scholarly journals Effects of paroxetine on biochemical parameters and reproductive function in male rats

2021 ◽  
Vol 12 (4) ◽  
pp. 2308-2315
Author(s):  
Rachid Mosbah ◽  
Aziez Chettoum ◽  
Alberto Mantovani
Keyword(s):  
Biochemical Parameters ◽  
Selective Serotonin Reuptake Inhibitors ◽  
Semen Quality ◽  
Sperm Count ◽  
Reproductive Function ◽  
Reproductive Organs ◽  
Male Rats ◽  
Sperm Viability ◽  
Male Wistar Rats ◽  
Paroxetine Treatment

Selective serotonin reuptake inhibitors (SSRI) are a class of molecules used in treating depression, anxiety, and mood disorders. Paroxetine (PRT) is one of the most prescribed antidepressants, which has attracted great attention regarding its side effects in recent years.  This study was planned to assess the adverse effects of paroxetine on the biochemical parameters and reproductive system. Fourteen male Wistar rats were randomly allocated into two groups (7 rats for each): control and treated with paroxetine at a dose of 5mg/kg.bw for two weeks. At the end of the experiment, blood was collected from the retro-orbital plexus for measuring the biochemical parameters, whereas the reproductive organs were removed for measuring semen quality and the histological investigations. Results showed that paroxetine induced significant changes in some biochemical parameters and alteration of semen quality, including sperm count, spermatids number and sperm viability, motility and abnormalities. The histopathological examinations of testis and epididymis revealed an alteration of spermatogenesis, cellular disorganization and vacuolization, enlargement of interstitial space, shrinkage and degenerative changes in the epithelium of seminiferous and epididymal tubules with few to nil numbers of spermatozoa in their lumen. In conclusion, paroxetine treatment caused changes in some biochemical parameters and sperm profiles as well as histopathologic effects of reproductive organs.

Aflatoxin B1-Induced Reproductive Toxicity in Male Rats

2014 ◽  
Vol 33 (3) ◽  
pp. 155-161 ◽  
Author(s):  
Ch. Supriya ◽  
B. P. Girish ◽  
P. Sreenivasula Reddy
Keyword(s):  
Aflatoxin B1 ◽  
Sperm Count ◽  
Reproductive Toxicity ◽  
Serum Testosterone ◽  
Reproductive Organs ◽  
Docking Studies ◽  
Male Rats ◽  
In Silico Docking ◽  
Male Wistar Rats ◽  
Steroidogenic Acute Regulatory

Aflatoxin B1 (AFB1), one of the most common mycotoxins found in human foods, is principally hepatotoxic; however, it also affects reproduction. The aim of the present study was to elucidate the reproductive toxic effects and possible mechanism of action of AFB1 in rats. Male Wistar rats were injected intramuscularly with doses of 10, 20, or 50 µg AFB1/kg body weight on alternate days from 45 to 100 days of age. Significant reductions in body weights, relative weights of reproductive organs, daily sperm production, epididymal sperm count, viable sperm, motile sperm, and hypoosmotic swelling-tail coiled sperm were observed. Significant decreases in testicular steroidogenic enzymes and serum testosterone levels were also observed indicating decreased steroidogenesis. In silico docking studies illustrated AFB1 binds with steroidogenic acute regulatory (StAR) protein thereby affecting the transport of cholesterol into mitochondria resulting in decreased steroidogenesis.

scholarly journals Effect of Croton caudatus Geiseler Aqueous Root Extract on Reproductive and Biochemical Parameters in Male Wistar Rats

2020 ◽  
Vol 12 (3) ◽  
pp. 251-60
Author(s):  
Razif Dasiman ◽  
Mastura Abd Malek ◽  
Ebby Anuar Bahari ◽  
Fatin Nadzirah Zakaria ◽  
Nina Keterina Hashim ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Sperm Count ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Computer Assisted ◽  
Root Extract ◽  
Rheumatic Arthritis ◽  
Male Wistar Rats ◽  
Histological Results ◽  
Aqueous Root Extract

