scholarly journals Effect of Tramadol on Sperm Profile of Male Albino Rats

2019 ◽  
pp. 1-6
Author(s):  
I. S. Esua ◽  
U. U. Uno ◽  
U. B. Ekaluo
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Sperm Head ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Sperm Viability ◽  
Albino Rat ◽  
Significant Difference

Background and Aim: Tramadol is a potent analgesic effective in the treatment of mild to severe pains. However, the use of the drug can pose a threat to other organs and systems. Therefore, this study evaluated the effect of graded doses of tramadol on sperm profile of male albino rats. Materials and Methods: Eighteen male rats were divided into three groups (A, B and C) using completely randomized design (CRD) with six rats in each group. Rats in group A served as the control group and were given just food and water while groups B and C were given tramadol at 50 and 100 mg/kg body weight (BW) respectively, daily for the period of 65 days. The treatment was administered via oral gavage and at the end of the treatments, the rats were sacrificed. Immediately after sacrifice, a puncture was made in the epididymis with a sterile pin and examined for semen pH. The epididymes were processed for epididymal sperm motility, viability, count and sperm head abnormality. Results: There was no significant difference in the weight of testes and semen pH. Sperm viability, sperm motility, sperm count and weight of epididymes significantly reduced (p<0.05) in tramadol treated animals when compared with the control. Results also indicated statistically significant (p<0.05) increase in sperm head abnormalities in rats treated with tramadol when compared with the control. Conclusion: The results obtained from this study reveal that tramadol has negative effects on weight of epididymes, sperm count, sperm viability, sperm motility and sperm head abnormalities in male albino rat as mammalian models in a dose dependent manner.

The Impact of Gemcitabine-induced Reproductive Toxicity on The Sperm count and Morphology of Albino Rats

2021 ◽  
Vol 12 (1) ◽  
pp. 357-365
Author(s):  
Bassam Abdulaziz Alahmadi
Keyword(s):  
Reproductive System ◽  
Sperm Count ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Human Dose ◽  
Significant Difference ◽  
Recovery Group ◽  
Sperm Count And Morphology ◽  
The Impact

Cancer treatments can affect sperm production and a significant percentage of cancer patients may develop permanent azoospermia or severe oligozoospermia after chemotherapy. To investigate the influence of Gemcitabine toxicity on the reproductive system of albino male rats (sperm count and morphology). An experimental animal study conducted in the zoology department, College of Science, King Saud University during the period from June to October 2014 using albino rats (Rattusnorvegicus) (Wistar strain). Males were divided into four different groups (control" 0 mg/kg",7 mg/kg,14 mg/kg, and 21 mg/kg). The reproductive organs, testicles and epididymis decreased in weight and atrophied in most of the animals treated with the drug in various doses. The mean absolute and relative epididymal weights were also significantly decreased. In the drug-effects recovery group, neither the testicles nor the epididymis in the animals treated with the three doses recovered fully normal weight. The testis's efficiency in producing sperm was significantly decreased at all doses. In the recovery group, the testis regained its efficiency, as no significant difference was recorded between the drug-treated groups and the control group. The drug caused complete loss of sperm, in a rat treated with the big dose. Gemcitabine caused a significant increase in the percentage of deformed sperms in all treated animals. Gemcitabine drug has high toxicity on the reproductive system of rats with a dose tenth of human dose, with a massive decrease in the count and quantity of sperm, which means that this drug can have more toxicity effects on human.

scholarly journals The Effect of Single and Triple Testicular Biopsy Using Biopty Gun on Spermatogenesis in Pubertal Rats

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1569
Author(s):  
Tomislav Šušnjar ◽  
Ivana Kuzmić Prusac ◽  
Ivan Švagelj ◽  
Anđela Jurišić ◽  
Tomislav Šušnjar ◽  
...  
Keyword(s):  
Sperm Count ◽  
Semen Analysis ◽  
Study Groups ◽  
Testicular Biopsy ◽  
Male Rats ◽  
Lower Percentage ◽  
Control Group ◽  
Sprague Dawley ◽  
Left Testis ◽  
Significant Difference

