scholarly journals Inflammaging: Role in pathogenesis and molecular diagnostics of age-related dilated cardiomyopathy

2021 ◽  
pp. 67-72
Author(s):  
И.М. Кветной ◽  
Н.С. Линькова ◽  
А.Э. Пухальская ◽  
К.Л. Козлов ◽  
Г.И. Гурко ◽  
...  
Keyword(s):  
Dilated Cardiomyopathy ◽  
Molecular Diagnostics ◽  
Molecular Medicine ◽  
Signal Molecules ◽  
Old People ◽  
Melatonin Synthesis ◽  
Age Related ◽  
Klotho Protein ◽  
Salivary Concentration ◽  
Elderly And Old People

Актуальность. В патогенезе дилатационной кардиомиопатии (ДКМП) у лиц старших возрастных групп важную роль играет инфламэйджинг и нарушение мелатонинобразующей функции эпифиза. Важной задачей молекулярной медицины является поиск сигнальных молекул - маркеров ДКМП. Цель работы - провести сравнительную оценку концентрации IL-1β, IL-6, Klotho, Sirt3, FGF23 и мелатонина в слюне у пациентов старших возрастных групп с ДКМП и без неё. Методы. Определение концентрации сигнальных молекул в слюне осуществляли методом иммуноферментного анализа с последующей статистической обработкой полученных данных. Результаты. У пациентов с ДКМП пожилого и старческого возраста концентрация маркеров инфламэйджинга (IL-1β, IL-6) в слюне повышалась в 8,6-19,1 раза по сравнению с этими показателями у лиц без ДКМП. Концентрация белка Klotho, ингибирующего синтез провоспалительных цитокинов, в слюне пациентов с ДКМП пожилого и старческого возраста была в 4,2-7,6 раза ниже по сравнению с этим показателем у лиц без ДКМП. У пациентов с ДКМП пожилого и старческого возраста концентрация в слюне мелатонина и регулируемого им Sirt3 была в 2,1-4,3 раза ниже по сравнению с этим показателем у лиц без ДКМП. Концентрация FGF23 в слюне не зависела от возраста пациентов и наличия ДКМП. Заключение. В патогенезе ДКМП у лиц старших возрастных групп важную роль играет инфламэйджинг. Об этом свидетельствует снижение синтеза белка Klotho и повышение уровня провоспалительных цитокинов IL-1β и IL-6. Другим звеном патогенеза ДКМП является снижение синтеза мелатонина и, как следствие, нарушение функции сигнального пути Mst1/Sirt3. Исследование концентрации IL-1β, IL-6, Klotho, SIRT3 и мелатонина в слюне может применяться для молекулярной диагностики ДКМП у лиц пожилого и старческого возраста. Background. Inflammaging and disorder of melatonin synthesis in the pineal gland play an important role in the pathogenesis of dilated cardiomyopathy (DC) in elderly and old people. An important objective of molecular medicine is searching for signal molecules, markers of DC. The aim of this work was to compare concentrations of IL-1β, IL-6, Klotho, Sirt3, FGF23, and melatonin in saliva of elderly and old DC patients and persons without DC. Methods. Concentrations of signal molecules were measured in saliva by ELISA. Results. Salivary concentrations of inflammaging markers (IL-1β, IL-6) in elderly and old DC patients were 8.6-19.1 times higher than in persons without DC. Salivary concentration of Klotho protein, an inhibitor of pro-inflammatory cytokine synthesis, was increased 4.2-7.6 times in elderly and old DC patients compared to the values in persons without DC. Salivary concentrations of melatonin and melatonin-regulated Sirt3 were decreased 2.1-4.3 times in elderly and old DC patients compared to the values in persons without DC. Salivary concentration of FGF23 did not depend on the age or presence of DC. Conclusion. Inflammaging plays in important role in the pathogenesis of DC in elderly and old people as evidenced by decreased synthesis of Klotho and increased levels of the pro-inflammatory cytokines IL-1β and IL-6. Another step in the DC pathogenesis is decreased melatonin synthesis and the resultant dysregulation of the Mst1/Sirt3 signaling way. Measuring concentrations of IL-1β, IL-6, Klotho, SIRT3, and melatonin in saliva can be used for molecular diagnostics of DC in elderly and old people.

