scholarly journals Derivatised liquid chroamtographic method for statistical estimation of saliva samples by non-invasive technique

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1240-1243
Author(s):  
Vasundhara S ◽  
Madhusudan Rao Bandlamudi
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Glucose Level ◽  
Sample Volume ◽  
Invasive Technique ◽  
Array Detector ◽  
P Value ◽  
Glucose Levels ◽  
Non Invasive ◽  
Short Period

A method was developed and validated for low-level detection of glucose. The method involves quantitation of glucose though derivitisation with PABA and HPLC-DAD analysis. A selective and novel method has been optimised for evaluation of blood glucose levels in blood Serum and saliva biological matrices by R.P-H.P.L.C. The principle analyte was eluted with the conditions of mobile phase having the 50m.M Sodium acetate: Acetonitrile (60:40%, v./v.) using the Phenomax-C.18  (250 x 4.6 mm, 3.5m.) analytical column with the 1.0 ml/min flow rate and 10µl sample volume at 254 nm in a photodiode array detector. The retention times of was 3.4 min within the total run time of 05 min. The curve indicates the correlation coefficient (r2) was superior by having the value 0.998 with a linear range of 40 µg/ml- 600.0 µg/ml. Based on the results obtained in the validation, the developed method was susceptible, accurate, linear and economical. Due to the short time of the chromatographic program, more samples can be analysed within a short period.  Data obtained were subjected to statistical analysis using t-test. Highly significant P-value (P<0.05) was obtained between the serum blood glucose and salivary glucose level. A distinct difference was observed in the salivary glucose between the control and diabetic group. The method was met all the predefined acceptance criteria.  Diabetes mellitus is a globally widespread disease. As the salivary collection is painless and non-invasive, in this study, an attempt has been made to diagnose diabetes mellitus by estimating the salivary glucose level in comparison with serum blood glucose level. Hence the developed method can be used as an index of diabetes mellitus.

scholarly journals Assessment of the salivary glucose as a non-invasive test for diabetic patients

2019 ◽  
Vol 6 (6) ◽  
pp. 2289
Author(s):  
Kajol Thapa ◽  
Saroj Kunwar ◽  
Sonu Thapa ◽  
Asmita Phuyal ◽  
Sahil Rupakheti
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Diabetic Patients ◽  
Glucose Levels ◽  
Non Invasive ◽  
Invasive Test ◽  
Blood Glucose Levels ◽  
Institutional Review

Background: Over the recent year there has been a startling rise in the number of people suffering from diabetes mellitus. Estimation of blood glucose levels has been an essential laboratory investigation for screening and monitoring of diabetes. Saliva is one of the secretions in human body whose collection is easy and non-invasive. Salivary glucose hence could serve as an easy and non-invasive tool.Methods: Institutional review committee of NHRC gave us permission to carry out this study. 105 subjects with Type 2 diabetes mellitus who attended the OPD at Star hospital, Sanepa, Lalitpur, Nepal and 106 healthy individuals were consented to participate in this study. Glucose was measured by the GOD-POD (Glucose oxidase peroxidase) methods using the semi-autoanalyser and salivary glucose was compared with corresponding blood glucose levels.Results: A significant positive correlation of fasting salivary glucose level and fasting blood glucose level was observed in healthy (r=0.241, p=0.001) and in diabetic patients (r=0.202, p=0.001).Conclusions: The study implies a potential for saliva in monitoring and screening of diabetes mellitus.

scholarly journals Non Invasive Estimation of Glucose by Monitoring Pancreas using NIR Sensor

2019 ◽  
Vol 8 (3) ◽  
pp. 5651-5653
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Glucose Level ◽  
Metabolic Disorders ◽  
Biological Molecules ◽  
Glucose Levels ◽  
Non Invasive ◽  
Blood Glucose Levels ◽  
The Brain

To ensure normal body function, control of blood glucose levels is necessary. A network of various hormones and neuropeptides assure blood glucose. Hormones freed from the brain, pancreas, liver, intestine help to assure glucose level. The pancreas represents a major role in secreting the blood sugar-lowering hormone insulin and its opponent glucagon. However, disturbances in the hormones and peptides involved lead to metabolic disorders such as type 2 diabetes mellitus (T2DM).It is essential to understand the mechanisms underlying the various interactions of biological molecules with blood. Many methods help to measure glucose concentration. To measure the blood glucose level, a new method is proposed through the nanotechnology

scholarly journals Influence of Nutrition Education with Calendar Method in Diabetic Patients' Blood Glucose