BACKGROUND: Croton caudatus Geiseler (CCG), a local herb that empirically has been used as traditional medicine for malaria, fever, convulsions, and rheumatic arthritis. This study aimed to investigate the effect of CCG on reproductive and biochemical parameters in male Wistar rats.METHODS: Twenty-four Wistar male rats were divided randomly into four groups, which were treated with CCG aqueous root extract at the concentration of 0-16 mg/kg, via oral gavage. After 21 days of treatment, blood, sperm, and testes were harvested for analyses. Sperm parameters were evaluated using computer-assisted sperm analyser (Hamilton Thorne Sperm Analyzer), and morphometric analyses of histological changes were performed.RESULTS: CCG extract at 8 and 16 mg/kg significantly (p<0.05) increased the total sperm count, concentration, and motility; whereby, only 16 mg/kg significantly decreased the percentage of abnormal sperm morphology. Testosterone hormones levels were the highest in 4 mg/kg CCG but were slightly decreased in 8 and 16 mg/kg CCG. The histological results showed a significant increase in the germinal epithelial height, decrease of lumen size at 16 mg/kg of CCG extract. No significant changes of alanine aminotransferase (ALT) and creatinine levels in serum from all CCG extract groups. However, the aspartate aminotransferase (AST) level in 8 mg/kg CCG was elevated, and the blood urea nitrogen (BUN) level was decreased in all CCG extract groups.CONCLUSION: In conclusion, the CCG extract at 4 mg/kg has the potential to enhance spermatogenesis activity within the seminiferous tubules in rats without any hepatotoxicity and nephrotoxicity effect on the liver and kidney.KEYWORDS: Croton caudatus Geiseler, rats, spermatogenesis, seminiferous tubules, testis, testosterone

scholarly journals Reproductive disorders in male rats induced by high-fructose consumption from juvenile age to puberty

2020 ◽  
Vol 71 (1) ◽  
pp. 78-86
Author(s):  
Oleksandr Y. Tkachenko ◽  
Ganna M. Shayakhmetova ◽  
Anatoliy V. Matvienko ◽  
Valentina M. Kovalenko
Keyword(s):  
Sperm Count ◽  
Reproductive Function ◽  
Treated Group ◽  
Male Rats ◽  
Reproductive Disorders ◽  
Male Reproductive Function ◽  
Fructose Intake ◽  
High Fructose ◽  
Male Wistar Rats ◽  
Post Implantation

AbstractThere is compelling evidence that a hypercaloric, high-fructose diet can cause metabolic syndrome (MetS) and a whole range of other metabolic changes. In the context of androgen deficiency, MetS in boys merits special attention, but the effects of fructose-rich diet in youth on future male reproductive function are still poorly evidenced. The aim of this study was to address this issue and analyse the effects of high-fructose intake starting from weaning to puberty (postnatal day 23 up to 83) on the reproductive function of male rats. For this purpose juvenile male Wistar rats were divided in two groups: control and the group receiving 10 % fructose solution instead of drinking water. Reproductive function was evaluated in terms of fertility, sperm count, testes/epididymis morphology, and serum sex hormones. The fructose-treated group showed a decrease in testosterone and twofold increase in luteinising and follicle-stimulating hormone levels in the serum. This was accompanied with lower testis/epididymis weights, sperm count, and changed testis/epididymis morphology. Their fertility remained unchanged, but the fertility of females mating with these males diminished. In addition, pre-implantation and post-implantation embryonic death rate rose in these females. Our results have confirmed that high fructose consumption from early age until puberty can impair the reproductive function of male rats, and call for further animal and epidemiological investigation.

