Uranium concentration variation dependency on gender correlated with age of bladder cancer patient

Human health was and still the most important problem and objective of all most researches. Finding out what causes in the decadence of healthiness of Iraqi population is our tendency in the present work, Uranium causing cancer that is affected by a correlation between age and gender of bladder cancer patients is studied in the present work. Mean of Uranium concentration (Uc) decreased with increasing age for all age group without dependency on gender. While, there is a wide dispersion in Mean Uc excretion between males and females, due to the effect of correlated gender with age, where female Mean Uc is maximum at age 50-69 year (2.355 µg/L), and it's higher than male Mean Uc (2.022 µg/L) in this age stage because of menopause, also average period of illness and the percentage of patients are affected by correlated gender with age. So that factor of gender correlation with age affects in the calculation of background levels and radiation exposure and causing bladder cancer incidence.

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Association Between Cervical Abrasion and Age and Its Influence on Gender - A Retrospective Study

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl3.2961 ◽

2020 ◽

Vol 11 (SPL3) ◽

pp. 441-446

Author(s):

Nur Liyana Hannah Binti Izham Akmal ◽

Adimulapu Hima Sandeep ◽

Revathi Duraisamy

Keyword(s):

Retrospective Study ◽

Mechanical Forces ◽

Age Group ◽

Cervical Lesions ◽

Age And Gender ◽

Tooth Structure ◽

High Prevalence ◽

Males And Females ◽

The Difference ◽

And Gender

Cervical lesions are often characterized by defects seen in the gingival third of a tooth crown which may be in the facial or lingual surface. Pathological loss of tooth structure caused by factors other than dental caries such as cervical abrasion is referred to as non-carious cervical lesions (NCCL). Cervical abrasion is an example of NCCL in which a constant exposure of the tooth to mechanical forces leads to pathological wearing away of the hard tissues. In most of the cases, cervical tooth lesions are revealed to be more common as the age increases. Several studies have reported the difference in the prevalence of cervical abrasion between males and females. Many reports suggest that cervical abrasion is commonly associated with improper tooth brushing habits. To evaluate the prevalence of cervical abrasion between genders and its influence on age. It is a retrospective study conducted using the case records of Saveetha Dental College and Hospital, Chennai, India from June 2019 to March 2020. Data including the patient’s name, patient’s identification number (PID), age, gender and presence of cervical abrasion were retrieved from the patients’ case records. A total of 742 consecutive case records were retrieved and analysed. Cervical abrasion was observed in 371 individuals of this study. High prevalence of cervical abrasions was seen in males (70.9%) compared to females (29.1%). Most of the cases were observed in individuals within the 41-50 years age group (28.8%), and the least was seen within the 11-20 years age group (0.3%). Within the limits of the study, most of the cervical abrasion cases are recorded in individuals within the 41-50 years age group with higher predilection in males. There is a statistically significant association of cervical abrasion with age and gender.

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Urine cell flow cytometry analysis of PD-L1 expression and DNA content for bladder cancer.

Journal of Clinical Oncology ◽

10.1200/jco.2019.37.7_suppl.466 ◽

2019 ◽

Vol 37 (7_suppl) ◽

pp. 466-466

Author(s):

Pin-I Chen ◽

Alice (Xiaoyang) Wang ◽

Mustafa Deebajah ◽

Shaheen Alanee ◽

Bruce Kendrick Patterson

Keyword(s):

Cell Cycle ◽

Flow Cytometry ◽

Bladder Cancer ◽

Epithelial Cells ◽

Cancer Patient ◽

Cancer Patients ◽

Dna Content ◽

Urine Samples ◽

Normal Donor ◽

Bladder Cancer Patient

466 Background: Bladder cancer is the fifth most common cancer in the United States. PD-1/PD-L1, a pathway used by cancer cells to evade immune response, correlates with bladder cancer severity and has emerged as a target in bladder cancer treatment. Chromosomal instability is also a prominent feature associated with the development of bladder cancer. A method to unbiasedly analyze PD-L1 expression and DNA content in cells from urine samples will help us better understand bladder cancer. Methods: To evaluate the PD-L1 expression and DNA content, we developed a 4-color flow assay. Cells in urine samples were pelleted, fixed/permeabilized (in incellMAX, IncellDx Inc.) and stained with antibodies against pan-cytokeratin (CK), CD45, PD-L1 and a cell cycle dye. The stained samples were analyzed by a flow cytometer alongside stained control cells. Results: Fifty bladder cancer patient and 15 normal donor urine samples were collected and tested with this assay. We could distinguish epithelial cells (pan-CK+) and white blood cells (WBCs, CD45+) in urine samples and obtain PD-L1 expression and DNA content information simultaneously from these cell populations. The patient samples showed a significantly higher percentage of WBCs with substantial PD-L1 expression. The percentage of PD-L1 positive epithelial cells was not distinguishable between normal donor and patient samples. However, increased post G1 epithelial cells ( > 5%) were observed in a majority of bladder cancer patients, with around 25% of samples showing a DNA index above 1.05. In addition, a comparison of urine collection fixatives showed that incellMAX-fixed samples had the best single cell recovery and DNA content measurement, as shown by lower cell cycle dye staining variability (lower rCV). Statistically significant differences were found between cancer patients and normal samples. Conclusions: We developed a flow cytometry-based method to investigate PD-L1 and DNA content simultaneously in cells from urine samples. Comparing urine samples from bladder cancer patients and normal yielded statistically significant differences that could provide valuable information for bladder cancer patient management.

