BIG COFFEE VL.: SEED DESICCATION TOLERANCE, SIEVE CLASSIFICATION, AND PHYSIOLOGICAL QUALITY

Norway maple and sycamore produce desiccation-tolerant (orthodox) and desiccation-sensitive (recalcitrant) seeds, respectively. Drying affects reduction and oxidation (redox) status in seeds. Oxidation of methionine to methionine sulfoxide (MetO) and reduction via methionine sulfoxide reductases (Msrs) have never been investigated in relation to seed desiccation tolerance. MetO levels and the abundance of Msrs were investigated in relation to levels of reactive oxygen species (ROS) such as hydrogen peroxide, superoxide anion radical and hydroxyl radical (•OH), and the levels of ascorbate and glutathione redox couples in gradually dried seeds. Peptide-bound MetO levels were positively correlated with ROS concentrations in the orthodox seeds. In particular, •OH affected MetO levels as well as the abundance of MsrB2 solely in the embryonic axes of Norway maple seeds. In this species, MsrB2 was present in oxidized and reduced forms, and the latter was favored by reduced glutathione and ascorbic acid. In contrast, sycamore seeds accumulated higher ROS levels. Additionally, MsrB2 was oxidized in sycamore throughout dehydration. In this context, the three elements •OH level, MetO content and MsrB2 abundance, linked together uniquely to Norway maple seeds, might be considered important players of the redox network associated with desiccation tolerance.

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Seed desiccation tolerance and dispersal in tropical dry forests in Colombia: Implications for ecological restoration

Forest Ecology and Management ◽

10.1016/j.foreco.2017.08.042 ◽

2017 ◽

Vol 404 ◽

pp. 289-293 ◽

Cited By ~ 5

Author(s):

Claudia Galindo-Rodriguez ◽

Lilia L. Roa-Fuentes

Keyword(s):

Ecological Restoration ◽

Desiccation Tolerance ◽

Tropical Dry Forests ◽

Dry Forests ◽

Seed Desiccation

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Pollen and seed desiccation tolerance in relation to degree of developmental arrest, dispersal, and survival

Journal of Experimental Botany ◽

10.1093/jxb/err154 ◽

2011 ◽

Vol 62 (15) ◽

pp. 5267-5281 ◽

Cited By ~ 77

Author(s):

G. G. Franchi ◽

B. Piotto ◽

M. Nepi ◽

C. C. Baskin ◽

J. M. Baskin ◽

...

Keyword(s):

Desiccation Tolerance ◽

Developmental Arrest ◽

Seed Desiccation

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Desiccation tolerance in relation to soluble sugar contents in seeds of ten coffee (CoffeaL.) species

Seed Science Research ◽

10.1017/s0960258500000428 ◽

2000 ◽

Vol 10 (3) ◽

pp. 393-396 ◽

Cited By ~ 11

Author(s):

Nathalie Chabrillange ◽

Stéphane Dussert ◽

Florent Engelmann ◽

Sylvie Doulbeau ◽

Serge Hamon

Keyword(s):

Desiccation Tolerance ◽

Coffea Arabica ◽

High Performance ◽

Sugar Content ◽

Soluble Sugar ◽

Soluble Sugars ◽

Dry Weight ◽

Seed Desiccation ◽

Desiccation Sensitivity ◽

Coffee Species

AbstractLarge differences in seed desiccation sensitivity have been observed previously among ten coffee species (Coffea arabica, C. brevipes, C. canephora, C. eugenioides, C. humilis, C. liberica, C. pocsii, C. pseudo-zanguebariae, C. sessiliflora and C.stenophylla). Of these species,C. libericaandC. humiliswere the most sensitive to desiccation andC. pseudozanguebariaethe most tolerant. A study was carried out using the same seed lots to investigate if these differences in desiccation tolerance could be correlated with differences in soluble sugar content. Soluble sugars were extracted from dry seeds and analysed using high performance liquid chromatography. The seed monosaccharide (glucose and fructose) content was very low (1.5 to 2 mg g-1dry weight [dw]) in all species studied. The sucrose content ranged from 33 mg g-1dw inC. libericaseeds to 89 mg g-1dw in seeds ofC. pocsii. Raffinose was detected in the seeds of only five species (C.arabica, C.brevipes, C.humilis, C.sessiliflora, C.stenophylla), among which only three species (C.arabica, C.sessilifloraandC.brevipes) also contained stachyose. Both raffinose and stachyose were present in very low quantities (0.3–1.4 mg g-1dw and 0.1–0.7 mg g-1dw, respectively). Verbascose was never detected. No significant relationship was found between seed desiccation sensitivity and: (i) the sugar content; (ii) the presence/absence of oligosaccharides; and (iii) the oligosaccharide:sucrose ratio.

