Effects of source and level of dietary neutral detergent fiber on feed intake, ruminal fermentation, ruminal digestion in situ, and total tract digestion in beef cattle fed pelleted concentrates with or without supplemental roughage

1992 ◽  
Vol 70 (3) ◽  
pp. 884-893 ◽  
Author(s):  
S. A. Marshall ◽  
C. P. Campbell ◽  
I. B. Mandell ◽  
J. W. Wilton
2020 ◽  
Vol 33 (5) ◽  
pp. 763-769
Author(s):  
Thiwakorn Ampapon ◽  
Metha Wanapat

Objective: The experiment was conducted to study the effect of rambutan (Nephelium lappaceum) fruit peel powder (RP) on feed consumption, digestibility of nutrients, ruminal fermentation dynamics and microbial population in Thai breed cattle.Methods: Four, 2-year old (250±15 kg) beef bull crossbreds (75% Brahman×25% local breed) were allotted to experimental treatments using a 4×4 Latin square design. Four dietary supplementation treatments were imposed; non-supplementation (control, T1); supplementation of RP fed at 2% of dry matter intake (DMI) (low, T2); supplementation of RP fed at 4% of DMI (medium, T3) and supplementation of RP fed at 6% of DMI (high, T4). All cattle were given a concentrate supplement at 1% of body weight while Napier grass was provided as a free choice.Results: The findings revealed that RP supplementation did not negatively affect (p>0.05) DMI of Napier grass, while RP intake and total DMI were the greatest in the RP supplementation at 4% and 6% DMI. Nevertheless, the nutrients (dry matter, organic matter, crude protein, neutral detergent fiber, and acid detergent fiber) digestibilities were not changed in the RP supplementation groups. Rumen fermentation parameters especially those of total volatile fatty acids, acetate and butyrate were not significantly changed. However, the propionate concentration was remarkably increased (p<0.05) in the RP supplementation. Notably, the ratio of acetate to propionate, the number of protozoa, as well as the methane estimation were significantly reduced in the RP supplemented groups (4% and 6% of DMI), while the counts of bacteria was not altered.Conclusion: Supplementation of RP (4% of DMI) improved rumen propionate production, reduced protozoal population and methane estimation (p<0.05) without a negative effect on feed consumption and nutrients total tract digestibilities in beef cattle. Using dietary rambutan fruit peel powder has potential promise as a rumen regulator.


2020 ◽  
Vol 98 (5) ◽  
Author(s):  
Rodrigo S Goulart ◽  
Ricardo A M Vieira ◽  
Joao L P Daniel ◽  
Rafael C Amaral ◽  
Vanessa P Santos ◽  
...  

Abstract The objectives of this research were to evaluate the effects of source and concentration of α-amylase-treated neutral detergent fiber (aNDF) from roughage on feed intake, ingestive behavior, and ruminal kinetics in beef cattle receiving high-concentrate diets. Six ruminally cannulated Nellore steers (408 ± 12 kg of body weight) were randomly assigned to a 6 × 6 Latin square design with six diets: 10% aNDF from corn silage (10CS); 20% aNDF from corn silage (20CS); or four diets containing 10% aNDF from corn silage and 10% aNDF from one of the following sources: sugarcane (SC), sugarcane bagasse (SCB), soybean hulls (SH), or low oil cottonseed hulls (LOCH). The parameters of passage and degradation kinetics were estimated based on a two-compartmental model with gamma- and exponential-distributed residence times. The nonlinear models were fitted by nonlinear least squares, and a linear mixed-effects model was fitted to all variables measured from the Latin square design that were related to intake, digestibility, digestion kinetic parameters, and residence times. Mean particle size (MPS) between roughage sources (CS, SCB, and SC) and coproducts (SH and LOCH) was affected (P &lt; 0.05). Dry matter intake (DMI) was not affected (P &gt; 0.05) by 20CS, SC, SH, or LOCH. Steers fed 20CS or LOCH diets had 16% and 20% greater DMI, respectively, (P &lt; 0.05) than steers fed 10CS diet. Steers fed SCB consumed the least dry matter (DM). The SH and LOCH diets had lower MPS values (about 8.77 mm) in comparison to 20CS, SCB, and SC diets (about 13.08 mm) and, consequently, affected (P &lt; 0.05) rumen content, ruminal in situ disappearance, nutrient digestibility, and solid fractional passage rate. Chewing time was affected (P &lt; 0.05) by roughage sources and concentration. Lower values of distance travel inside the rumen (min/cm) were observed (P &lt; 0.05) for the SCB and SC diets in comparison with any other diet. Except for SCB, there was no difference (P &gt; 0.05) in rumen fill, among other treatments. Mean daily ruminal pH was not affected (P &gt; 0.05) by 20CS, SCB, SC, and LOCH diets, and it ranged from 6.1 to 6.23. Total short-chain fatty acids concentration was affected (P &lt; 0.05) by roughage source and concentration. Based on our results, we recommend that under Brazilian finishing diets, replacing roughage sources, except for SCB, based on aNDF concentration of the roughage in high-concentrate diets containing finely ground flint corn does not affect DMI.