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Exploration of the TMB and hypermutation landscape in Chinese pan-cancer patient by next-generation sequencing.

Journal of Clinical Oncology ◽

10.1200/jco.2021.39.15_suppl.e14575 ◽

2021 ◽

Vol 39 (15_suppl) ◽

pp. e14575-e14575

Author(s):

Shuo Wang ◽

Jiasheng Xu ◽

Jian Sun ◽

Deng Wei ◽

Xinsheng Zhang ◽

...

Keyword(s):

Cancer Patient ◽

Cancer Patients ◽

Age And Gender ◽

Tumor Mutation Burden ◽

Mutation Burden ◽

Cancer Types ◽

And Gender ◽

Chinese Cancer Patients ◽

Pan Cancer ◽

Generation Sequencing

e14575 Background: Among a variety of malignant tumors, the level of the patient's TMB was currently an important criterion for clinical judgment whether to adopt immunotherapy. Hypermutation could produce many nearby mutation sites at the same time, which seriously damages genetic material and may cause cancer. Therefore, performing TMB detection on cancer patients and understanding the occurrence of hypermutation in pan-cancer patients will help clinical researchers to further understand the disease characteristics of cancer patients and helped the choice of treatment methods. Previously, TMB and Hypermutation had been tested and studied in pan-cancer patients in the United States and Europe, but rare research was reported in China. In this study, we explored the TMB and hypermutation landscape in Chinese pan-cancer patient by next-generation sequencing. Methods: A total of 8,361 cancer patients from multiple cancer hospitals and research centers in China were included in the study. We sequenced 8,361 Chinese cancer patients from 8 cancer types using the oncopanscan product of Genetron Health Co., Ltd. and calculated the tumor mutation burden of the patients. We separately analyzed the tumor mutation burden of patients in 8 cancer types and analyzed the relationship between the occurrence of hypermutation and the patient's age and gender. Results: The results showed that in pan-cancer, hypermutation patients accounted for 16.97%, and ultrahypermutation patients accounted for 0.78%. Among them, patients with lung cancer have the highest proportion of hypermutation, reaching 27.72%, and patients with colorectal cancer have the highest proportion of ultrahypermutation, reaching 2.86%. Correlation analysis between TMB and age and gender was carried out on 8336 patients. The results showed that in the patients with intrahepatic bile duct cancer, the proportion of men and women was the same. Among the other cancer types, hypermutation patients were more male, and the proportion of men with liver cancer was the largest, with 90 percent. We further explored the correlation between the TMB of pan-cancer patients and the patient’s age, and found that in gastric cancer, liver cancer, and melanoma, the older patients have higher TMB; however, the younger the patients in brain cancer, the higher the TMB ( P<0.05). Conclusions: In this study, we explored the TMB and hypermutation landscape in Chinese pan-Cancer patient for the first time. We found that among Chinese cancer patients, lung cancer patients have the highest proportion of hypermutation. In a variety of cancers, hypermutation patients account for a higher proportion of men, and the older the patient, the higher the TMB.

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82 Single-cell RNA sequencing and CITE-Seq analysis of bladder cancer patient urine with matched tumor and peripheral blood suggests urine as a window into the tumor immune microenvironment

Journal for ImmunoTherapy of Cancer ◽

10.1136/jitc-2021-sitc2021.082 ◽

2021 ◽

Vol 9 (Suppl 3) ◽

pp. A90-A90

Author(s):

Michelle Tran ◽

Adam Farkas ◽

Kristin Beaumont ◽

Timothy O’Donnell ◽

Reza Mehrazin ◽

...

Keyword(s):