Effects of paroxitine on biochemical parameters and reproductive function in male rats

2019 ◽  
Author(s):  
Rachid Mosbah ◽  
Aziz Chettoum ◽  
Zohir Djerrou ◽  
Alberto Mantovani
Keyword(s):  
Biochemical Parameters ◽  
Reproductive Function ◽  
Male Rats

scholarly journals Effect of Tramadol on Sperm Profile of Male Albino Rats

2019 ◽  
pp. 1-6
Author(s):  
I. S. Esua ◽  
U. U. Uno ◽  
U. B. Ekaluo
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Sperm Head ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Sperm Viability ◽  
Albino Rat ◽  
Significant Difference

Background and Aim: Tramadol is a potent analgesic effective in the treatment of mild to severe pains. However, the use of the drug can pose a threat to other organs and systems. Therefore, this study evaluated the effect of graded doses of tramadol on sperm profile of male albino rats. Materials and Methods: Eighteen male rats were divided into three groups (A, B and C) using completely randomized design (CRD) with six rats in each group. Rats in group A served as the control group and were given just food and water while groups B and C were given tramadol at 50 and 100 mg/kg body weight (BW) respectively, daily for the period of 65 days. The treatment was administered via oral gavage and at the end of the treatments, the rats were sacrificed. Immediately after sacrifice, a puncture was made in the epididymis with a sterile pin and examined for semen pH. The epididymes were processed for epididymal sperm motility, viability, count and sperm head abnormality. Results: There was no significant difference in the weight of testes and semen pH. Sperm viability, sperm motility, sperm count and weight of epididymes significantly reduced (p<0.05) in tramadol treated animals when compared with the control. Results also indicated statistically significant (p<0.05) increase in sperm head abnormalities in rats treated with tramadol when compared with the control. Conclusion: The results obtained from this study reveal that tramadol has negative effects on weight of epididymes, sperm count, sperm viability, sperm motility and sperm head abnormalities in male albino rat as mammalian models in a dose dependent manner.

scholarly journals Effect of Phyllanthus amarus on Some Reproductive Indices of Male Albino Rats

2019 ◽  
pp. 1-8
Author(s):  
P. B. Ekpo ◽  
N. E. Edu ◽  
A. J. Umoyen ◽  
T. L. Thomas ◽  
S. O. Abraham
Keyword(s):  
Medicinal Plants ◽  
Sperm Count ◽  
Reproductive Organs ◽  
Male Rats ◽  
Albino Rats ◽  
Phyllanthus Amarus ◽  
Experimental Animals ◽  
Treatment Groups ◽  
Randomized Design ◽  
Group B

Background: Medicinal plants have been a good source of drugs for humans, but chronic and prolong use of medicinal plants like Phyllanthus amarus for the treatment of malaria and other disorders are issues of concerns. This study evaluated the effect of Phyllanthus amarus on reproductive organs and sperm parameters in albino rats. Materials and Methods: Twenty-four healthy male albino rats of 12 weeks old were assigned into four groups with six rats in each group using a Completely Randomized Design (CRD). The experimental animals were orally treated with Phyllanthus amarus. Group A served as the control and was given only water and feed; Group B, C and D  received 100 mg/kgBW, 200 mg/kgBW and 300 mg/kgBW of Phyllanthus amarus respectively. Data obtained were analyzed using analysis of variance (ANOVA). The treatments lasted for a period of 65 days after two weeks of acclimatization. Results: The results showed statistically significant (p<0.05) reduction in weight of testes and epididymes, sperm motility, sperm viability, sperm count and sperm head abnormalities in male  rats treated with Phyllanthus amarus when compared to the control. The sperm pH was not significantly (p>0.05) affected by Phyllanthus amarus among the different treatment groups in the experimental animals. Conclusion: Findings from the present study indicate that Phyllanthus amarus possesses a dose-dependent anti-fertility activity in amale albino rats under a sub-chronic course of administration.

scholarly journals Effect of epigallocatechin gallate on aluminum chloride-induced changes in behavior, biochemical parameters, and spermatogenesis of Sprague-Dawley rats

