 agar-agar. Different combinations of kinetin (KIN), metatopolin (mT), benzyladenine (BA), benzyladenine-ribosid (BAR) and indolebutiric acid (IBA) were tested, and pH was adjusted to 5.6 every case using KOH. The cultures were incubated at 20-24 °C in 8/16 hours dark/light photoperiod for 50-52 days. The main aim of our research was to find the optimal growth regulator and its optimum concentration. Purthermore, to determine the chlorophyll contents of the in vitro propagated plants' leaves. During the proliferation, the highest number of shoots were observed in the case of using BA + IBA, and on the medium containing 0.75 mg/I BA + 0.05 mg/1 IBA 8.93 shoots were found. The addition of KIN + IBA decreased the number of shoots and increased the sizes of leaves — the widest (11.2 mm) and longest (17.8 mm) leaves were obtained on the medium containing 1.00 mg/I KIN + 0.05 mg/1 IBA. The longest shoots (36.46 mm) were found in the case of applying 0.75 mg/1 BAR + 0.05 mg/I IBA. The BA + KIN + IBA combination resulted the shortest shoots. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. The highest chlorophyll content (1.569 mg/g total chlorophyll, 1.132 mg/g chlorophyll-a, 0.437 mg/g chlorophyll-b) was obtained in the presence of 1.0 mg/I KIN + 0.05 mg/1 IBA.

scholarly journals Pertumbuhan Biji Buah Naga (Hylocereus Polyrhizus) dengan Pemberian NAA dan Ekstrak Biji Jagung (Zea Mays) secara In Vitro

2021 ◽  
Vol 11 (1) ◽  
pp. 47
Author(s):  
Rizka Widasari ◽  
Mukarlina Mukarlina ◽  
Zulfa Zakiah
Keyword(s):  
Seed Germination ◽  
Growth Regulators ◽  
Seed Extract ◽  
Cultivated Plants ◽  
Corn Seed ◽  
Germination Time ◽  
M 10 ◽  
Dragon Fruit ◽  
Hylocereus Polyrhizus

(Article History: Received 25 November 2020; Revised 5 January 2021; Accepted 10 February 2021) ABSTRAKBuah naga (Hylocereus polyrhizus) merupakan salah satu tanaman yang dibudidayakan di Indonesia. Perbanyakan bibit secara in vitro dengan teknologi kultur jaringan merupakan cara alternatif yang tepat untuk menyediakan bibit dalam waktu singkat dengan jumlah banyak. Penelitian ini bertujuan untuk menguji pengaruh dan konsentrasi terbaik penambahan ekstrak biji jagung dan NAA pada media tumbuh terhadap perkecambahan biji buah naga merah secara in vitro. Penelitian dilaksanakan di Laboratorium Kultur Jaringan, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Penelitian menggunakan Rancangan Acak Lengkap dengan 2 faktor perlakuan. Faktor pertama yaitu NAA dengan konsentrasi 0 M, 10-8M, 10-7M, dan 5x10-7M. Faktor kedua yaitu ekstrak biji jagung dengan konsentrasi 0%, 5%, 7,5%, dan 10%. Hasil penelitian menunjukkan pemberian ekstrak biji jagung tunggal, NAA tunggal dan kombinasi ekstrak biji jagung dan NAA memberikan pengaruh nyata terhadap tinggi planlet, namun tidak berpengaruh nyata terhadap persentase perkecambahan. Pemberian ekstrak biji jagung tunggal memberikan pengaruh nyata terhadap waktu berkecambah, sedangkan pemberian ekstrak biji jagung tunggal dan NAA tunggal berpengaruh nyata terhadap jumlah akar. Perlakuan  5.10-7M NAA + 7,5% ekstrak biji jagung menghasilkan pertumbuhan tinggi plantlet terbaik. Konsentrasi 7,5% ekstrak biji jagung merupakan konsentrasi terbaik waktu berkecambahan biji buah naga dan konsentrasi NAA tunggal 10-8M memberikan jumlah akar terbanyak.Kata Kunci: Pertumbuhan in vitro, Biji Buah Naga, Ekstrak Biji Jagung, NAA.                                     ABSTRAKDragon fruit (Hylocereus polyrhizus) is one of the cultivated plants in Indonesia. In vitro propagation of seeds with the addition of synthetic growth regulators or from growth regulators from organic matter in the media, is an appropriate alternative way to provide large quantities of seeds in a short time. This study aims to determine the effect and the best effect of corn seed extract and NAA on growing media on in vitro red dragon fruit seed germination. The research was conducted at the Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Tanjungpura Pontianak. The study used a completely randomized design with 2 treatment factors. The first factor is NAA with a concentration of 0 M, 10-8M, 10-7M, and 5x10-7M. The second factor is corn seed extract with a concentration of 0%, 5%, 7.5%, and 10%. The results showed that the administration of single corn seed extract, single NAA and a combination of corn seed extract and NAA had a significant effect on plantlet height, but did not significantly affect the germination percentage. The administration of single corn seed extract had a significant effect on germination time, while the administration of single corn seed extract and single NAA had a significant effect on the number of roots. The 5.10-7M NAA + 7.5% corn seed extract treatment resulted in the best plantlet height growth. The concentration of 7.5% corn seed extract was the best concentration for dragon fruit seed germination time and a single NAA concentration of 10-8M gave the highest number of roots.Keywords: In Vitro Growth, Dragon Fruit Seeds, Corn Seed Extract, NAA.