2018 ◽  
Vol 3 (2) ◽  
pp. 72
Author(s):  
Setyoadi Setyoadi ◽  
Heri Kristianto ◽  
Siti Nur Afifah
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Nutrition Education ◽  
Community Health Center ◽  
Control Group ◽  
P Value ◽  
Diabetic Patients ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Before And After

Diabetes mellitus is a disease that required good self-management. Noncompliance in diet and meal plans cause the instability of blood glucose levels. Nutrition education calendar method can improve knowledge and ability to consume food that matches the number, hours and types with dietary adjustments listed in the calendar diet. This study aimed to determine the effect of nutrition education calendar method on blood glucose levels of patients with type 2 diabetes mellitus in Community Health Center Pakis Malang. Pre-experimental design one group pretest-posttest with purposive sampling was conducted in this study and sample obtained as many as 21 people. Blood glucose levels were measured before and after the nutrition education calendar methods. Compliance in using calendar method with the observation sheet. Statistical analysis values obtained by Wilcoxon, the p-value of 0.007 (p <0.05). The results of the analysis, 16 respondents showed a decrease in blood glucose levels and 5 respondents experienced an increase in blood glucose levels after the given intervention. It can be concluded that there are differences between blood glucose levels before and after nutrition education calendar method. Differences in blood glucose levels can be influenced by controlling diet respondent in accordance with the calendar method in education, but also antidiabetic drugs, and sports. Should be added to the control group to determine objectively the effect of nutrition education on the calendar method.

scholarly journals Comparison of Functional Regression and Functional Principal Component Regression for Estimating Non-Invasive Blood Glucose Level

2021 ◽  
Vol 5 (1) ◽  
pp. 14-25
Author(s):  
Nurul Fadhilah ◽  
Erfiani Erfiani ◽  
Indahwati Indahwati
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Principal Component Regression ◽  
Principal Component ◽  
Functional Regression ◽  
Glucose Levels ◽  
Non Invasive ◽  
Blood Glucose Levels ◽  
Functional Principal Component

The calibration method is an alternative method that can be used to analyze the relationship between invasive and non-invasive blood glucose levels. Calibration modeling generally has a large dimension and contains multicolinearities because usually in functional data the number of independent variables (p) is greater than the number of observations (p>n). Both problems can be overcome using Functional Regression (FR) and Functional Principal Component Regression (FPCR). FPCR is based on Principal Component Analysis (PCA). In FPCR, the data is transformed using a polynomial basis before data reduction. This research tried to model the equations of spectral calibration of voltage value excreted by non-invasive blood glucose level monitoring devices to predict blood glucose using FR and FPCR. This study aimed to determine the best calibration model for measuring non-invasive blood glucose levels with the FR and FPCR. The results of this research showed that the FR model had a bigger coefficient determination (R2) value and lower Root Mean Square Error (RMSE) and Root Mean Square Error Prediction (RMSEP) value than the FPCR model, which was 12.9%, 5.417, and 5.727 respectively. Overall, the calibration modeling with the FR model is the best model for estimate blood glucose level compared to the FPCR model.

scholarly journals EFFECT OF AEROBIC EXERCISES AND RESISTANCE TRAINING ON GLUCOSE LEVELS IN TYPE 2 DIABETIC SUBJECTS.

2017 ◽  
Vol 10 (7) ◽  
pp. 360
Author(s):  
Aishwarya Pramod Benkar ◽  
Smita Bhimrao Kanase
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Resistance Training ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Postprandial Blood Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Significant Difference

Objective: Diabetes mellitus is a leading cause of death and disability in the world and its prevalence is predicted to rise to 10% by 2030. Hence, this study is conducted with objectives to find out the effect of aerobic exercises and resisted exercises on blood glucose levels in type 2 diabetes mellitus (T2DM) subjects and to compare the effect of both exercises on blood glucose level.Method: The comparative study was conducted at Krishna Institute of Medical Sciences Deemed University, Physiotherapy department, Karad. 30 participants with age group between 30 and 65 years were taken. Subjects were selected as per inclusion and exclusion criteria. Group A (15) participants were given aerobic exercise on static bicycle, and Group B (15) participants were given resistance training using dumbbells and weight cuffs for 5 days/week for 4 weeks. Diet recommendations were given to every participant.Results: Statistical analysis was performed using paired and unpaired t-test. Analysis showed statistically extremely significant difference in fasting blood glucose level and postprandial blood glucose level in both the groups (p≤0.0001).Conclusion: Thus, this study concludes that both aerobic exercises and resistance training prove to be beneficial in controlling blood glucose levels in T2DM subjects.

scholarly journals Correlation of Salivary Glucose level with Blood Glucose Level in Patients with Diabetes Mellitus