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What Do We Know About the Genetic Basis of Seed Desiccation Tolerance and Longevity?

International Journal of Molecular Sciences ◽

10.3390/ijms21103612 ◽

2020 ◽

Vol 21 (10) ◽

pp. 3612

Author(s):

Hanna Kijak ◽

Ewelina Ratajczak

Keyword(s):

Low Temperature ◽

Desiccation Tolerance ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Seed Storage ◽

Water Losses ◽

Economic Interests ◽

Seed Desiccation ◽

Orthodox Seeds ◽

The Right

Long-term seed storage is important for protecting both economic interests and biodiversity. The extraordinary properties of seeds allow us to store them in the right conditions for years. However, not all types of seeds are resilient, and some do not tolerate extreme desiccation or low temperature. Seeds can be divided into three categories: (1) orthodox seeds, which tolerate water losses of up to 7% of their water content and can be stored at low temperature; (2) recalcitrant seeds, which require a humidity of 27%; and (3) intermediate seeds, which lose their viability relatively quickly compared to orthodox seeds. In this article, we discuss the genetic bases for desiccation tolerance and longevity in seeds and the differences in gene expression profiles between the mentioned types of seeds.

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Ecological correlates of seed desiccation tolerance in tropical African dryland trees

American Journal of Botany ◽

10.3732/ajb.91.6.863 ◽

2004 ◽

Vol 91 (6) ◽

pp. 863-870 ◽

Cited By ~ 81

Author(s):

Hugh W. Pritchard ◽

Matthew I. Daws ◽

Benjamin J. Fletcher ◽

Christiane S. Gaméné ◽

Heriel P. Msanga ◽

...

Keyword(s):

Desiccation Tolerance ◽

Seed Desiccation ◽

Ecological Correlates

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A Combination of Histological, Physiological, and Proteomic Approaches Shed Light on Seed Desiccation Tolerance of the Basal Angiosperm Amborella trichopoda

Proteomes ◽

10.3390/proteomes5030019 ◽

2017 ◽

Vol 5 (4) ◽

pp. 19 ◽

Cited By ~ 4

Author(s):

Matthieu Villegente ◽

Philippe Marmey ◽

Claudette Job ◽

Marc Galland ◽

Gwendal Cueff ◽

...

Keyword(s):

Desiccation Tolerance ◽

Basal Angiosperm ◽

Seed Desiccation ◽

Shed Light

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Physiological and biochemical changes during desiccation tolerance loss in millet (Pennisetum glaucum L.) seeds

Acta Scientiarum Agronomy ◽

10.4025/actasciagron.v41i1.36522 ◽

2019 ◽

Vol 41 (1) ◽

pp. 36522

Author(s):

Joana Souza Fernandes ◽

Renato Mendes Guimarães ◽

Jose Marcio Rocha Faria ◽

Diego De Sousa Pereira ◽

Stefania Vilas Boas Coelho ◽

...

Keyword(s):