scholarly journals INFLAMM-AGING: THE HYPOTHESIS OF THE INFLAMMATION ROLE IN ALZHEIMER DISEASE PROGRESS

2019 ◽  
Vol 19 (1S) ◽  
pp. 88-91
Author(s):  
I M Kventoy ◽  
N S Linkova ◽  
A S Diatlova ◽  
V A Zuev ◽  
T V Kventaia
Keyword(s):  
Comparative Analysis ◽  
Alzheimer Disease ◽  
Chronic Inflammation ◽  
Control Group ◽  
Signal Molecules ◽  
Old People ◽  
Age Related ◽  
Neurodegenerative Pathology ◽  
Τ Protein ◽  
Elderly And Old People

Inflamm-aging - the term, describes the development of chronic inflammation during aging without the infection pathology. It is supposed, that inflamm-aging is one of the reason of age-related pathology, partially, Alzheimer disease (AD). There were done comparative analysis of AD (Аβ42, τ-protein, р16) and inflammation (IL-6, TGFα, NF-kB) markers in hippocamp and blood lymphocytes in elderly and old people. It was shown, that expression of investigated signal molecules in hippocamp and lymphocytes of elderly and old AD people increased in comparison with people of control group (without neurodegenerative pathology). Thus, inflammation mediators play important role in AD pathogenesis and can be the potential target for neuropathology therapy.

THE INFLUENCE OF POLYPEPTIDE LIVER COMPLEX AND TETRAPEPTIDE ON ORGANISM PHYSIOLOGICAL FUNCTION IN NORM AND AGE-RELATED PATHOLOGY

2020 ◽  
pp. 159-164
Author(s):  
Б. И. Кузник ◽  
Н. В. Хасанова ◽  
Г. А. Рыжак ◽  
И. Е. Мещерякова ◽  
В. Х. Хавинсон
Keyword(s):  
Chronic Hepatitis ◽  
Antioxidant Status ◽  
High Efficiency ◽  
Physiological Function ◽  
Experimental Models ◽  
Old People ◽  
Age Related ◽  
Acute And Chronic Hepatitis ◽  
Elderly And Old People

Применение большого числа лекарственных средств у лиц пожилого и старческого возраста часто приводит к нарушению функции печени. При этом с возрастом повышается риск развития цирроза печени, острого и хронического гепатита, что обусловливает необходимость поиска эффективных и безопасных гепатопротекторов. В обзоре приведены данные исследования гепатопротекторных, иммуномодулирующих и геропротекторных свойств полипептидного комплекса, экстрагированного из печени телят (Вентвил) и тетрапептида KEDA ( Lys-Glu-Asp-Ala , Ливаген). В моделях экспериментальной патологии печени у животных (острый и хронический гепатит, цирроз) и исследованиях in vitro показана высокая эффективность Вентвила и пептида KEDA . Вентвил и пептид KEDA обладают сходными свойствами - приводят к норме иммунный и антиоксидантный статус, восстанавливают функции печени при гепатите. Показано, что наибольшее гепато-и иммунопротекторное действие пептидов проявляется при старении. The applying of many drugs in elderly and old people often is the reason of liver dysfunction. Thereat, the risk of liver fibroid induration, acute and chronic hepatitis increases during aging. It is the reason to find new, effective and harmless hepatoprotectors. In the review is shown the data of hepatoprotective, immunoprotective and antiageing properties of liver polypeptide complex (Ventvil) and KEDA tetrapeptide ( Lys-Glu-Asp-Ala , Livagen). In liver pathology experimental models (liver fibroid induration, acute and chronic hepatitis) in amimals and in vitro was shown high efficiency of Ventvil and KEDA peptide. Ventvil and KEDA peptide had concordant effects - normalized immune and antioxidant status, restored liver function during hepatitis. It was demonstrated, that maximal hepato- and immunoprotective effect of peptides verified in aging.