scholarly journals In Vitro Development of the Rare and Endangered Moss Molendoa hornschuchiana (Hook.) Lindb. ex Limpr. (Pottiaceae, Bryophyta)

HortScience ◽  
2012 ◽  
Vol 47 (1) ◽  
pp. 84-87 ◽  
Author(s):  
Milorad Vujičić ◽  
Aneta Sabovljević ◽  
Jasmina Šinžar-Sekulić ◽  
Marijana Skorić ◽  
Marko Sabovljević
Keyword(s):  
Butyric Acid ◽  
Growth Regulators ◽  
Ex Situ ◽  
High Mountain ◽  
Natural Habitats ◽  
Rare And Endangered ◽  
Half Strength ◽  
Vitro Development ◽  
Maximum Development

The high mountain pottioid moss Molendoa hornschuchiana (Hook) Lindb. ex Limpr. is a very rare and critically endangered bryophyte species in Europe in need for ex situ conservation. A 25-year-old herbarium sample was used to revive and propagate this species for further reintroduction and introduction to potential natural habitats. The reviving of “dead” herbarium specimen was achieved by disposing of axenical organisms as well as adjusting condition for developing secondary protonema, bud inductions, and optimization of gametophyte propagation in vitro condition.The influence of exogenously added growth regulators on the morphogenesis of this species was studied. The plants were cultured in the two basic types of media, viz., BCD and half-strength Murashige and Skoog (MS) supplemented with different concentrations (0.01–0.3 μM) of indole-3-butyric acid (IBA) and N6-benzyladenine (BA) under a 16-h photoperiod. The influence of growth regulators on gametophores multiplication in vitro as well as on protonemal diameter was recorded. Well-developed gametophores were obtained on BCD medium, whereas on half-strength MS medium, secondary protonema was produced, both on hormone-free and supplemented substrate exclusively. Based on multiplication index in vitro, maximum development of gametophores was realized on BCD medium supplemented with 0.3 μM IBA and 0.1 μM BA. However, the widest diameter of secondary protonema was obtained on BCD medium enriched with low concentration of both BA (0.01 and 0.03 μM) and constant concentration of IBA (0.03 μM). Chemical names used: indole-3-butyric acid (IBA), N6-benzyladenine (BA), Murashige and Skoog medium (MS).

scholarly journals In vitro: Influence of Various Concentrations of Plant Growth Regulators (BAP & NAA) and Sucrose on Regeneration of Chenopodium quinoa Willd. Plant

2020 ◽  
pp. 34-43
Author(s):  
Fayza R. Al Gethami ◽  
Hameda El Sayed Ahmed El Sayed
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Chenopodium Quinoa ◽  
Naphthalene Acetic Acid ◽  
Cotyledonary Nodes ◽  
Half Strength ◽  
The Media