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1410-1414
Author(s):  
Gnanamoorthy Kothai ◽  
Acksa Alex ◽  
Aruna Bholenath Patil ◽  
Athanallur Raman Malathy ◽  
Prasanna Karthik Suthakaran
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Glucose Level ◽  
Venous Blood ◽  
Fasting Blood Glucose ◽  
Glycemic Status ◽  
Regular Monitoring ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
The Individual

Diabetes Mellitus (DM) is a systemic disorder characterized by hyperglycemia either due to insulin resistance or insulin deficiency. This can lead to many serious life-threatening complications if not managed properly by regular monitoring of glycemic status. Prevalence of fear of needles in the society make people non-compliant to regular monitoring. Thus, there is a need for a non-invasive method for determining the glycemic status of the individual. Salivary Glucose has the potential to be one such tool. This study aimed to find whether a correlation between fasting blood glucose levels and fasting salivary glucose levels could be established in diabetic and non-diabetic individuals. 50 patients with DM and 50 patients without DM were studied. 5 ml of venous blood and 5 ml of unstimulated saliva after overnight fasting were collected from each participant and processed using standardized enzymatic methods. The data was analyzed using SPSS software. There was a strong and very significant positive correlation (r=0.800, p=0.001) between fasting salivary glucose levels and fasting blood glucose levels in patients with DM whereas the correlation was weak and insignificant in patients without DM (r=0.111, p=0.441). The cut off value for diagnosing DM was found to be ˃ 2.2mg/dl with 100% specificity and 100% sensitivity.

scholarly journals Gambaran kadar glukosa darah puasa pada pekerja kantor

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Louis E. Ugahari ◽  
Yanti M. Mewo ◽  
Stefana H.M. Kaligis
Keyword(s):  
Physical Activity ◽  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Sedentary Lifestyle ◽  
Fasting Blood Glucose ◽  
Office Workers ◽  
Glucose Levels ◽  
Blood Glucose Levels

Abstract: Blood glucose must be maintained in a constant concentration. Hyperglycemia, an increase of blood glucose level, can be a symptom of diabetes mellitus. One of the factors that affect the blood glucose level is physical activity. Low physical activity can be influenced by work. Sedentary lifestyle is identic with office workes. Office workers do not enough time to do physical activity. This study was aimed to describe the fasting blood glucose level among office workers. This was a descriptive study with a cross sectional design. Respondents were obtained by using total sampling method. There were 52 respondents in this study consisted of 25 males and 27 females. The results showed that 45 respondents (86.54%) had normal fasting blood glucose levels, 5 respondents (9.62%) had high fasting blood glucose levels (hyperglycemia), and 2 respondents (3.84%) had low fasting blood glucose levels (hypoglycemia). The maximum value was 243 mg/dL, the minimum value was 63 mg/dL, the median value was 83 mg/dL, the average value was 94.42 mg/dL, and standard of deviation was 37.85 mg/dL. Conclusion: Most office workers had normal blood glucose levels.Keywords: fasting blood glucose, office workers Abstrak: Glukosa darah dalam tubuh manusia harus dijaga dalam konsentrasi yang konstan. Kadar glukosa darah dalam tubuh yang meningkat (hiperglikemia) dapat menjadi gejala penyakit diabetes mellitus. Salah satu faktor yang memengaruhi kadar glukosa darah ialah aktivitas fisik. Aktivitas fisik yang kurang dapat dipengaruhi oleh pekerjaan. Pola hidup sedentary lifestyle identik dengan pekerja kantor dewasa ini. Pekerja kantor tidak memiliki waktu untuk terlibat dalam aktivitas fisik yang cukup. Penelitian ini bertujuan untuk mengetahui gambaran kadar glukosa darah puasa pada pekerja kantor. Jenis penelitian ialah deskriptif dengan desain potong lintang. Pemilihan responden dilakukan dengan metode total sampling. Responden terdiri dari 25 orang laki-laki s dan 27 orang perempuan. Hasil penelitian mendapatkan 45 responden (86,54%) memiliki kadar glukosa darah puasa normal, 5 responden (9,62%) memilki kadar glukosa darah puasa tinggi (hiperglikemia), dan 2 responden (3,84%) memilki kadar glukosa puasa rendah (hipoglikemia). Hasil pengukuran kadar glukosa darah puasa mendapatkan nilai maksimum 243 mg/dL, nilai minimum 63 mg/dL, nilai median 83 mg/dL, nilai rata-rata 94,42 mg/dL dan standar devisiasi 37,85 mg/dL. Simpulan: Sebagian besar pekerja kantor masih memiliki kadar glukosa darah puasa yang normal. Kata kunci: glukosa darah puasa, pekerja kantor

scholarly journals GIVING RED RICE (ORIZA NIVARA) TO CHANGE GLUCOSE LEVELS IN DIABETES MELLITUS PATIENTS IN KENDARI CITY

2021 ◽  
Vol 3 (1) ◽  
pp. 38-44
Author(s):  
La Ode Ardiansyah ◽  
Nawawi Nawawi
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Brown Rice ◽  
Secondary Data ◽  
The Body ◽  
P Value ◽  
Red Rice ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Quasi Experimental