2018 ◽  
Vol 102 (6) ◽  
pp. 1509-1514 ◽  
Author(s):  
Anusorn Cherdthong ◽  
Anuthida Seankamsorn ◽  
Chaichana Suriyapha ◽  
Pin Chanjula ◽  
Metha Wanapat

2020 ◽  
Vol 98 (8) ◽  
Author(s):  
Darren D Henry ◽  
Francine M Ciriaco ◽  
Rafael C Araujo ◽  
Pedro L P Fontes ◽  
Nicola Oosthuizen ◽  
...  

Abstract A replicated 5 × 5 Latin square design with a 2 × 2 + 1 factorial arrangement of treatments was used to determine the effects of bismuth subsalicylate (BSS) and encapsulated calcium ammonium nitrate (eCAN) on ruminal fermentation of beef cattle consuming bahiagrass hay (Paspalum notatum) and sugarcane molasses. Ten ruminally cannulated steers (n = 8; 461 ± 148 kg of body weight [BW]; average BW ± SD) and heifers (n = 2; 337 ± 74 kg of BW) were randomly assigned to one of five treatments as follows: 1) 2.7 g/kg of BW of molasses (NCTRL), 2) NCTRL + 182 mg/kg of BW of urea (U), 3) U + 58.4 mg/kg of BW of BSS (UB), 4) NCTRL + 538 mg/kg of BW of eCAN (NIT), and 5) NIT + 58.4 mg/kg of BW of BSS (NITB). With the exception of NCTRL, all treatments were isonitrogenous. Beginning on day 14 of each period, ruminal fluid was collected and rectal temperature was recorded 4× per day for 3 d to determine ruminal changes every 2 h from 0 to 22 h post-feeding. Ruminal gas cap samples were collected at 0, 3, 6, 9, and 12 h on day 0 of each period followed by 0 h on days 1, 2, 3, and 14. Microbial N flow was determined using Cr-Ethylenediaminetetraacetic acid, YbCl3, and indigestible neutral detergent fiber for liquid, small particle, and large particle phases, respectively. Data were analyzed using the MIXED procedure of SAS. Orthogonal contrasts were used to evaluate the effects of nonprotein nitrogen (NPN) inclusion, NPN source, BSS, and NPN source × BSS. There was no treatment effect (P &gt; 0.05) on concentrations of H2S on day 0, 1, 2, or 14; however, on day 3, concentrations of H2S were reduced (P = 0.018) when NPN was provided. No effect of treatment (P = 0.864) occurred for ruminal pH. There was an effect of NPN source on total concentrations of VFA (P = 0.011), where a 6% reduction occurred when eCAN was provided. There were effects of NPN (P = 0.001) and NPN source (P = 0.009) on the concentration of NH3-N, where cattle consuming NPN had a greater concentration than those not consuming NPN, and eCAN reduced the concentration compared with urea. Total concentrations of VFA and NH3-N were not affected (P &gt; 0.05) by BSS. There was an effect of BSS (P = 0.009) on rectal temperature, where cattle not consuming BSS had greater temperatures than those receiving BSS. No differences for NPN, NPN source, nor BSS (P &gt; 0.05) were observed for microbial N flow. In conclusion, eCAN does not appear to deliver equivalent ruminal fermentation parameters compared with urea, and BSS has limited effects on fermentation.