Background: The aim of this study was to compare consequences in single and triple testicular biopsy by biopty gun in pubertal rats using histological and immunohistochemical analysis. Methods: Thirty-two Sprague-Dawley male rats were used as the experimental model. The rats were randomly divided into three study groups. The rats from the first group (n = 12) received a single-biopsy of upper pole of the left testis, while the rats from the second group (n = 10) received triple-biopsy of upper and lower poles and lateral surface of left testis. The third group (n = 10) was a control group. On the eightieth day after the biopsy in all rats bilateral orchiectomy and funiculectomy were performed to obtain testicular tissue and sperm for analysis. The consequences of the puncture were observed by pathohistology, immunohistochemistry and semen analysis. Results: The results of the study showed lower percentage of sperm count (14.5 mill/mL vs. 16 mill/mL, p = 0.130), sperm motility (24.6% vs. 32.7%, p > 0.05), abnormal sperm (30% vs. 27%, p > 0.05), atrophic tubules (21% vs. 6%, p < 0.001), volume (1.7 mL vs. 2.28 mL, p < 0.01) and apoptotic index (1.56 vs. 1.19, p = 0.650) in the testes with a triple-biopsy compared to the testes with a single-biopsy. Semen analysis showed a borderline significant difference between the group with triple-biopsy where sperm count was lower than it in the control group (14.5 mill/mL vs. 17.5 mill/mL, p = 0.05). A single-biopsy has little effect on the testis, especially on overall fertility. A triple-biopsy showed higher degree of the testicular damage but without a significant impact on overall fertility. Semen analysis showed that single- and triple-biopsies did not have a significant effect on sperm count, motility and morphology. Conclusion: Biopty gun procedure is a cheap, simple and reliable method for testicular biopsy in rats without a significant effect on sperm count, motility and morphology.

Impact of supplementing commercial rabbit pellets with fresh Moringa oleifera leaves on semen characteristics in rabbit bucks under tropical climate in Trinidad

2021 ◽  
Author(s):  
Krishna Mohan Kumar
Keyword(s):  
Sperm Motility ◽  
Moringa Oleifera ◽  
Sperm Count ◽  
Control Group ◽  
Computer Assisted ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Moringa Oleifera Leaves ◽  
The Impact

Objective This study aimed to evaluate the impact of the dietary supplement of Moringa oleifera leaves (MOL) on semen quality and characteristics in rabbits. Methods Eighteen (n=18) breeding bucks of New Zealand white, of similar age group, were used for the study. Three feeding regimes, (i) 100% commercial rabbit pellets (CRP)-Group I (ii) 90% CRP + 10% fresh MOL on a dry matter (DM) basis – Group II and (iii) 80% CRP + 20% fresh MOL on a DM basis – Group III, were adopted and the trial continued for 21 days. After adaptation to the diet, semen was collected from each buck and subjected to evaluation using a computer-assisted semen analyser. Results In Group III, the sperm count, normal sperm morphology, and sperm motility increased (52.0%) in comparison with the control (Group I; 50.1%). The inclusion of 20% Moringa oliefera in the diet (Group III) caused a significant increase (P<0.05) in semen concentration (Control =136.2 M/mL; Group III=297.2 M/mL). There was no significant difference (P>0.05) in sperm motility and semen volume among the groups. Conclusion The results suggest that supplementing commercial rabbit pellets with 20% fresh Moringa oliefera leaves on a DM basis can improve the quality and characteristics of semen in breeding bucks.

scholarly journals Doxorubicin induced histomorphometric changes in testes of albino rat

2015 ◽  
Vol 3 (1) ◽  
pp. 10-14 ◽  
Author(s):  
Surendra Kumar Sah ◽  
Saroj Khatiwada ◽  
Deepak Chaudhary ◽  
Chandra Bhushan Jha ◽  
Soumya Bhattacharya
Keyword(s):  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats ◽  
Testis Weight ◽  
Short Term ◽  
Albino Rat ◽  
Left Testis ◽  
Significant Difference ◽  
Experimental Group