Bladder Cancer ◽

Cancer Patient ◽

Single Cell ◽

Cancer Patients ◽

Immune Cells ◽

Peripheral Blood ◽

Single Cells ◽

Bladder Cancer Patient ◽

Immune Microenvironment ◽

Tumor Immune Microenvironment

BackgroundFDA-approved immunotherapies for early and advanced stage bladder cancer have response rates of 15–65% in bladder cancer, suggesting that tumor-associated resistance mechanisms undermine their efficacy. Accordingly, there is an unmet need to identify accessible biomarkers that predict response. Urine, which is in direct contact with urothelial tumors, represents an easily accessible patient material that may reflect cellular and/or genetic signatures related to immune resistance. It has been demonstrated that urine from bladder cancer patients contains not only tumor cells, which are routinely assessed by clinical urinalyses, but also immune cells that previous studies suggest may reflect the tumor microenvironment (TME).1 However, the concordance between cells in the urine and those in bladder tumors is unknown., Here, we characterized patient urine in an unbiased fashion by performing the first single-cell RNA sequencing (scRNAseq) and Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) on matched bladder cancer patient urine, tumor, and peripheral blood.MethodsMatched tumor tissue, urine, and peripheral blood were collected from bladder cancer patients (n=7) during surgery; either trans-urethral resection of bladder tumor or cystectomy. All three tissues were processed to single-cell suspensions and sequenced using the 10X Genomics platform (scRNAseq: 17 samples, CITE-seq: 3 samples). These sequencing approaches permitted quantification of both transcriptomic and surface protein expression of 54,469 cells total.2 3 Analysis was performed using Seurat, Enrichr, and Monocle packages and platforms.4 5 6Results scRNAseq of urine from bladder cancer patients revealed several immune populations including CD4+ and CD8+ T cells, Treg cells, NK cells, B cells, neutrophils, dendritic cells, monocytes, and macrophages in addition to non-hematopoietic lineages including bladder epithelial cells, neuronal cells, prostate epithelial cells, fibroblasts, myofibroblasts, and endothelial cells. The composition and transcriptional profiles of urine immune cells were more similar to TME immune cells than to peripheral blood immune cells. Urine immune cells expressed gene signatures associated with hypoxia, anergy, pro-inflammation, and glucose deprivation that were more similar to tumor immune cells than those in the peripheral blood.ConclusionsOur work represents the first scRNAseq and CITEseq profiling of cancer patient urine. Our study suggests several viable immune cells shed in bladder cancer patient urine that look more transcriptionally and phenotypically similar to the TME than peripheral blood cells. This important finding has several implications for future research and clinical applications as urine can be sampled non-invasively in scenarios when tumor resection may not be feasible.ReferencesWong YNS, Joshi K, Khetrapal P, et al. Urine-derived lymphocytes as a non-invasive measure of the bladder tumor immune microenvironment. Journal of Experimental Medicine. 2018; 215:2748–59.Zheng GXY, Terry JM, Belgrader P, et al. Massively parallel digital transcriptional profiling of single cells. Nature Communications 2017; 8.Stoeckius M, Hafemeister C, Stephenson W, et al. Simultaneous epitope and transcriptome measurement in single cells. Nature Methods 2017;14, 865–68.Butler A, Hoffman P, Smibert, P, et al. Integrating single-cell transcriptomic data across different conditions, technologies, and species. Nature Biotechnology 2018; 36: 411–20.Xie Z, Bailey A, Kuleshov MV, et al. Gene set knowledge discovery with Enrichr. Current Protocols 2021.Trapnell C, Cacchiarelli D, Grimsby J, et al. The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells. Nature Biotechnology 2014; 32: 381–6.Ethics ApprovalThe study was approved by Mount Sinai Institution’s Ethics Board, approval number 10–1180. Participants gave informed consent before taking part in the study.

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Morphometric Study of Pituitary Gland with Correlation of Age and Gender using Magnetic Resonance Imaging

Birat Journal of Health Sciences ◽

10.3126/bjhs.v6i2.40322 ◽

2021 ◽

Vol 6 (2) ◽

pp. 1476-1480

Author(s):

Sunima Maskey ◽

Dil Islam Mansur ◽

Subindra Karki ◽

Pragya Shrestha

Keyword(s):

Pituitary Gland ◽

Transverse Dimension ◽

Mean Value ◽

The Body ◽

Age Group ◽

Age And Gender ◽

Significant Difference ◽

Males And Females ◽

The Mean ◽

And Gender

Introduction: The pituitary gland is the master gland of the body. It’s size varies with age and gender. MRI is the safest and effective diagnostic tool for pituitary gland examination. Objectives: This study was aim to determine the anteroposterior, height and transverse dimensions of normal pituitary gland in different age groups of both sex with MRI. Methodology: It was a descriptive cross-sectional study. It consisted of 567 images of individuals (242 males and 325 females) of various age from 20 to 70 years from the Department of Radio- diagnosis, Dhulikhel Hospital. Results: The mean anteroposterior, height and transverse dimension of pituitary gland is 9.74±1.18 mm, 5.95±1.11 mm and 11.65±2.15 mm respectively in which females have higher value. Independent sample t test showed highly significant differences (p< 0.05) between the mean anteroposterior dimension in males and females. The present study showed the mean value of anteroposterior dimension is maximum at age group 50-59. The mean value of height, transverse dimension and volume of males and females is maximum at 20-29 age group and minimum at 70-79 age group. One way ANOVA shows that there is significant difference between in height, transverse dimension and volume at different age group (p< 0.05). Conclusion: It was concluded that the height and volume of pituitary gland is maximum at second decade of life then it gradually decreases with age. The mean value of anteroposterior, height and transverse dimension showed greater value of females than males.

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