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Subramani Parasuraman ◽  
Brenda Ngu Yen Qin ◽  
Lam Chew Hui ◽  
James Yu Kar Beng
Keyword(s):  
Vitamin C ◽  
Aluminum Chloride ◽  
Biochemical Parameters ◽  
Sperm Count ◽  
Epigallocatechin Gallate ◽  
Biochemical Changes ◽  
Male Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Induced Changes

Abstract Background Epigallocatechin gallate (EGCG) acts as an antioxidant by preventing oxidative stress. The effect of EGCG on aluminum-induced testicular injury is not clear. Hence, the present study is planned to investigate the effect of EGCG on aluminum chloride (AlCl3)-induced changes in behavior, biochemical parameters, and spermatogenesis in male Sprague-Dawley rats. The rats were divided into six groups with six animals each. All the animals were administered with respective assigned treatment once daily for 28 days. The animals in groups I to VI were administered with drug vehicle, AlCl3, vitamin C, EGCG, vitamin C, and EGCG, respectively. The animals in groups V and VI were additionally challenged with AlCl3 (10 mg/kg) immediately after vitamin C and EGCG administration, respectively. Changes in behavior were measured on day 1, 14 and 28. At the end of the study, the blood sample was collected from all the animals, and the serum was separated and used for biochemical analysis. Later, the rats were subjected to bilateral orchiectomy; sperm was collected from the cauda epididymis for microscopic examination. Then, the animals were sacrificed, and the organs such as the brain, lungs, heart, liver, kidney, spleen, and testis were collected for organ weight analysis. Results The animal administered with AlCl3 showed a reduction in locomotor activity, grip strength, and escape latency time whereas vitamin C prevented the effect of AlCl3. But, EGCG did not show any significant changes in AlCl3-induced behavioral and biochemical changes. At the end of the study, vitamin C prevented AlCl3-induced behavioral and biochemical changes. The group of animals administered with AlCl3 showed a reduction in the number of spermatozoa whereas AlCl3 + vitamin C and AlCl3 + EGCG did not show any significant changes in the number of spermatozoa when compared to the control group. Conclusion EGCG prevented AlCl3-induced reduction in epididymal sperm count of male rats and did not show any significant effect on AlCl3-induced changes in behavior and biochemical parameters, whereas vitamin C had an ameliorative effect on AlCl3-induced changes in behavior, biochemical parameter, and spermatogenesis. Graphical abstract

Reproductive dysfunction after mercury exposure at low levels: evidence for a role of glutathione peroxidase (GPx) 1 and GPx4 in male rats

2017 ◽  
Vol 29 (9) ◽  
pp. 1803 ◽  
Author(s):  
Caroline S. Martinez ◽  
Franck M. Peçanha ◽  
Daniela S. Brum ◽  
Francielli W. Santos ◽  
Jeferson L. Franco ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Reproductive Toxicity ◽  
Membrane Integrity ◽  
Reproductive Function ◽  
Reproductive Organs ◽  
Mercury Contamination ◽  
Male Rats ◽  
Mercury Chloride ◽  
Male Reproductive Function

Mercury is a ubiquitous environmental pollutant and mercury contamination and toxicity are serious hazards to human health. Some studies have shown that mercury impairs male reproductive function, but less is known about its effects following exposure at low doses and the possible mechanisms underlying its toxicity. Herein we show that exposure of rats to mercury chloride for 30 days (first dose 4.6 µg kg–1, subsequent doses 0.07 µg kg–1 day–1) resulted in mean (± s.e.m.) blood mercury concentrations of 6.8 ± 0.3 ng mL–1, similar to that found in human blood after occupational exposure or released from removal of amalgam fillings. Even at these low concentrations, mercury was deposited in reproductive organs (testis, epididymis and prostate), impaired sperm membrane integrity, reduced the number of mature spermatozoa and, in the testes, promoted disorganisation, empty spaces and loss of germinal epithelium. Mercury increased levels of reactive oxygen species and the expression of glutathione peroxidase (GPx) 1 and GPx4. These results suggest that the toxic effects of mercury on the male reproductive system are due to its accumulation in reproductive organs and that the glutathione system is its potential target. The data also suggest, for the first time, a possible role of the selenoproteins GPx1 and GPx4 in the reproductive toxicity of mercury chloride.