Anticancer drugs like doxorubicin have been found to affect male gonads thereby leading to infertility. This study was conducted to evaluate the effects of doxorubicin over short, mid and long term on testes of male albino rats. Sixty male albino rats aged 6-8 weeks were taken for study. The rats were randomly divided into 3 groups of experimental (each group containing 10 rats) and 3 groups of control (each group containing 10 rats). The experimental groups were given a single dose of doxorubicin i.e. 10 mg/kg body weight intra-peritoneally and sacrificed after 3 different duration for each group (second week, eighth week and sixteenth week). All rats under 3 control groups were given a single intra-peritoneal dose of 2.5 ml/kg body weight normal saline and sacrificed with their respective experimental groups. Significant difference in diameters (p=0.029) and cross-sectional area (p=0.028) of seminiferous tubules was observed between short term experimental and short term control rats. For both between midterm experimental and midterm control group, and between long term experimental and long term control group, a significant difference in right testis weight (p<0.001 for both), left testis weight (p<0.001 for both), volume of testis (p<0.001 and p=0.038), diameter (p<0.001 for both) and area (p<0.001 for both) of seminiferous tubules was observed. As compared to short term experimental group, midterm experimental group and long term experimental group had significantly lower right testis weight (p<0.001 for both), left testis weight (p<0.001 for both), diameter of seminiferous tubule (p<0.001 for both) and cross-sectional area of seminiferous tubule (p<0.001 both). Cross-sections of the seminiferous tubules of all the control groups had normal architecture. However, there was progressive destruction of seminiferous tubules structure across the experimental groups. Doxorubicin has deleterious effect on seminiferous tubules of albino rat testis.Nepal Journal of Biotechnology. Dec. 2015 Vol. 3, No. 1: 10-14

scholarly journals Beneficial Role of Ginger Powder (Zingiber officinale) against Acephate-induced Reprotoxicity in Adult Male Rats

2021 ◽  
pp. 50-59
Author(s):  
V. Madhavi ◽  
U. Kanchana Ganga ◽  
S. B. Sainath ◽  
B. Kishori
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Sperm Count ◽  
Membrane Integrity ◽  
Male Rats ◽  
Sperm Viability ◽  
Marker Enzymes ◽  
Group I ◽  
Sperm Membrane

Aims: The present study was aimed to investigate the protective role of ginger against acephate-induced testicular toxicity in adult rats. Methodology: Rats were allocated into four groups where animals in group I served as controls, while animals in group II, III and group IV were treated as experimental rats. Rats in groups II, III and IV were treated with acephate (50mg/kg body weight), ginger (100mg/kg body weight) and combination of both acephate and ginger, respectively over a period of 60 days. After completion of experimental period sperm count, sperm viability, sperm motility, sperm membrane integrity, testicular steroidogenic marker enzymes (3β-HSD and 17β-HSD, serum testosterone and testicular architecture was performed in both control and experimental rats. Results: Relative weights of reproductive organs, sperm count, sperm viability, sperm motility and sperm membrane integrity were significantly decreased in acephate treated rats over controls. Acephate administration also reduced the circulatory levels of testosterone associated with a significant reduction in the testicular steroidogenic marker enzymes (3β-HSD and 17β-HSD) in rats. The testicular architecture was disrupted in acephate intoxicated rats. In contrast, ginger administration significantly recovered the acephate-induced suppressed selected reproductive parameters with increased circulatory levels of testosterone and restoration of sperm endpoints in as compared to acephate alone treated rats. No significant changes were observed in any of the selected reproductive endpoints in ginger treated rats as compared to controls. Conclusion: The results can be concluded that supplementation of ginger mitigates the negative effects of acephate on male reproductive health via amelioration of testicular setroidogenesis and spermatogenesis and epididymal sperm maturation events in rats.

scholarly journals EFFECT OF SECANG WOOD (CEASALPINIA SAPPAN L.) EXTRACT ON MORPHOLOGY OF SPERMATOZOA, SPERM COUNT, AND REVERSIBLE PROCESS IN MALE RATS

2017 ◽  
Vol 3 (2) ◽  
pp. 142-147
Author(s):  
Silfia Sekar Arum ◽  
Syarief Thaufik Hidayat ◽  
Nur Khafidhoh ◽  
Suryati Kumorowulan ◽  
Suhartono Suhartono ◽  
...  
Keyword(s):  
Sperm Morphology ◽  
Sperm Count ◽  
Scientific Information ◽  
Male Rats ◽  
Control Group ◽  
P Value ◽  
Chloroform Fraction ◽  
Reversible Process ◽  
Water Fraction ◽  
Significant Difference

Background: Limited choice of contraception for males is one of the reasons why their participation is low. Secang wood is considered as an alternative contraception that has an influence in the morphology and number of spermatozoa, and reversible in nature.Objective: To examine the effect of extracts of secang wood in the morphology of spermatozoa, sperm count and reversible process in male mice.Methods: This was a quasy experimental study with randomized post test only control group design. There were 32 male rats (Mus Muscullus L) recruited in this study. Four groups were involved, namely: 1) Ethanol group, 2) Chloroform fraction, 3) Water fraction, and 4) Control group. Each group consisted of 8 mice. There were two treatments in this study: 1) each group was given secang extract with dose 50 mg/25 gram of weight, 2) The observation period was 10 days after the treatment, and 4 mice in each group was dissected to see the morphology and sperm count, while the other 4 mice were allowed to live and maintained until 20 days to see reversible morphology and sperm count Results: The extract of sepang wood at a dose of 50mg/25gram weight could increase the abnormal sperm morphology, and lower sperm count. There was a significant difference between the treatment group and control group with p-value <0.05. The extract was also reversible on the morphology and sperm count.Conclusion: There was a significant effect of secang wood extract on sperm morphology, the number of spermatozoa, and reversible process. This study provides the insight of scientific information about the effect of the sepang extracts on the number and morphology of spermatozoa, and it could be used as a basis for further research in human in the development of natural contraceptive on a reversible man.