In vitro: regeneration of Chenopodium quinoa Willd. was achieved from cotyledonary nodes explants. In this study, used 6-Benzylaminopurine (BAP) and α-Naphthalene Acetic Acid (NAA) of plant growth regulators with different concentrations individually as well as in combination and used different concentrations of sugar (sucrose) with different concentrations. For was rooting, used half strength (½MS), full-strength MS and ½ MS supplemented with 0.2 mg/l of NAA. The results mentioned, explant responding (%) to multiplication was about 73% for all BAP treatments compared with control and average numbers of shoot increased with increased BAP concentration except 5 mg/l of BAP. The highest explant responding (%) was in media supplemented BAP without NAA compared other treatments noted that the media with combination of BAP and NAA gives formation of callus in bases of the plantlets. Also, the result inducted the combinations between (BAP–NAA) was highly significantly (P≤ 0.001) and less effective on number of shoots where the highest number of shoot was 3.40 in media with 3 mg/l BAP compared other treatments. The highest of explant responding 93.33% was in media supplement with 10 g/l sucrose and (10 g/l sucrose + 3 mg/l BAP), but sucrose level for good greening and developed shoots (4 shoots) was in medium supplement with 10 g/l sucrose. The shoots rooted well on half-strength MS medium with 60% percentage of root. The rooted shoots were acclimatized and transferred to green house to follow their development.

scholarly journals Influence of presowing treatment of seeds by growth regulators and fungicides on field similarity of Zinnia elegans

2020 ◽  
pp. 44-49
Author(s):  
Nikolay Vasilievich Smolin ◽  
Yulia Nikolaevna Nedaiborshch ◽  
Natalya Vasilievna Potapova ◽  
Andrey Sergeevich Saveliev ◽  
Victor Vladimirovich Volgin ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Seed Treatment ◽  
Germination Rate ◽  
Stress Factors ◽  
Juvenile Period ◽  
Presowing Treatment ◽  
Stress Components ◽  
Rate Of Emergence ◽  
The One ◽  
Positive Effect

The article discusses the effect of presowing seed treatment by growth regulators and fungicides on the field germination of seeds of two grades of zinnia elegant – Dream and Orange King. Varieties differed in seed germination time. On average, over three years, Zinnia Dream seeds germinated 0.5–1.0 days earlier than Orange King seeds. The treatment of zinnia seeds with an epin-extra growth regulator contributed to the emergence of earlier seedlings. When the seeds were soaked with a solution of this preparation, the plants went through the sprouting phase faster – the period when they are most sensitive to abiotic stresses. Zircon had a weaker effect on the germination rate of zinnia seeds than epin-extra, however, the presence of a prolonged action of this drug in the activation of non-specific resistance of plants to stress factors can also be noted. The one-component disinfectant benorad turned out to be the most passive and did not significantly affect the germination of zinnia. There were no visible signs of manifestation of pathogens in the juvenile period of plants, and its active substance (benomyl) could hardly affect the rate of emergence of seedlings. In contrast to benorad, the two-component protectant vial TrasT had a weak positive effect on the rate of emergence of seedlings, since it included specially introduced anti-stress components.

scholarly journals In Vitro Regeneration of Miscanthus x giganteus through Indirect Organogenesis: Effect of Explant Type and Growth Regulators

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2799
Author(s):  
Aušra Blinstrubienė ◽  
Inga Jančauskienė ◽  
Natalija Burbulis
Keyword(s):  
Growth Regulators ◽  
Root Formation ◽  
In Vitro Regeneration ◽  
Agricultural Crop ◽  
Explant Type ◽  
Breeding Methods ◽  
Miscanthus X Giganteus ◽  
Indirect Organogenesis ◽  
Morphogenic Callus

Miscanthus x giganteus is a spontaneous sterile hybrid therefore the creation of useful genetic diversity by conventional breeding methods is restricted. Plant regeneration through indirect organogenesis may be a useful approach to create genetic variability of this important agricultural crop. The present study aimed to evaluate the effect of the explant type and growth regulators on indirect organogenesis of Miscanthus x giganteus and to determine the ploidy level of plant regenerants by flow cytometry. On average, the highest percentage of morphogenic callus tested explants formed in the medium supplemented with 2.5 mg L–1 IBA + 0.1 mg L–1 BAP + 4.0 mg L–1 l-proline. The most intensive secondary differentiation of callus cells was observed in the medium supplemented with 4.0 mg L–1 ZEA + 1.0 mg L–1 NAA. The highest root formation frequency with the highest number of roots was determined in the MS nutrient medium supplemented with 0.4 mg L–1 IBA, where more than 95% of plant regenerants survived and were growing normally.

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