Desiccation Tolerance ◽

Pennisetum Glaucum ◽

Seed Vigor ◽

Biochemical Changes ◽

Heat Resistant ◽

Randomized Design ◽

Enzymatic Systems ◽

Physiological Quality ◽

Catalase Peroxidase ◽

Physiological And Biochemical

The aim of this study was to evaluate the physiological and biochemical changes related to the loss of desiccation tolerance in millet seeds. The points studied were determined according to the seed imbibition curve of the hybrid ADRF6010: control (0 h), 3 h, 1 and 3 mm of radicle. The seeds were dried on silica gel for 72 h at 20 °C, followed by pre-humidification at 25 °C for 24 h. The physiological quality was evaluated by the electrical conductivity and germination test, and the seed vigor through the first count and germination speed index. The experiment was performed in a completely randomized design and means were compared by the Scott-knott test at 5% probability. The enzymatic systems evaluated were: superoxide dismutase, catalase, peroxidase, and α-amylase, and the expression of heat-resistant proteins. The enzymatic activity was quantified through the software ImageJ®. Millet seeds lost desiccation tolerance when the radicle reaches 1 mm of length. According to the enzymatic standards, the enzymes peroxidase and α-amylase, as well as the activity of heat-resistant proteins are related to the loss of desiccation tolerance in millet seeds.

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Evolutionary ecophysiology of seed desiccation sensitivity

Functional Plant Biology ◽

10.1071/fp18022 ◽

2018 ◽

Vol 45 (11) ◽

pp. 1083 ◽

Cited By ~ 9

Author(s):

Alexandre Marques ◽

Gonda Buijs ◽

Wilco Ligterink ◽

Henk Hilhorst

Keyword(s):

Desiccation Tolerance ◽

Molecular Mechanisms ◽

Genetic Alterations ◽

Tropical Rainforests ◽

Model Species ◽

Seed Desiccation ◽

Desiccation Sensitivity ◽

Selection For ◽

Shed Light

Desiccation sensitive (DS) seeds do not survive dry storage due to their lack of desiccation tolerance. Almost half of the plant species in tropical rainforests produce DS seeds and therefore the desiccation sensitivity of these seeds represents a problem for and long-term biodiversity conservation. This phenomenon raises questions as to how, where and why DS (desiccation sensitive)-seeded species appeared during evolution. These species evolved probably independently from desiccation tolerant (DT) seeded ancestors. They adapted to environments where the conditions are conducive to immediate germination after shedding, e.g. constant and abundant rainy seasons. These very predictable conditions offered a relaxed selection for desiccation tolerance that eventually got lost in DS seeds. These species are highly dependent on their environment to survive and they are seriously threatened by deforestation and climate change. Understanding of the ecology, evolution and molecular mechanisms associated with seed desiccation tolerance can shed light on the resilience of DS-seeded species and guide conservation efforts. In this review, we survey the available literature for ecological and physiological aspects of DS-seeded species and combine it with recent knowledge obtained from DT model species. This enables us to generate hypotheses concerning the evolution of DS-seeded species and their associated genetic alterations.

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Changes in Proline Levels during Seed Development of Orthodox and Recalcitrant Seeds of Genus Acer in a Climate Change Scenario

Forests ◽

10.3390/f11121362 ◽

2020 ◽

Vol 11 (12) ◽

pp. 1362

Author(s):

Joanna Kijowska-Oberc ◽

Aleksandra M. Staszak ◽

Mikołaj K. Wawrzyniak ◽

Ewelina Ratajczak

Keyword(s):

Climate Change ◽

Seed Development ◽

Desiccation Tolerance ◽

Seed Viability ◽

Climatic Conditions ◽

Proline Content ◽

Change Scenario ◽

Acer Pseudoplatanus ◽

Recalcitrant Seeds ◽

Seed Desiccation

In the present study, we examined the utility of proline usage as a biochemical indicator of metabolic changes caused by climate change (mean temperature and precipitation) during seed development of two Acer species differing in desiccation tolerance: Norway maple (Acer platanoides L.—desiccation tolerant—orthodox) and sycamore (Acer pseudoplatanus L.—desiccation sensitive—recalcitrant). In plants, proline is an element of the antioxidant system, which has a role in response to water loss and high temperatures. Our study considered whether proline could be treated as an indicator of tree seed viability, crucial for genetic resources conservation. Proline content was measured biweekly in developing seeds (between 11 and 23 weeks after flowering) collected in consecutive years (2017, 2018, and 2019). We showed that proline concentrations in recalcitrant seeds were positively correlated with mean two-week temperature. In contrast, in orthodox seeds no such relationship was found. Proline content proved to be sensitive to thermal-moisture conditions changes, which makes it a promising biochemical marker of seed desiccation tolerance in different climatic conditions.

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