scholarly journals Effect of Ingested Foods Preheated (With Microwave) in Plastic Containers on the Reproductive Profile of Male Albino Rats

2020 ◽  
pp. 71-75
Author(s):  
Onengiyeofori Ibama ◽  
Ugochukwu Chioma ◽  
Joshua Lelesi Konne ◽  
Felix Konne
Keyword(s):  
Sperm Count ◽  
Semen Analysis ◽  
Microwave Energy ◽  
Male Rats ◽  
Albino Rats ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Group Ii ◽  
Plastic Containers

Recently, there has been a notably increased use of microwave energy for heating and processing foods particularly in catering services and in our homes owing to its speed, convenience and efficiency compared to the traditional heating method. Due to the availability and affordability of plastic containers, they are mostly used to contain foods for preheating in a microwave. However, these plastics pose great risk on humans’ health if they get scratched or heated, and leach out chemicals into the contained foods. Also, the microwave energy has been speculated to have posed risks to the health of humans considering the electromagnetic radiations it emits. This study was aimed at evaluating the effect of ingested foods preheated (with microwave) in plastic containers on the reproductive profile of male albino rats. A total of twenty-four (24) male albino rats weighing between 120 to 200 g were used for this study. The rats were divided into three (3) groups (group I, II and III) of eight (8) rats per group. The rats were fed ad libitum with porridge beans and jellof rice with meat and fish daily for 40 days. The foods ingested by group I rats were not preheated in a microwave; the foods ingested by group II rats were put in ceramics and preheated in a microwave for 2 minutes daily; while the foods ingested by group III rats were put in plastic containers (not labeled as ‘‘microwave safe’’) and preheated in a microwave daily for 2 minutes. After an overnight fast, the rats were anaesthetized using chloroform, and blood samples obtained (via cardiac puncture) were used to analyze serum luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone using the ELISA method. The epididymis was also excised and immediately macerated to obtain semen, which was used for semen analysis. The results obtained showed a significantly decreased (p<0.05) mean LH, FSH, testosterone and sperm count in group III (plastic-microwaved) compared to group I and II. Also noted, was a significantly decreased (p<0.05) sperm count in group II compared to group I. However, there was no significant difference in LH, FSH and testosterone between groups I and II. Conclusively, it was evident from this study that, ingestion of foods preheated (with microwave) in plastic containers overtime, may predispose male rats and thus male individuals to dysfunction in their reproductive system which may eventually lead to male infertility. It is therefore, highly recommended that preheating of foods (with microwave) in plastic containers should be highly prohibited, but should rather be done using ceramics.

scholarly journals An Effective Phytoconstituent Aconitine: A Realistic Approach for the Treatment of Trigeminal Neuralgia

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Dilek Çankal ◽  
Esra Küpeli Akkol ◽  
Yeliz Kılınç ◽  
Mert İlhan ◽  
Raffaele Capasso
Keyword(s):  
Spinal Cord ◽  
Trigeminal Neuralgia ◽  
Motor Coordination ◽  
Infraorbital Nerve ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Significant Difference ◽  
Wistar Albino Rats ◽  
Dose Dependent