Background:Diabetes mellitus (DM) is a phenomenon which the body  cannot  produce insulin supply which is indicated by increasing blood glucose levels. Red Rice (Oriza nivara) contains flavonoid compounds which have antidiabetic abilities. It can  reduce blood glucose by increasing insulin secretion and preventing insulin resistance. This study aims to determine the effect of giving brown rice (Oriza nivara) to change   blood glucose levels in people with diabetes mellitus in Kendari City. Methods: This research method was carried out with a quasi-experimental design with pre-post test design for 1 week with primary and secondary data collection techniques. The respondents  were  determined by proportional random sampling. Respondents in this study were 33 people with DM. Result:The results of the study on checking blood glucose levels before and after giving brown rice (oriza nivara) within 1 week obtained P value = 0.000 <  alpha 0.05. Conclusion: there is a  significant  effect of giving brown rice (oriza nivara) on changes in blood glucose level in diabetes mellitus people  in Kendari City.

scholarly journals Biochemical markers in saliva of patients with diabetes mellitus

2012 ◽  
Vol 59 (4) ◽  
pp. 198-204 ◽  
Author(s):  
Biljana Andjelski-Radicevic ◽  
Radica Dozic ◽  
Tatjana Todorovic ◽  
Ivan Dozic
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Diabetic Patients ◽  
Non Invasive ◽  
Stress Markers ◽  
Frequent Monitoring ◽  
Patients With Diabetes ◽  
Protein Components

Diabetes mellitus is metabolic syndrome characterized by disorder in metabolism of carbon hydrates, lipids and proteins. The diagnosis of diabetes is established by measuring the blood glucose level using standardized methods. Frequent monitoring of blood glucose level could be inconvenient for patients because of possible pain during blood sample taking. In the last few years biological materials with non invasive sampling, like saliva, have been analyzed. Research has confirmed that some organic and inorganic components of saliva are modified in diabetic patients (glucose, lipid and protein components, oxidative stress markers, electrolytes). Beside other markers, the analysis of glucose in saliva is an attempt to find a non-invasive and painless way for frequent monitoring of glucose concentration in diabetic patients. Collecting saliva is simple and economical, it neither requires expensive equipment nor specially trained staff. Saliva can be taken many times and in unlimited quantity. In regards to the data about the possibilities for using saliva as biological sample in monitoring diabetes mellitus, which could be alternative to blood serum or plasma, the conclusion is that saliva becomes more important in this context.

scholarly journals Diabetic Foot Spa Implementation in Early Neuropathy Diagnosis Based on Blood Glucose Levels, Foot Sensitivity and the Ankle Brachial Index in Patients with Diabetes Mellitus

Jurnal NERS ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 106
Author(s):  
Erika Martining Wardani ◽  
Chilyatiz Zahroh ◽  
Nur Ainiyah
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Diabetic Foot ◽  
Ankle Brachial Index ◽  
Vascular Complications ◽  
P Value ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Patients With Diabetes ◽  
Foot Problems

Introduction: Diabetes Mellitus (DM) is a cause of morbidity mainly due to vascular complications. The prevention of diabetic foot problems can be done through metabolic control and foot exercises. The purpose of this study was to determine the effect of diabetes foot spa measures on blood glucose levels, foot sensitivity and the ankle brachial index.Methods: This research was a pre-experimental design. The population totaled 170 DM patients and the sample was finalized at 30 respondents. The independent variable was diabetic foot spa, while the dependent variables were blood glucose levels, blood sensitivity and the ankle brachial index. The data was analyzed using a t-test and Wilcoxon rank test.Results: Diabetic foot spa in early neuropathy detection testing has a blood glucose level of t=9.523 and a p value=0.000. Diabetic foot spa also significantly affects foot sensitivity with a ρ <α (0.001<0.05) and an ankle brachial index of (ABI) ρ<α (0.008 < 0.05).Conclusion: Diabetic foot spa conducted regularly and independently can reduce the level of complications in Diabetes Mellitus patients. Furthermore, the tingling and pain in the feet can be reduced or even disappear as well as preventing complications such as foot ulcers that can often become amputations.

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