What Can We Learn from Sarcopenia with Curarisation in the Context of Cancer Surgery? A Review of the Literature

2019 ◽  
Vol 25 (28) ◽  
pp. 3005-3010
Author(s):  
Georges Samouri ◽  
Alexandre Stouffs ◽  
Lionel V. Essen ◽  
Olivier Simonet ◽  
Marc De Kock ◽  
...  
Keyword(s):  
Frail Elderly ◽  
Muscle Fibers ◽  
Motor Units ◽  
The Elderly ◽  
Hepatic Function ◽  
Volume Of Distribution ◽  
Neuromuscular Blocking ◽  
Cancer Surgery ◽  
Old People ◽  
Age Related

Introduction: The monitoring of the curarisation is a unique opportunity to investigate the function of the neuromuscular junction (NMJ) during cancer surgery, especially in frailty-induced and age-related sarcopenia. Method: We conducted a comprehensive literature review in PubMed, without any limit of time related to frailty, sarcopenia, age and response to neuromuscular blockers in the context of cancer surgery. Results: Several modifications appear with age: changes in cardiac output, a decrease in muscle mass and increase in body fat, the deterioration in renal and hepatic function, the plasma clearance and the volume of distribution in elderly are smaller. These changes can be exacerbated in cancer patients. We also find modifications of the NMJ: dysfunctional mitochondria, modifications in the innervation of muscle fibers and motor units, uncoupling of the excitation-contraction of muscle fibers, inflammation. : Neuromuscular blocking agents (NMBAs) compete with acetylcholine and prevent it from fixing itself on its receptor. Many publications reported guidelines for using NMBAs in the elderly, based on studies comparing old people with young people. : No one screened frailty before, and thus, no studies compared frail elderly and non-frail elderly undergoing cancer surgery. Conclusion: Despite many studies about curarisation in the specific populations, and many arguments for a potential interest for investigation, no studies investigated specifically the response to NMBAs in regard of the frailty-induced and age-related sarcopenia.

scholarly journals Absence of Thrombospondin-2 Causes Age-Related Dilated Cardiomyopathy

Circulation ◽  
2009 ◽  
Vol 120 (16) ◽  
pp. 1585-1597 ◽  
Author(s):  
Melissa Swinnen ◽  
Davy Vanhoutte ◽  
Geert C. Van Almen ◽  
Nazha Hamdani ◽  
Mark W.M. Schellings ◽  
...  
Keyword(s):  
Dilated Cardiomyopathy ◽  
Age Related

scholarly journals Aptamers for Detection and Diagnostics (ADD): Can mobile systems linked to biosensors support molecular diagnostics of SARS-CoV-2? Should molecular medicine explore multiple alternatives as adjuvants to or replacement for traditional and non-traditional vaccines?

2021 ◽  
Author(s):  
Shoumen Datta
Keyword(s):  
Data Sharing ◽  
Molecular Diagnostics ◽  
Low Cost ◽  
Mobile App ◽  
Mobile Systems ◽  
Molecular Medicine ◽  
Invasive Monitoring ◽  
Non Invasive ◽  
Secure Information ◽  
Biomedical Device

Proposed SARS-CoV-2 surveillance tool using a mobile app for non-invasive monitoring of humans and animals. <p>Engineering a biomedical device as a low-cost, non-invasive, detection, and diagnostic platform for surveillance of infections in humans, and animals. The system embraces the IoT <i>“digital by design”</i> metaphor by incorporating elements of connectivity, data sharing and (secure) information arbitrage. Using an array of aptamers to bind viral targets may help in detection, diagnostics, and potentially prevention in case of SARS-CoV-2. The ADD tool may become part of a broader platform approach.</p>

scholarly journals CURRENT CONCEPTUAL APPROACHES FOR ASSESSMENT OF THE PROCESS OF AGEING AND OLD AGE

2017 ◽  
Vol 5 ◽  
pp. 722-727
Author(s):  
Vanina Mihaylova ◽  
Dimitar Shopov ◽  
Iliya Bivolarski ◽  
Adolf Alakidi ◽  
Kristina Kilova
Keyword(s):  
Old Age ◽  
21St Century ◽  
Positive Attitude ◽  
Well Being ◽  
Good Health ◽  
Old People ◽  
Active Approach ◽  
Quality Life ◽  
Long Life ◽  
Elderly And Old People