Trigeminal neuralgia pain remains a challenge to treat. Natural compounds may be promising options for relieving pain. This study was aimed at investigating the effects of aconitine in a rat model of trigeminal neuralgia pain. Infraorbital nerve chronic constriction injury was performed in adult Wistar Albino rats. After the neuropathic pain developed, the rats were assigned to one of the treatment groups: carbamazepine 40 or 80 mg/kg; aconitine 0.25, 0.50, or 0.75 mg/kg; or saline injection (control group). Behavioral testing with von Frey filaments and the rotarod test were carried out before the surgical procedure and on the 24th to 29th postoperative days. Following the completion of tests, ipsilateral and contralateral spinal cords were harvested for Western blot analyses to assess NR-1 protein expression. ANOVA followed by Mann-Whitney U test was performed for the statistical analyses. P values of <0.05 were considered significant. Aconitine significantly reduced mechanical sensitivity in a dose-dependent manner. A significant reduction in motor coordination was noted for the higher doses of aconitine which was similar with the 40 and 80 mg/kg doses of carbamazepine. NR-1 expression was reduced in the ipsilateral spinal cord, whereas no significant difference was noted between the groups in the expression of NR-1 in the contralateral spinal cord. Aconitine had a significant pain relieving effect, which was similar to carbamazepine, in a dose-dependent manner. Aconitine may be an alternative pharmacological agent for the control of trigeminal neuralgia pain.

scholarly journals Reproductive toxicity potentials of methanolic extract of Portulaca oleracea in male rats: An experimental study

2021 ◽  
Author(s):  
Izuchukwu Azuka Okafor ◽  
Uchenna Somtochukwu Nnamah ◽  
Selasie Ahiatrogah ◽  
Dorcas Serwaa ◽  
Jude Nnaka
Keyword(s):  
Sperm Motility ◽  
Methanolic Extract ◽  
Sperm Count ◽  
Reproductive Toxicity ◽  
Portulaca Oleracea ◽  
Male Rats ◽  
Control Group ◽  
Average Weight ◽  
Male Wistar Rats ◽  
Group B

Background: Purslane is an edible widely distributed shrub and one of the herbs used in decoctions for the treatment of different ailments including infertility. However, there is a shortage of evidence to validate its reproductive effects. Objective: To investigate the effect of methanolic extract of Portulaca oleracea (MEPO) on the reproductive system of male rats. Materials and Methods: Fifteen 10-wk old male Wistar rats with an average weight of 183 gr were randomly divided into three groups (n = 5/each). Group A (the control group) received distilled water only; group B received 400 mg/kg MEPO; and group C received 800 mg/kg MEPO for 14 days. The animals fasted overnight after the 14th day of administration and euthanized by cervical dislocation. Blood samples, sperm, testes, and epididymis were collected for serum hormones, sperm, and histological analyses. Results: There was no significant change in the serum luteinizing hormone and testosterone levels across all groups when compared to the control. However, group C showed a significant increase (p = 0.020) in follicle-stimulating hormone levels when compared to the control. There was a significant reduction (p = 0.006) in the sperm count in group C when compared with the control group. There was also a significantly reduced (p = 0.003) sperm motility in MEPO-treated groups compared to the control. While the testis showed no abnormalities in its histoarchitecture across groups, the epididymis showed some blood congestion in MEPO-treated groups. Conclusion: Portulaca oleracea showed the ability to reduce sperm count and motility at higher doses. Key words: Portulaca oleracea, Purslane, Testis, Epididymis, Rat, Sperm motility.

scholarly journals Pharmacological evaluation of Vrishya karma (Aphrodisiac activity) of Whole plant of Lavandula bipinnata (Roth) Kuntze

2021 ◽  
Vol 12 (3) ◽  
pp. 529-533
Author(s):  
Narendra Singh Dhaked ◽  
Mukesh B Nariya ◽  
Acharya R N
Keyword(s):  
Serum Testosterone ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Albino Rats ◽  
Whole Plant ◽  
Significant Difference ◽  
Weight Test ◽  
Annual Herb ◽  
Anxiety Depression

Background: Lavandula bipinnata Roth. of family Lamiaceae known as Kamaraja in Odisha is an erect, strongly fragrant, annual herb with multifaceted ethno medicinal claims to treat, poisons, tooth ache, anxiety, depression, headache, cold and sexual disorder etc. Aim: To study the effect of whole plant of Lavandula bipinnata for its aphrodisiac activity in albino rats. Material Methods: The sexually active male rats were chosen separately and divided into 2 groups; each group consisting of 6 animals and the study was carried by using the suitable animal experimental model. The data generated during the study were analyzed by employing Student’s‘t’ test for paired and unpaired data as applicable to determine significant difference between groups at P<0.05. Results: Lavandula bipinnata showed significant increase in weight of rats in comparison with initial weight. Test drug provided significant increase in mounting frequency, licking, chasing, genital sniffing, in comparison to control group and non-significant increase in mounting latency and serum testosterone level. Conclusion: Lavandula bipinnata whole plant possess significant aphrodisiac activity in test rat model and confirms its traditional claim as an aphrodisiac. 

Sign in / Sign up

Export Citation Format

Share Document