scholarly journals RANKL regulates male reproductive function

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Martin Blomberg Jensen ◽  
Christine Hjorth Andreassen ◽  
Anne Jørgensen ◽  
John Erik Nielsen ◽  
Li Juel Mortensen ◽  
...  
Keyword(s):  
Seminal Fluid ◽  
Semen Quality ◽  
Sperm Count ◽  
Predictive Biomarkers ◽  
Wild Type Mouse ◽  
Reproductive Function ◽  
Human Testis ◽  
Male Reproductive Function ◽  
Infertile Men ◽  
Sperm Counts

AbstractInfertile men have few treatment options. Here, we demonstrate that the transmembrane receptor activator of NF-kB ligand (RANKL) signaling system is active in mouse and human testis. RANKL is highly expressed in Sertoli cells and signals through RANK, expressed in most germ cells, whereas the RANKL-inhibitor osteoprotegerin (OPG) is expressed in germ and peritubular cells. OPG treatment increases wild-type mouse sperm counts, and mice with global or Sertoli-specific genetic suppression of Rankl have increased male fertility and sperm counts. Moreover, RANKL levels in seminal fluid are high and distinguishes normal from infertile men with higher specificity than total sperm count. In infertile men, one dose of Denosumab decreases RANKL seminal fluid concentration and increases serum Inhibin-B and anti-Müllerian-hormone levels, but semen quality only in a subgroup. This translational study suggests that RANKL is a regulator of male reproductive function, however, predictive biomarkers for treatment-outcome requires further investigation in placebo-controlled studies.

scholarly journals Beneficial Role of Ginger Powder (Zingiber officinale) against Acephate-induced Reprotoxicity in Adult Male Rats

2021 ◽  
pp. 50-59
Author(s):  
V. Madhavi ◽  
U. Kanchana Ganga ◽  
S. B. Sainath ◽  
B. Kishori
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Sperm Count ◽  
Membrane Integrity ◽  
Male Rats ◽  
Sperm Viability ◽  
Marker Enzymes ◽  
Group I ◽  
Sperm Membrane

Aims: The present study was aimed to investigate the protective role of ginger against acephate-induced testicular toxicity in adult rats. Methodology: Rats were allocated into four groups where animals in group I served as controls, while animals in group II, III and group IV were treated as experimental rats. Rats in groups II, III and IV were treated with acephate (50mg/kg body weight), ginger (100mg/kg body weight) and combination of both acephate and ginger, respectively over a period of 60 days. After completion of experimental period sperm count, sperm viability, sperm motility, sperm membrane integrity, testicular steroidogenic marker enzymes (3β-HSD and 17β-HSD, serum testosterone and testicular architecture was performed in both control and experimental rats. Results: Relative weights of reproductive organs, sperm count, sperm viability, sperm motility and sperm membrane integrity were significantly decreased in acephate treated rats over controls. Acephate administration also reduced the circulatory levels of testosterone associated with a significant reduction in the testicular steroidogenic marker enzymes (3β-HSD and 17β-HSD) in rats. The testicular architecture was disrupted in acephate intoxicated rats. In contrast, ginger administration significantly recovered the acephate-induced suppressed selected reproductive parameters with increased circulatory levels of testosterone and restoration of sperm endpoints in as compared to acephate alone treated rats. No significant changes were observed in any of the selected reproductive endpoints in ginger treated rats as compared to controls. Conclusion: The results can be concluded that supplementation of ginger mitigates the negative effects of acephate on male reproductive health via amelioration of testicular setroidogenesis and spermatogenesis and epididymal sperm maturation events in rats.

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