Animals ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 588 ◽  
Author(s):  
Trinidad de Evan ◽  
Andrea Vintimilla ◽  
Carlos N. Marcos ◽  
María José Ranilla ◽  
María Dolores Carro

The objective of this study was to analyze the chemical composition, in vitro ruminal fermentation, and intestinal digestibility of discarded samples of four Brassica vegetables: Brussels sprouts (BS), white cabbage, Savoy cabbage, and red cabbage, and to assess the effects of including increasing amounts of BS in the concentrate of a dairy sheep diet on in vitro fermentation, CH4 production, and in situ degradation of the diets. All cabbages had low dry matter content (DM; <16.5%), but their DM had high crude protein (19.5–24.8%) and sugars (27.2–41.4%) content and low neutral detergent fiber (17.5–28%) and was rapidly and extensively fermented in the rumen. Rumen degradability of protein at 12 h of in situ incubation was greater than 91.5% for all cabbages, and in vitro intestinal digestibility of protein ranged from 61.4 to 90.2%. Replacing barley, corn, and soybean meal by 24% of dried BS in the concentrate of a diet for dairy sheep (40:60 alfalfa hay:concentrate) increased in vitro diet fermentation and in situ degradability of DM and protein, and reduced in vitro CH4/total volatile fatty acid ratio. In vivo trials are necessary to confirm these results.


2006 ◽  
Vol 86 (3) ◽  
pp. 383-392 ◽  
Author(s):  
Y. Wang ◽  
B. P. Berg ◽  
L. R. Barbieri ◽  
D. M. Veira ◽  
T. A. McAllister

Grazing experiments were conducted over two years to evaluate the effects of incorporating sainfoin (Onobrychis viciifolia) into alfalfa (Medicago sativa) pasture on bloat, ruminal fermentation, and feed intake. Pastures of alfalfa (Alf) and alfalfa-sainfoin (AS) were established by cross-seeding (in 1998) or parallel-seeding in (1999) sainfoin into strip-seeded alfalfa. The pastures were used in grazing studies in 1999 and 2000. For periods of 4 or 5 d, 12 ruminally cannulated Jersey steers were turned out into pastures from 0830 to 1430 daily (without access to water) then were moved to a fenced area (with free access to water but no feed) until the next morning. Feed intake during grazing was estimated using alkane markers, development of bloat was assessed, and ruminal fluid (RF) fermentation properties were determined. Incidence of bloat on first-growth pastures grazed at early bloom in 1999 was unaffected by sainfoin (at 8.3%, DM basis) but was reduced by 80% (P < 0.001) in AS pasture compared with Alf, when grazed at full-bud in 2000 (12.1% sainfoin). With re-growth forage, bloat incidence was decreased by 77% (P < 0.05) on pasture containing 35% sainfoin, but treatment effect was not observed on pastures containing < 23% sainfoin. Incidence of bloat was reduced as the proportion of sainfoin in herbage mass increased. In 2000, ruminal ammonia concentrations were reduced (P < 0.05) with mixed forage containing ≥ 12.1% sainfoin, compared with Alf. Protozoal numbers, RF viscosity, and total volatile fatty acids (VFA) were unaffected by sainfoin in the forage, but acetate:propionate ratios were consistently reduced (P ≤ 0.036). Extracellular polysaccharide-degrading enzyme activities in RF were increased (carboxymethylcellulase and β-glucanase, P ≤ 0.034; xylanase, P = 0.008 and 0.08) by sainfoin at ≥ 12.1%, and proteolytic activities (intracellular and extracellular) were reduced (P ≤ 0.01). Enzyme activities were unaffected by sainfoin at ≤ 9.1%. Sainfoin did not affect (P ≥ 0.202) organic matter intake from any of the pastures surveyed. Sainfoin was more effective for reducing bloat when cattle were grazing first growth pastures than when grazing re-growth. Including 35% sainfoin in mixed alfalfa-sainfoin pastures reduced, but did not eliminate, bloat in beef cattle and enhanced protein utilization by decreasing proteolytic activity. Feed efficiency may be improved through associative effects of the two forages. Key words: Alfalfa, beef cattle, feed intake, grazing, pasture bloat, sainfoin


Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1247 ◽  
Author(s):  
Trinidad de Evan ◽  
Andrea Vintimilla ◽  
Eduarda Molina-Alcaide ◽  
María Ranilla ◽  
María Carro

The nutritive values for ruminants of cauliflower (CAU) and Romanesco (ROM) wastes (leaves, stems and sprouts) were assessed by analyzing their chemical composition, in vitro ruminal fermentation, and in vitro intestinal digestibility. In addition, the in vitro ruminal fermentation of diets containing increasing amounts of CAU was studied. The dry matter (DM) content of leaves, stems and sprouts of both vegetables was lower than 10%, but they contained high crude protein (CP; 19.9 to 33.0%) and sugar (16.3 to 28.7%) levels, and low neutral detergent fiber (21.6 to 32.3%). Stems and sprouts were more rapidly and extensively fermented in the rumen than leaves, but there were only minor differences the fermentation profiles of both vegetables. The estimated metabolizable energy content ranged from 9.3 (leaves) to 10.8 (sprouts) MJ/kg DM. The CP rumen degradability (12-h in situ incubations) was greater than 80.0% for all fractions, and the in vitro intestinal digestibility of CP ranged from 85.7 to 93.2%. The inclusion of up to 24% of dried CAU in the concentrate of a mixed diet (40:60 alfalfa hay:concentrate) increased the in vitro rumen fermentation of the CAU diet, but did not affect methane (CH4) production, indicating the lack of antimethanogenic compounds in CAU.


Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 172
Author(s):  
Kampanat Phesatcha ◽  
Krittika Chunwijitra ◽  
Burarat Phesatcha ◽  
Metha Wanapat ◽  
Anusorn Cherdthong

Urea–lime-treated rice straw fed to Thai native beef cattle was supplemented with dry yeast (DY) (Saccharomyces cerevisiae) to assess total feed intake, nutrient digestibility, rumen microorganisms, and methane (CH4) production. Sixteen Thai native beef cattle at 115 ± 10 kg live weight were divided into four groups that received DY supplementation at 0, 1, 2, and 3 g/hd/d using a randomized completely block design. All animals were fed concentrate mixture at 0.5% of body weight, with urea–lime-treated rice straw fed ad libitum. Supplementation with DY enhanced total feed intake and digestibility of neutral detergent fiber and acid detergent fiber (p < 0.05), but dry matter, organic matter and crude protein were similar among treatments (p > 0.05). Total volatile fatty acid (VFA) and propionic acid (C3) increased (p < 0.05) with 3 g/hd/d DY supplementation, while acetic acid (C2) and butyric acid (C4) decreased. Protozoal population and CH4 production in the rumen decreased as DY increased (p < 0.05). Populations of F. succinogenes and R. flavefaciens increased (p < 0.05), whereas methanogen population decreased with DY addition at 3 g/hd/d, while R. albus was stable (p > 0.05) throughout the treatments. Thus, addition of DY to cattle feed increased feed intake, rumen fermentation, and cellulolytic bacterial populations.


2010 ◽  
Vol 39 (7) ◽  
pp. 1571-1578 ◽  
Author(s):  
Regis Luis Missio ◽  
Ivan Luiz Brondani ◽  
Dari Celestino Alves Filho ◽  
Magali Floriano da Silveira ◽  
Leandro da Silva Freitas ◽  
...  

This study was developed to evaluate the ingestive behavior of beef cattle finished in feedlots with different concentrate levels in the diet. Sixteen bulls fed 22, 40, 59, or 79% of concentrate in the diet were assigned to a complete randomized design, with four replicates each. The supplied diets were isoproteic and the roughage used was corn silage. Data collection was carried out during the finishing period, with a total of six days. The time given to food intake, rumination, the number of bouts per meal and neutral detergent fiber rumination efficiency decreased linearly as the concentrate levels in the diet increased. Idle time increased linearly accordingly to the increment of concentrate in the diet. The animals have greater feed intake and rumination efficiency when fed higher levels of concentrate (79%).


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