: Ageing should be considered not only as an increase in the number of elderly and old people in their absolute and relative numbers, but also as a unity of the transformations of the lifecycle, with an emphasis on: later retirement, prolonged period of good‑quality life, an active approach towards the process of retirement and differentiation of the category of “fourth age”. The general preparation for old age has earned a new appeal in the contemporary societies. Motivation of the old people for activity – both physical and intellectual – is of great importance for the better survival of the old age and long life in good health, supported by realized well-being and feeling of joy from life. In this aspect the study of both risk and protective factors for human health (in a salutogenetic perspective) becomes increasingly fundamental. The issue of population at an advanced age has definitely been considered as an independent subject since the beginning of the 21st century. Moreover, the society perceives it as an essential basis for further progress and flourishing of the mankind. In this sense the demographic strategies treating the problem need to address it in a new positive way, with different and positive attitude, accepting the population-related failures and anxiety and turning them into challenges and advantages.

scholarly journals Dementia in elderly patients as a consequence of arterial hypertension (literature review)

2021 ◽  
Author(s):  
Sergey S Mikhailov ◽  
Kirill L. Kozlov ◽  
Andrey N Shishkevich ◽  
Evgeniy Yu. Bessonov ◽  
Nikolai G. Lukjanov ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Arterial Hypertension ◽  
Brain Function ◽  
Middle Age ◽  
The Elderly ◽  
World Population ◽  
Old People ◽  
Aged People ◽  
Number Of Patients ◽  
Elderly And Old People

Alzheimer's disease and vascular dementia are important causes of cognitive decline in the elderly and senile. This problem is becoming increasingly important in connection with the general aging of the world population and an increase in the number of patients with concomitant pathology, among which arterial hypertension plays an important role. There are a number of studies proving the link between arterial hypertension in middle age and dementia in the elderly. It is hoped that the normalization of blood pressure among middle-aged people will reduce the incidence of cognitive dysfunction. It is also known that maintaining blood pressure at acceptable levels in elderly and old people can improve brain function and reduce the prevalence of dementia, but it is not entirely clear which blood pressure numbers are optimal.

Downregulation of Klotho expression by dehydration

2011 ◽  
Vol 301 (4) ◽  
pp. F745-F750 ◽  
Author(s):  
Cai Tang ◽  
Ganesh Pathare ◽  
Diana Michael ◽  
Abul Fajol ◽  
Melanie Eichenmüller ◽  
...  
Keyword(s):  
Transmembrane Protein ◽  
Accelerated Aging ◽  
Early Death ◽  
Renal Tissue ◽  
Hek293 Cells ◽  
Protein Abundance ◽  
Transcript Levels ◽  
Age Related ◽  
Klotho Protein ◽  
Growth Deficit

Klotho, a transmembrane protein, protease, and hormone mainly expressed in renal tissue counteracts aging. Overexpression of Klotho substantially prolongs the life span. Klotho deficiency leads to excessive formation of 1,25(OH)2D3, growth deficit, accelerated aging, and early death. Aging is frequently paralleled by dehydration, which is considered to accelerate the development of age-related disorders. The present study explored the possibility that dehydration influences Klotho expression. Klotho transcript levels were determined by RT-PCR, and Klotho protein abundance was detected by Western blotting in renal tissue from hydrated and 36-h-dehydrated mice as well as in human embryonic kidney (HEK293) cells. Dehydration was followed by a significant decline of renal Klotho transcript levels and protein abundance, accompanied by an increase in plasma osmolarity as well as plasma ADH, aldosterone, and 1,25(OH)2D3 levels. Antidiuretic hormone (ADH; 50 nM) and aldosterone (1 μM) significantly decreased Klotho transcription and protein expression in HEK293 cells. In conclusion, the present observations disclose a powerful effect of dehydration on Klotho expression, an effect at least partially mediated by enhanced release of ADH and aldosterone.

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